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The map label for this gene is folC [H]

Identifier: 18310900

GI number: 18310900

Start: 2196988

End: 2198310

Strand: Reverse

Name: folC [H]

Synonym: CPE1918

Alternate gene names: 18310900

Gene position: 2198310-2196988 (Counterclockwise)

Preceding gene: 18310901

Following gene: 18310899

Centisome position: 72.52

GC content: 29.33

Gene sequence:

>1323_bases
ATGAATTATGATGAAGCTATGGAGTATATTGTTACTGCATCAAGGTTTGGAATGAATTTAGGATTAGATAGAATAGAAAA
GGTTTTGGAGTTTTTAGGGAATCCTCATAAGGACACTAAGTTTATACATATTGGGGGAACAAATGGCAAGGGGTCAACTA
CTGCCATGATAAGTTCAGTTTTAAGGGAAGCTGGTTTTAAGGTTGGAATGTATACCTCTCCTTATTTAGAGGAATTTGAA
GAGAGAATACAGATTAATGGGGAGAATATAAATAAAGATGATTTAGCAATTCTTATGGGAGATGTTAAAAAGGCTATAGA
TAAAGTTATGGAGCTTGGCTATGAAAGTCCAACTCAGTTTGAAATAATAACTGCTTTAATGTTTTATTATTTTTCTAAAA
AGAAAGTTGATTATGCTGTCTTAGAAGTTGGTTTAGGAGGAAGATTAGATGCAACTAATGTGGTCCTTCCTGAGGTAGTT
GTACTTACTTCAATAAGCCTAGATCATATGAATATTTTAGGAAATACCATAGAGGAAATTGCAAAGGAAAAGTGTGGAAT
AATAAAGAGTGGTGTGCCTGTAATATCTTATCCACAAGAAGAAAAGGCCTTAGAAGTAATAAAAGAGAGATGTAATGAAT
TAGGTTGTAATTTAGAGGTTGTTAATACTGAAGACATAAAAAACATAATTATAGATAAAGAAAAACATGTTCAGAAAATA
GAAGTTTCACTAGAAGATGAAATTTTAGAAATAGATTTAGGTTTACTTGGAGATCATCAAGTAAAGAATTTCTTAGTGGC
ATTAAAAACCTTAATTAAATTAAGAAATAATGGAGTAAACATAAGCAATGAAGCTATTAAGCAAGGGTTTAAAAAAGTAA
GATGGATTGGAAGAATGGAAGTATTAAATGAAAATCCCTTAGTTGTTATAGATGGAGCTCACAATATTGATGGAATAAGA
AATTTGAGAAATAGTATAGATAAATATTTTAATTTTAAAAATATAACATTAATATTAGGGGTTCTAGGTGATAAACAAGT
AGATGAAATGGTAGAAGAAATAACTAGAGGAGTAGATAAGGTAGTTATAGTTGAACCTCATAGTGATAGGGCAGAGGACA
TTGAAGATTTATATATTAAAGTTGAAAAATATGTAAAAGAAGTTTTTAAATTTAAGGATTATAAAGAGGCCTATAATAAG
GCATATTCTTTTACTAATGATGATGATTTATTATTAATTTGTGGTTCTTTATATATGGTTGGAGATATGAGAAAGGCCAT
AAGAAATAATGATAATACATCCTTAAGAAGTATAAAGTATTAG

Upstream 100 bases:

>100_bases
TAGAATCCTATTTATAGTTTTCAAGATCTGACTCGTAGAACAGGGAATTAAACAAGATTTATAGATATTTATAGATTTTT
GGCAACGGGCTATAGGAATG

Downstream 100 bases:

>100_bases
ACTTAAGTAGATAAATTGTATTTAACTGGATAAATGAGAAAATTAAAATTTGAACTATTGTTAACAAAAGGTATATAATT
TATTCATAAAATATTATGGA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MNYDEAMEYIVTASRFGMNLGLDRIEKVLEFLGNPHKDTKFIHIGGTNGKGSTTAMISSVLREAGFKVGMYTSPYLEEFE
ERIQINGENINKDDLAILMGDVKKAIDKVMELGYESPTQFEIITALMFYYFSKKKVDYAVLEVGLGGRLDATNVVLPEVV
VLTSISLDHMNILGNTIEEIAKEKCGIIKSGVPVISYPQEEKALEVIKERCNELGCNLEVVNTEDIKNIIIDKEKHVQKI
EVSLEDEILEIDLGLLGDHQVKNFLVALKTLIKLRNNGVNISNEAIKQGFKKVRWIGRMEVLNENPLVVIDGAHNIDGIR
NLRNSIDKYFNFKNITLILGVLGDKQVDEMVEEITRGVDKVVIVEPHSDRAEDIEDLYIKVEKYVKEVFKFKDYKEAYNK
AYSFTNDDDLLLICGSLYMVGDMRKAIRNNDNTSLRSIKY

Sequences:

>Translated_440_residues
MNYDEAMEYIVTASRFGMNLGLDRIEKVLEFLGNPHKDTKFIHIGGTNGKGSTTAMISSVLREAGFKVGMYTSPYLEEFE
ERIQINGENINKDDLAILMGDVKKAIDKVMELGYESPTQFEIITALMFYYFSKKKVDYAVLEVGLGGRLDATNVVLPEVV
VLTSISLDHMNILGNTIEEIAKEKCGIIKSGVPVISYPQEEKALEVIKERCNELGCNLEVVNTEDIKNIIIDKEKHVQKI
EVSLEDEILEIDLGLLGDHQVKNFLVALKTLIKLRNNGVNISNEAIKQGFKKVRWIGRMEVLNENPLVVIDGAHNIDGIR
NLRNSIDKYFNFKNITLILGVLGDKQVDEMVEEITRGVDKVVIVEPHSDRAEDIEDLYIKVEKYVKEVFKFKDYKEAYNK
AYSFTNDDDLLLICGSLYMVGDMRKAIRNNDNTSLRSIKY
>Mature_440_residues
MNYDEAMEYIVTASRFGMNLGLDRIEKVLEFLGNPHKDTKFIHIGGTNGKGSTTAMISSVLREAGFKVGMYTSPYLEEFE
ERIQINGENINKDDLAILMGDVKKAIDKVMELGYESPTQFEIITALMFYYFSKKKVDYAVLEVGLGGRLDATNVVLPEVV
VLTSISLDHMNILGNTIEEIAKEKCGIIKSGVPVISYPQEEKALEVIKERCNELGCNLEVVNTEDIKNIIIDKEKHVQKI
EVSLEDEILEIDLGLLGDHQVKNFLVALKTLIKLRNNGVNISNEAIKQGFKKVRWIGRMEVLNENPLVVIDGAHNIDGIR
NLRNSIDKYFNFKNITLILGVLGDKQVDEMVEEITRGVDKVVIVEPHSDRAEDIEDLYIKVEKYVKEVFKFKDYKEAYNK
AYSFTNDDDLLLICGSLYMVGDMRKAIRNNDNTSLRSIKY

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=316, Percent_Identity=30.6962025316456, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI66932990, Length=316, Percent_Identity=30.6962025316456, Blast_Score=158, Evalue=9e-39,
Organism=Escherichia coli, GI1788654, Length=410, Percent_Identity=28.5365853658537, Blast_Score=162, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17553150, Length=468, Percent_Identity=28.4188034188034, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17553148, Length=468, Percent_Identity=28.4188034188034, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71984923, Length=468, Percent_Identity=28.4188034188034, Blast_Score=142, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6323760, Length=434, Percent_Identity=32.0276497695853, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6324815, Length=304, Percent_Identity=32.8947368421053, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322718, Length=435, Percent_Identity=22.9885057471264, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24641571, Length=336, Percent_Identity=30.0595238095238, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24581568, Length=206, Percent_Identity=30.5825242718447, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 49883; Mature: 49883

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYDEAMEYIVTASRFGMNLGLDRIEKVLEFLGNPHKDTKFIHIGGTNGKGSTTAMISSV
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHH
LREAGFKVGMYTSPYLEEFEERIQINGENINKDDLAILMGDVKKAIDKVMELGYESPTQF
HHHCCCEECCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EIITALMFYYFSKKKVDYAVLEVGLGGRLDATNVVLPEVVVLTSISLDHMNILGNTIEEI
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
AKEKCGIIKSGVPVISYPQEEKALEVIKERCNELGCNLEVVNTEDIKNIIIDKEKHVQKI
HHHHCCCHHCCCCCEECCCHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCHHHHHHH
EVSLEDEILEIDLGLLGDHQVKNFLVALKTLIKLRNNGVNISNEAIKQGFKKVRWIGRME
HHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VLNENPLVVIDGAHNIDGIRNLRNSIDKYFNFKNITLILGVLGDKQVDEMVEEITRGVDK
EECCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCE
VVIVEPHSDRAEDIEDLYIKVEKYVKEVFKFKDYKEAYNKAYSFTNDDDLLLICGSLYMV
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHH
GDMRKAIRNNDNTSLRSIKY
HHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNYDEAMEYIVTASRFGMNLGLDRIEKVLEFLGNPHKDTKFIHIGGTNGKGSTTAMISSV
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHH
LREAGFKVGMYTSPYLEEFEERIQINGENINKDDLAILMGDVKKAIDKVMELGYESPTQF
HHHCCCEECCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EIITALMFYYFSKKKVDYAVLEVGLGGRLDATNVVLPEVVVLTSISLDHMNILGNTIEEI
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
AKEKCGIIKSGVPVISYPQEEKALEVIKERCNELGCNLEVVNTEDIKNIIIDKEKHVQKI
HHHHCCCHHCCCCCEECCCHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCHHHHHHH
EVSLEDEILEIDLGLLGDHQVKNFLVALKTLIKLRNNGVNISNEAIKQGFKKVRWIGRME
HHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VLNENPLVVIDGAHNIDGIRNLRNSIDKYFNFKNITLILGVLGDKQVDEMVEEITRGVDK
EECCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCE
VVIVEPHSDRAEDIEDLYIKVEKYVKEVFKFKDYKEAYNKAYSFTNDDDLLLICGSLYMV
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHH
GDMRKAIRNNDNTSLRSIKY
HHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]