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The map label for this gene is priA [H]

Identifier: 18310727

GI number: 18310727

Start: 2021654

End: 2023849

Strand: Reverse

Name: priA [H]

Synonym: CPE1745

Alternate gene names: 18310727

Gene position: 2023849-2021654 (Counterclockwise)

Preceding gene: 18310728

Following gene: 18310726

Centisome position: 66.76

GC content: 27.64

Gene sequence:

>2196_bases
TTGGAGAAATTTGCTGAAGTAATTGTAAATAGTGAAGCTGTTACAATTGATAAGCCTTTTACATATAAGATTAAAGAAGA
TTTAGTTCATAATATAAAGGTTGGACATAGAGTTTTAATTCCTTTTGGAAATGGAAATAAAAAAATTGAAGGTTTTGTTT
TAAAAATATTAGATAATGTTGAAAATAAAAGTATAAGATATAAATCTATATATAATGTTTGTGATAATGAACCCTTATTA
AGTGAAGATTCTTTAAAGCTTATTAAATTTTTAAGAGAGAAATATTTATGTAAGTATATAGATGCTATAAGAGTTATGAT
TCCACCTAAAATAATGAGTGGAAATAAGGAAAAAACAAAACAAGTTATAGTATTTAAAAAATATATAGAAGAATTAAGTG
AGCCACAAAAAAAAGCTTTAGAGCTAATTGAAGAAAAAAGTGGTGGTTTCACAAAAGCAGAATGGAATAAAATTTTTAAT
ATATCAACATACATGATAAATAAATTAATAGAATTAGGTTGTGCTTCTAGTGAAGAAGTAAGGGTTGGAAGGGAAAATTT
AAGAGAGTTTAAGCCTTATCCGCCTAAAAGCTTAAATGAAGAACAGATAAGAGCATATGATACAATTTTAGAAAGTGATA
AAAATATATTTTTATTAAAGGGCGTAACTGGAAGTGGAAAAACAGAAGTATATATGAATTTAGTTAGTTATATGCTCCTT
TTTGAAAAGAGTTCATTAATATTAGTACCAGAAATAGCCCTAACTCCTCAAATGATTGAAAGGTTTAAAGGAAGATTTGG
AAAAGATGTGGCAGTATTTCATAGTAGGCTTTCAGATGGAGAGAGATATGATGAATGGTATAGGGTTAAAAATGATCAAG
TTAAGGTTGTTATAGGAGCAAGATCAGCAATATTTTTACCTTTTAATGATTTAGGACTAATAGTAGTAGATGAAGAACAT
GAAAGTAGTTATAAATCAGATATGAATCCTAAATATAATACTATAGAAGTTTGTGAGTATTTAAGTCTCCTAAAAAATTG
TAAGGTTGTTTTAGGTTCAGCAACCCCTAGTGTTGTTAGTTTTTATAAAGCACTTAAAGGGAATTATGAATTAATTGAAC
TTAAAAAGAGAGTTAATAATTTAGAACTTCCTAATGTATCTATTGTGGATATGAGGGAAGAGCTAAAATCAAATAATAAA
ACAATGTTTAGTAGGGATTTATATTTAAAGATAAAAGATAGATTAGAGAAAAAAGAACAAATAATTTTGTTTTTAAATAG
AAGAGGATTTTCATCCTTTGTTTCTTGTAGAAGTTGTGGATTTGTTTTTAAATGTAACAATTGTGACATATCAATGACTT
ATCATAATAATGGATACTTAGTTTGTCATTATTGTGGAAATACTGAGAAGATGCCTAAACTTTGCCCTAAGTGTGGCAGT
AACTATGTTAAACATTTTGGGGTAGGAACAGAAAAATTAGAGGAAGCAGTACGTCATTATTTCAAAGAAGCAAAGGTTTT
AAGGATGGATGTAGATACAACAAGAAAGAAAAATTCTCATGAGGAAATTTATAACTCATTTAAAAGTAAAGAAGCAGATA
TATTAATAGGAACTCAGATGATAGCTAAGGGGCTTGATTTCCCCGATGTAACCTTAGTTGGAGTTATGGCTGCAGATATG
TCTTTAAATTTGCCAGATTATAAGTCTAGTGAAAAGACATTTCAACTTATAACTCAGGTAAGTGGTAGAGCAGGACGTGG
TGATAAAAAGGGAGATGTAATAATTCAAAGTTATTCTCCAGAACATTATGCTTTGACCTGTGCAAGGGATAATAATTATG
AAGAGTTCTTTAAAAGAGAAATAAATTTAAGAAAACTTATGGCATATCCACCCTTTGATGATTTGTTTTTAATAAACTTA
AGTGCAAAGGGAGAAATAAAAGTAAAGATTTTTGCAAAAAAATTAGAGGCGTATTTACGTAATATATTAAAAGAATATGA
TAATATATTTATATACGAAGCATGCCCTTGTTCTATAAGTAAAATAAAAAATGTTTATAGATGGCAAATTCTAATTAAGG
GAAAATTAGATTTAGATGTATGCTCTAAAATAAAAAAAGGGGTTTATGAATTATCCAAAGATGTTTATAATGAGATTAAG
ATTGGTTTAGATATAAATCCTAATAGCTTAATGTAA

Upstream 100 bases:

>100_bases
TAGAAAAGATGTCTAAAAGAGAAGTTGCAAAGAATATATTTGACATTATAAAAGGGCGCTAATTGCGCCTTTTTATTCTA
TATGAACAAGGGTGATTATT

Downstream 100 bases:

>100_bases
GGAGGAAGTTATTTTGGCAATAAGAAACTTAAGATTTAATGACGACGAAATATTAAGAAAAAAATGTAGAGTAGTTGATG
ACATTAATGATAGAATAAAA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MEKFAEVIVNSEAVTIDKPFTYKIKEDLVHNIKVGHRVLIPFGNGNKKIEGFVLKILDNVENKSIRYKSIYNVCDNEPLL
SEDSLKLIKFLREKYLCKYIDAIRVMIPPKIMSGNKEKTKQVIVFKKYIEELSEPQKKALELIEEKSGGFTKAEWNKIFN
ISTYMINKLIELGCASSEEVRVGRENLREFKPYPPKSLNEEQIRAYDTILESDKNIFLLKGVTGSGKTEVYMNLVSYMLL
FEKSSLILVPEIALTPQMIERFKGRFGKDVAVFHSRLSDGERYDEWYRVKNDQVKVVIGARSAIFLPFNDLGLIVVDEEH
ESSYKSDMNPKYNTIEVCEYLSLLKNCKVVLGSATPSVVSFYKALKGNYELIELKKRVNNLELPNVSIVDMREELKSNNK
TMFSRDLYLKIKDRLEKKEQIILFLNRRGFSSFVSCRSCGFVFKCNNCDISMTYHNNGYLVCHYCGNTEKMPKLCPKCGS
NYVKHFGVGTEKLEEAVRHYFKEAKVLRMDVDTTRKKNSHEEIYNSFKSKEADILIGTQMIAKGLDFPDVTLVGVMAADM
SLNLPDYKSSEKTFQLITQVSGRAGRGDKKGDVIIQSYSPEHYALTCARDNNYEEFFKREINLRKLMAYPPFDDLFLINL
SAKGEIKVKIFAKKLEAYLRNILKEYDNIFIYEACPCSISKIKNVYRWQILIKGKLDLDVCSKIKKGVYELSKDVYNEIK
IGLDINPNSLM

Sequences:

>Translated_731_residues
MEKFAEVIVNSEAVTIDKPFTYKIKEDLVHNIKVGHRVLIPFGNGNKKIEGFVLKILDNVENKSIRYKSIYNVCDNEPLL
SEDSLKLIKFLREKYLCKYIDAIRVMIPPKIMSGNKEKTKQVIVFKKYIEELSEPQKKALELIEEKSGGFTKAEWNKIFN
ISTYMINKLIELGCASSEEVRVGRENLREFKPYPPKSLNEEQIRAYDTILESDKNIFLLKGVTGSGKTEVYMNLVSYMLL
FEKSSLILVPEIALTPQMIERFKGRFGKDVAVFHSRLSDGERYDEWYRVKNDQVKVVIGARSAIFLPFNDLGLIVVDEEH
ESSYKSDMNPKYNTIEVCEYLSLLKNCKVVLGSATPSVVSFYKALKGNYELIELKKRVNNLELPNVSIVDMREELKSNNK
TMFSRDLYLKIKDRLEKKEQIILFLNRRGFSSFVSCRSCGFVFKCNNCDISMTYHNNGYLVCHYCGNTEKMPKLCPKCGS
NYVKHFGVGTEKLEEAVRHYFKEAKVLRMDVDTTRKKNSHEEIYNSFKSKEADILIGTQMIAKGLDFPDVTLVGVMAADM
SLNLPDYKSSEKTFQLITQVSGRAGRGDKKGDVIIQSYSPEHYALTCARDNNYEEFFKREINLRKLMAYPPFDDLFLINL
SAKGEIKVKIFAKKLEAYLRNILKEYDNIFIYEACPCSISKIKNVYRWQILIKGKLDLDVCSKIKKGVYELSKDVYNEIK
IGLDINPNSLM
>Mature_731_residues
MEKFAEVIVNSEAVTIDKPFTYKIKEDLVHNIKVGHRVLIPFGNGNKKIEGFVLKILDNVENKSIRYKSIYNVCDNEPLL
SEDSLKLIKFLREKYLCKYIDAIRVMIPPKIMSGNKEKTKQVIVFKKYIEELSEPQKKALELIEEKSGGFTKAEWNKIFN
ISTYMINKLIELGCASSEEVRVGRENLREFKPYPPKSLNEEQIRAYDTILESDKNIFLLKGVTGSGKTEVYMNLVSYMLL
FEKSSLILVPEIALTPQMIERFKGRFGKDVAVFHSRLSDGERYDEWYRVKNDQVKVVIGARSAIFLPFNDLGLIVVDEEH
ESSYKSDMNPKYNTIEVCEYLSLLKNCKVVLGSATPSVVSFYKALKGNYELIELKKRVNNLELPNVSIVDMREELKSNNK
TMFSRDLYLKIKDRLEKKEQIILFLNRRGFSSFVSCRSCGFVFKCNNCDISMTYHNNGYLVCHYCGNTEKMPKLCPKCGS
NYVKHFGVGTEKLEEAVRHYFKEAKVLRMDVDTTRKKNSHEEIYNSFKSKEADILIGTQMIAKGLDFPDVTLVGVMAADM
SLNLPDYKSSEKTFQLITQVSGRAGRGDKKGDVIIQSYSPEHYALTCARDNNYEEFFKREINLRKLMAYPPFDDLFLINL
SAKGEIKVKIFAKKLEAYLRNILKEYDNIFIYEACPCSISKIKNVYRWQILIKGKLDLDVCSKIKKGVYELSKDVYNEIK
IGLDINPNSLM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=737, Percent_Identity=30.8005427408412, Blast_Score=386, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 84250; Mature: 84250

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFAEVIVNSEAVTIDKPFTYKIKEDLVHNIKVGHRVLIPFGNGNKKIEGFVLKILDNV
CCCHHHHHCCCCEEEECCCEEEHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCC
ENKSIRYKSIYNVCDNEPLLSEDSLKLIKFLREKYLCKYIDAIRVMIPPKIMSGNKEKTK
CCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHH
QVIVFKKYIEELSEPQKKALELIEEKSGGFTKAEWNKIFNISTYMINKLIELGCASSEEV
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHEEEHHHHHHHHHHHHCCCCCHHH
RVGRENLREFKPYPPKSLNEEQIRAYDTILESDKNIFLLKGVTGSGKTEVYMNLVSYMLL
HHCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
FEKSSLILVPEIALTPQMIERFKGRFGKDVAVFHSRLSDGERYDEWYRVKNDQVKVVIGA
HCCCCEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHEECCCCEEEEEEC
RSAIFLPFNDLGLIVVDEEHESSYKSDMNPKYNTIEVCEYLSLLKNCKVVLGSATPSVVS
CEEEEEEECCCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHH
FYKALKGNYELIELKKRVNNLELPNVSIVDMREELKSNNKTMFSRDLYLKIKDRLEKKEQ
HHHHHCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCCCEEEECCEEEEEHHHCCCCCE
IILFLNRRGFSSFVSCRSCGFVFKCNNCDISMTYHNNGYLVCHYCGNTEKMPKLCPKCGS
EEEEEECCCCHHHHHHHHCCEEEEECCCEEEEEEECCCEEEEEECCCCCCHHHHHHHHCC
NYVKHFGVGTEKLEEAVRHYFKEAKVLRMDVDTTRKKNSHEEIYNSFKSKEADILIGTQM
HHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEHHH
IAKGLDFPDVTLVGVMAADMSLNLPDYKSSEKTFQLITQVSGRAGRGDKKGDVIIQSYSP
HHCCCCCCCHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCC
EHYALTCARDNNYEEFFKREINLRKLMAYPPFDDLFLINLSAKGEIKVKIFAKKLEAYLR
CEEEEEEECCCCHHHHHHHHCCHHHEECCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHH
NILKEYDNIFIYEACPCSISKIKNVYRWQILIKGKLDLDVCSKIKKGVYELSKDVYNEIK
HHHHHHCCEEEEEECCCCHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEE
IGLDINPNSLM
EEEECCCCCCC
>Mature Secondary Structure
MEKFAEVIVNSEAVTIDKPFTYKIKEDLVHNIKVGHRVLIPFGNGNKKIEGFVLKILDNV
CCCHHHHHCCCCEEEECCCEEEHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCC
ENKSIRYKSIYNVCDNEPLLSEDSLKLIKFLREKYLCKYIDAIRVMIPPKIMSGNKEKTK
CCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHH
QVIVFKKYIEELSEPQKKALELIEEKSGGFTKAEWNKIFNISTYMINKLIELGCASSEEV
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHEEEHHHHHHHHHHHHCCCCCHHH
RVGRENLREFKPYPPKSLNEEQIRAYDTILESDKNIFLLKGVTGSGKTEVYMNLVSYMLL
HHCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
FEKSSLILVPEIALTPQMIERFKGRFGKDVAVFHSRLSDGERYDEWYRVKNDQVKVVIGA
HCCCCEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHEECCCCEEEEEEC
RSAIFLPFNDLGLIVVDEEHESSYKSDMNPKYNTIEVCEYLSLLKNCKVVLGSATPSVVS
CEEEEEEECCCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHH
FYKALKGNYELIELKKRVNNLELPNVSIVDMREELKSNNKTMFSRDLYLKIKDRLEKKEQ
HHHHHCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCCCEEEECCEEEEEHHHCCCCCE
IILFLNRRGFSSFVSCRSCGFVFKCNNCDISMTYHNNGYLVCHYCGNTEKMPKLCPKCGS
EEEEEECCCCHHHHHHHHCCEEEEECCCEEEEEEECCCEEEEEECCCCCCHHHHHHHHCC
NYVKHFGVGTEKLEEAVRHYFKEAKVLRMDVDTTRKKNSHEEIYNSFKSKEADILIGTQM
HHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEHHH
IAKGLDFPDVTLVGVMAADMSLNLPDYKSSEKTFQLITQVSGRAGRGDKKGDVIIQSYSP
HHCCCCCCCHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCC
EHYALTCARDNNYEEFFKREINLRKLMAYPPFDDLFLINLSAKGEIKVKIFAKKLEAYLR
CEEEEEEECCCCHHHHHHHHCCHHHEECCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHH
NILKEYDNIFIYEACPCSISKIKNVYRWQILIKGKLDLDVCSKIKKGVYELSKDVYNEIK
HHHHHHCCEEEEEECCCCHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEE
IGLDINPNSLM
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]