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The map label for this gene is comEC [H]

Identifier: 18310481

GI number: 18310481

Start: 1750939

End: 1751814

Strand: Reverse

Name: comEC [H]

Synonym: CPE1499

Alternate gene names: 18310481

Gene position: 1751814-1750939 (Counterclockwise)

Preceding gene: 18310482

Following gene: 18310480

Centisome position: 57.79

GC content: 30.94

Gene sequence:

>876_bases
ATGTTTAAAAAAATATTAGCAATAATAAGTACAGTTACAGTTTTAAGCTTTGCAGGATGTGGAGCTCAGAAAAATAATAT
TAGTAAAAGTTCTGCAGAGAGTGAATATAGTGGAATGAGTGTTACATATTTAAATGTTGGACAAGGTGATAGTGAGTTAA
TTCAGGTAAATGGAATTAATATGTTAATTGATGCAGGAACTAATGCAGGAGCTAATGACTTAGTTAAAGATTTAAAAAAT
AGAGGCATAAAAACTATAGATATAGCCATAGCAACTCATCCTCATGAAGATCATATAGGGGGAATGGATGAAGTTCTAGA
AAACTTTGATGTTAAATCTTTTTATGCTCCTAAGGTAGCACATACAACAAAAACTTATGAAAATATGTTAAAGGCAGTAA
AAAATGAGGGATTAAAAATAAAGCAGATAAAAGAAGGAACAAAGATTGATTTAGGAAAGGATACAGATGTTCAGGTTTAT
TCACCAGTTAAATCGCAGTATGAAGAACTAAATAACTATTCACCAGTAATGAAAATATCCTATGGACAAAATTCCTTTAT
GTTTACTGGAGATGCAGAAAGCTTAGTAGAAAAAGAAATTTTAAATGAAAATAAGGACTTAAAGGCCGATGTGCTTAAGT
TAGGACACCATGGATCACACTCATCAACTAGTGAGGAGTTTTTAAAAGCTGTTGATCCAAGCATAGCCATAGTTTCTTGC
GCTAAAGACAATAAATATGGTCATCCTCATAAAGAGACTATGAGCAATCTTAAAAAAGCTGGCATAACAGTTTATGAAAC
TTTCAGAGATGGTGATATAACAATAAGTTCTAATGGTAAAAAGTTAGATGTAAAATTACATAGCGAAAATAAATAA

Upstream 100 bases:

>100_bases
TATTAATTTTAGAAGGGGTATATTTCCTATAAAAATATTTTAAATTACAAAAAAATTAAAAATGTGTTAATATTATAAAA
AAAGGATTCTTGGAGGGAAA

Downstream 100 bases:

>100_bases
TAAAAGCTAAATTATAAAATCTTACATTGATTTTTAAGAATATATATTATATAATGACTAGGAAAAATAATATATAGACA
GTTCGAAAGCCTCCTGTCTT

Product: late competence protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MFKKILAIISTVTVLSFAGCGAQKNNISKSSAESEYSGMSVTYLNVGQGDSELIQVNGINMLIDAGTNAGANDLVKDLKN
RGIKTIDIAIATHPHEDHIGGMDEVLENFDVKSFYAPKVAHTTKTYENMLKAVKNEGLKIKQIKEGTKIDLGKDTDVQVY
SPVKSQYEELNNYSPVMKISYGQNSFMFTGDAESLVEKEILNENKDLKADVLKLGHHGSHSSTSEEFLKAVDPSIAIVSC
AKDNKYGHPHKETMSNLKKAGITVYETFRDGDITISSNGKKLDVKLHSENK

Sequences:

>Translated_291_residues
MFKKILAIISTVTVLSFAGCGAQKNNISKSSAESEYSGMSVTYLNVGQGDSELIQVNGINMLIDAGTNAGANDLVKDLKN
RGIKTIDIAIATHPHEDHIGGMDEVLENFDVKSFYAPKVAHTTKTYENMLKAVKNEGLKIKQIKEGTKIDLGKDTDVQVY
SPVKSQYEELNNYSPVMKISYGQNSFMFTGDAESLVEKEILNENKDLKADVLKLGHHGSHSSTSEEFLKAVDPSIAIVSC
AKDNKYGHPHKETMSNLKKAGITVYETFRDGDITISSNGKKLDVKLHSENK
>Mature_291_residues
MFKKILAIISTVTVLSFAGCGAQKNNISKSSAESEYSGMSVTYLNVGQGDSELIQVNGINMLIDAGTNAGANDLVKDLKN
RGIKTIDIAIATHPHEDHIGGMDEVLENFDVKSFYAPKVAHTTKTYENMLKAVKNEGLKIKQIKEGTKIDLGKDTDVQVY
SPVKSQYEELNNYSPVMKISYGQNSFMFTGDAESLVEKEILNENKDLKADVLKLGHHGSHSSTSEEFLKAVDPSIAIVSC
AKDNKYGHPHKETMSNLKKAGITVYETFRDGDITISSNGKKLDVKLHSENK

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG2333

COG function: function code R; Predicted hydrolase (metallo-beta-lactamase superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=248, Percent_Identity=24.1935483870968, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 31893; Mature: 31893

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKILAIISTVTVLSFAGCGAQKNNISKSSAESEYSGMSVTYLNVGQGDSELIQVNGIN
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCCEEEECCCE
MLIDAGTNAGANDLVKDLKNRGIKTIDIAIATHPHEDHIGGMDEVLENFDVKSFYAPKVA
EEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCHHHCCCHHHHHHCCCCCHHCCCHHH
HTTKTYENMLKAVKNEGLKIKQIKEGTKIDLGKDTDVQVYSPVKSQYEELNNYSPVMKIS
HHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCEEEECHHHHHHHHHCCCCCEEEEE
YGQNSFMFTGDAESLVEKEILNENKDLKADVLKLGHHGSHSSTSEEFLKAVDPSIAIVSC
ECCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEE
AKDNKYGHPHKETMSNLKKAGITVYETFRDGDITISSNGKKLDVKLHSENK
CCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCEEECCCCCEEEEEEECCCC
>Mature Secondary Structure
MFKKILAIISTVTVLSFAGCGAQKNNISKSSAESEYSGMSVTYLNVGQGDSELIQVNGIN
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCCEEEECCCE
MLIDAGTNAGANDLVKDLKNRGIKTIDIAIATHPHEDHIGGMDEVLENFDVKSFYAPKVA
EEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCHHHCCCHHHHHHCCCCCHHCCCHHH
HTTKTYENMLKAVKNEGLKIKQIKEGTKIDLGKDTDVQVYSPVKSQYEELNNYSPVMKIS
HHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCEEEECHHHHHHHHHCCCCCEEEEE
YGQNSFMFTGDAESLVEKEILNENKDLKADVLKLGHHGSHSSTSEEFLKAVDPSIAIVSC
ECCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEE
AKDNKYGHPHKETMSNLKKAGITVYETFRDGDITISSNGKKLDVKLHSENK
CCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCEEECCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]