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The map label for this gene is topB [H]

Identifier: 18310965

GI number: 18310965

Start: 2269532

End: 2271694

Strand: Reverse

Name: topB [H]

Synonym: CPE1983

Alternate gene names: 18310965

Gene position: 2271694-2269532 (Counterclockwise)

Preceding gene: 18310966

Following gene: 18310959

Centisome position: 74.94

GC content: 30.61

Gene sequence:

>2163_bases
TTGAAAAAGGTAATAATTGCAGAAAAACCATCTGTTGCTAGAAATATTGCAGATGCTTTTAATATAAAAAAGAGAAAAGA
TGGATACTTTGAAGGCGAAGAATATCTTATAACATGGGCTTTCGGACACTTATTGCAGTTATTTGATGCAAAGGATTATG
ATGAGAACATGAAGGGATGGAGAATGGAGAAATTCCCTTTTATCCCAGAAGTCTTTAAATATAAAATAAAACAAGATTCT
TTAAATAGAAACATCATTGATAAGGGTGCGGAAAAGCAAATAAAGATAATAAAAGGTCTTATAGATAGGGAAGATGTAGA
TGGAATAATTTCTGCTACTGACTTTGATAGAGAGGGACAAGTAATTGCAGATGAAATTTTCCTATATTTTAGTGAAAAGA
AACCAGTATATAGACTTTTATTAAATGAATGGACACCAGATGAAGTTAAAAAAGGAATGGAAAAGTTAAAACCTAATGAG
GAAATGCAATCTCTTCAAGATGCTGGTATAGGAAGACAGTGGGCAGACTGGATAATAGGTATAAATTTAACTTCTGTGAC
AACAATCAAATATAAGCTAAATGATGGACAGATTTTAAATATAGGGAGAGTTCTTCTACCTACTTTAAAAATAATTTATG
ACAGAGATAAAGAAATAGAGAACTTTAAATCAAGTGCATATTACAAACTTAATGGTACTTTTAAGACTTCAAAAAATGAG
GAGTTTGAAGGAACTTATTATGAAAATAATTCAGAGAAGTTTGAAGATAAAAAGGTTTTAGATGAGATTAAAGAAAAGAT
AAAGGATAAAAATGCTGAAATAATTGAAAAGCAAACAGAAAGAAAAAAAGAATATCCACCATTTTTATTTAATCTTTCTA
ATCTTCAAGGATACATAACAAGTAAATACAAAGGATGGACTTCAGATAAGGTTTTAAAAGTAGCTCAATCTTTATATGAA
AAGAAGTTTATAACTTATCCTAGAACAGGAAGTATGGCTCTAGAGGAGAGCTTAGTTGATAGAACTAGAAATGTTTTAGA
AAAGCTTAAGGTAGGCTTGCCTTATGAGGAGCAAATAAAGTTTGTTCAAAATAAAAGGGTTTTTGATAATTCAAAGGTTG
AGAGCCATAGTGCCATAGTTCCAACTTACATGAAACCTAATAATTTAACTCAAGATGAGAGAATAGTTTATACAGCAGTT
AAAAATAGATTTCTTATGCAATTTATGCCTGTAGCTGAATTTGAGGAAACAAAAATAACTTCTAAGATAAATGATAAAGA
GGTTAAGGGCGTATTTATTTCAAAGGGAAAAGTTCAGCTTATAGAAGGATGGAGAGTTGTTGAAAAAATAGATAGTAAAG
ATACAATTCTTCCAATGGTAAATGAAAAAGAAATAGTGGATTTAGTAGATAGTAAAATAAATAAGGTTACTAAAAAACCA
CCTAAAAAGCATACTGAGAAAACTTTGCTTAGGGTAATGGAGACTTGTGGCAAGAGTTTTAAAGAGGATGCAGAGGAAGA
TAGTGAAGAAATGATGGCTTCAATATTAAGTGGATTTAGTATAGGAACACCTGCTACTAGAGCTGAAACAATTAAAAAGC
TTAAGGATGTAGGATATATTACCACAAAGGGAAAAAGTTTAACTTGTACAGAGCTTGGTAGAACCATAGTTGAAATATTC
CCTGTTAAGGAACTTTTAGATTTAGAATACACAGGAAGACTAGAAAAAACTCTTTCTGATATAGAAAGAGGGAAGGTTAA
AAAAGAAGATTTCTTAAATCTTATAAAAAACTTTACTATAGAGGGAGTTAAGGCTATAAAAAATGATGATTCCATGGCAA
AACACTTTAAAGTAGAGGCTCCAGAGGGAGTAGAGGTTTTAGGAAAATGTCCTAACTGTGGAAATCCTGTTATAGAGGGA
GAAAGAGGATTTGGATGTGTTAACTGGAAAAATGGATGTAAATATACCATATGGAAAAATGACAAGTATATAGCTTCCTT
TGGAAAACAAGTAACACCTCAGATGGTAAAACTTCTATTAGCTAATGGAAAAGTCGGATTTAGAAATTTAAAAAGTAAAA
AGGGAAATACCTTTGCAGCATACTTCATATATGAAAAAGATGAAGAAAAAGGTTATTTCACATGGAAAATGGAATTTATT
TAA

Upstream 100 bases:

>100_bases
TTTTAATAAGTGAAATAAATTTTTAAATCTGTCAAAGATATAGAAGAGGAGAGAATATAGAAGTTAATATAACAAAAGGA
TTATAATTAGGAGTGATACT

Downstream 100 bases:

>100_bases
TAAAATATATATGAGTTGTATTAAAAGAAATAAAAATGTTATGTAGCAAAATATAATAATAAAACTTACAAACACATTCA
GAGAATTACTAGATTTTCCT

Product: DNA topoisomerase

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MKKVIIAEKPSVARNIADAFNIKKRKDGYFEGEEYLITWAFGHLLQLFDAKDYDENMKGWRMEKFPFIPEVFKYKIKQDS
LNRNIIDKGAEKQIKIIKGLIDREDVDGIISATDFDREGQVIADEIFLYFSEKKPVYRLLLNEWTPDEVKKGMEKLKPNE
EMQSLQDAGIGRQWADWIIGINLTSVTTIKYKLNDGQILNIGRVLLPTLKIIYDRDKEIENFKSSAYYKLNGTFKTSKNE
EFEGTYYENNSEKFEDKKVLDEIKEKIKDKNAEIIEKQTERKKEYPPFLFNLSNLQGYITSKYKGWTSDKVLKVAQSLYE
KKFITYPRTGSMALEESLVDRTRNVLEKLKVGLPYEEQIKFVQNKRVFDNSKVESHSAIVPTYMKPNNLTQDERIVYTAV
KNRFLMQFMPVAEFEETKITSKINDKEVKGVFISKGKVQLIEGWRVVEKIDSKDTILPMVNEKEIVDLVDSKINKVTKKP
PKKHTEKTLLRVMETCGKSFKEDAEEDSEEMMASILSGFSIGTPATRAETIKKLKDVGYITTKGKSLTCTELGRTIVEIF
PVKELLDLEYTGRLEKTLSDIERGKVKKEDFLNLIKNFTIEGVKAIKNDDSMAKHFKVEAPEGVEVLGKCPNCGNPVIEG
ERGFGCVNWKNGCKYTIWKNDKYIASFGKQVTPQMVKLLLANGKVGFRNLKSKKGNTFAAYFIYEKDEEKGYFTWKMEFI

Sequences:

>Translated_720_residues
MKKVIIAEKPSVARNIADAFNIKKRKDGYFEGEEYLITWAFGHLLQLFDAKDYDENMKGWRMEKFPFIPEVFKYKIKQDS
LNRNIIDKGAEKQIKIIKGLIDREDVDGIISATDFDREGQVIADEIFLYFSEKKPVYRLLLNEWTPDEVKKGMEKLKPNE
EMQSLQDAGIGRQWADWIIGINLTSVTTIKYKLNDGQILNIGRVLLPTLKIIYDRDKEIENFKSSAYYKLNGTFKTSKNE
EFEGTYYENNSEKFEDKKVLDEIKEKIKDKNAEIIEKQTERKKEYPPFLFNLSNLQGYITSKYKGWTSDKVLKVAQSLYE
KKFITYPRTGSMALEESLVDRTRNVLEKLKVGLPYEEQIKFVQNKRVFDNSKVESHSAIVPTYMKPNNLTQDERIVYTAV
KNRFLMQFMPVAEFEETKITSKINDKEVKGVFISKGKVQLIEGWRVVEKIDSKDTILPMVNEKEIVDLVDSKINKVTKKP
PKKHTEKTLLRVMETCGKSFKEDAEEDSEEMMASILSGFSIGTPATRAETIKKLKDVGYITTKGKSLTCTELGRTIVEIF
PVKELLDLEYTGRLEKTLSDIERGKVKKEDFLNLIKNFTIEGVKAIKNDDSMAKHFKVEAPEGVEVLGKCPNCGNPVIEG
ERGFGCVNWKNGCKYTIWKNDKYIASFGKQVTPQMVKLLLANGKVGFRNLKSKKGNTFAAYFIYEKDEEKGYFTWKMEFI
>Mature_720_residues
MKKVIIAEKPSVARNIADAFNIKKRKDGYFEGEEYLITWAFGHLLQLFDAKDYDENMKGWRMEKFPFIPEVFKYKIKQDS
LNRNIIDKGAEKQIKIIKGLIDREDVDGIISATDFDREGQVIADEIFLYFSEKKPVYRLLLNEWTPDEVKKGMEKLKPNE
EMQSLQDAGIGRQWADWIIGINLTSVTTIKYKLNDGQILNIGRVLLPTLKIIYDRDKEIENFKSSAYYKLNGTFKTSKNE
EFEGTYYENNSEKFEDKKVLDEIKEKIKDKNAEIIEKQTERKKEYPPFLFNLSNLQGYITSKYKGWTSDKVLKVAQSLYE
KKFITYPRTGSMALEESLVDRTRNVLEKLKVGLPYEEQIKFVQNKRVFDNSKVESHSAIVPTYMKPNNLTQDERIVYTAV
KNRFLMQFMPVAEFEETKITSKINDKEVKGVFISKGKVQLIEGWRVVEKIDSKDTILPMVNEKEIVDLVDSKINKVTKKP
PKKHTEKTLLRVMETCGKSFKEDAEEDSEEMMASILSGFSIGTPATRAETIKKLKDVGYITTKGKSLTCTELGRTIVEIF
PVKELLDLEYTGRLEKTLSDIERGKVKKEDFLNLIKNFTIEGVKAIKNDDSMAKHFKVEAPEGVEVLGKCPNCGNPVIEG
ERGFGCVNWKNGCKYTIWKNDKYIASFGKQVTPQMVKLLLANGKVGFRNLKSKKGNTFAAYFIYEKDEEKGYFTWKMEFI

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=738, Percent_Identity=24.6612466124661, Blast_Score=118, Evalue=2e-26,
Organism=Escherichia coli, GI1788061, Length=617, Percent_Identity=28.8492706645057, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI1787529, Length=591, Percent_Identity=23.1810490693739, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32563869, Length=643, Percent_Identity=24.1057542768274, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17555378, Length=634, Percent_Identity=23.0283911671924, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323263, Length=678, Percent_Identity=24.4837758112094, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 83190; Mature: 83190

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVIIAEKPSVARNIADAFNIKKRKDGYFEGEEYLITWAFGHLLQLFDAKDYDENMKGW
CCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCC
RMEKFPFIPEVFKYKIKQDSLNRNIIDKGAEKQIKIIKGLIDREDVDGIISATDFDREGQ
CCCCCCCCHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
VIADEIFLYFSEKKPVYRLLLNEWTPDEVKKGMEKLKPNEEMQSLQDAGIGRQWADWIIG
EEEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHEEEE
INLTSVTTIKYKLNDGQILNIGRVLLPTLKIIYDRDKEIENFKSSAYYKLNGTFKTSKNE
EEEEEEEEEEEEECCCCEEEHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECEEECCCCC
EFEGTYYENNSEKFEDKKVLDEIKEKIKDKNAEIIEKQTERKKEYPPFLFNLSNLQGYIT
CCCCCEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEE
SKYKGWTSDKVLKVAQSLYEKKFITYPRTGSMALEESLVDRTRNVLEKLKVGLPYEEQIK
ECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FVQNKRVFDNSKVESHSAIVPTYMKPNNLTQDERIVYTAVKNRFLMQFMPVAEFEETKIT
HHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHEEHHHHHHHHHHHHCCCCHHHHHHHH
SKINDKEVKGVFISKGKVQLIEGWRVVEKIDSKDTILPMVNEKEIVDLVDSKINKVTKKP
HCCCCCCCCEEEEECCCEEEEHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHCCC
PKKHTEKTLLRVMETCGKSFKEDAEEDSEEMMASILSGFSIGTPATRAETIKKLKDVGYI
CHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEE
TTKGKSLTCTELGRTIVEIFPVKELLDLEYTGRLEKTLSDIERGKVKKEDFLNLIKNFTI
EECCCEEEHHHHHHHHHHHHCHHHHHCCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCH
EGVKAIKNDDSMAKHFKVEAPEGVEVLGKCPNCGNPVIEGERGFGCVNWKNGCKYTIWKN
HHHHHHCCCCHHHHHEECCCCCCHHHHCCCCCCCCCCEECCCCCEEEECCCCCEEEEECC
DKYIASFGKQVTPQMVKLLLANGKVGFRNLKSKKGNTFAAYFIYEKDEEKGYFTWKMEFI
CHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEECCCCCEEEEEEECC
>Mature Secondary Structure
MKKVIIAEKPSVARNIADAFNIKKRKDGYFEGEEYLITWAFGHLLQLFDAKDYDENMKGW
CCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCC
RMEKFPFIPEVFKYKIKQDSLNRNIIDKGAEKQIKIIKGLIDREDVDGIISATDFDREGQ
CCCCCCCCHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
VIADEIFLYFSEKKPVYRLLLNEWTPDEVKKGMEKLKPNEEMQSLQDAGIGRQWADWIIG
EEEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHEEEE
INLTSVTTIKYKLNDGQILNIGRVLLPTLKIIYDRDKEIENFKSSAYYKLNGTFKTSKNE
EEEEEEEEEEEEECCCCEEEHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECEEECCCCC
EFEGTYYENNSEKFEDKKVLDEIKEKIKDKNAEIIEKQTERKKEYPPFLFNLSNLQGYIT
CCCCCEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEE
SKYKGWTSDKVLKVAQSLYEKKFITYPRTGSMALEESLVDRTRNVLEKLKVGLPYEEQIK
ECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FVQNKRVFDNSKVESHSAIVPTYMKPNNLTQDERIVYTAVKNRFLMQFMPVAEFEETKIT
HHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHEEHHHHHHHHHHHHCCCCHHHHHHHH
SKINDKEVKGVFISKGKVQLIEGWRVVEKIDSKDTILPMVNEKEIVDLVDSKINKVTKKP
HCCCCCCCCEEEEECCCEEEEHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHCCC
PKKHTEKTLLRVMETCGKSFKEDAEEDSEEMMASILSGFSIGTPATRAETIKKLKDVGYI
CHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEE
TTKGKSLTCTELGRTIVEIFPVKELLDLEYTGRLEKTLSDIERGKVKKEDFLNLIKNFTI
EECCCEEEHHHHHHHHHHHHCHHHHHCCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCH
EGVKAIKNDDSMAKHFKVEAPEGVEVLGKCPNCGNPVIEGERGFGCVNWKNGCKYTIWKN
HHHHHHCCCCHHHHHEECCCCCCHHHHCCCCCCCCCCEECCCCCEEEECCCCCEEEEECC
DKYIASFGKQVTPQMVKLLLANGKVGFRNLKSKKGNTFAAYFIYEKDEEKGYFTWKMEFI
CHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA