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The map label for this gene is pheT

Identifier: 18310867

GI number: 18310867

Start: 2165212

End: 2167593

Strand: Reverse

Name: pheT

Synonym: CPE1885

Alternate gene names: 18310867

Gene position: 2167593-2165212 (Counterclockwise)

Preceding gene: 18310868

Following gene: 18310866

Centisome position: 71.5

GC content: 31.15

Gene sequence:

>2382_bases
ATGAAAGTACCATTTAATTGGCTTAAAGATTACGTTGATATAGATATGACAGCAGAAGAGTTAGGCGATGCTTTAACTTT
ATCAGGATCAAAAGTAGAAGAGGTCGTAACTGCTGGTGCAGAAATAGAAAATATAGTTACTTGTCAAATAGATAAAATAG
AACAACATCCTGATGCTGAAAAGCTTAAAATTTGTCAAGTAAACATAGGATCAGAAATGATTCAAATAGTAACAGCTGCA
GATAATATGAAAGAACATGATAAAGTTCCAGTAGCTTTACATGGTGCAATATTAGCAGATGGAAGCAAAATCAAAAAAGG
TAAATTAAGAGGAGAAGTTTCAAACGGAATGTTCTGTTCAGAAGAAGAATTAGGAATTGCTGACGGAAAAGAAGTTATTG
GACTTATGATTTTACCAGAGGATGCTCCAATTGGTGTTTGTATAAAAGAATATTTAGGTCTTAATAAACAAGTTTTAGAT
TTTGAAATAACTTCAAATAGACCTGATTGTTTAAGTGTTTTAGGTATGGCTAGAGAGACAGCAGCCACAACAAGAAAAAC
TTACAGAATGCCAAATACTAAGTTTGAAGTTAAATGTGATAAAAACATAAATGATGAAATAAAAGTTACAGTTAATGATA
AGCTTTGTAATAGATATATGGCTAGAGGAGTTAAAAATGTAAAGGTAGCTCCATCACCAACTTGGATGCAAGAAAGATTA
TTAGATGCTGGGGTTAGACCTATAAATAACATAGTTGATATTACTAACTTTGTTATGTTAGAGCTTGGTCAACCAATGCA
TGCCTTTGATAGAAGAGAATTAAAATCTTCTAACATAGTAGTTGAGAGAGCTAAAGATGGAGATAAATTCGTAACTTTAG
ATGAAATAGAAAGAGAATTAAATTCAGATGTTCTTTGCATAAAAGATGGAGAAGAGATAGTTGCTTTAGCTGGTATAATG
GGTGGATTAAACTCCGGTGTTAAAGATGACACAACAGAGATTGTATTTGAATCAGCTAACTTTGATGGAACTAATATAAG
AGTTAATTCTAAAAATTTAGGCTTAAGAACTGAAAGTTCATCAAGATTTGAAAAAGACATAGATCCAAACTTAGCAGCTT
TAGCTTTAGACAGAGCATGTGCTTTAGTTGAAGAACTAGGCTGTGGAGAAATTATGGAAGGCACTATAGATGTCTATAAT
AATGTTAAAGAAGAAAAAACTCTAGAAGTAAGTGTTAGCTGGATAAACAAATTCTTAGGAACTTCATTAGAAGCAGAAGA
GATGAAGAAATGTTTAGATAGCTTAGATTTAAAAACTACTATAAATGGAGATACATTAGAAATATTAGTACCTACATTTA
GAATAGATGTTGATATAAGAGAAGATGTAGCAGAAGAAGTTGCAAGAATCTATGGATACAATAATATTCCAGCTACTTTA
ATATCAACTAGTACAGAAAAAGAAGCTAAGGACAAGAAACAATTATTAGATGAAAAAGTTATAGATACAATGTTAGCATC
AGGCTTAAATCAATCTATAAGTTACTCATTTGTAAGTCCAAAGGTATTTGATAAAATCTGTGTACCAGAAGATAGTAGCC
TTAGAAATGTTGTTAAAATAAAGAATCCACTAGGAGAAGATTACAGTGTTATGAGAACTTCAACACTTCCTTCAATGATG
GAATCTTTAGCTAGAAACTATTCAAGAAGCAATGAGATAGTTAGATTATTTGAAATAGGCAAAACTTATATTCCAAATTC
AGATGAAAATGAATTACCAACTGAAAGAAATATATTAACAATTGGTATTTATGGACAATGTGATTACTTTGATTTAAAAG
GAATAGTTGAAAACTTATTAGATTCATTAGGTCTTAAGAGTGCATCATTCAAGAGAATGAGTGAAAACCCAAGTTATCAC
CCAGGAAAAACTGCTGAGTTAATATTAGGTAGAACTTCACTAGGTGTTTTAGGAGAAATTCACCCAGATGTTAATGAAAA
TTACGGAGTAGATGTTCCTTGCTATGTTGCTGAATTAGACTTAGATGCTATGTATAACTTCTCAAAAACAGAGAAATATT
ACAAACCATTACCTAAGTATCCAGCAATAACAAGAGATATAGCTTTACTTGTTGATGATGCAGTTTTAGTTCAAGAAATA
GAAGATGCAATAAGAAAAGCTGGTGGTCAAGTAGTTGAAAAAGTTGAATTATTTGACGTTTATAAAGGTGCTCAAATACC
AGAAGGTAAGAAGAGTATTGCATATGCTATCGCTTACAGAGATCCAAGCAAAACTTTAAAAGATAAAGATATAAATAAAG
TTCATGATAAGATACTAAGAGCTTTAGAATATAAGCTAGGAGCTCAATTAAGAGAGCAATAA

Upstream 100 bases:

>100_bases
TGGTTATGCTTAAGTATGGAATAGATGACATAAGATTATTATACGAAAGTGATATGAGATTCTTAAATCAATTCTAGTTT
TAAAATAGGAGGTAAAAAAG

Downstream 100 bases:

>100_bases
TAGTTTTAAATATGTTATGTATATGTTAAAATGTACATAACATATTTTTTATTATGGAAGTAAGAGGTGTTTAAGAAAGT
ATGGCGACTGTTACGGTAAC

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MKVPFNWLKDYVDIDMTAEELGDALTLSGSKVEEVVTAGAEIENIVTCQIDKIEQHPDAEKLKICQVNIGSEMIQIVTAA
DNMKEHDKVPVALHGAILADGSKIKKGKLRGEVSNGMFCSEEELGIADGKEVIGLMILPEDAPIGVCIKEYLGLNKQVLD
FEITSNRPDCLSVLGMARETAATTRKTYRMPNTKFEVKCDKNINDEIKVTVNDKLCNRYMARGVKNVKVAPSPTWMQERL
LDAGVRPINNIVDITNFVMLELGQPMHAFDRRELKSSNIVVERAKDGDKFVTLDEIERELNSDVLCIKDGEEIVALAGIM
GGLNSGVKDDTTEIVFESANFDGTNIRVNSKNLGLRTESSSRFEKDIDPNLAALALDRACALVEELGCGEIMEGTIDVYN
NVKEEKTLEVSVSWINKFLGTSLEAEEMKKCLDSLDLKTTINGDTLEILVPTFRIDVDIREDVAEEVARIYGYNNIPATL
ISTSTEKEAKDKKQLLDEKVIDTMLASGLNQSISYSFVSPKVFDKICVPEDSSLRNVVKIKNPLGEDYSVMRTSTLPSMM
ESLARNYSRSNEIVRLFEIGKTYIPNSDENELPTERNILTIGIYGQCDYFDLKGIVENLLDSLGLKSASFKRMSENPSYH
PGKTAELILGRTSLGVLGEIHPDVNENYGVDVPCYVAELDLDAMYNFSKTEKYYKPLPKYPAITRDIALLVDDAVLVQEI
EDAIRKAGGQVVEKVELFDVYKGAQIPEGKKSIAYAIAYRDPSKTLKDKDINKVHDKILRALEYKLGAQLREQ

Sequences:

>Translated_793_residues
MKVPFNWLKDYVDIDMTAEELGDALTLSGSKVEEVVTAGAEIENIVTCQIDKIEQHPDAEKLKICQVNIGSEMIQIVTAA
DNMKEHDKVPVALHGAILADGSKIKKGKLRGEVSNGMFCSEEELGIADGKEVIGLMILPEDAPIGVCIKEYLGLNKQVLD
FEITSNRPDCLSVLGMARETAATTRKTYRMPNTKFEVKCDKNINDEIKVTVNDKLCNRYMARGVKNVKVAPSPTWMQERL
LDAGVRPINNIVDITNFVMLELGQPMHAFDRRELKSSNIVVERAKDGDKFVTLDEIERELNSDVLCIKDGEEIVALAGIM
GGLNSGVKDDTTEIVFESANFDGTNIRVNSKNLGLRTESSSRFEKDIDPNLAALALDRACALVEELGCGEIMEGTIDVYN
NVKEEKTLEVSVSWINKFLGTSLEAEEMKKCLDSLDLKTTINGDTLEILVPTFRIDVDIREDVAEEVARIYGYNNIPATL
ISTSTEKEAKDKKQLLDEKVIDTMLASGLNQSISYSFVSPKVFDKICVPEDSSLRNVVKIKNPLGEDYSVMRTSTLPSMM
ESLARNYSRSNEIVRLFEIGKTYIPNSDENELPTERNILTIGIYGQCDYFDLKGIVENLLDSLGLKSASFKRMSENPSYH
PGKTAELILGRTSLGVLGEIHPDVNENYGVDVPCYVAELDLDAMYNFSKTEKYYKPLPKYPAITRDIALLVDDAVLVQEI
EDAIRKAGGQVVEKVELFDVYKGAQIPEGKKSIAYAIAYRDPSKTLKDKDINKVHDKILRALEYKLGAQLREQ
>Mature_793_residues
MKVPFNWLKDYVDIDMTAEELGDALTLSGSKVEEVVTAGAEIENIVTCQIDKIEQHPDAEKLKICQVNIGSEMIQIVTAA
DNMKEHDKVPVALHGAILADGSKIKKGKLRGEVSNGMFCSEEELGIADGKEVIGLMILPEDAPIGVCIKEYLGLNKQVLD
FEITSNRPDCLSVLGMARETAATTRKTYRMPNTKFEVKCDKNINDEIKVTVNDKLCNRYMARGVKNVKVAPSPTWMQERL
LDAGVRPINNIVDITNFVMLELGQPMHAFDRRELKSSNIVVERAKDGDKFVTLDEIERELNSDVLCIKDGEEIVALAGIM
GGLNSGVKDDTTEIVFESANFDGTNIRVNSKNLGLRTESSSRFEKDIDPNLAALALDRACALVEELGCGEIMEGTIDVYN
NVKEEKTLEVSVSWINKFLGTSLEAEEMKKCLDSLDLKTTINGDTLEILVPTFRIDVDIREDVAEEVARIYGYNNIPATL
ISTSTEKEAKDKKQLLDEKVIDTMLASGLNQSISYSFVSPKVFDKICVPEDSSLRNVVKIKNPLGEDYSVMRTSTLPSMM
ESLARNYSRSNEIVRLFEIGKTYIPNSDENELPTERNILTIGIYGQCDYFDLKGIVENLLDSLGLKSASFKRMSENPSYH
PGKTAELILGRTSLGVLGEIHPDVNENYGVDVPCYVAELDLDAMYNFSKTEKYYKPLPKYPAITRDIALLVDDAVLVQEI
EDAIRKAGGQVVEKVELFDVYKGAQIPEGKKSIAYAIAYRDPSKTLKDKDINKVHDKILRALEYKLGAQLREQ

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=359, Percent_Identity=27.5766016713092, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI1788006, Length=820, Percent_Identity=34.390243902439, Blast_Score=450, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17533403, Length=357, Percent_Identity=24.3697478991597, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21355211, Length=358, Percent_Identity=24.0223463687151, Blast_Score=101, Evalue=2e-21,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_CLOPE (Q8XJ76)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_562801.1
- ProteinModelPortal:   Q8XJ76
- SMR:   Q8XJ76
- GeneID:   990195
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE1885
- NMPDR:   fig|195102.1.peg.1948
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- PhylomeDB:   Q8XJ76
- ProtClustDB:   PRK00629
- BioCyc:   CPER195102:CPE1885-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 88259; Mature: 88259

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVPFNWLKDYVDIDMTAEELGDALTLSGSKVEEVVTAGAEIENIVTCQIDKIEQHPDAE
CCCCHHHHHHHHCCCCCHHHCCCEEEECCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCC
KLKICQVNIGSEMIQIVTAADNMKEHDKVPVALHGAILADGSKIKKGKLRGEVSNGMFCS
CEEEEEEECCHHHHHHHHHHCCCHHHCCCCEEEECEEEECCCCCCCCCCCEECCCCEEEC
EEELGIADGKEVIGLMILPEDAPIGVCIKEYLGLNKQVLDFEITSNRPDCLSVLGMARET
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHH
AATTRKTYRMPNTKFEVKCDKNINDEIKVTVNDKLCNRYMARGVKNVKVAPSPTWMQERL
HHHHHHHCCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHCCCCCEEECCCCHHHHHHH
LDAGVRPINNIVDITNFVMLELGQPMHAFDRRELKSSNIVVERAKDGDKFVTLDEIEREL
HHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHH
NSDVLCIKDGEEIVALAGIMGGLNSGVKDDTTEIVFESANFDGTNIRVNSKNLGLRTESS
CCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCEECCCC
SRFEKDIDPNLAALALDRACALVEELGCGEIMEGTIDVYNNVKEEKTLEVSVSWINKFLG
CHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHC
TSLEAEEMKKCLDSLDLKTTINGDTLEILVPTFRIDVDIREDVAEEVARIYGYNNIPATL
CCCCHHHHHHHHHHCCCEEEECCCEEEEEEEEEEEEEEHHHHHHHHHHHHHCCCCCCCEE
ISTSTEKEAKDKKQLLDEKVIDTMLASGLNQSISYSFVSPKVFDKICVPEDSSLRNVVKI
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCCCHHHHHHH
KNPLGEDYSVMRTSTLPSMMESLARNYSRSNEIVRLFEIGKTYIPNSDENELPTERNILT
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEE
IGIYGQCDYFDLKGIVENLLDSLGLKSASFKRMSENPSYHPGKTAELILGRTSLGVLGEI
EEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCHHCCC
HPDVNENYGVDVPCYVAELDLDAMYNFSKTEKYYKPLPKYPAITRDIALLVDDAVLVQEI
CCCCCCCCCCCCCEEEEEECHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
EDAIRKAGGQVVEKVELFDVYKGAQIPEGKKSIAYAIAYRDPSKTLKDKDINKVHDKILR
HHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHCCCCHHHHHHHHHHH
ALEYKLGAQLREQ
HHHHHHHHHHHCC
>Mature Secondary Structure
MKVPFNWLKDYVDIDMTAEELGDALTLSGSKVEEVVTAGAEIENIVTCQIDKIEQHPDAE
CCCCHHHHHHHHCCCCCHHHCCCEEEECCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCC
KLKICQVNIGSEMIQIVTAADNMKEHDKVPVALHGAILADGSKIKKGKLRGEVSNGMFCS
CEEEEEEECCHHHHHHHHHHCCCHHHCCCCEEEECEEEECCCCCCCCCCCEECCCCEEEC
EEELGIADGKEVIGLMILPEDAPIGVCIKEYLGLNKQVLDFEITSNRPDCLSVLGMARET
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHH
AATTRKTYRMPNTKFEVKCDKNINDEIKVTVNDKLCNRYMARGVKNVKVAPSPTWMQERL
HHHHHHHCCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHCCCCCEEECCCCHHHHHHH
LDAGVRPINNIVDITNFVMLELGQPMHAFDRRELKSSNIVVERAKDGDKFVTLDEIEREL
HHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHH
NSDVLCIKDGEEIVALAGIMGGLNSGVKDDTTEIVFESANFDGTNIRVNSKNLGLRTESS
CCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCEECCCC
SRFEKDIDPNLAALALDRACALVEELGCGEIMEGTIDVYNNVKEEKTLEVSVSWINKFLG
CHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHC
TSLEAEEMKKCLDSLDLKTTINGDTLEILVPTFRIDVDIREDVAEEVARIYGYNNIPATL
CCCCHHHHHHHHHHCCCEEEECCCEEEEEEEEEEEEEEHHHHHHHHHHHHHCCCCCCCEE
ISTSTEKEAKDKKQLLDEKVIDTMLASGLNQSISYSFVSPKVFDKICVPEDSSLRNVVKI
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCCCHHHHHHH
KNPLGEDYSVMRTSTLPSMMESLARNYSRSNEIVRLFEIGKTYIPNSDENELPTERNILT
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEE
IGIYGQCDYFDLKGIVENLLDSLGLKSASFKRMSENPSYHPGKTAELILGRTSLGVLGEI
EEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCHHCCC
HPDVNENYGVDVPCYVAELDLDAMYNFSKTEKYYKPLPKYPAITRDIALLVDDAVLVQEI
CCCCCCCCCCCCCEEEEEECHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
EDAIRKAGGQVVEKVELFDVYKGAQIPEGKKSIAYAIAYRDPSKTLKDKDINKVHDKILR
HHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHCCCCHHHHHHHHHHH
ALEYKLGAQLREQ
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842