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The map label for this gene is rlmN

Identifier: 18310722

GI number: 18310722

Start: 2017121

End: 2018164

Strand: Reverse

Name: rlmN

Synonym: CPE1740

Alternate gene names: 18310722

Gene position: 2018164-2017121 (Counterclockwise)

Preceding gene: 18310723

Following gene: 18310721

Centisome position: 66.57

GC content: 30.08

Gene sequence:

>1044_bases
ATGAAAAACATCTTAGATTTTACATTAGAAGAGTTAAAAGAGTGGATGAAGGAAAATGGAGAGAGTGCTTTTAGAGCAAA
GCAAATCTTTGATTGGATATACAAAAAGGAAGTATTTAATTTTGAAGAGATGAAAAATATTTCAAAAGCTCTAATAGGCA
AGCTTTCAGAAAATTTTTATATAGGTATCCCTGAAGTTATAGATTATTTATCATCTAGTGAAGATGGTACTAGAAAAATC
CTTTTAGGATTAGGTGATGGAAACATAATAGAATGTGTTATTATGAGATATAAATATGGTAATTCTATTTGTGTTTCCAC
ACAAATAGGATGTAGAATGGGATGTAAATTCTGTGCATCTACCTTAGAAGGAATGGTAAGAAATTTAACTGCAGGAGAAA
TATTATCAGAAGTTTTAATTGGACAAAAACTTCTAGGTGAGAGAATATCTAATATAGTTTTAATGGGAAGTGGAGAACCC
TTAGACAATTATGATAATGTTATGAAGTTCTTAGAGCTTGTCAATGCTGATTATGGCTTAAATATAGGGCAAAGACATAT
AACATTATCAACATGTGGTCTTGTGCCAAAAATTCGTGAAATGGCTGATAAGGAAATGCAAGTAACTTTAGCAATTTCAT
TACATGCTGTTTCAGATGAAAAGAGAAAAACAATAATGCCAATAGCTAATAAGTATTCAATTAGTGAAATTTTAGACGCT
TGTAATTATTATATTGAGAAAACAGGAAGAAGAATAACTTTTGAATATTCTTTAGTTAGTGGAGTTAACGATACTAAAGA
AGATGCCAAGAGCTTAGGAAGATTATTAAAAGGCATGCTTTGTCATGTTAATTTAATTCCTGTAAATGAAATAAAGGAAA
ATGAGTTTAAAAAATCTACTAAGAAGGATATAGAAACATTTTTAAATACTCTTAAAACATATGGAGTAGAAGCTACAGTA
AGAAGAGAGATGGGCTCAGATATAAATGCAGCCTGTGGTCAATTGAGAAGAAGCTATATTAAGTCAAAAGGGTTAAAGCT
GTAG

Upstream 100 bases:

>100_bases
ACCATATTACCTAATAAGTACATGGATGGTTTCTTTATTGCTAAGCTTAAGAAAAAAGAAAGTAAATAGCAAAGAGGATA
AACATGGAAGGTGAGACTGT

Downstream 100 bases:

>100_bases
GGGGAAATATTTATGTTAGGATTTGTAACAGATAAAGGAAATGTTAGAGAGTTAAACGAAGATTTTTTATCTTATTCTAG
AGAAGATGAATTTTCAATAT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKNILDFTLEELKEWMKENGESAFRAKQIFDWIYKKEVFNFEEMKNISKALIGKLSENFYIGIPEVIDYLSSSEDGTRKI
LLGLGDGNIIECVIMRYKYGNSICVSTQIGCRMGCKFCASTLEGMVRNLTAGEILSEVLIGQKLLGERISNIVLMGSGEP
LDNYDNVMKFLELVNADYGLNIGQRHITLSTCGLVPKIREMADKEMQVTLAISLHAVSDEKRKTIMPIANKYSISEILDA
CNYYIEKTGRRITFEYSLVSGVNDTKEDAKSLGRLLKGMLCHVNLIPVNEIKENEFKKSTKKDIETFLNTLKTYGVEATV
RREMGSDINAACGQLRRSYIKSKGLKL

Sequences:

>Translated_347_residues
MKNILDFTLEELKEWMKENGESAFRAKQIFDWIYKKEVFNFEEMKNISKALIGKLSENFYIGIPEVIDYLSSSEDGTRKI
LLGLGDGNIIECVIMRYKYGNSICVSTQIGCRMGCKFCASTLEGMVRNLTAGEILSEVLIGQKLLGERISNIVLMGSGEP
LDNYDNVMKFLELVNADYGLNIGQRHITLSTCGLVPKIREMADKEMQVTLAISLHAVSDEKRKTIMPIANKYSISEILDA
CNYYIEKTGRRITFEYSLVSGVNDTKEDAKSLGRLLKGMLCHVNLIPVNEIKENEFKKSTKKDIETFLNTLKTYGVEATV
RREMGSDINAACGQLRRSYIKSKGLKL
>Mature_347_residues
MKNILDFTLEELKEWMKENGESAFRAKQIFDWIYKKEVFNFEEMKNISKALIGKLSENFYIGIPEVIDYLSSSEDGTRKI
LLGLGDGNIIECVIMRYKYGNSICVSTQIGCRMGCKFCASTLEGMVRNLTAGEILSEVLIGQKLLGERISNIVLMGSGEP
LDNYDNVMKFLELVNADYGLNIGQRHITLSTCGLVPKIREMADKEMQVTLAISLHAVSDEKRKTIMPIANKYSISEILDA
CNYYIEKTGRRITFEYSLVSGVNDTKEDAKSLGRLLKGMLCHVNLIPVNEIKENEFKKSTKKDIETFLNTLKTYGVEATV
RREMGSDINAACGQLRRSYIKSKGLKL

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=343, Percent_Identity=40.5247813411079, Blast_Score=255, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_CLOP1 (Q0TPL4)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_696426.1
- ProteinModelPortal:   Q0TPL4
- STRING:   Q0TPL4
- GeneID:   4201008
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_1993
- TIGR:   CPF_1993
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14454
- BioCyc:   CPER195103:CPF_1993-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39164; Mature: 39164

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNILDFTLEELKEWMKENGESAFRAKQIFDWIYKKEVFNFEEMKNISKALIGKLSENFY
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEE
IGIPEVIDYLSSSEDGTRKILLGLGDGNIIECVIMRYKYGNSICVSTQIGCRMGCKFCAS
EECHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHH
TLEGMVRNLTAGEILSEVLIGQKLLGERISNIVLMGSGEPLDNYDNVMKFLELVNADYGL
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCC
NIGQRHITLSTCGLVPKIREMADKEMQVTLAISLHAVSDEKRKTIMPIANKYSISEILDA
CCCCEEEEEHHHCCCHHHHHHCCCCEEEEEEEEEEECCCCHHHHHCCCCCCCCHHHHHHH
CNYYIEKTGRRITFEYSLVSGVNDTKEDAKSLGRLLKGMLCHVNLIPVNEIKENEFKKST
HHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHH
KKDIETFLNTLKTYGVEATVRREMGSDINAACGQLRRSYIKSKGLKL
HHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNILDFTLEELKEWMKENGESAFRAKQIFDWIYKKEVFNFEEMKNISKALIGKLSENFY
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEE
IGIPEVIDYLSSSEDGTRKILLGLGDGNIIECVIMRYKYGNSICVSTQIGCRMGCKFCAS
EECHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHH
TLEGMVRNLTAGEILSEVLIGQKLLGERISNIVLMGSGEPLDNYDNVMKFLELVNADYGL
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCC
NIGQRHITLSTCGLVPKIREMADKEMQVTLAISLHAVSDEKRKTIMPIANKYSISEILDA
CCCCEEEEEHHHCCCHHHHHHCCCCEEEEEEEEEEECCCCHHHHHCCCCCCCCHHHHHHH
CNYYIEKTGRRITFEYSLVSGVNDTKEDAKSLGRLLKGMLCHVNLIPVNEIKENEFKKST
HHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHH
KKDIETFLNTLKTYGVEATVRREMGSDINAACGQLRRSYIKSKGLKL
HHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA