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The map label for this gene is trmD [H]

Identifier: 18310691

GI number: 18310691

Start: 1987985

End: 1988695

Strand: Reverse

Name: trmD [H]

Synonym: CPE1709

Alternate gene names: 18310691

Gene position: 1988695-1987985 (Counterclockwise)

Preceding gene: 18310692

Following gene: 18310690

Centisome position: 65.6

GC content: 31.08

Gene sequence:

>711_bases
ATGAAGATTAATATTCTTACATTGTTCCCAGAGATGTTTGATATATTTAAACATAGCATAATAGGAAGAGCTAGGGAAAA
TGGTTTTCTACATATAGAGACTATAAATATAAGAGATTATACTTTAAATAAGCATAAAAAGGTAGATGATTATCCTTATG
GGGGAGGAGCAGGAATGGTTATGACTCCTCAGCCAGTTGTAGATGCTATAAAGGCTGTTAAAGAAAAAAATAAGGGTAAA
GTTATATTTTTAGGACCAAGAGGTAAAACTTTTAATCAAGAAATGGCTAAGGAACTTTCTAAGGAGGAAGAATTAGTTTT
TGTCTGTGGACACTATGAAGGTATAGATCAAAGAGTTTATAAATATTTTGATTTAGAAATTTCTCTTGGAGATTTTGTGT
TAACAGGTGGAGAAATGGCATGTATTCCAGTTATTGATAGCATATCTAGATTAGTGCCAGGTGTTTTAGGAAGTGAAGAG
AGTTTCCAAGATGAAAGTTATTATGATGGGACTTTAGAATATCCACAATATACAAGACCTTTTGAATTTGAAGGAGAGAA
AGTTCCAGAGGTTCTTATGTCAGGTCATCACGAAAATATAAGAAAGTGGAGAAGAAAACAATCACTTCTTATAACTAAGG
AAAGAAGACCAGATATGTTTGAAAAAATTAAACTCTCAAAAGAAGACATAAAATTGTTAAAATCAAAATAA

Upstream 100 bases:

>100_bases
GGAAAGAAGGAAGTATTAGTTCCAGTATTAAAGGATATAGTTTTAGATATTAATATGGATGAAAAATTAATTACTATAAG
ACCTTCTGGAGAGTGGCAAT

Downstream 100 bases:

>100_bases
ATATATTGATTAAAGAATACAAGTATGATAGAATAAACTTCGTGCTAGTACCGGCGGTCCTCTGTCACAGAGTGTGTTAA
GAACGTCAAAATTTTAGAAG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKINILTLFPEMFDIFKHSIIGRARENGFLHIETINIRDYTLNKHKKVDDYPYGGGAGMVMTPQPVVDAIKAVKEKNKGK
VIFLGPRGKTFNQEMAKELSKEEELVFVCGHYEGIDQRVYKYFDLEISLGDFVLTGGEMACIPVIDSISRLVPGVLGSEE
SFQDESYYDGTLEYPQYTRPFEFEGEKVPEVLMSGHHENIRKWRRKQSLLITKERRPDMFEKIKLSKEDIKLLKSK

Sequences:

>Translated_236_residues
MKINILTLFPEMFDIFKHSIIGRARENGFLHIETINIRDYTLNKHKKVDDYPYGGGAGMVMTPQPVVDAIKAVKEKNKGK
VIFLGPRGKTFNQEMAKELSKEEELVFVCGHYEGIDQRVYKYFDLEISLGDFVLTGGEMACIPVIDSISRLVPGVLGSEE
SFQDESYYDGTLEYPQYTRPFEFEGEKVPEVLMSGHHENIRKWRRKQSLLITKERRPDMFEKIKLSKEDIKLLKSK
>Mature_236_residues
MKINILTLFPEMFDIFKHSIIGRARENGFLHIETINIRDYTLNKHKKVDDYPYGGGAGMVMTPQPVVDAIKAVKEKNKGK
VIFLGPRGKTFNQEMAKELSKEEELVFVCGHYEGIDQRVYKYFDLEISLGDFVLTGGEMACIPVIDSISRLVPGVLGSEE
SFQDESYYDGTLEYPQYTRPFEFEGEKVPEVLMSGHHENIRKWRRKQSLLITKERRPDMFEKIKLSKEDIKLLKSK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=237, Percent_Identity=48.1012658227848, Blast_Score=222, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27262; Mature: 27262

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINILTLFPEMFDIFKHSIIGRARENGFLHIETINIRDYTLNKHKKVDDYPYGGGAGMV
CEEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCEE
MTPQPVVDAIKAVKEKNKGKVIFLGPRGKTFNQEMAKELSKEEELVFVCGHYEGIDQRVY
ECCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHH
KYFDLEISLGDFVLTGGEMACIPVIDSISRLVPGVLGSEESFQDESYYDGTLEYPQYTRP
HHEEEEEECCCEEEECCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCC
FEFEGEKVPEVLMSGHHENIRKWRRKQSLLITKERRPDMFEKIKLSKEDIKLLKSK
CCCCCCHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MKINILTLFPEMFDIFKHSIIGRARENGFLHIETINIRDYTLNKHKKVDDYPYGGGAGMV
CEEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCEE
MTPQPVVDAIKAVKEKNKGKVIFLGPRGKTFNQEMAKELSKEEELVFVCGHYEGIDQRVY
ECCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHH
KYFDLEISLGDFVLTGGEMACIPVIDSISRLVPGVLGSEESFQDESYYDGTLEYPQYTRP
HHEEEEEECCCEEEECCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCC
FEFEGEKVPEVLMSGHHENIRKWRRKQSLLITKERRPDMFEKIKLSKEDIKLLKSK
CCCCCCHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA