Click here to switch to the map view.

The map label for this gene is ispG

Identifier: 18310674

GI number: 18310674

Start: 1970830

End: 1971879

Strand: Reverse

Name: ispG

Synonym: CPE1692

Alternate gene names: 18310674

Gene position: 1971879-1970830 (Counterclockwise)

Preceding gene: 18310675

Following gene: 18310673

Centisome position: 65.05

GC content: 31.71

Gene sequence:

>1050_bases
GTGAATAGAAAAGAAACTAGAAAGGTTAAGGTTGGAAACATATATGTTGGAGGAGACTTTAGAGTTTCCATTCAATCTAT
GACAAATACAGATACAAAAGATGTAGAGTCTACAGTTAAACAAATAAAAGAGCTTCAAGAAGCTGGATGTGATATTGTTA
GATGTGCAGTTTTAGATATGGATGCAGCTTGCGCTATAAAAGATATAGTGGCAAAAATTAATATACCACTAGTTGCAGAT
ATTCATTTTGATTATAGATTAGCTTTAAAAGCAATAGAAAATGGAGTTTCTGCAATAAGAATAAATCCAGGAAATATTGG
ATCTAGAGAAAAGGTAGAAGCTGTAGTAAAAGCTTGTAAAGAAAAAAATATTCCTATAAGAATAGGGGTTAACTCAGGGT
CATTATCAAAAGAACTTTTAACAAAATATGGAAAACCTACCCCGGATGCCCTAGTTGAAAGTGCATTAGAACATGTTAAA
ATATTAGAAGAGTTAGATTTTCATGATATAGTAATTTCAATGAAATCATCAAATGTTGAAACTATGATAGAAAGTTATAG
AATAGCTTCACAAAAAACAAATTATCCTCTTCACTTAGGGGTTACTGAGGCTGGTACACCTTGGAGAGGAACAATAAAAT
CTGCTATAGGAATAGGAACTTTACTTGCAGAAGGAATAGGTGATACTATAAGAGTTTCTTTAACTGGAGATCCTGTTGAA
GAGATAAAAGTAGGTAAAGAAATTCTTAAAAACTTTGGATATGTAAAAGAAGGAATAGAGTTTATATCATGTCCTACATG
TGGAAGAACTCAAATAGACTTAATAAACATAGCTAAAGAAGTAGAAGAAAGATTAAGTTCTTGCAAGAAAAACATAAAGG
TTGCAGTAATGGGCTGTGTTGTAAATGGACCAGGAGAAGCGAGAGAGGCAGATATTGGAATAGCTGGAGGTAAAGGTGAA
GGTCTTATCTTTAGAAAAGGTGAAATAATTAAAAAAGTAAAAGAAGAAGACTTAGTTGAAGAGCTTATAAAGATAATAGA
AACAATATAA

Upstream 100 bases:

>100_bases
GTAAATACCGTTGGATTCATGCTCCTTATGGGACTTATGGTATTAGTAACTATAAAAGATATAATATTCCCGATACTATA
GAAATTTGGAGGAATTAATA

Downstream 100 bases:

>100_bases
TTTAAAGGAGGGATTAATATCCCTTCTTTATGTCGTATAGGGATAAATTAAAATAAAATTCAAAGGAGGCTTAGATTTTG
AGTAACGAGTTTGTTAAACA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MNRKETRKVKVGNIYVGGDFRVSIQSMTNTDTKDVESTVKQIKELQEAGCDIVRCAVLDMDAACAIKDIVAKINIPLVAD
IHFDYRLALKAIENGVSAIRINPGNIGSREKVEAVVKACKEKNIPIRIGVNSGSLSKELLTKYGKPTPDALVESALEHVK
ILEELDFHDIVISMKSSNVETMIESYRIASQKTNYPLHLGVTEAGTPWRGTIKSAIGIGTLLAEGIGDTIRVSLTGDPVE
EIKVGKEILKNFGYVKEGIEFISCPTCGRTQIDLINIAKEVEERLSSCKKNIKVAVMGCVVNGPGEAREADIGIAGGKGE
GLIFRKGEIIKKVKEEDLVEELIKIIETI

Sequences:

>Translated_349_residues
MNRKETRKVKVGNIYVGGDFRVSIQSMTNTDTKDVESTVKQIKELQEAGCDIVRCAVLDMDAACAIKDIVAKINIPLVAD
IHFDYRLALKAIENGVSAIRINPGNIGSREKVEAVVKACKEKNIPIRIGVNSGSLSKELLTKYGKPTPDALVESALEHVK
ILEELDFHDIVISMKSSNVETMIESYRIASQKTNYPLHLGVTEAGTPWRGTIKSAIGIGTLLAEGIGDTIRVSLTGDPVE
EIKVGKEILKNFGYVKEGIEFISCPTCGRTQIDLINIAKEVEERLSSCKKNIKVAVMGCVVNGPGEAREADIGIAGGKGE
GLIFRKGEIIKKVKEEDLVEELIKIIETI
>Mature_349_residues
MNRKETRKVKVGNIYVGGDFRVSIQSMTNTDTKDVESTVKQIKELQEAGCDIVRCAVLDMDAACAIKDIVAKINIPLVAD
IHFDYRLALKAIENGVSAIRINPGNIGSREKVEAVVKACKEKNIPIRIGVNSGSLSKELLTKYGKPTPDALVESALEHVK
ILEELDFHDIVISMKSSNVETMIESYRIASQKTNYPLHLGVTEAGTPWRGTIKSAIGIGTLLAEGIGDTIRVSLTGDPVE
EIKVGKEILKNFGYVKEGIEFISCPTCGRTQIDLINIAKEVEERLSSCKKNIKVAVMGCVVNGPGEAREADIGIAGGKGE
GLIFRKGEIIKKVKEEDLVEELIKIIETI

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=342, Percent_Identity=50.5847953216374, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_CLOPE (P58667)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_562608.1
- ProteinModelPortal:   P58667
- GeneID:   990002
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE1692
- NMPDR:   fig|195102.1.peg.1755
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   P58667
- ProtClustDB:   PRK00366
- BioCyc:   CPER195102:CPE1692-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 38018; Mature: 38018

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKETRKVKVGNIYVGGDFRVSIQSMTNTDTKDVESTVKQIKELQEAGCDIVRCAVLDM
CCCCCCCEEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECC
DAACAIKDIVAKINIPLVADIHFDYRLALKAIENGVSAIRINPGNIGSREKVEAVVKACK
CHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHH
EKNIPIRIGVNSGSLSKELLTKYGKPTPDALVESALEHVKILEELDFHDIVISMKSSNVE
HCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
TMIESYRIASQKTNYPLHLGVTEAGTPWRGTIKSAIGIGTLLAEGIGDTIRVSLTGDPVE
HHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
EIKVGKEILKNFGYVKEGIEFISCPTCGRTQIDLINIAKEVEERLSSCKKNIKVAVMGCV
HHHHHHHHHHHCCHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
VNGPGEAREADIGIAGGKGEGLIFRKGEIIKKVKEEDLVEELIKIIETI
ECCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRKETRKVKVGNIYVGGDFRVSIQSMTNTDTKDVESTVKQIKELQEAGCDIVRCAVLDM
CCCCCCCEEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECC
DAACAIKDIVAKINIPLVADIHFDYRLALKAIENGVSAIRINPGNIGSREKVEAVVKACK
CHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHH
EKNIPIRIGVNSGSLSKELLTKYGKPTPDALVESALEHVKILEELDFHDIVISMKSSNVE
HCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
TMIESYRIASQKTNYPLHLGVTEAGTPWRGTIKSAIGIGTLLAEGIGDTIRVSLTGDPVE
HHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
EIKVGKEILKNFGYVKEGIEFISCPTCGRTQIDLINIAKEVEERLSSCKKNIKVAVMGCV
HHHHHHHHHHHCCHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
VNGPGEAREADIGIAGGKGEGLIFRKGEIIKKVKEEDLVEELIKIIETI
ECCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842