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The map label for this gene is topB [H]

Identifier: 18310490

GI number: 18310490

Start: 1761676

End: 1763862

Strand: Reverse

Name: topB [H]

Synonym: CPE1508

Alternate gene names: 18310490

Gene position: 1763862-1761676 (Counterclockwise)

Preceding gene: 18310491

Following gene: 18310489

Centisome position: 58.19

GC content: 30.18

Gene sequence:

>2187_bases
TTGAAGAAATTAGTACTAGCAGAAAAACCTAGTGTAGGTAGAGAAATTGCCAAGGTTTTAAAATGCAATAATAATAAAGG
AAGTTATATTGAAGGAAAGGATTATGTAATAACTTGGGCTTTAGGCCATTTAGTAGAATTACAATCTCCAGAAGATTATG
ATAACAAGCTTAAAACATGGTCTATGGAAACTCTTCCAATGCTTCCTAAGCACATGAAACTTAAGGTTATTAAAAAAGCC
ACTAAGCAATTTTATGAAGTTAAAAAGCAAATGGAAAGAAAAGATATAGATGAAATAATAATAGCCACAGATGCTGGAAG
AGAAGGGGAGCTCGTAGCACGTTGGATAATAGAAAAAGCTCATGTTAAAAAGCCTATAAAGAGACTTTGGATATCCTCTC
AAACAGATAAAGCTATCTTAGATGGATTTAAAAATCTTAAACCAGGAGAAAATTACAATAACTTATATAAGGCTGCTCAG
TGTAGAGCTGAGGCTGACTGGTTAGTTGGTTTAAATGTTACTAGGGCCTTAACTTGTAAATATAATGCTCAGCTTTCAGC
AGGAAGGGTTCAATCACCTACCTTAGCAATGATAGTTCAAAGAGAAGAGGATATTAAAAACTTTAAACCAAAAGAGTATA
GTACTATTTTAATAGAAACAGATAAATGTAATTTTACATGGATAAATAAGGATAATAATTCGAGAATATTTAAAAATGAT
TTCAAAGAAAAAGTTGTTTCTAATTTAAATGAGAAGAAAAGTGGAAAAATAATCAATATAAATGAAAGTAATAAAAAGAA
ATTTTCTCCTCAATTATATGATTTAACTGAGCTTCAAAGAGATTGTAATAAAATTTTTGGATATTCAGCAAAACAGACCC
TTAATATAATGCAAAGATTATATGAAAATCATAAACTTCTTACATATCCAAGAACTGATTCAAGATATATTTCAAAGGAC
ATAGTTTCTACTATTCCAGATAGATTAAAAGCTGTAGCTACAGGAGAATTTAGAACTATAGCAAATGATTTATTAAAAAA
TCCTATAAAAGCAAACAAAAGTTTTGTAGATGACAATAAGGTTTCTGATCACCATGCTATAATTCCAACGGAGGAAAAAG
GAAATCTAGCTAATTTAAGTTCTGATGAGAGAAGAGTTTATGAGCTTGTAGTTAAGAGATTTCTAAGTGTATTAATGCCT
CCATTTGAATATATTCAAACAACTTTAACTGGTGAAGTTAATGGAGAAAAATTAATTGCAAAGGGAAAAGTAGTTAAAGC
TAAAGGATGGAAGAAGCTTTATGATAGAGAAGTTGATGATGAGGGAGAAGAGGATATAAAAGAACAAGAACTTCCTAAAT
TAAAAGAGGGAGATGAATTTAAAGTTCTTAAGGTTAATGTTAAAAAAGGAGAAACAAAGCCACCAGCAAGATTTAATGAG
GGAACTTTATTATCTGCTATGGAAAATCCTCAAAAATTTATATCAGTAGATAAATCCTCAGCAAAGACTTTAGGAGAAAC
AGGAGGACTTGGTACTGTTGCAACTAGAGCTGATATTATAGAAAAATTATTTAATTCCTTTGTAATAGAAAAGAGAGGAA
AGGAAATAATTCCAACTTCTAAGGGAAAACAACTTATAGATTTAGTACCAAAGGATTTAAAGTCACCACTTTTAACAGCT
AATTGGGAAGAGATGTTAGAGAGAATAAGTAAAGGAAAAGGTGATTCTAAGAAATTTATAAAAGATATAAGAAATTATAC
TGTTGCTTTAGTTGAGGATGTTAAGCTTGGAGAAAGTAAATTTCATCATGATAACTTAACAGGGAAAAAATGTCCTCAGT
GTGGTAAGTATATGCTTGAGGTAAAAGGCAAAAATGGAACAATGAATGTTTGCCAAGATAGAGAGTGTGGATATAGAGAG
AATATATCTAGAATAACTAATGCTAGATGTCCTGAGTGTAAGAAAAAGCTAGAGCTTAGGGGACATGGAGAAGGTAAAAT
ATATGTTTGCCCGGGAGTAAATTGTAACTTTAGAGAAAAAGAATCTCAATTTAAAAAGAGATTTGAGAAAAATAACAAAA
CAAATAAAAGAGAAGTAAATAAAATAATGCAAAAGATGAAAAAAGAAGCAAATGAAGATGTAAACAATCCTTTTGCTGAT
CTTTTAAGTGGTCTTAAATTTGACTAA

Upstream 100 bases:

>100_bases
CTAATAAAAATATAGGATTTTATTTGTTAAGTAAATATATTTAAGGTAGTATATTATTAGAGATATTACATATATGTAGT
ATCATGAAAGGATGATTTTA

Downstream 100 bases:

>100_bases
TTAAATTTTAAAAACCATATCAAATCTACCTAATAAAATTTATAAAATCTTATAAAAATATATTTATTTAAGAGACATAA
GTTTTATTTTAGGGCTTCAT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 728; Mature: 728

Protein sequence:

>728_residues
MKKLVLAEKPSVGREIAKVLKCNNNKGSYIEGKDYVITWALGHLVELQSPEDYDNKLKTWSMETLPMLPKHMKLKVIKKA
TKQFYEVKKQMERKDIDEIIIATDAGREGELVARWIIEKAHVKKPIKRLWISSQTDKAILDGFKNLKPGENYNNLYKAAQ
CRAEADWLVGLNVTRALTCKYNAQLSAGRVQSPTLAMIVQREEDIKNFKPKEYSTILIETDKCNFTWINKDNNSRIFKND
FKEKVVSNLNEKKSGKIININESNKKKFSPQLYDLTELQRDCNKIFGYSAKQTLNIMQRLYENHKLLTYPRTDSRYISKD
IVSTIPDRLKAVATGEFRTIANDLLKNPIKANKSFVDDNKVSDHHAIIPTEEKGNLANLSSDERRVYELVVKRFLSVLMP
PFEYIQTTLTGEVNGEKLIAKGKVVKAKGWKKLYDREVDDEGEEDIKEQELPKLKEGDEFKVLKVNVKKGETKPPARFNE
GTLLSAMENPQKFISVDKSSAKTLGETGGLGTVATRADIIEKLFNSFVIEKRGKEIIPTSKGKQLIDLVPKDLKSPLLTA
NWEEMLERISKGKGDSKKFIKDIRNYTVALVEDVKLGESKFHHDNLTGKKCPQCGKYMLEVKGKNGTMNVCQDRECGYRE
NISRITNARCPECKKKLELRGHGEGKIYVCPGVNCNFREKESQFKKRFEKNNKTNKREVNKIMQKMKKEANEDVNNPFAD
LLSGLKFD

Sequences:

>Translated_728_residues
MKKLVLAEKPSVGREIAKVLKCNNNKGSYIEGKDYVITWALGHLVELQSPEDYDNKLKTWSMETLPMLPKHMKLKVIKKA
TKQFYEVKKQMERKDIDEIIIATDAGREGELVARWIIEKAHVKKPIKRLWISSQTDKAILDGFKNLKPGENYNNLYKAAQ
CRAEADWLVGLNVTRALTCKYNAQLSAGRVQSPTLAMIVQREEDIKNFKPKEYSTILIETDKCNFTWINKDNNSRIFKND
FKEKVVSNLNEKKSGKIININESNKKKFSPQLYDLTELQRDCNKIFGYSAKQTLNIMQRLYENHKLLTYPRTDSRYISKD
IVSTIPDRLKAVATGEFRTIANDLLKNPIKANKSFVDDNKVSDHHAIIPTEEKGNLANLSSDERRVYELVVKRFLSVLMP
PFEYIQTTLTGEVNGEKLIAKGKVVKAKGWKKLYDREVDDEGEEDIKEQELPKLKEGDEFKVLKVNVKKGETKPPARFNE
GTLLSAMENPQKFISVDKSSAKTLGETGGLGTVATRADIIEKLFNSFVIEKRGKEIIPTSKGKQLIDLVPKDLKSPLLTA
NWEEMLERISKGKGDSKKFIKDIRNYTVALVEDVKLGESKFHHDNLTGKKCPQCGKYMLEVKGKNGTMNVCQDRECGYRE
NISRITNARCPECKKKLELRGHGEGKIYVCPGVNCNFREKESQFKKRFEKNNKTNKREVNKIMQKMKKEANEDVNNPFAD
LLSGLKFD
>Mature_728_residues
MKKLVLAEKPSVGREIAKVLKCNNNKGSYIEGKDYVITWALGHLVELQSPEDYDNKLKTWSMETLPMLPKHMKLKVIKKA
TKQFYEVKKQMERKDIDEIIIATDAGREGELVARWIIEKAHVKKPIKRLWISSQTDKAILDGFKNLKPGENYNNLYKAAQ
CRAEADWLVGLNVTRALTCKYNAQLSAGRVQSPTLAMIVQREEDIKNFKPKEYSTILIETDKCNFTWINKDNNSRIFKND
FKEKVVSNLNEKKSGKIININESNKKKFSPQLYDLTELQRDCNKIFGYSAKQTLNIMQRLYENHKLLTYPRTDSRYISKD
IVSTIPDRLKAVATGEFRTIANDLLKNPIKANKSFVDDNKVSDHHAIIPTEEKGNLANLSSDERRVYELVVKRFLSVLMP
PFEYIQTTLTGEVNGEKLIAKGKVVKAKGWKKLYDREVDDEGEEDIKEQELPKLKEGDEFKVLKVNVKKGETKPPARFNE
GTLLSAMENPQKFISVDKSSAKTLGETGGLGTVATRADIIEKLFNSFVIEKRGKEIIPTSKGKQLIDLVPKDLKSPLLTA
NWEEMLERISKGKGDSKKFIKDIRNYTVALVEDVKLGESKFHHDNLTGKKCPQCGKYMLEVKGKNGTMNVCQDRECGYRE
NISRITNARCPECKKKLELRGHGEGKIYVCPGVNCNFREKESQFKKRFEKNNKTNKREVNKIMQKMKKEANEDVNNPFAD
LLSGLKFD

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=650, Percent_Identity=25.8461538461538, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI4507635, Length=724, Percent_Identity=23.8950276243094, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1788061, Length=621, Percent_Identity=32.5281803542673, Blast_Score=316, Evalue=3e-87,
Organism=Escherichia coli, GI1787529, Length=673, Percent_Identity=24.81426448737, Blast_Score=156, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17555378, Length=638, Percent_Identity=24.7648902821317, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32563869, Length=686, Percent_Identity=24.198250728863, Blast_Score=135, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6323263, Length=664, Percent_Identity=24.6987951807229, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24640096, Length=681, Percent_Identity=23.201174743025, Blast_Score=133, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24585251, Length=623, Percent_Identity=25.2006420545746, Blast_Score=115, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 83341; Mature: 83341

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVLAEKPSVGREIAKVLKCNNNKGSYIEGKDYVITWALGHLVELQSPEDYDNKLKTW
CCCCEECCCCCHHHHHHHHHHCCCCCCCEECCCCEEEEEEHHHHHCCCCCCCCHHHHHHC
SMETLPMLPKHMKLKVIKKATKQFYEVKKQMERKDIDEIIIATDAGREGELVARWIIEKA
CCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
HVKKPIKRLWISSQTDKAILDGFKNLKPGENYNNLYKAAQCRAEADWLVGLNVTRALTCK
HHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCEEEEEEEE
YNAQLSAGRVQSPTLAMIVQREEDIKNFKPKEYSTILIETDKCNFTWINKDNNSRIFKND
ECCCCCCCCCCCCHHEEEEECHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHH
FKEKVVSNLNEKKSGKIININESNKKKFSPQLYDLTELQRDCNKIFGYSAKQTLNIMQRL
HHHHHHHCCCHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
YENHKLLTYPRTDSRYISKDIVSTIPDRLKAVATGEFRTIANDLLKNPIKANKSFVDDNK
HHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHCCCCCCCCCC
VSDHHAIIPTEEKGNLANLSSDERRVYELVVKRFLSVLMPPFEYIQTTLTGEVNGEKLIA
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEE
KGKVVKAKGWKKLYDREVDDEGEEDIKEQELPKLKEGDEFKVLKVNVKKGETKPPARFNE
CCCEEECCCHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
GTLLSAMENPQKFISVDKSSAKTLGETGGLGTVATRADIIEKLFNSFVIEKRGKEIIPTS
CCEEHHHCCCHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KGKQLIDLVPKDLKSPLLTANWEEMLERISKGKGDSKKFIKDIRNYTVALVEDVKLGESK
CCCHHHHHCCHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FHHDNLTGKKCPQCGKYMLEVKGKNGTMNVCQDRECGYRENISRITNARCPECKKKLELR
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCC
GHGEGKIYVCPGVNCNFREKESQFKKRFEKNNKTNKREVNKIMQKMKKEANEDVNNPFAD
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHH
LLSGLKFD
HHCCCCCC
>Mature Secondary Structure
MKKLVLAEKPSVGREIAKVLKCNNNKGSYIEGKDYVITWALGHLVELQSPEDYDNKLKTW
CCCCEECCCCCHHHHHHHHHHCCCCCCCEECCCCEEEEEEHHHHHCCCCCCCCHHHHHHC
SMETLPMLPKHMKLKVIKKATKQFYEVKKQMERKDIDEIIIATDAGREGELVARWIIEKA
CCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
HVKKPIKRLWISSQTDKAILDGFKNLKPGENYNNLYKAAQCRAEADWLVGLNVTRALTCK
HHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCEEEEEEEE
YNAQLSAGRVQSPTLAMIVQREEDIKNFKPKEYSTILIETDKCNFTWINKDNNSRIFKND
ECCCCCCCCCCCCHHEEEEECHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHH
FKEKVVSNLNEKKSGKIININESNKKKFSPQLYDLTELQRDCNKIFGYSAKQTLNIMQRL
HHHHHHHCCCHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
YENHKLLTYPRTDSRYISKDIVSTIPDRLKAVATGEFRTIANDLLKNPIKANKSFVDDNK
HHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHCCCCCCCCCC
VSDHHAIIPTEEKGNLANLSSDERRVYELVVKRFLSVLMPPFEYIQTTLTGEVNGEKLIA
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEE
KGKVVKAKGWKKLYDREVDDEGEEDIKEQELPKLKEGDEFKVLKVNVKKGETKPPARFNE
CCCEEECCCHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
GTLLSAMENPQKFISVDKSSAKTLGETGGLGTVATRADIIEKLFNSFVIEKRGKEIIPTS
CCEEHHHCCCHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KGKQLIDLVPKDLKSPLLTANWEEMLERISKGKGDSKKFIKDIRNYTVALVEDVKLGESK
CCCHHHHHCCHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FHHDNLTGKKCPQCGKYMLEVKGKNGTMNVCQDRECGYRENISRITNARCPECKKKLELR
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCC
GHGEGKIYVCPGVNCNFREKESQFKKRFEKNNKTNKREVNKIMQKMKKEANEDVNNPFAD
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHH
LLSGLKFD
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA