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The map label for this gene is puuR [H]

Identifier: 18310949

GI number: 18310949

Start: 2256585

End: 2257124

Strand: Direct

Name: puuR [H]

Synonym: CPE1967

Alternate gene names: 18310949

Gene position: 2256585-2257124 (Clockwise)

Preceding gene: 18310946

Following gene: 18310950

Centisome position: 74.44

GC content: 32.96

Gene sequence:

>540_bases
TTGGAAATAGGAGATAAAATTAGACGTCTCAGAGTGGCCAAACAGTTGACTCAAGAAGAATTAGCAAATAGATGTGAATT
ATCTAAGGGATTTATTTCTCAGCTTGAGAATGACCTTACTTCTCCATCTATAGCTACATTAATAGATATATTAGATATAT
TAGGAACTAATTTAACGGAATTCTTTAGTGAAGATACTAATGAAAAGATAGCCTTCTCTAAGGATGATATGTTTGAAACT
GAAAATGAAGAATTAAAATATAACCTTATGTGGTTAGTTCCAAATGCTCAAAAGAATGATATGGAACCTATAATGATAAC
GCTTGAACCTGGAGGACAATATATTGAAGAAGAGCCTCATGAGGGTGAGGAATTTGGGTATGTCCTATCTGGAGCAATAT
ATCTTCATCTAGGAGACAAAAAAGTAAAAGTAAGAAAAGGCGAGAGTTTCTACTTTAAGCCCAAGGCTAATCATTATATT
TCTAATGCAGGAAAAATTTCTGCAAAGGTTATATGGATAAGTACTCCACCATCATTTTAG

Upstream 100 bases:

>100_bases
GATTTTTATTTTAAAAGTATTTATAATTATTTGTGTTTGTTTAGTAATTTTAAACTTAAAGTTTAGTAAAACTTATAGTA
ATGAAATTGAGGTGTAAAAC

Downstream 100 bases:

>100_bases
TATAAACATTTAGAAAGCAGAGGTGTTAGATTTGAAAGATAATAATATTATTGAATTAAAAGGAATAACTAAATCATATG
GAAAAGATACAATTTTAGAT

Product: DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MEIGDKIRRLRVAKQLTQEELANRCELSKGFISQLENDLTSPSIATLIDILDILGTNLTEFFSEDTNEKIAFSKDDMFET
ENEELKYNLMWLVPNAQKNDMEPIMITLEPGGQYIEEEPHEGEEFGYVLSGAIYLHLGDKKVKVRKGESFYFKPKANHYI
SNAGKISAKVIWISTPPSF

Sequences:

>Translated_179_residues
MEIGDKIRRLRVAKQLTQEELANRCELSKGFISQLENDLTSPSIATLIDILDILGTNLTEFFSEDTNEKIAFSKDDMFET
ENEELKYNLMWLVPNAQKNDMEPIMITLEPGGQYIEEEPHEGEEFGYVLSGAIYLHLGDKKVKVRKGESFYFKPKANHYI
SNAGKISAKVIWISTPPSF
>Mature_179_residues
MEIGDKIRRLRVAKQLTQEELANRCELSKGFISQLENDLTSPSIATLIDILDILGTNLTEFFSEDTNEKIAFSKDDMFET
ENEELKYNLMWLVPNAQKNDMEPIMITLEPGGQYIEEEPHEGEEFGYVLSGAIYLHLGDKKVKVRKGESFYFKPKANHYI
SNAGKISAKVIWISTPPSF

Specific function: Represses puuD [H]

COG id: COG1396

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=180, Percent_Identity=30.5555555555556, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 20425; Mature: 20425

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIGDKIRRLRVAKQLTQEELANRCELSKGFISQLENDLTSPSIATLIDILDILGTNLTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
FFSEDTNEKIAFSKDDMFETENEELKYNLMWLVPNAQKNDMEPIMITLEPGGQYIEEEPH
HHCCCCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCC
EGEEFGYVLSGAIYLHLGDKKVKVRKGESFYFKPKANHYISNAGKISAKVIWISTPPSF
CCCCCCEEEEEEEEEEECCCEEEEEECCEEEECCCCCCEECCCCCEEEEEEEEECCCCC
>Mature Secondary Structure
MEIGDKIRRLRVAKQLTQEELANRCELSKGFISQLENDLTSPSIATLIDILDILGTNLTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
FFSEDTNEKIAFSKDDMFETENEELKYNLMWLVPNAQKNDMEPIMITLEPGGQYIEEEPH
HHCCCCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCC
EGEEFGYVLSGAIYLHLGDKKVKVRKGESFYFKPKANHYISNAGKISAKVIWISTPPSF
CCCCCCEEEEEEEEEEECCCEEEEEECCEEEECCCCCCEECCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]