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The map label for this gene is gdhA [H]

Identifier: 18310500

GI number: 18310500

Start: 1775558

End: 1776904

Strand: Reverse

Name: gdhA [H]

Synonym: CPE1518

Alternate gene names: 18310500

Gene position: 1776904-1775558 (Counterclockwise)

Preceding gene: 18310501

Following gene: 18310499

Centisome position: 58.62

GC content: 32.74

Gene sequence:

>1347_bases
ATGGAAGTAAAAAAATATGTAGATAATTTAATGGAAGATTTAAAGAAAAATAACCCTGGAGAAAGTGAGTTCTTAGCTGC
TGCAGAAGAAGTGTTATACTCTTTAGTACCTGTCTTAGAAAAGAATCCTAAGTATATGGAAGAGGGAATATTAGAAAGAA
TTGTTGAACCAGAGAGAGTTATAATGTTTAGAGTTCCTTGGGTTGACGATGCTGGAAATGTAAGAGTTAACAGAGGATAT
AGAGTACAATTTAATAGTGCTATAGGACCATATAAGGGAGGATTAAGATTCCATCCTTCAGTTAATTTAAGCATAATAAA
ATTTTTAGGATTTGAGCAAATATTTAAGAATTCTTTAACTACTCTTCCTATTGGAGGAGGAAAAGGAGGATCTAACTTTG
ATCCTAAGGGAAAATCAGATAGAGAGATAATGAGATTTTGCCAAAGCTTTATGAGTGAACTTTATAGACATATAGGACCT
AATACAGATGTTCCTGCAGGAGATATTGGGGTTGGAGGAAGAGAAATAGGCTATATGTTTGGTCAATATAAAAAACTTAA
AAATTCAGTAGATGCTGGAGTTTTAACTGGCAAGGGCTTAACTTATGGTGGTAGCTTAGCTAGAAAAGAAGCAACTGGAT
ATGGTTTAGTATATTTTGTAGATGAAATGTTAAGAGATAACGGTCAAACAATAGAAGGAAAAACTGTTGTAATCTCAGGT
TCAGGAAATGTTGCAATATATGCCACTGAAAAAGTTCAAGAGCTTGGAGGAAAGGTTGTTGCACTTTCAGATTCATCAGG
GTATGTATATGATGAAAATGGAATAGATTTAGAGGTTGTAAAAGAAATCAAAGAAGTAAAAAGAGGAAGAATTTCTGAAT
ATGTAAATTATGTTAAAACTGCTAAGTTTACTGAGGGATTCAGAGGAATTTGGAATGTTAAATGTGATATAGCTCTTCCT
TGTGCTACTCAAAATGAAATAGATAAAAGCAGTGCAAAAACTTTAATTGATAATGGAGTAATTGCTGTAGGAGAAGGAGC
AAATATGCCTTCAACTTTAGAAGCTCAAAAACTTTTTGTGGATAATAAAATTTTATTTGCTCCAGCAAAAGCTGCCAATG
CAGGTGGAGTAGCAACATCAGCTTTAGAAATGAGTCAAAATAGTTTAAGAATGTCTTGGACTTTTGAAGAGGTTGATGCA
AAACTTAAAGACATAATGAAAAATATATACTATAATTCTAGAAATGCAGCTTCTGAATATGGTCATGATGGAAATCTAAT
AGTTGGAGCTAATATTGCAGGATTTAAAAAGGTTGCAGATGCTATGTTAGACCATGGTATTATATAA

Upstream 100 bases:

>100_bases
TTAAGAAAATATTTTCCTAGAATATTAGCTTAAAGCTATAACTTTTAAATAATATAGTCAATGTAAAAACTATAGTGAAT
ATAAAAAAGGGGGCATTAAA

Downstream 100 bases:

>100_bases
GAAATACTTTTAAAATAGTTGTTATTTTAAGTATTATATATTTATAAAGTTTGATATTTAAATTAAAGCTAAACTTTATA
GAATAATAAAATAGAGAATA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MEVKKYVDNLMEDLKKNNPGESEFLAAAEEVLYSLVPVLEKNPKYMEEGILERIVEPERVIMFRVPWVDDAGNVRVNRGY
RVQFNSAIGPYKGGLRFHPSVNLSIIKFLGFEQIFKNSLTTLPIGGGKGGSNFDPKGKSDREIMRFCQSFMSELYRHIGP
NTDVPAGDIGVGGREIGYMFGQYKKLKNSVDAGVLTGKGLTYGGSLARKEATGYGLVYFVDEMLRDNGQTIEGKTVVISG
SGNVAIYATEKVQELGGKVVALSDSSGYVYDENGIDLEVVKEIKEVKRGRISEYVNYVKTAKFTEGFRGIWNVKCDIALP
CATQNEIDKSSAKTLIDNGVIAVGEGANMPSTLEAQKLFVDNKILFAPAKAANAGGVATSALEMSQNSLRMSWTFEEVDA
KLKDIMKNIYYNSRNAASEYGHDGNLIVGANIAGFKKVADAMLDHGII

Sequences:

>Translated_448_residues
MEVKKYVDNLMEDLKKNNPGESEFLAAAEEVLYSLVPVLEKNPKYMEEGILERIVEPERVIMFRVPWVDDAGNVRVNRGY
RVQFNSAIGPYKGGLRFHPSVNLSIIKFLGFEQIFKNSLTTLPIGGGKGGSNFDPKGKSDREIMRFCQSFMSELYRHIGP
NTDVPAGDIGVGGREIGYMFGQYKKLKNSVDAGVLTGKGLTYGGSLARKEATGYGLVYFVDEMLRDNGQTIEGKTVVISG
SGNVAIYATEKVQELGGKVVALSDSSGYVYDENGIDLEVVKEIKEVKRGRISEYVNYVKTAKFTEGFRGIWNVKCDIALP
CATQNEIDKSSAKTLIDNGVIAVGEGANMPSTLEAQKLFVDNKILFAPAKAANAGGVATSALEMSQNSLRMSWTFEEVDA
KLKDIMKNIYYNSRNAASEYGHDGNLIVGANIAGFKKVADAMLDHGII
>Mature_448_residues
MEVKKYVDNLMEDLKKNNPGESEFLAAAEEVLYSLVPVLEKNPKYMEEGILERIVEPERVIMFRVPWVDDAGNVRVNRGY
RVQFNSAIGPYKGGLRFHPSVNLSIIKFLGFEQIFKNSLTTLPIGGGKGGSNFDPKGKSDREIMRFCQSFMSELYRHIGP
NTDVPAGDIGVGGREIGYMFGQYKKLKNSVDAGVLTGKGLTYGGSLARKEATGYGLVYFVDEMLRDNGQTIEGKTVVISG
SGNVAIYATEKVQELGGKVVALSDSSGYVYDENGIDLEVVKEIKEVKRGRISEYVNYVKTAKFTEGFRGIWNVKCDIALP
CATQNEIDKSSAKTLIDNGVIAVGEGANMPSTLEAQKLFVDNKILFAPAKAANAGGVATSALEMSQNSLRMSWTFEEVDA
KLKDIMKNIYYNSRNAASEYGHDGNLIVGANIAGFKKVADAMLDHGII

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=359, Percent_Identity=32.5905292479109, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI4885281, Length=359, Percent_Identity=32.033426183844, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1788059, Length=453, Percent_Identity=58.2781456953642, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17544676, Length=368, Percent_Identity=29.8913043478261, Blast_Score=144, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=54.1850220264317, Blast_Score=415, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=53.9823008849557, Blast_Score=413, Evalue=1e-116,
Organism=Drosophila melanogaster, GI45549226, Length=359, Percent_Identity=33.4261838440111, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI45553475, Length=359, Percent_Identity=33.4261838440111, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24649283, Length=420, Percent_Identity=30.2380952380952, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24649460, Length=292, Percent_Identity=33.5616438356164, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 49053; Mature: 49053

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKKYVDNLMEDLKKNNPGESEFLAAAEEVLYSLVPVLEKNPKYMEEGILERIVEPERV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
IMFRVPWVDDAGNVRVNRGYRVQFNSAIGPYKGGLRFHPSVNLSIIKFLGFEQIFKNSLT
EEEEECCCCCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHCCCC
TLPIGGGKGGSNFDPKGKSDREIMRFCQSFMSELYRHIGPNTDVPAGDIGVGGREIGYMF
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GQYKKLKNSVDAGVLTGKGLTYGGSLARKEATGYGLVYFVDEMLRDNGQTIEGKTVVISG
HHHHHHHHCCCCCEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEECCCEEEEEC
SGNVAIYATEKVQELGGKVVALSDSSGYVYDENGIDLEVVKEIKEVKRGRISEYVNYVKT
CCCEEEEECHHHHHHCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKFTEGFRGIWNVKCDIALPCATQNEIDKSSAKTLIDNGVIAVGEGANMPSTLEAQKLFV
HHHHHCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCHHHHHHEE
DNKILFAPAKAANAGGVATSALEMSQNSLRMSWTFEEVDAKLKDIMKNIYYNSRNAASEY
CCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHC
GHDGNLIVGANIAGFKKVADAMLDHGII
CCCCCEEEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEVKKYVDNLMEDLKKNNPGESEFLAAAEEVLYSLVPVLEKNPKYMEEGILERIVEPERV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
IMFRVPWVDDAGNVRVNRGYRVQFNSAIGPYKGGLRFHPSVNLSIIKFLGFEQIFKNSLT
EEEEECCCCCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHCCCC
TLPIGGGKGGSNFDPKGKSDREIMRFCQSFMSELYRHIGPNTDVPAGDIGVGGREIGYMF
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GQYKKLKNSVDAGVLTGKGLTYGGSLARKEATGYGLVYFVDEMLRDNGQTIEGKTVVISG
HHHHHHHHCCCCCEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEECCCEEEEEC
SGNVAIYATEKVQELGGKVVALSDSSGYVYDENGIDLEVVKEIKEVKRGRISEYVNYVKT
CCCEEEEECHHHHHHCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKFTEGFRGIWNVKCDIALPCATQNEIDKSSAKTLIDNGVIAVGEGANMPSTLEAQKLFV
HHHHHCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCHHHHHHEE
DNKILFAPAKAANAGGVATSALEMSQNSLRMSWTFEEVDAKLKDIMKNIYYNSRNAASEY
CCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHC
GHDGNLIVGANIAGFKKVADAMLDHGII
CCCCCEEEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]