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The map label for this gene is dapF

Identifier: 18310828

GI number: 18310828

Start: 2116533

End: 2117351

Strand: Reverse

Name: dapF

Synonym: CPE1846

Alternate gene names: 18310828

Gene position: 2117351-2116533 (Counterclockwise)

Preceding gene: 18310830

Following gene: 18310827

Centisome position: 69.85

GC content: 31.14

Gene sequence:

>819_bases
ATGAAGTTTTCAAAAATGCATGGAAATGGAAATGATTTTATAGTTATAGAAGATTTAAACAATGAGTATTTAGGTAAAGA
GGGAGAAATTGCTCAAAAAATGTGCCATAGAAGATTTGGAATTGGCGCAGATGGGATTTTAATAGTTAGAAAGAATGAAA
ATTGTGATATAGAAATGGTAATAATAAATTCAGATGGATCTTATGCTGCTATGTGCGGAAATGGAATAAGATGTTTTGCT
AAGTATGTCTACGAGAAGGGAATAGTAAAGAAAGATGTGTTAGATGTTTTAACAGGTGATGGAGTTAAAAGAATCTTTTT
AGAAATTGAGAATGATAAGGTAAAAACTATCAATGTAAATATGGGATTTGGAGACTTTAAACCAAAGAATATTCCAGCAT
TATGTGATGAAGAAATAATAGAAAAAAAAGTAAGTGTAGGAAATGGAAACTTTGAAATAACATCTTTACTTATGGGTGTT
CCTCATACTATTATATTTGAGGAGGAAAAATATCCTATAGAATGTGGTAGGGACATAGAAAAGTATGAATTATTTCCTCA
AGGAACAAATGTTAACTTCTGCAAAGTAATTGATAGAAATACAATGGAAGTTAGAACTTGGGAAAGAGGTGCAGGCCCAA
CCTTAGCGTGTGGAACAGGAAATTGTGCTTCAGTTATAGCTGCTAATAAATTAGGGCTTGTAGATAAAGAAGTAAAGGTA
ATAGTTCCTGGTGGAGAATTAAAAGTTAATATTGAAGATGATGGAGTAAAAATGATAGGTAATGCGTCATTTATTTGTGA
TGGCACATATTTATTTTAG

Upstream 100 bases:

>100_bases
TAAAGCCATGATTTTCTTTCCTTTCTAAATTGATTGAACTTACAGTTAGTATACATCTTATAATAAAAAGTATCAATAAT
AGGTAATTTGAGGTGATAAT

Downstream 100 bases:

>100_bases
AGAGGGAAGTAAGTAAATGAGGAAAGCTGCTGCTTTAAGTTTATTAGTATTAGTTATTTTATCAACAACCCTTGCGGGAT
GTAATTTCGGTAAAGGGTCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKFSKMHGNGNDFIVIEDLNNEYLGKEGEIAQKMCHRRFGIGADGILIVRKNENCDIEMVIINSDGSYAAMCGNGIRCFA
KYVYEKGIVKKDVLDVLTGDGVKRIFLEIENDKVKTINVNMGFGDFKPKNIPALCDEEIIEKKVSVGNGNFEITSLLMGV
PHTIIFEEEKYPIECGRDIEKYELFPQGTNVNFCKVIDRNTMEVRTWERGAGPTLACGTGNCASVIAANKLGLVDKEVKV
IVPGGELKVNIEDDGVKMIGNASFICDGTYLF

Sequences:

>Translated_272_residues
MKFSKMHGNGNDFIVIEDLNNEYLGKEGEIAQKMCHRRFGIGADGILIVRKNENCDIEMVIINSDGSYAAMCGNGIRCFA
KYVYEKGIVKKDVLDVLTGDGVKRIFLEIENDKVKTINVNMGFGDFKPKNIPALCDEEIIEKKVSVGNGNFEITSLLMGV
PHTIIFEEEKYPIECGRDIEKYELFPQGTNVNFCKVIDRNTMEVRTWERGAGPTLACGTGNCASVIAANKLGLVDKEVKV
IVPGGELKVNIEDDGVKMIGNASFICDGTYLF
>Mature_272_residues
MKFSKMHGNGNDFIVIEDLNNEYLGKEGEIAQKMCHRRFGIGADGILIVRKNENCDIEMVIINSDGSYAAMCGNGIRCFA
KYVYEKGIVKKDVLDVLTGDGVKRIFLEIENDKVKTINVNMGFGDFKPKNIPALCDEEIIEKKVSVGNGNFEITSLLMGV
PHTIIFEEEKYPIECGRDIEKYELFPQGTNVNFCKVIDRNTMEVRTWERGAGPTLACGTGNCASVIAANKLGLVDKEVKV
IVPGGELKVNIEDDGVKMIGNASFICDGTYLF

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=275, Percent_Identity=34.5454545454545, Blast_Score=159, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_CLOPE (Q46185)

Other databases:

- EMBL:   BA000016
- EMBL:   X86511
- RefSeq:   NP_562762.1
- ProteinModelPortal:   Q46185
- SMR:   Q46185
- GeneID:   990155
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE1846
- NMPDR:   fig|195102.1.peg.1909
- HOGENOM:   HBG399442
- OMA:   NCASVIA
- PhylomeDB:   Q46185
- ProtClustDB:   PRK00450
- BioCyc:   CPER195102:CPE1846-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30084; Mature: 30084

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 72-72 ACT_SITE 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSKMHGNGNDFIVIEDLNNEYLGKEGEIAQKMCHRRFGIGADGILIVRKNENCDIEMV
CCCCEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEE
IINSDGSYAAMCGNGIRCFAKYVYEKGIVKKDVLDVLTGDGVKRIFLEIENDKVKTINVN
EEECCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECCCEEEEEEEE
MGFGDFKPKNIPALCDEEIIEKKVSVGNGNFEITSLLMGVPHTIIFEEEKYPIECGRDIE
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCC
KYELFPQGTNVNFCKVIDRNTMEVRTWERGAGPTLACGTGNCASVIAANKLGLVDKEVKV
CEEEEECCCCCEEEEEECCCCEEEEEECCCCCCEEEECCCCHHHHEEECCCCCCCCCEEE
IVPGGELKVNIEDDGVKMIGNASFICDGTYLF
EECCCEEEEEECCCCEEEEECCEEEECCEEEC
>Mature Secondary Structure
MKFSKMHGNGNDFIVIEDLNNEYLGKEGEIAQKMCHRRFGIGADGILIVRKNENCDIEMV
CCCCEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEE
IINSDGSYAAMCGNGIRCFAKYVYEKGIVKKDVLDVLTGDGVKRIFLEIENDKVKTINVN
EEECCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECCCEEEEEEEE
MGFGDFKPKNIPALCDEEIIEKKVSVGNGNFEITSLLMGVPHTIIFEEEKYPIECGRDIE
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCC
KYELFPQGTNVNFCKVIDRNTMEVRTWERGAGPTLACGTGNCASVIAANKLGLVDKEVKV
CEEEEECCCCCEEEEEECCCCEEEEEECCCCCCEEEECCCCHHHHEEECCCCCCCCCEEE
IVPGGELKVNIEDDGVKMIGNASFICDGTYLF
EECCCEEEEEECCCCEEEEECCEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842; 7559358