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The map label for this gene is tuf

Identifier: 18311389

GI number: 18311389

Start: 2753318

End: 2754511

Strand: Reverse

Name: tuf

Synonym: CPE2407

Alternate gene names: 18311389

Gene position: 2754511-2753318 (Counterclockwise)

Preceding gene: 18311390

Following gene: 18311388

Centisome position: 90.87

GC content: 39.45

Gene sequence:

>1194_bases
ATGTCAAAAGCAAAATTTGAAAGAAGCAAACCACACGTTAACATTGGAACAATCGGTCACGTAGACCACGGTAAAACAAC
TTTAACAGCAGCTATAACAACAGTTTTAGCACAAGCTGGTGGAGCAGAAGCATTCAAATATGATGAAATAGATAAAGCTC
CAGAAGAAAAAGAAAGAGGAATCACAATCAACACAGCACACGTTGAGTACGAAACAGCTAACAGACACTACGCTCACGTT
GACTGTCCAGGACATGCTGACTACGTTAAAAACATGATCACTGGAGCAGCTCAAATGGACGGAGCTATATTAGTTTGTTC
AGCAGCTGATGGTCCAATGCCTCAAACAAGAGAGCACATCTTATTATCATCAAGAGTTGGAGTTGACCACATCGTAGTAT
TCTTAAACAAAGCAGATATGGTTGACGACGAAGAATTATTAGAATTAGTTGAAATGGAAGTTAGAGAGTTATTAAGCGAG
TACAACTTCCCAGGAGACGACATTCCAGTAATCAAAGGATCAGCTTTAGTAGCATTAGAAAACCCAACTGACGAAGCTGC
AACAGCTTGTATCAGAGAGTTAATGGATGCTGTAGATAGCTACATCCCAACACCAGAAAGAGCAACAGATAAGCCATTCT
TAATGCCAGTAGAGGACGTATTCACAATCACTGGTAGAGGAACAGTTGCAACAGGAAGAGTTGAAAGAGGAGTTCTACAT
GTAGGAGACGAAGTAGAAGTAATCGGATTAACTGAAGAAAGAAGAAAAACAGTTGTAACAGGAATCGAAATGTTCAGAAA
GTTATTAGATGAAGCACAAGCTGGAGATAACATCGGAGCATTATTAAGAGGTATCCAAAGAACTGATATCGAAAGAGGTC
AAGTTTTAGCTCAAGTTGGAACAATCAACCCACACAAAAAATTCGTAGGTCAAGTATACGTACTTAAAAAAGAAGAAGGT
GGAAGACACACTCCATTCTTCGATGGATACAGACCACAATTCTACTTCAGAACAACAGACGTTACAGGATCAATCAAATT
ACCAGAAGGAATGGAAATGGTTATGCCTGGAGACCACATCGACATGGAAGTTGAATTAATCACAGAAATCGCTATGGATG
AAGGATTAAGATTCGCTATCAGAGAAGGTGGAAGAACTGTAGGTTCAGGAGTTGTTACTTCAATAATCGAGTAA

Upstream 100 bases:

>100_bases
GAAGTTGCAGGTAAAAAAAATAAATAATTTAAAGAAGCTTTAAAAGCTTCTTTAATATATAAATGTATATAATATTTTTA
AATTAAAGGAGGAATTTGCA

Downstream 100 bases:

>100_bases
TTTTAACTTTTTAAATAAATTTTTTATTAGTACCGATGAACAACGGGAATTTATGCTTAGTTAAAACTCACTTATATACT
TAGCGCAAAAGTTTATAAGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAQAGGAEAFKYDEIDKAPEEKERGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSSRVGVDHIVVFLNKADMVDDEELLELVEMEVRELLSE
YNFPGDDIPVIKGSALVALENPTDEAATACIRELMDAVDSYIPTPERATDKPFLMPVEDVFTITGRGTVATGRVERGVLH
VGDEVEVIGLTEERRKTVVTGIEMFRKLLDEAQAGDNIGALLRGIQRTDIERGQVLAQVGTINPHKKFVGQVYVLKKEEG
GRHTPFFDGYRPQFYFRTTDVTGSIKLPEGMEMVMPGDHIDMEVELITEIAMDEGLRFAIREGGRTVGSGVVTSIIE

Sequences:

>Translated_397_residues
MSKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAQAGGAEAFKYDEIDKAPEEKERGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSSRVGVDHIVVFLNKADMVDDEELLELVEMEVRELLSE
YNFPGDDIPVIKGSALVALENPTDEAATACIRELMDAVDSYIPTPERATDKPFLMPVEDVFTITGRGTVATGRVERGVLH
VGDEVEVIGLTEERRKTVVTGIEMFRKLLDEAQAGDNIGALLRGIQRTDIERGQVLAQVGTINPHKKFVGQVYVLKKEEG
GRHTPFFDGYRPQFYFRTTDVTGSIKLPEGMEMVMPGDHIDMEVELITEIAMDEGLRFAIREGGRTVGSGVVTSIIE
>Mature_396_residues
SKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAQAGGAEAFKYDEIDKAPEEKERGITINTAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSSRVGVDHIVVFLNKADMVDDEELLELVEMEVRELLSEY
NFPGDDIPVIKGSALVALENPTDEAATACIRELMDAVDSYIPTPERATDKPFLMPVEDVFTITGRGTVATGRVERGVLHV
GDEVEVIGLTEERRKTVVTGIEMFRKLLDEAQAGDNIGALLRGIQRTDIERGQVLAQVGTINPHKKFVGQVYVLKKEEGG
RHTPFFDGYRPQFYFRTTDVTGSIKLPEGMEMVMPGDHIDMEVELITEIAMDEGLRFAIREGGRTVGSGVVTSIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=57.2151898734177, Blast_Score=480, Evalue=1e-136,
Organism=Homo sapiens, GI4503471, Length=452, Percent_Identity=30.3097345132743, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI4503475, Length=449, Percent_Identity=29.3986636971047, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI223555963, Length=447, Percent_Identity=28.8590604026846, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI5729864, Length=447, Percent_Identity=28.8590604026846, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI54607086, Length=314, Percent_Identity=28.9808917197452, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI194018522, Length=317, Percent_Identity=28.7066246056782, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=27.6785714285714, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=27.6785714285714, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI46094014, Length=326, Percent_Identity=29.1411042944785, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI4503507, Length=345, Percent_Identity=28.1159420289855, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI157426893, Length=238, Percent_Identity=27.3109243697479, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI94966754, Length=130, Percent_Identity=33.0769230769231, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=73.7373737373737, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=73.7373737373737, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI2367247, Length=338, Percent_Identity=31.6568047337278, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI48994988, Length=272, Percent_Identity=30.8823529411765, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789108, Length=320, Percent_Identity=30.625, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788922, Length=331, Percent_Identity=25.0755287009063, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=401, Percent_Identity=51.6209476309227, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=44.8453608247423, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=31.5555555555556, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=31.5555555555556, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=431, Percent_Identity=29.9303944315545, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17506081, Length=274, Percent_Identity=31.021897810219, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=441, Percent_Identity=26.530612244898, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=441, Percent_Identity=26.530612244898, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=336, Percent_Identity=28.5714285714286, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=40.1360544217687, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17509919, Length=285, Percent_Identity=27.0175438596491, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=26.5822784810127, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=64.572864321608, Blast_Score=527, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=446, Percent_Identity=29.8206278026906, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=446, Percent_Identity=29.8206278026906, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=373, Percent_Identity=26.2734584450402, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320863, Length=286, Percent_Identity=25.8741258741259, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6320377, Length=342, Percent_Identity=27.4853801169591, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=255, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=57.6142131979695, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=57.6142131979695, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=50.7772020725389, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=30.0668151447661, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=30.0668151447661, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=30.0668151447661, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=30.0668151447661, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24652838, Length=450, Percent_Identity=30.4444444444444, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI17137572, Length=450, Percent_Identity=30.4444444444444, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI45550900, Length=325, Percent_Identity=30.4615384615385, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=31.3725490196078, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI17137380, Length=342, Percent_Identity=30.4093567251462, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24647048, Length=370, Percent_Identity=26.4864864864865, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647046, Length=370, Percent_Identity=26.4864864864865, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_CLOP1 (Q0TMN0)

Other databases:

- EMBL:   CP000246
- EMBL:   CP000246
- RefSeq:   YP_697096.1
- RefSeq:   YP_697110.1
- ProteinModelPortal:   Q0TMN0
- SMR:   Q0TMN0
- STRING:   Q0TMN0
- GeneID:   4201584
- GeneID:   4203723
- GenomeReviews:   CP000246_GR
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_2716
- KEGG:   cpf:CPF_2730
- TIGR:   CPF_2716
- TIGR:   CPF_2730
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- BioCyc:   CPER195103:CPF_2716-MONOMER
- BioCyc:   CPER195103:CPF_2730-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43558; Mature: 43427

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAQAGGAEAFKYDEIDKAPEEKERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLSSRVGVDHIVVFLNKADMVDDEELLELVEMEVRELLSEYNFPGDDIPVIKGSALVALE
EEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
NPTDEAATACIRELMDAVDSYIPTPERATDKPFLMPVEDVFTITGRGTVATGRVERGVLH
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHEEEECCCEEEECCCCCCEEE
VGDEVEVIGLTEERRKTVVTGIEMFRKLLDEAQAGDNIGALLRGIQRTDIERGQVLAQVG
ECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHEEEECC
TINPHKKFVGQVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGSIKLPEGMEMVMPGDHI
CCCHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCC
DMEVELITEIAMDEGLRFAIREGGRTVGSGVVTSIIE
CCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAQAGGAEAFKYDEIDKAPEEKERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLSSRVGVDHIVVFLNKADMVDDEELLELVEMEVRELLSEYNFPGDDIPVIKGSALVALE
EEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
NPTDEAATACIRELMDAVDSYIPTPERATDKPFLMPVEDVFTITGRGTVATGRVERGVLH
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHEEEECCCEEEECCCCCCEEE
VGDEVEVIGLTEERRKTVVTGIEMFRKLLDEAQAGDNIGALLRGIQRTDIERGQVLAQVG
ECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHEEEECC
TINPHKKFVGQVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGSIKLPEGMEMVMPGDHI
CCCHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCC
DMEVELITEIAMDEGLRFAIREGGRTVGSGVVTSIIE
CCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA