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The map label for this gene is fus [H]

Identifier: 18311390

GI number: 18311390

Start: 2754585

End: 2756651

Strand: Reverse

Name: fus [H]

Synonym: CPE2408

Alternate gene names: 18311390

Gene position: 2756651-2754585 (Counterclockwise)

Preceding gene: 18311391

Following gene: 18311389

Centisome position: 90.94

GC content: 37.4

Gene sequence:

>2067_bases
ATGGCTAGACAATATCCGTTAGAAAAATTCCGTAACTTCGGAATAATGGCACATATAGATGCTGGTAAAACAACTACTAC
TGAGCGTATTCTTTTCTATACAGGAAGAAACCACAAAATAGGGGAAACTCATGATGGAGCTTCAACTATGGACTGGATGG
CTCAAGAGCAAGAAAGAGGTATAACAATAACTTCTGCTGCTACAACTTGTTTCTGGAAAGGTTATGAATTAAACATAATC
GATACTCCAGGACACGTAGACTTCACAGTTGAGGTTGAAAGATCATTAAGAGTTCTTGATGGAGCTGTTACTGTTCTTGA
TGCTAAGAGTGGAGTTGAACCACAAACTGAAACTGTTTGGAGACAGGCAGACAAGTACGGCGTTCCAAGAATGATATACG
TAAACAAAATGGATGCTACAGGAGCAGACTACTACAACTGTATAAACACAGTTAGAGAAAGATTACAAGCTAATGCTGTT
GCAATCCAAATTCCAATAGGTCAAGAAGATCAATTCCAAGGAATGGTTGATTTATTAACTAACCAAGCAATAATCTTCAA
AGATGACTTAGGAAAAGACATAGAAGTAGGTGAAGTTCCAGCTGATTTAGCTGATAAAGCAGAAGAGTATAGAGCTGCTA
TGATCGAAGCTATAGCTGAAACTGATGAAGAGTTAATGATGAAATACTTAGAAGGTGAAGAATTAACTCTTGAAGAATTA
AAAGTTGCTTTAAGAAAAGCTACTATAAACAATGAAATAATCCCAGTTATCTGTGGATCATCATACAAAAACAAAGGTGT
TCAACAAATGATAGATGGTGTTGTTGATTACTTACCATCACCATTAGATATACCTGCTGTTAAAGGTACTAACTTAGACG
GTGAAGAAGAAGTTAGAGAAGCTTCAGATGACGCTCCAATGTCAGCTTTAGCATTCAAAATAGCTACTGACCCATTCGTT
GGAAGATTAGCATTCACAAGAGTTTACTCAGGAGTTTTAGAGAGCGGTTCATACGTATTAAACTCAACTAAGGGTAAAAA
AGAAAGAATCGGAAGACTTGTTAAGATGCACGCTAACTCAAGAGAGGAAGTTGAATCATTAGAAGCAGCTGAATTAGGAG
CTGTAATAGGTCTTAAGAACACAACTACTGGAGATACTTTATGTACAGAAGCAGCTCCAATAATACTTGAAAAGATGGAA
TTCCCAGAGCCAGTTATATCTATAGCTATCGAGCCAAAGACAAAAGCTGGTCAAGAAAAAATGGGTATAGCTTTATCAAA
GCTTGCTGAAGAGGATCCAACTTTCAAAACTTGGACTGATCAAGAAACAGGTCAAACTATCATAGCTGGTATGGGTGAGC
TTCACTTAGATATCATCGTTGATAGATTACAAAGAGAATTCAAAGTTGAGTGTAACGTAGGTGCTCCTCAAGTTGCTTAC
AAAGAAACTATCAAAAAGGCTGTTGAAGCAGAAGCTAAATTTGCTAGACAATCTGGTGGTAGAGGACAATACGGTCACTG
TAAGATAGAAATGATACCAACTGAAGGCGAATATGAATTCGAAAATGCTATCGTTGGAGGAGCTATTCCAAGAGAATACA
TTCCAGCAGTAGATAACGGAATCAGAGAAGCTGCAGAAAGTGGTATAATAGCTGGATACCCAGTTATAAACTTCAAAATA
AGATTATTCGACGGATCATACCATGATGTCGATTCATCTGAAATGGCATTCAAAATAGCTGGATCTATGGCATTCAAAAA
CGCTATGGCTAAAGCTGATGCTGTATTACTTGAGCCTATAATGAAAGTTGAAATCACTGTACCAGAAGAGTACATGGGAG
ACGTTATAGGAGATGTTAACTCAAGAAGAGGTAGAATGGAAGGAATGGACTCAAGAAATGGTGCACAAATCATAAGAGCA
TTCATCCCACTATCAGAAATGTTTGGATACGCAACTGCATTAAGATCAAGAACTCAAGGTAGAGGAACTTATGCAATGGA
ATTCGATCACTATGATGACGTTCCTAAGAGCATCCAAGAAGAAGTTGCAGGTAAAAAAAATAAATAA

Upstream 100 bases:

>100_bases
TTGCTCATTACAGATATTAATAAAATGAAACTGTTTTGGCTAAAAGCCAAAACAGTTTTGTTGAATTTAAAAACACTATA
CGTTGAGAGGAGGAGTCACA

Downstream 100 bases:

>100_bases
TTTAAAGAAGCTTTAAAAGCTTCTTTAATATATAAATGTATATAATATTTTTAAATTAAAGGAGGAATTTGCAATGTCAA
AAGCAAAATTTGAAAGAAGC

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MARQYPLEKFRNFGIMAHIDAGKTTTTERILFYTGRNHKIGETHDGASTMDWMAQEQERGITITSAATTCFWKGYELNII
DTPGHVDFTVEVERSLRVLDGAVTVLDAKSGVEPQTETVWRQADKYGVPRMIYVNKMDATGADYYNCINTVRERLQANAV
AIQIPIGQEDQFQGMVDLLTNQAIIFKDDLGKDIEVGEVPADLADKAEEYRAAMIEAIAETDEELMMKYLEGEELTLEEL
KVALRKATINNEIIPVICGSSYKNKGVQQMIDGVVDYLPSPLDIPAVKGTNLDGEEEVREASDDAPMSALAFKIATDPFV
GRLAFTRVYSGVLESGSYVLNSTKGKKERIGRLVKMHANSREEVESLEAAELGAVIGLKNTTTGDTLCTEAAPIILEKME
FPEPVISIAIEPKTKAGQEKMGIALSKLAEEDPTFKTWTDQETGQTIIAGMGELHLDIIVDRLQREFKVECNVGAPQVAY
KETIKKAVEAEAKFARQSGGRGQYGHCKIEMIPTEGEYEFENAIVGGAIPREYIPAVDNGIREAAESGIIAGYPVINFKI
RLFDGSYHDVDSSEMAFKIAGSMAFKNAMAKADAVLLEPIMKVEITVPEEYMGDVIGDVNSRRGRMEGMDSRNGAQIIRA
FIPLSEMFGYATALRSRTQGRGTYAMEFDHYDDVPKSIQEEVAGKKNK

Sequences:

>Translated_688_residues
MARQYPLEKFRNFGIMAHIDAGKTTTTERILFYTGRNHKIGETHDGASTMDWMAQEQERGITITSAATTCFWKGYELNII
DTPGHVDFTVEVERSLRVLDGAVTVLDAKSGVEPQTETVWRQADKYGVPRMIYVNKMDATGADYYNCINTVRERLQANAV
AIQIPIGQEDQFQGMVDLLTNQAIIFKDDLGKDIEVGEVPADLADKAEEYRAAMIEAIAETDEELMMKYLEGEELTLEEL
KVALRKATINNEIIPVICGSSYKNKGVQQMIDGVVDYLPSPLDIPAVKGTNLDGEEEVREASDDAPMSALAFKIATDPFV
GRLAFTRVYSGVLESGSYVLNSTKGKKERIGRLVKMHANSREEVESLEAAELGAVIGLKNTTTGDTLCTEAAPIILEKME
FPEPVISIAIEPKTKAGQEKMGIALSKLAEEDPTFKTWTDQETGQTIIAGMGELHLDIIVDRLQREFKVECNVGAPQVAY
KETIKKAVEAEAKFARQSGGRGQYGHCKIEMIPTEGEYEFENAIVGGAIPREYIPAVDNGIREAAESGIIAGYPVINFKI
RLFDGSYHDVDSSEMAFKIAGSMAFKNAMAKADAVLLEPIMKVEITVPEEYMGDVIGDVNSRRGRMEGMDSRNGAQIIRA
FIPLSEMFGYATALRSRTQGRGTYAMEFDHYDDVPKSIQEEVAGKKNK
>Mature_687_residues
ARQYPLEKFRNFGIMAHIDAGKTTTTERILFYTGRNHKIGETHDGASTMDWMAQEQERGITITSAATTCFWKGYELNIID
TPGHVDFTVEVERSLRVLDGAVTVLDAKSGVEPQTETVWRQADKYGVPRMIYVNKMDATGADYYNCINTVRERLQANAVA
IQIPIGQEDQFQGMVDLLTNQAIIFKDDLGKDIEVGEVPADLADKAEEYRAAMIEAIAETDEELMMKYLEGEELTLEELK
VALRKATINNEIIPVICGSSYKNKGVQQMIDGVVDYLPSPLDIPAVKGTNLDGEEEVREASDDAPMSALAFKIATDPFVG
RLAFTRVYSGVLESGSYVLNSTKGKKERIGRLVKMHANSREEVESLEAAELGAVIGLKNTTTGDTLCTEAAPIILEKMEF
PEPVISIAIEPKTKAGQEKMGIALSKLAEEDPTFKTWTDQETGQTIIAGMGELHLDIIVDRLQREFKVECNVGAPQVAYK
ETIKKAVEAEAKFARQSGGRGQYGHCKIEMIPTEGEYEFENAIVGGAIPREYIPAVDNGIREAAESGIIAGYPVINFKIR
LFDGSYHDVDSSEMAFKIAGSMAFKNAMAKADAVLLEPIMKVEITVPEEYMGDVIGDVNSRRGRMEGMDSRNGAQIIRAF
IPLSEMFGYATALRSRTQGRGTYAMEFDHYDDVPKSIQEEVAGKKNK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=695, Percent_Identity=42.8776978417266, Blast_Score=561, Evalue=1e-160,
Organism=Homo sapiens, GI19923640, Length=724, Percent_Identity=38.8121546961326, Blast_Score=498, Evalue=1e-140,
Organism=Homo sapiens, GI25306287, Length=719, Percent_Identity=34.9095966620306, Blast_Score=411, Evalue=1e-114,
Organism=Homo sapiens, GI25306283, Length=457, Percent_Identity=42.0131291028446, Blast_Score=335, Evalue=1e-91,
Organism=Homo sapiens, GI217272892, Length=797, Percent_Identity=24.3412797992472, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI217272894, Length=797, Percent_Identity=24.3412797992472, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI157426893, Length=199, Percent_Identity=33.6683417085427, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI4503483, Length=146, Percent_Identity=40.4109589041096, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI94966754, Length=160, Percent_Identity=35, Blast_Score=93, Evalue=1e-18,
Organism=Homo sapiens, GI310132016, Length=119, Percent_Identity=36.9747899159664, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI310110807, Length=119, Percent_Identity=36.9747899159664, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI310123363, Length=119, Percent_Identity=36.9747899159664, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI94966752, Length=90, Percent_Identity=37.7777777777778, Blast_Score=70, Evalue=9e-12,
Organism=Escherichia coli, GI1789738, Length=704, Percent_Identity=62.0738636363636, Blast_Score=884, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=483, Percent_Identity=29.1925465838509, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI48994988, Length=136, Percent_Identity=42.6470588235294, Blast_Score=113, Evalue=5e-26,
Organism=Escherichia coli, GI1788922, Length=142, Percent_Identity=41.5492957746479, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=692, Percent_Identity=40.3179190751445, Blast_Score=507, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17556745, Length=722, Percent_Identity=28.808864265928, Blast_Score=324, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17552882, Length=787, Percent_Identity=22.3634053367217, Blast_Score=116, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17506493, Length=475, Percent_Identity=24.2105263157895, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17557151, Length=141, Percent_Identity=40.4255319148936, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=35.0746268656716, Blast_Score=83, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=35.0746268656716, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323098, Length=687, Percent_Identity=42.0669577874818, Blast_Score=546, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6322359, Length=774, Percent_Identity=32.5581395348837, Blast_Score=395, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324707, Length=530, Percent_Identity=24.7169811320755, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6320593, Length=530, Percent_Identity=24.7169811320755, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323320, Length=146, Percent_Identity=39.041095890411, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324166, Length=143, Percent_Identity=34.2657342657343, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582462, Length=690, Percent_Identity=41.1594202898551, Blast_Score=550, Evalue=1e-156,
Organism=Drosophila melanogaster, GI221458488, Length=726, Percent_Identity=32.6446280991736, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78706572, Length=142, Percent_Identity=40.1408450704225, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24585711, Length=447, Percent_Identity=24.6085011185682, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24585713, Length=447, Percent_Identity=24.6085011185682, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24585709, Length=447, Percent_Identity=24.6085011185682, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI28574573, Length=211, Percent_Identity=28.9099526066351, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357743, Length=141, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 75988; Mature: 75857

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQYPLEKFRNFGIMAHIDAGKTTTTERILFYTGRNHKIGETHDGASTMDWMAQEQERG
CCCCCCHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
ITITSAATTCFWKGYELNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAKSGVEPQTETVW
EEEEECCCEEEECCEEEEEEECCCCEEEEEEHHHHHHHHHCEEEEEECCCCCCCHHHHHH
RQADKYGVPRMIYVNKMDATGADYYNCINTVRERLQANAVAIQIPIGQEDQFQGMVDLLT
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHH
NQAIIFKDDLGKDIEVGEVPADLADKAEEYRAAMIEAIAETDEELMMKYLEGEELTLEEL
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHH
KVALRKATINNEIIPVICGSSYKNKGVQQMIDGVVDYLPSPLDIPAVKGTNLDGEEEVRE
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
ASDDAPMSALAFKIATDPFVGRLAFTRVYSGVLESGSYVLNSTKGKKERIGRLVKMHANS
CCCCCCHHHHEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCC
REEVESLEAAELGAVIGLKNTTTGDTLCTEAAPIILEKMEFPEPVISIAIEPKTKAGQEK
HHHHHHHHHHHHCEEEECCCCCCCCHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCHHH
MGIALSKLAEEDPTFKTWTDQETGQTIIAGMGELHLDIIVDRLQREFKVECNVGAPQVAY
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHEEEEECCCCCHHHH
KETIKKAVEAEAKFARQSGGRGQYGHCKIEMIPTEGEYEFENAIVGGAIPREYIPAVDNG
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHCEEECCCCCHHHCCHHHHH
IREAAESGIIAGYPVINFKIRLFDGSYHDVDSSEMAFKIAGSMAFKNAMAKADAVLLEPI
HHHHHHCCCEECCEEEEEEEEEECCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHCCE
MKVEITVPEEYMGDVIGDVNSRRGRMEGMDSRNGAQIIRAFIPLSEMFGYATALRSRTQG
EEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCC
RGTYAMEFDHYDDVPKSIQEEVAGKKNK
CCEEEEECCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARQYPLEKFRNFGIMAHIDAGKTTTTERILFYTGRNHKIGETHDGASTMDWMAQEQERG
CCCCCHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
ITITSAATTCFWKGYELNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAKSGVEPQTETVW
EEEEECCCEEEECCEEEEEEECCCCEEEEEEHHHHHHHHHCEEEEEECCCCCCCHHHHHH
RQADKYGVPRMIYVNKMDATGADYYNCINTVRERLQANAVAIQIPIGQEDQFQGMVDLLT
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHH
NQAIIFKDDLGKDIEVGEVPADLADKAEEYRAAMIEAIAETDEELMMKYLEGEELTLEEL
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHH
KVALRKATINNEIIPVICGSSYKNKGVQQMIDGVVDYLPSPLDIPAVKGTNLDGEEEVRE
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
ASDDAPMSALAFKIATDPFVGRLAFTRVYSGVLESGSYVLNSTKGKKERIGRLVKMHANS
CCCCCCHHHHEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCC
REEVESLEAAELGAVIGLKNTTTGDTLCTEAAPIILEKMEFPEPVISIAIEPKTKAGQEK
HHHHHHHHHHHHCEEEECCCCCCCCHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCHHH
MGIALSKLAEEDPTFKTWTDQETGQTIIAGMGELHLDIIVDRLQREFKVECNVGAPQVAY
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHEEEEECCCCCHHHH
KETIKKAVEAEAKFARQSGGRGQYGHCKIEMIPTEGEYEFENAIVGGAIPREYIPAVDNG
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHCEEECCCCCHHHCCHHHHH
IREAAESGIIAGYPVINFKIRLFDGSYHDVDSSEMAFKIAGSMAFKNAMAKADAVLLEPI
HHHHHHCCCEECCEEEEEEEEEECCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHCCE
MKVEITVPEEYMGDVIGDVNSRRGRMEGMDSRNGAQIIRAFIPLSEMFGYATALRSRTQG
EEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCC
RGTYAMEFDHYDDVPKSIQEEVAGKKNK
CCEEEEECCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA