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The map label for this gene is sigK [H]

Identifier: 18310750

GI number: 18310750

Start: 2046088

End: 2046789

Strand: Reverse

Name: sigK [H]

Synonym: CPE1768

Alternate gene names: 18310750

Gene position: 2046789-2046088 (Counterclockwise)

Preceding gene: 18310751

Following gene: 18310749

Centisome position: 67.52

GC content: 29.49

Gene sequence:

>702_bases
ATGTTCATGTTACAATACTTATTAGAATTAGTTGGTAGTAAAATATTTTTAACTGGCTATGTAACAGGAAATTCTACTTT
TCCAAAGCCTTTAAATGAGAAGGAAGAAAAAATCTATTTAGAAAGATTAAAAGATGGAGATGTTGAGGCAAAAAGAGTAC
TAGTTGAGCGGAATTTAAGACTAGTAGCACATATAGTTAAGAAATATTCTTCAAATTACCAAAACTCAAAAGAAATGGAT
GATTTAATATCTATAGGAACAATAGGTTTAATAAAGGCTATAGATTCTTTTGACACTAACAAGGGAATACGACTTGCAAC
ATATGCAGCTAAATGTATAGACAATGAAATACTTATGTTTTTTAGGAATACTAAGAAGACAAAGGGAGAAGTATTCCTTC
AAGATCCTATAGGAGTAGATAAGGAAGGTAATGAAATTTGTCTCATAGATATTTTAAGTAGTGACTCTGATTCGGTATTA
GAGGCTGTTGAAAATTCATTACAGGTTAAGGAACTTTATAAAAAGATGTCAGATGTTTTAAGTCCTAGGGAGAAAGAGAT
AATTCAAATGAGATATGGTCTTTTAGATGGTGATATAAAAACACAGAGGGAAATAGCGGGAATTCTTGGTATATCAAGAT
CATATGTATCTAGAATAGAAAAAAAGGCACTAAAAAAATTAAATAAGGAATTTAAATGTTAA

Upstream 100 bases:

>100_bases
TCAGGGATATAGTTTTAGAACTTACAAAAAATAAATAGATTTAGCAATATTTTAACTATCAACTCATATTATAGTAATAA
GCATTAATAGGGGGAACGAC

Downstream 100 bases:

>100_bases
ATGATACTTAATAAATTGTATAAAAAAAAATAAGTTTTATAAAATTATGGAATTGATATATCATATGGTTTATAATATTT
ATTATACGATAAAAAAGGTG

Product: sporulation sigma factor SigK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MFMLQYLLELVGSKIFLTGYVTGNSTFPKPLNEKEEKIYLERLKDGDVEAKRVLVERNLRLVAHIVKKYSSNYQNSKEMD
DLISIGTIGLIKAIDSFDTNKGIRLATYAAKCIDNEILMFFRNTKKTKGEVFLQDPIGVDKEGNEICLIDILSSDSDSVL
EAVENSLQVKELYKKMSDVLSPREKEIIQMRYGLLDGDIKTQREIAGILGISRSYVSRIEKKALKKLNKEFKC

Sequences:

>Translated_233_residues
MFMLQYLLELVGSKIFLTGYVTGNSTFPKPLNEKEEKIYLERLKDGDVEAKRVLVERNLRLVAHIVKKYSSNYQNSKEMD
DLISIGTIGLIKAIDSFDTNKGIRLATYAAKCIDNEILMFFRNTKKTKGEVFLQDPIGVDKEGNEICLIDILSSDSDSVL
EAVENSLQVKELYKKMSDVLSPREKEIIQMRYGLLDGDIKTQREIAGILGISRSYVSRIEKKALKKLNKEFKC
>Mature_233_residues
MFMLQYLLELVGSKIFLTGYVTGNSTFPKPLNEKEEKIYLERLKDGDVEAKRVLVERNLRLVAHIVKKYSSNYQNSKEMD
DLISIGTIGLIKAIDSFDTNKGIRLATYAAKCIDNEILMFFRNTKKTKGEVFLQDPIGVDKEGNEICLIDILSSDSDSVL
EAVENSLQVKELYKKMSDVLSPREKEIIQMRYGLLDGDIKTQREIAGILGISRSYVSRIEKKALKKLNKEFKC

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=255, Percent_Identity=24.7058823529412, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26559; Mature: 26559

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMLQYLLELVGSKIFLTGYVTGNSTFPKPLNEKEEKIYLERLKDGDVEAKRVLVERNLR
CHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LVAHIVKKYSSNYQNSKEMDDLISIGTIGLIKAIDSFDTNKGIRLATYAAKCIDNEILMF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCHHHHHH
FRNTKKTKGEVFLQDPIGVDKEGNEICLIDILSSDSDSVLEAVENSLQVKELYKKMSDVL
HHCCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
SPREKEIIQMRYGLLDGDIKTQREIAGILGISRSYVSRIEKKALKKLNKEFKC
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFMLQYLLELVGSKIFLTGYVTGNSTFPKPLNEKEEKIYLERLKDGDVEAKRVLVERNLR
CHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LVAHIVKKYSSNYQNSKEMDDLISIGTIGLIKAIDSFDTNKGIRLATYAAKCIDNEILMF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCHHHHHH
FRNTKKTKGEVFLQDPIGVDKEGNEICLIDILSSDSDSVLEAVENSLQVKELYKKMSDVL
HHCCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
SPREKEIIQMRYGLLDGDIKTQREIAGILGISRSYVSRIEKKALKKLNKEFKC
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]