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The map label for this gene is pilT [H]

Identifier: 18310749

GI number: 18310749

Start: 2044855

End: 2045907

Strand: Reverse

Name: pilT [H]

Synonym: CPE1767

Alternate gene names: 18310749

Gene position: 2045907-2044855 (Counterclockwise)

Preceding gene: 18310750

Following gene: 18310748

Centisome position: 67.49

GC content: 31.34

Gene sequence:

>1053_bases
ATGCAAAGTTTAGCAGAACTTTTAGAGTTAACAGTTAAAGAAGGAGCATCTGATTTGCATTTAACAGTAGGTATTTCTCC
TATAATAAAAGTTAATGGAAAATTAGTAAGGTTAGAACATGAAATATTACGCCCTGAAGATACTGAAGCGTATGCTAGAG
AGATATTACAGGATGCATATGAAAAGTATGATGCTATTGGAGAATACGATACATCATATTCAATACATGGTAAAGGTAGA
TTTAGAGTTAATATATATAAACAAAGAAATAGTACAGCTTTAGCTATAAGAGTTATATCTTTAGATATGCCAACTTTGGA
TAGTTTAGGATATCCTGAGACTTTAAAGGATATATGTAATCTTAAAAGAGGACTTGTATTAGTTACAGGACCTACTGGAA
GTGGTAAAAGTACAACTTTAGCAGCTTTAATAAATGAAATAAATAGTAATAGAGAATCTCATATAATAACAATAGAAGAT
CCTATAGAGTTTCTTCATAAACATAATAAATCTATTGTAAACCAAAGAGAGATAGGAAAGGATACTCTAAGTTATGAAAG
AGCCTTAAAAGCAGCTCTTAGAGAGGATCCTGATGTTATTTTAATTGGAGAAATGAGAGATTTAGAGACTATTTCAACAG
CTATAACAGCGGCAGAGACTGGACACTTAGTATTTTCAACTCTTCATACTATAGGAGCTGCTAAGACTATAGATAGAATA
GTAGATGTTTTTCCACCACATCAGCAGGAGCAAATAAAAATACAGTTAGCTTCTGTTTTACAAATAATTATATCACAACA
ATTAGTGGAAACCGTTGATGGAGATAGAAATGCTGCATTAGAAATAATGGTTGCTACACCAGCTATAAAAAATTTAATAA
GAGAAGGAAAAACTCATCAAATAGAATCTTCCATACAAACTGGAAGTAAATATGGAATGAGAACGATGGACATGGAACTT
GCTAATCTGTATAGAGAAGGTATAATAACACAAGAAACGGCAATGAATTCTGCTATAGATAGGGAGATTTTAAGTCGATT
ATTGATGTATTAA

Upstream 100 bases:

>100_bases
ATTATACGATAAAAAAGGTGGATTCTTCTATAAATAATTTGTTCATTTCTTTATGAAGTGAACAAATTATTTATTAAGAA
TTAAGTTTAGGAGGAGATTT

Downstream 100 bases:

>100_bases
ATATAGAGGTAATTTGAATTTTTAAGAGAATATATATATTATCACAATTTTTAGATAGGGAGGTACACATAGAAAAAATG
TCTAAAAACATAGTGGGTCT

Product: twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAREILQDAYEKYDAIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICNLKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQIIISQQLVETVDGDRNAALEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
ANLYREGIITQETAMNSAIDREILSRLLMY

Sequences:

>Translated_350_residues
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAREILQDAYEKYDAIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICNLKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQIIISQQLVETVDGDRNAALEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
ANLYREGIITQETAMNSAIDREILSRLLMY
>Mature_350_residues
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAREILQDAYEKYDAIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICNLKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQIIISQQLVETVDGDRNAALEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
ANLYREGIITQETAMNSAIDREILSRLLMY

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=330, Percent_Identity=43.030303030303, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI1789723, Length=170, Percent_Identity=41.7647058823529, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786296, Length=198, Percent_Identity=38.3838383838384, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39030; Mature: 39030

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAREILQDAY
CHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCEEEEEEHHHCCCCCHHHHHHHHHHHHH
EKYDAIGEYDTSYSIHGKGRFRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICN
HHHHCCCCCCCCEEEECCCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHH
LKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIEDPIEFLHKHNKSIVNQREIGK
HCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCHHHHHHHHCCH
DTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VDVFPPHQQEQIKIQLASVLQIIISQQLVETVDGDRNAALEIMVATPAIKNLIREGKTHQ
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHCCCCHH
IESSIQTGSKYGMRTMDMELANLYREGIITQETAMNSAIDREILSRLLMY
HHHHHHCCHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAREILQDAY
CHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCEEEEEEHHHCCCCCHHHHHHHHHHHHH
EKYDAIGEYDTSYSIHGKGRFRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICN
HHHHCCCCCCCCEEEECCCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHH
LKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIEDPIEFLHKHNKSIVNQREIGK
HCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCHHHHHHHHCCH
DTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VDVFPPHQQEQIKIQLASVLQIIISQQLVETVDGDRNAALEIMVATPAIKNLIREGKTHQ
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHCCCCHH
IESSIQTGSKYGMRTMDMELANLYREGIITQETAMNSAIDREILSRLLMY
HHHHHHCCHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]