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The map label for this gene is engD [H]

Identifier: 18310848

GI number: 18310848

Start: 2136866

End: 2137963

Strand: Reverse

Name: engD [H]

Synonym: CPE1866

Alternate gene names: 18310848

Gene position: 2137963-2136866 (Counterclockwise)

Preceding gene: 18310850

Following gene: 18310847

Centisome position: 70.53

GC content: 32.33

Gene sequence:

>1098_bases
ATGAAATTAGGAATGGTTGGATTACCAAATGTTGGTAAAAGTACATTATTCAATGCTATTACAAAAGCAGGAGCTGAATC
AGCAAACTATCCTTTCTGTACAATAGAACCAAACGTAGGTGTTGTAAGTGTACCAGATAAGAGATTAGATGTTTTAGAAA
AAATATATAATACAAAGAAAAAAGTATATACTGCAATAGAGTTTTATGATATAGCAGGATTAGTTAAAGGTGCTAGTAAA
GGTGAAGGATTAGGAAATAAATTCTTATCACACATAAGAGAAGTTGCAGCTATCGTTCATGTTGTAAGATGCTTTGACGA
TGAGAATGTTGTTCACGTAGAAGGTTCTGTAGATCCAATAAGAGATATAGAAACTATAAACTTAGAACTTATCTTTGCAG
ACTTAGATGTTCTTGAAAGAAGAATGGAAAAGACTATGAAGTTAGTAAGATCAGGTGATAAAACTGCTAAGTTTGAGTAT
GATGTAATGGAAAGATTAAAAGCTCACTTAGAAGCAAATAAACCAGCTAGAACTTTAGAAGCTACTGAAGATGAAGAAGC
TTTCGTAAAAAGCTTATTCTTAATAACTTCAAAACCAGTTTTATATGCTTGTAACATATCAGAAGATGATATGATGGAAG
GAAACTTAGATAATGAATATGTTCAAAAAGTTAGAGCATATGCTGAAACTGAGAATTCAGGAATCATGGTTGTTTGCGCT
AAACTTGAAGAGGAATTATCAGGATTAGATGAAGAAGAAAAAGCTGAAATGTTATCTGAGTATGGATTAGAAGAATCAGG
TCTTGATAAACTTATACAAGCAAGTTACAAATTATTAGGATTAATGAGTTACTTAACTGCAGGGGTACAAGAAGTTAGAG
CTTGGACAATAAAACAAGGAACTAAAGCTCCACAAGCAGCAGGTAAAATTCACTCTGATATAGAAAGAGGATTCATAAGA
GCTGAGGTTGTTTCTTATGATGATTTAGTAGAATGTGGTTCAGAAGCAGCTGCTAAAGAAAAAGGTGTTTACAGATTAGA
AGGTAAAGAATACGTAATGAAAGATGGAGATATAGTTAACTTCAGATTCAACGTATAA

Upstream 100 bases:

>100_bases
TGGATAATTAGGAAACATAATACAGTTGTTGAAATATATATTAAGCTAGAGTATAATCATTAAGTATGAAATTAAATATA
AGAAAGGGACGTGTACATAG

Downstream 100 bases:

>100_bases
TATAAAAGAAAAAAATTATCATCAAATTTAAACAGTATTTTTAGACAAAAAAAGAGCTATTTGAAGTTTTCTTCAGATAG
CTCTTTTTCTTTATGGCGGG

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKLGMVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKIYNTKKKVYTAIEFYDIAGLVKGASK
GEGLGNKFLSHIREVAAIVHVVRCFDDENVVHVEGSVDPIRDIETINLELIFADLDVLERRMEKTMKLVRSGDKTAKFEY
DVMERLKAHLEANKPARTLEATEDEEAFVKSLFLITSKPVLYACNISEDDMMEGNLDNEYVQKVRAYAETENSGIMVVCA
KLEEELSGLDEEEKAEMLSEYGLEESGLDKLIQASYKLLGLMSYLTAGVQEVRAWTIKQGTKAPQAAGKIHSDIERGFIR
AEVVSYDDLVECGSEAAAKEKGVYRLEGKEYVMKDGDIVNFRFNV

Sequences:

>Translated_365_residues
MKLGMVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKIYNTKKKVYTAIEFYDIAGLVKGASK
GEGLGNKFLSHIREVAAIVHVVRCFDDENVVHVEGSVDPIRDIETINLELIFADLDVLERRMEKTMKLVRSGDKTAKFEY
DVMERLKAHLEANKPARTLEATEDEEAFVKSLFLITSKPVLYACNISEDDMMEGNLDNEYVQKVRAYAETENSGIMVVCA
KLEEELSGLDEEEKAEMLSEYGLEESGLDKLIQASYKLLGLMSYLTAGVQEVRAWTIKQGTKAPQAAGKIHSDIERGFIR
AEVVSYDDLVECGSEAAAKEKGVYRLEGKEYVMKDGDIVNFRFNV
>Mature_365_residues
MKLGMVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKIYNTKKKVYTAIEFYDIAGLVKGASK
GEGLGNKFLSHIREVAAIVHVVRCFDDENVVHVEGSVDPIRDIETINLELIFADLDVLERRMEKTMKLVRSGDKTAKFEY
DVMERLKAHLEANKPARTLEATEDEEAFVKSLFLITSKPVLYACNISEDDMMEGNLDNEYVQKVRAYAETENSGIMVVCA
KLEEELSGLDEEEKAEMLSEYGLEESGLDKLIQASYKLLGLMSYLTAGVQEVRAWTIKQGTKAPQAAGKIHSDIERGFIR
AEVVSYDDLVECGSEAAAKEKGVYRLEGKEYVMKDGDIVNFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=371, Percent_Identity=46.900269541779, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI58761502, Length=222, Percent_Identity=44.5945945945946, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI24308117, Length=111, Percent_Identity=36.036036036036, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1787454, Length=365, Percent_Identity=53.972602739726, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1789574, Length=152, Percent_Identity=31.5789473684211, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17509631, Length=368, Percent_Identity=46.4673913043478, Blast_Score=330, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI17508313, Length=99, Percent_Identity=35.3535353535354, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319499, Length=371, Percent_Identity=42.3180592991914, Blast_Score=280, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6321773, Length=380, Percent_Identity=37.8947368421053, Blast_Score=239, Evalue=7e-64,
Organism=Saccharomyces cerevisiae, GI6321649, Length=351, Percent_Identity=27.6353276353276, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321962, Length=147, Percent_Identity=29.2517006802721, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640873, Length=369, Percent_Identity=44.9864498644986, Blast_Score=305, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24640877, Length=369, Percent_Identity=44.9864498644986, Blast_Score=305, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24640875, Length=369, Percent_Identity=44.9864498644986, Blast_Score=305, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24640879, Length=328, Percent_Identity=43.9024390243902, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24585318, Length=138, Percent_Identity=32.6086956521739, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI20129375, Length=121, Percent_Identity=30.5785123966942, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40625; Mature: 40625

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGMVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKIYNTKK
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHHHHH
KVYTAIEFYDIAGLVKGASKGEGLGNKFLSHIREVAAIVHVVRCFDDENVVHVEGSVDPI
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
RDIETINLELIFADLDVLERRMEKTMKLVRSGDKTAKFEYDVMERLKAHLEANKPARTLE
CCCEEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCHHCC
ATEDEEAFVKSLFLITSKPVLYACNISEDDMMEGNLDNEYVQKVRAYAETENSGIMVVCA
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHH
KLEEELSGLDEEEKAEMLSEYGLEESGLDKLIQASYKLLGLMSYLTAGVQEVRAWTIKQG
HHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TKAPQAAGKIHSDIERGFIRAEVVSYDDLVECGSEAAAKEKGVYRLEGKEYVMKDGDIVN
CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHCCCEEECCCEEEECCCCEEE
FRFNV
EEECC
>Mature Secondary Structure
MKLGMVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKIYNTKK
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHHHHH
KVYTAIEFYDIAGLVKGASKGEGLGNKFLSHIREVAAIVHVVRCFDDENVVHVEGSVDPI
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
RDIETINLELIFADLDVLERRMEKTMKLVRSGDKTAKFEYDVMERLKAHLEANKPARTLE
CCCEEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCHHCC
ATEDEEAFVKSLFLITSKPVLYACNISEDDMMEGNLDNEYVQKVRAYAETENSGIMVVCA
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHH
KLEEELSGLDEEEKAEMLSEYGLEESGLDKLIQASYKLLGLMSYLTAGVQEVRAWTIKQG
HHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TKAPQAAGKIHSDIERGFIRAEVVSYDDLVECGSEAAAKEKGVYRLEGKEYVMKDGDIVN
CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHCCCEEECCCEEEECCCCEEE
FRFNV
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]