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The map label for this gene is moeZ [H]

Identifier: 18310584

GI number: 18310584

Start: 1875061

End: 1875870

Strand: Reverse

Name: moeZ [H]

Synonym: CPE1602

Alternate gene names: 18310584

Gene position: 1875870-1875061 (Counterclockwise)

Preceding gene: 18310585

Following gene: 18310583

Centisome position: 61.88

GC content: 28.89

Gene sequence:

>810_bases
ATGAATATAAAGATTAATGAAAAGTGGAGAGAGGTAAAAGAAAATTGTACAGTATATGCTTTAAAAAATGAAGAATTTCC
TGATTCTCATGTTATAGTTTTAAATGGATTTCCACTTGTAGAAGATAAGAAACTTAAGGATGGAGATAGGATTGTCTTTA
TAAAAAAGGGAATTATGCCTAATAAAGAGGAGTTAGAAGAACTAATGGTATCTAGGCATACACCAGGTATTCATGAAAAA
CTAAAAAGAATTAAGGTTTTAATAGCTGGATGTGGAGGCCTTGGATCTAATATTGCTATTTCCTTAGGAAGAATAGGGGT
TGGAAGTATAAGCTTAGTAGACTTTGATATAGTAGAACCTTCTAATTTAAATAGGCAACAGTATTATATTGAGGATATTG
GAGAATTTAAAGTAACTGCTTTAAAAAGAAACTTAATTAGAATAAATCCTTTTATAAAAATAGAAGCTTTAAATCTTAAG
CTTTCTGAAGATAATATGGGTATTTTACAAGGCGCTGACATAATAATAGAAGCCTTTGATAATCCAGATTATAAAGCCTT
ATTAGCTAATTATGTTCTAGGTAATATGAAGGACAAATTTTTGATTTCTTCTTCAGGAATGGCTGGCTTTTATGATTCAA
ATTTAATAAAAACAAAGCAAATAACAGATAAGTTTTATATTTGTGGAGATTTAGTAAATGAAGCTAAGGAAGGCTGTGGA
TTAATGGCTCCTAGAGTGGCTATATGTGCAAATCACATGGCTAATTTAGTTATAGATATAGCTATTAATAAAATAGAATT
TCAAAAATAA

Upstream 100 bases:

>100_bases
TATTGCCTTTGTTGGTGGGGGATGATTAAGAAGTATTTAAGAAGTCTAAACAATAAAAGGTGAGTGAAAATAAAAGAGAA
AAAAGATAAGAGGGACCTTT

Downstream 100 bases:

>100_bases
ATAATAGTAAAAATAGAGTGGATTTTAAGTGCTTTTTAGACAGAGTTTATATATAAAAAATAAAATACTAGTCTAAAATA
TGAAGAAATAAGAAGAGTAG

Product: thiamine biosynthesis protein ThiF

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNIKINEKWREVKENCTVYALKNEEFPDSHVIVLNGFPLVEDKKLKDGDRIVFIKKGIMPNKEELEELMVSRHTPGIHEK
LKRIKVLIAGCGGLGSNIAISLGRIGVGSISLVDFDIVEPSNLNRQQYYIEDIGEFKVTALKRNLIRINPFIKIEALNLK
LSEDNMGILQGADIIIEAFDNPDYKALLANYVLGNMKDKFLISSSGMAGFYDSNLIKTKQITDKFYICGDLVNEAKEGCG
LMAPRVAICANHMANLVIDIAINKIEFQK

Sequences:

>Translated_269_residues
MNIKINEKWREVKENCTVYALKNEEFPDSHVIVLNGFPLVEDKKLKDGDRIVFIKKGIMPNKEELEELMVSRHTPGIHEK
LKRIKVLIAGCGGLGSNIAISLGRIGVGSISLVDFDIVEPSNLNRQQYYIEDIGEFKVTALKRNLIRINPFIKIEALNLK
LSEDNMGILQGADIIIEAFDNPDYKALLANYVLGNMKDKFLISSSGMAGFYDSNLIKTKQITDKFYICGDLVNEAKEGCG
LMAPRVAICANHMANLVIDIAINKIEFQK
>Mature_269_residues
MNIKINEKWREVKENCTVYALKNEEFPDSHVIVLNGFPLVEDKKLKDGDRIVFIKKGIMPNKEELEELMVSRHTPGIHEK
LKRIKVLIAGCGGLGSNIAISLGRIGVGSISLVDFDIVEPSNLNRQQYYIEDIGEFKVTALKRNLIRINPFIKIEALNLK
LSEDNMGILQGADIIIEAFDNPDYKALLANYVLGNMKDKFLISSSGMAGFYDSNLIKTKQITDKFYICGDLVNEAKEGCG
LMAPRVAICANHMANLVIDIAINKIEFQK

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1787048, Length=106, Percent_Identity=38.6792452830189, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789177, Length=141, Percent_Identity=31.2056737588652, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082356, Length=106, Percent_Identity=36.7924528301887, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=207, Percent_Identity=30.4347826086957, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=126, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582879, Length=130, Percent_Identity=36.9230769230769, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 30147; Mature: 30147

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKINEKWREVKENCTVYALKNEEFPDSHVIVLNGFPLVEDKKLKDGDRIVFIKKGIMP
CCEEECHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCC
NKEELEELMVSRHTPGIHEKLKRIKVLIAGCGGLGSNIAISLGRIGVGSISLVDFDIVEP
CHHHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCCCCEEEEECCCCCCEEEEEEEEEECC
SNLNRQQYYIEDIGEFKVTALKRNLIRINPFIKIEALNLKLSEDNMGILQGADIIIEAFD
CCCCCCEEEHHHCCCEEEEEECCCEEEECCEEEEEEEEEEECCCCCCEEECCEEEEEECC
NPDYKALLANYVLGNMKDKFLISSSGMAGFYDSNLIKTKQITDKFYICGDLVNEAKEGCG
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEHECCCEEEEEHHHHHHHHHCCC
LMAPRVAICANHMANLVIDIAINKIEFQK
CCCCHHHHHHHHHHHEEEEEEEEEEEECC
>Mature Secondary Structure
MNIKINEKWREVKENCTVYALKNEEFPDSHVIVLNGFPLVEDKKLKDGDRIVFIKKGIMP
CCEEECHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCC
NKEELEELMVSRHTPGIHEKLKRIKVLIAGCGGLGSNIAISLGRIGVGSISLVDFDIVEP
CHHHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCCCCEEEEECCCCCCEEEEEEEEEECC
SNLNRQQYYIEDIGEFKVTALKRNLIRINPFIKIEALNLKLSEDNMGILQGADIIIEAFD
CCCCCCEEEHHHCCCEEEEEECCCEEEECCEEEEEEEEEEECCCCCCEEECCEEEEEECC
NPDYKALLANYVLGNMKDKFLISSSGMAGFYDSNLIKTKQITDKFYICGDLVNEAKEGCG
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEHECCCEEEEEHHHHHHHHHCCC
LMAPRVAICANHMANLVIDIAINKIEFQK
CCCCHHHHHHHHHHHEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]