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The map label for this gene is pheS

Identifier: 18310868

GI number: 18310868

Start: 2167617

End: 2168642

Strand: Reverse

Name: pheS

Synonym: CPE1886

Alternate gene names: 18310868

Gene position: 2168642-2167617 (Counterclockwise)

Preceding gene: 18310869

Following gene: 18310867

Centisome position: 71.54

GC content: 32.16

Gene sequence:

>1026_bases
ATGAAAATGCAAGATAAGTTAAATCAAATTAAAGAATTAGCTTTAGTAGAAATTAAAGAAGCTAAAGATAGTACTACTAT
TGATACAATAAGAGTTAAATATCTTGGTAAAAAGGGAGAACTTACAACTATATTAAGAGGAATGGGATCTCTATCTAAAG
AGGAGAGACCAATAGTTGGTAAGTTAGCTAATGAGGTAAGAGAGGTTTTAGAAGCTGAATTAGAGGCTATAACAAAGGCT
GTTAAAGAAGCTGAAAAACAAGAAAAGCTTAAAAATGAAGTAATAGATATTTCAATGCCTGGTAAAAAACAAACAATAGG
AAAGAAACATCCATTAGAGCAAACTTTAGATGAAATGAAAAAAATATTTGTTTCAATGGGATTTGCTATAGAAGATGGTC
CAGAGGTTGAGAAAGATTATTATAACTTTGAAGCCTTAAACATTCCTAAGAATCATCCAGCTAGAAGTGAGCAAGATACA
TTCTACATAAATGATAATGTAGTTTTAAGAACTCAAACTTCTCCAGTTCAAGCTAGAGTAATGGAAAAACAACAACCACC
AATAAAAATGATATCACCTGGTAAGGTATTTAGATCAGATGCTGTTGATGCTACACATTCACCAATATTCTACCAAATGG
AAGGTCTTGTTATAGATAAAGATATAACTTTTGCAGATCTTAAGGGAACTTTAGAATTATTTGCTAAGAAAATGTTTGGT
GATAAAGTAAAAACTAAGTTTAGACCACATCATTTCCCATTCACTGAGCCATCAGCTGAAATGGATGCTACATGCTTTGT
ATGTAACGGAAAAGGATGTAAAGTATGTAAGGGTGAAGGTTGGATAGAAATACTAGGTTGTGGTATGGTTCACCCTCAAG
TTTTAAGAAACTGTGGAATAGACCCAGAAGTTTATAGTGGATTTGCTTTCGGCTTTGGTGTAGACAGAATGGTTATGCTT
AAGTATGGAATAGATGACATAAGATTATTATACGAAAGTGATATGAGATTCTTAAATCAATTCTAG

Upstream 100 bases:

>100_bases
ATTAAATCTAAGATTAAATAATGACTAAAAAAATAGATATTAGGGTTCTAAGAGCTTTAAGATAAAATCTAATATATTAA
TTATATATCAGGAAAGGAGA

Downstream 100 bases:

>100_bases
TTTTAAAATAGGAGGTAAAAAAGATGAAAGTACCATTTAATTGGCTTAAAGATTACGTTGATATAGATATGACAGCAGAA
GAGTTAGGCGATGCTTTAAC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKMQDKLNQIKELALVEIKEAKDSTTIDTIRVKYLGKKGELTTILRGMGSLSKEERPIVGKLANEVREVLEAELEAITKA
VKEAEKQEKLKNEVIDISMPGKKQTIGKKHPLEQTLDEMKKIFVSMGFAIEDGPEVEKDYYNFEALNIPKNHPARSEQDT
FYINDNVVLRTQTSPVQARVMEKQQPPIKMISPGKVFRSDAVDATHSPIFYQMEGLVIDKDITFADLKGTLELFAKKMFG
DKVKTKFRPHHFPFTEPSAEMDATCFVCNGKGCKVCKGEGWIEILGCGMVHPQVLRNCGIDPEVYSGFAFGFGVDRMVML
KYGIDDIRLLYESDMRFLNQF

Sequences:

>Translated_341_residues
MKMQDKLNQIKELALVEIKEAKDSTTIDTIRVKYLGKKGELTTILRGMGSLSKEERPIVGKLANEVREVLEAELEAITKA
VKEAEKQEKLKNEVIDISMPGKKQTIGKKHPLEQTLDEMKKIFVSMGFAIEDGPEVEKDYYNFEALNIPKNHPARSEQDT
FYINDNVVLRTQTSPVQARVMEKQQPPIKMISPGKVFRSDAVDATHSPIFYQMEGLVIDKDITFADLKGTLELFAKKMFG
DKVKTKFRPHHFPFTEPSAEMDATCFVCNGKGCKVCKGEGWIEILGCGMVHPQVLRNCGIDPEVYSGFAFGFGVDRMVML
KYGIDDIRLLYESDMRFLNQF
>Mature_341_residues
MKMQDKLNQIKELALVEIKEAKDSTTIDTIRVKYLGKKGELTTILRGMGSLSKEERPIVGKLANEVREVLEAELEAITKA
VKEAEKQEKLKNEVIDISMPGKKQTIGKKHPLEQTLDEMKKIFVSMGFAIEDGPEVEKDYYNFEALNIPKNHPARSEQDT
FYINDNVVLRTQTSPVQARVMEKQQPPIKMISPGKVFRSDAVDATHSPIFYQMEGLVIDKDITFADLKGTLELFAKKMFG
DKVKTKFRPHHFPFTEPSAEMDATCFVCNGKGCKVCKGEGWIEILGCGMVHPQVLRNCGIDPEVYSGFAFGFGVDRMVML
KYGIDDIRLLYESDMRFLNQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=269, Percent_Identity=30.4832713754647, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI5729820, Length=285, Percent_Identity=29.4736842105263, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1788007, Length=335, Percent_Identity=48.3582089552239, Blast_Score=354, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI32563657, Length=271, Percent_Identity=31.3653136531365, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17508957, Length=271, Percent_Identity=31.3653136531365, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI32566635, Length=252, Percent_Identity=31.3492063492063, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321087, Length=285, Percent_Identity=30.8771929824561, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=279, Percent_Identity=27.9569892473118, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18858079, Length=287, Percent_Identity=31.0104529616725, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17137424, Length=249, Percent_Identity=27.710843373494, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_CLOPE (Q8XJ75)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_562802.1
- ProteinModelPortal:   Q8XJ75
- SMR:   Q8XJ75
- GeneID:   990196
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE1886
- NMPDR:   fig|195102.1.peg.1949
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q8XJ75
- ProtClustDB:   PRK00488
- BioCyc:   CPER195102:CPE1886-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38659; Mature: 38659

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMQDKLNQIKELALVEIKEAKDSTTIDTIRVKYLGKKGELTTILRGMGSLSKEERPIVG
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCHHH
KLANEVREVLEAELEAITKAVKEAEKQEKLKNEVIDISMPGKKQTIGKKHPLEQTLDEMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHH
KIFVSMGFAIEDGPEVEKDYYNFEALNIPKNHPARSEQDTFYINDNVVLRTQTSPVQARV
HHHHHHCCEECCCCCCCHHHCCEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCHHHHH
MEKQQPPIKMISPGKVFRSDAVDATHSPIFYQMEGLVIDKDITFADLKGTLELFAKKMFG
HHHCCCCEEEECCCHHHHHCCCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHC
DKVKTKFRPHHFPFTEPSAEMDATCFVCNGKGCKVCKGEGWIEILGCGMVHPQVLRNCGI
HHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCEEEEEECCCCCHHHHHHCCC
DPEVYSGFAFGFGVDRMVMLKYGIDDIRLLYESDMRFLNQF
CHHHHCCCHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKMQDKLNQIKELALVEIKEAKDSTTIDTIRVKYLGKKGELTTILRGMGSLSKEERPIVG
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCHHH
KLANEVREVLEAELEAITKAVKEAEKQEKLKNEVIDISMPGKKQTIGKKHPLEQTLDEMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHH
KIFVSMGFAIEDGPEVEKDYYNFEALNIPKNHPARSEQDTFYINDNVVLRTQTSPVQARV
HHHHHHCCEECCCCCCCHHHCCEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCHHHHH
MEKQQPPIKMISPGKVFRSDAVDATHSPIFYQMEGLVIDKDITFADLKGTLELFAKKMFG
HHHCCCCEEEECCCHHHHHCCCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHC
DKVKTKFRPHHFPFTEPSAEMDATCFVCNGKGCKVCKGEGWIEILGCGMVHPQVLRNCGI
HHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCEEEEEECCCCCHHHHHHCCC
DPEVYSGFAFGFGVDRMVMLKYGIDDIRLLYESDMRFLNQF
CHHHHCCCHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842