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The map label for this gene is topA [H]

Identifier: 18310684

GI number: 18310684

Start: 1980439

End: 1982541

Strand: Reverse

Name: topA [H]

Synonym: CPE1702

Alternate gene names: 18310684

Gene position: 1982541-1980439 (Counterclockwise)

Preceding gene: 18310685

Following gene: 18310683

Centisome position: 65.4

GC content: 30.58

Gene sequence:

>2103_bases
ATGGGTCAAAAATTAGTAATTGTTGAGTCACCAGCTAAAGCCAAGACAATAGAAAAATATTTAGGTAAAAATTATGTAGT
CGAAGCTTCCATGGGACATGTAAGAGATTTGCCAAAAAGCCAATTAGGCGTTGATATAGAAAATGAATATAATCCTAAAT
ATATAACAATAAGAGGAAAAGGTGAACTTTTAAGCAAACTTAGAAAGTTAGCAAAAAAAAGTGATAAGATATACCTTGCA
ACTGACCCTGATAGAGAAGGGGAAGCTATATCTTGGCACTTAGCTAATGTGCTTAAGATAGATGAAAACGAGAATTGTAG
AATAGAATTTAATGAAATAACAAAGGATGCAGTTAAAAATTCAATAAAGCATCCAAGAAAAATAAATTGTAATTTAGTAG
ATGCTCAGCAAGCAAGAAGAGTGTTAGACAGATTGGTTGGATATGAAATAAGTCCACTTCTATGGAGAAATGTAAAATGG
GGATTGAGTGCTGGAAGGGTTCAGTCAGCAGCATTAAAACTTATATGTGATAGAGAAGAAGAAATAAAGAAGTTCAATCC
AGAAGAGTATTGGACTGTTGATGTTAAACTTAAAAAAGGAAAGAAGTCTTTTCCTGTTAAGTTAACAACTAAAAATAAGA
AAAAGATAGAAATAAAAAATAAAGAACAAGCAGATCAAATAATAGATGAACTTAAAGAAAATGAATATATAGTAAGCAAG
ATAAAAAAAGGAACTAAAAATAAAAATCCTTTAGCTCCTTTTACTACAAGTACTCTTCAACAAGAGGCAAGTAAAAAGCT
TAACTTCATGACAAAGAAAACAATGTCAGTAGCTCAACAACTTTATGAAGGGGTTGAAGTTAAGAAATTTGGAACTGTAG
GTTTAATAACTTATATGAGAACTGATTCTGTTAGAATCTCAAAGGAAGCTCAAGAAAAAGCTCTTAACTTTATAGATGAA
ACATATGGAAAAGAGTATGTTCCAGAGGAATCAAGAGTATATAAAGGAAAGAAAAATATACAAGATGCTCATGAAGCCAT
AAGACCTACATATGTTGAAATAACACCAGAGATTGCTAAGGCAAACTTAAGTAATGATCAATATAAGTTATATGCTTTAA
TATGGAAAAGATTTATAGCAAGCCAAATGGCTACATGTATATTAAATACTAATAGCTTAGAGATTAAAAATGGTGATTAT
ACATTAAGAGCTAGTGGATCTACTATAAAATTTGATGGATTCATGAAAGTTTATGAATATATATCAGGGGAAGAGGAAGA
ATCAGTACTTTTACCTGAATTAGAGGAAAATGAAGTTTTAAAAGAAGAATCAATTGAAGGTAAACAACATTTTACTCAAC
CACCTGCTAGATATTCAGAGGCTGCTTTTGTTAAGCTTTTAGAGGAAAAAGGTATTGGAAGACCAAGTACTTACGTTCCA
ACAATATCTACATTAATAAGTAGAAAATATGTAGATAGAGAGAAAAAGATTCTTATACCTACAGAATTAGGCTTTATAGT
AAATGATATACTTTCAAATTATTTTAAGCAGATAGTTGATACTGACTTTACTGCTGAAATGGAAGTTAAACTTGATAATG
TTGAAGCCGGAAAAGAAAGTTGGACTCATATAGTAGATGAATTCTTTACTCCATTAAAGGAGGATATAGAAAAGGCAGAA
AAAGAGATATCTAAGGTTATTATAGAAGATAAAGTCAGTGATGTACCTTGTGATAAATGTGGAAGACTTATGGTTATTAA
GCATGGAAGATTTGGAGATTTCTTAGCTTGTCCTGGATATCCAGAGTGTCAAAACACAAAACCTATAGTTGAAGAGGTTG
ATGCAAACTGTCCATTATGTGGAGGAAAGATTTTAGTTAAGAGAAGTAAAAAGGGAAACAGATTCTATGGATGTAGTAAT
TATCCAGAGTGTAATTTCGTAAGTTGGTATGAACCAACAAATGAAAAATGTCCAGAATGTGGTTCATATATGGTTAAGAG
ATACTCTAAGAGTAAAGGTGAATATTTACAGTGTAGTGATAAAGAATGTAAATATGAAAAAATAATTGAAAAAAATAATG
ATGAAAATAACTCAGAAAAGTAG

Upstream 100 bases:

>100_bases
AATTTGAAAATAAAATAGAAGTTATACCAGGAGATTATTATACAAAAATAATATGATAGTTTGAGGGGGTGAACACTCTG
GATTTAATTCAGAGTTTACG

Downstream 100 bases:

>100_bases
TTTTTAGTCCTTATCTCCACCAGTATTACTGGTTGAAATGAAGTATTTACTATGTTAATATTTAAATAATAAGTTTATTA
TAAGAATATTTTAAAAATTC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 700; Mature: 699

Protein sequence:

>700_residues
MGQKLVIVESPAKAKTIEKYLGKNYVVEASMGHVRDLPKSQLGVDIENEYNPKYITIRGKGELLSKLRKLAKKSDKIYLA
TDPDREGEAISWHLANVLKIDENENCRIEFNEITKDAVKNSIKHPRKINCNLVDAQQARRVLDRLVGYEISPLLWRNVKW
GLSAGRVQSAALKLICDREEEIKKFNPEEYWTVDVKLKKGKKSFPVKLTTKNKKKIEIKNKEQADQIIDELKENEYIVSK
IKKGTKNKNPLAPFTTSTLQQEASKKLNFMTKKTMSVAQQLYEGVEVKKFGTVGLITYMRTDSVRISKEAQEKALNFIDE
TYGKEYVPEESRVYKGKKNIQDAHEAIRPTYVEITPEIAKANLSNDQYKLYALIWKRFIASQMATCILNTNSLEIKNGDY
TLRASGSTIKFDGFMKVYEYISGEEEESVLLPELEENEVLKEESIEGKQHFTQPPARYSEAAFVKLLEEKGIGRPSTYVP
TISTLISRKYVDREKKILIPTELGFIVNDILSNYFKQIVDTDFTAEMEVKLDNVEAGKESWTHIVDEFFTPLKEDIEKAE
KEISKVIIEDKVSDVPCDKCGRLMVIKHGRFGDFLACPGYPECQNTKPIVEEVDANCPLCGGKILVKRSKKGNRFYGCSN
YPECNFVSWYEPTNEKCPECGSYMVKRYSKSKGEYLQCSDKECKYEKIIEKNNDENNSEK

Sequences:

>Translated_700_residues
MGQKLVIVESPAKAKTIEKYLGKNYVVEASMGHVRDLPKSQLGVDIENEYNPKYITIRGKGELLSKLRKLAKKSDKIYLA
TDPDREGEAISWHLANVLKIDENENCRIEFNEITKDAVKNSIKHPRKINCNLVDAQQARRVLDRLVGYEISPLLWRNVKW
GLSAGRVQSAALKLICDREEEIKKFNPEEYWTVDVKLKKGKKSFPVKLTTKNKKKIEIKNKEQADQIIDELKENEYIVSK
IKKGTKNKNPLAPFTTSTLQQEASKKLNFMTKKTMSVAQQLYEGVEVKKFGTVGLITYMRTDSVRISKEAQEKALNFIDE
TYGKEYVPEESRVYKGKKNIQDAHEAIRPTYVEITPEIAKANLSNDQYKLYALIWKRFIASQMATCILNTNSLEIKNGDY
TLRASGSTIKFDGFMKVYEYISGEEEESVLLPELEENEVLKEESIEGKQHFTQPPARYSEAAFVKLLEEKGIGRPSTYVP
TISTLISRKYVDREKKILIPTELGFIVNDILSNYFKQIVDTDFTAEMEVKLDNVEAGKESWTHIVDEFFTPLKEDIEKAE
KEISKVIIEDKVSDVPCDKCGRLMVIKHGRFGDFLACPGYPECQNTKPIVEEVDANCPLCGGKILVKRSKKGNRFYGCSN
YPECNFVSWYEPTNEKCPECGSYMVKRYSKSKGEYLQCSDKECKYEKIIEKNNDENNSEK
>Mature_699_residues
GQKLVIVESPAKAKTIEKYLGKNYVVEASMGHVRDLPKSQLGVDIENEYNPKYITIRGKGELLSKLRKLAKKSDKIYLAT
DPDREGEAISWHLANVLKIDENENCRIEFNEITKDAVKNSIKHPRKINCNLVDAQQARRVLDRLVGYEISPLLWRNVKWG
LSAGRVQSAALKLICDREEEIKKFNPEEYWTVDVKLKKGKKSFPVKLTTKNKKKIEIKNKEQADQIIDELKENEYIVSKI
KKGTKNKNPLAPFTTSTLQQEASKKLNFMTKKTMSVAQQLYEGVEVKKFGTVGLITYMRTDSVRISKEAQEKALNFIDET
YGKEYVPEESRVYKGKKNIQDAHEAIRPTYVEITPEIAKANLSNDQYKLYALIWKRFIASQMATCILNTNSLEIKNGDYT
LRASGSTIKFDGFMKVYEYISGEEEESVLLPELEENEVLKEESIEGKQHFTQPPARYSEAAFVKLLEEKGIGRPSTYVPT
ISTLISRKYVDREKKILIPTELGFIVNDILSNYFKQIVDTDFTAEMEVKLDNVEAGKESWTHIVDEFFTPLKEDIEKAEK
EISKVIIEDKVSDVPCDKCGRLMVIKHGRFGDFLACPGYPECQNTKPIVEEVDANCPLCGGKILVKRSKKGNRFYGCSNY
PECNFVSWYEPTNEKCPECGSYMVKRYSKSKGEYLQCSDKECKYEKIIEKNNDENNSEK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=631, Percent_Identity=23.7717908082409, Blast_Score=123, Evalue=7e-28,
Organism=Homo sapiens, GI4507635, Length=576, Percent_Identity=23.4375, Blast_Score=114, Evalue=4e-25,
Organism=Escherichia coli, GI1787529, Length=759, Percent_Identity=38.471673254282, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1788061, Length=543, Percent_Identity=20.8103130755064, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994931, Length=140, Percent_Identity=35, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32563869, Length=498, Percent_Identity=25.3012048192771, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=509, Percent_Identity=26.5225933202358, Blast_Score=129, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6323263, Length=555, Percent_Identity=24.6846846846847, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585251, Length=619, Percent_Identity=21.6478190630048, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 80227; Mature: 80096

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQKLVIVESPAKAKTIEKYLGKNYVVEASMGHVRDLPKSQLGVDIENEYNPKYITIRGK
CCCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCHHHHCCCCCCCCCCCEEEEECC
GELLSKLRKLAKKSDKIYLATDPDREGEAISWHLANVLKIDENENCRIEFNEITKDAVKN
HHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEEEEEECCCCCCEEEHHHHHHHHHHH
SIKHPRKINCNLVDAQQARRVLDRLVGYEISPLLWRNVKWGLSAGRVQSAALKLICDREE
HHCCCCCCCCEEECHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCHH
EIKKFNPEEYWTVDVKLKKGKKSFPVKLTTKNKKKIEIKNKEQADQIIDELKENEYIVSK
HHHHCCCCCEEEEEEEEECCCCCCCEEEECCCCCEEEECCHHHHHHHHHHHHCCHHHHHH
IKKGTKNKNPLAPFTTSTLQQEASKKLNFMTKKTMSVAQQLYEGVEVKKFGTVGLITYMR
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEEE
TDSVRISKEAQEKALNFIDETYGKEYVPEESRVYKGKKNIQDAHEAIRPTYVEITPEIAK
CCCEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHCCEEEEECHHHHH
ANLSNDQYKLYALIWKRFIASQMATCILNTNSLEIKNGDYTLRASGSTIKFDGFMKVYEY
CCCCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCEEEEECCCEEEEHHHHHHHHH
ISGEEEESVLLPELEENEVLKEESIEGKQHFTQPPARYSEAAFVKLLEEKGIGRPSTYVP
HCCCCCCCEECCCCCCCHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHH
TISTLISRKYVDREKKILIPTELGFIVNDILSNYFKQIVDTDFTAEMEVKLDNVEAGKES
HHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHH
WTHIVDEFFTPLKEDIEKAEKEISKVIIEDKVSDVPCDKCGRLMVIKHGRFGDFLACPGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEECCCCCCEEECCCC
PECQNTKPIVEEVDANCPLCGGKILVKRSKKGNRFYGCSNYPECNFVSWYEPTNEKCPEC
CCCCCCCCHHHHCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCEEEEECCCCCCCCHH
GSYMVKRYSKSKGEYLQCSDKECKYEKIIEKNNDENNSEK
HHHHHHHHHCCCCCEEECCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GQKLVIVESPAKAKTIEKYLGKNYVVEASMGHVRDLPKSQLGVDIENEYNPKYITIRGK
CCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCHHHHCCCCCCCCCCCEEEEECC
GELLSKLRKLAKKSDKIYLATDPDREGEAISWHLANVLKIDENENCRIEFNEITKDAVKN
HHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEEEEEECCCCCCEEEHHHHHHHHHHH
SIKHPRKINCNLVDAQQARRVLDRLVGYEISPLLWRNVKWGLSAGRVQSAALKLICDREE
HHCCCCCCCCEEECHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCHH
EIKKFNPEEYWTVDVKLKKGKKSFPVKLTTKNKKKIEIKNKEQADQIIDELKENEYIVSK
HHHHCCCCCEEEEEEEEECCCCCCCEEEECCCCCEEEECCHHHHHHHHHHHHCCHHHHHH
IKKGTKNKNPLAPFTTSTLQQEASKKLNFMTKKTMSVAQQLYEGVEVKKFGTVGLITYMR
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEEE
TDSVRISKEAQEKALNFIDETYGKEYVPEESRVYKGKKNIQDAHEAIRPTYVEITPEIAK
CCCEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHCCEEEEECHHHHH
ANLSNDQYKLYALIWKRFIASQMATCILNTNSLEIKNGDYTLRASGSTIKFDGFMKVYEY
CCCCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCEEEEECCCEEEEHHHHHHHHH
ISGEEEESVLLPELEENEVLKEESIEGKQHFTQPPARYSEAAFVKLLEEKGIGRPSTYVP
HCCCCCCCEECCCCCCCHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHH
TISTLISRKYVDREKKILIPTELGFIVNDILSNYFKQIVDTDFTAEMEVKLDNVEAGKES
HHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHH
WTHIVDEFFTPLKEDIEKAEKEISKVIIEDKVSDVPCDKCGRLMVIKHGRFGDFLACPGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEECCCCCCEEECCCC
PECQNTKPIVEEVDANCPLCGGKILVKRSKKGNRFYGCSNYPECNFVSWYEPTNEKCPEC
CCCCCCCCHHHHCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCEEEEECCCCCCCCHH
GSYMVKRYSKSKGEYLQCSDKECKYEKIIEKNNDENNSEK
HHHHHHHHHCCCCCEEECCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]