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The map label for this gene is pfk [H]

Identifier: 18310449

GI number: 18310449

Start: 1723470

End: 1724672

Strand: Direct

Name: pfk [H]

Synonym: CPE1467

Alternate gene names: 18310449

Gene position: 1723470-1724672 (Clockwise)

Preceding gene: 18310439

Following gene: 18310452

Centisome position: 56.85

GC content: 29.93

Gene sequence:

>1203_bases
ATGAATTGTATTATAGCTCAGTCTGGCGGACCTACTTCAGTAATAAATGCTAGTGTTTATGGTCTAGTTAAAGCAAATAA
AAAACTTAATTTATATGAGAATGTTTATGGTGGATTAAACGGAATAGAAGGTATTTTAAATAAGAAAATAATAAACCTTT
CAGACCTTGATGAACTTCAATTAGAAAGATTTAAAAATTCACCAGCCTCTGGTTTAGGTTCTTGTAGATATAAATTAAAA
CCTCTTAGCGAAAGTGAAGATGAGTACTTAAAACTTTTAGATATTTTAAACTCACTTAATATAAAAGCTTTCTTTTATGT
AGGTGGAAATGACTCAATGGATACTACTGCTAAATTAAATGCTTTTGCAAAAGCTAAAAATATAGATATAAAATTCTTTG
GAATCCCTAAAACAATAGATAATGATTTAATGCACACAGATCACACTCCAGGATTTGGTAGCGCTGCAAAATTCATATCT
ACAGCAGTTTTAGAAACTTATTTAGACTCAAAAGTTTATACTAATAATGGAATTTTCATAATTGAAACTATGGGAAGAGA
TACAGGTTGGTTAGCAGCTTCTGGTGCCTTAGCAACTATAAACGGGAATAAATGTGCTGATTTAGTATATTTACCTGAAA
GAGCATTTTCAGTACAAAGCTTTTTAACTGATGTAGCTAGAATATTTAAAGAAAAAAAACATGTATATATAGTAGCTTCT
GAAGGATTAAAAGATGAAAATGGAAAGTTTCTTACTGAAACTGCACCAAATGACGATTCATTTGGACATGCTCAATTAGG
TGGTGTAGGTGCTTACTTAAAGAACCTAATAATCCAAGCTAGTATAACACATAGAGTTAAATCTCTAGAACTTAATACTC
TACAAAGATGTTCTATGCATGCTTCATCTAAAATAGATGTAAATGAAGCTGTTATGGTAGGACAAGCTGCCTTAGAAGCT
TCTATTAATTCAGAAGGTGGTTATATGGTTGCTATAAGAAGAGATTCTTCATTCCCATATAAAAGCTCTACTTTTTTAAT
TGAAGCTGATAAAATAGCTAATAATATAAAATATTTTCCAAGTGAATGGATAAATGAAGAAGGTAATAATATAACCGAAG
AAGGTTTATTATATCTTAAGCCATTAATTAGCGATTTTGAGGATAGAGATTCTCTTCCTCTTTATCATAGTTTTGTTTTA
TAA

Upstream 100 bases:

>100_bases
TAGTGTATATTATGAAATAGAGTACGTTATTAAGTTTAATAGTATAGCACATTTAACTTTTTTCATATAAATGTGCATAA
TAAAAGGAGAGATTTTTCAA

Downstream 100 bases:

>100_bases
ATATTATTTTACTCAATTACCTACATTTAATAAAAGACATGCATTTTCTGCATGTCTTTTATTTTTCTTTAAGAACATCC
TTTAAAGTATCATATGCAAA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNCIIAQSGGPTSVINASVYGLVKANKKLNLYENVYGGLNGIEGILNKKIINLSDLDELQLERFKNSPASGLGSCRYKLK
PLSESEDEYLKLLDILNSLNIKAFFYVGGNDSMDTTAKLNAFAKAKNIDIKFFGIPKTIDNDLMHTDHTPGFGSAAKFIS
TAVLETYLDSKVYTNNGIFIIETMGRDTGWLAASGALATINGNKCADLVYLPERAFSVQSFLTDVARIFKEKKHVYIVAS
EGLKDENGKFLTETAPNDDSFGHAQLGGVGAYLKNLIIQASITHRVKSLELNTLQRCSMHASSKIDVNEAVMVGQAALEA
SINSEGGYMVAIRRDSSFPYKSSTFLIEADKIANNIKYFPSEWINEEGNNITEEGLLYLKPLISDFEDRDSLPLYHSFVL

Sequences:

>Translated_400_residues
MNCIIAQSGGPTSVINASVYGLVKANKKLNLYENVYGGLNGIEGILNKKIINLSDLDELQLERFKNSPASGLGSCRYKLK
PLSESEDEYLKLLDILNSLNIKAFFYVGGNDSMDTTAKLNAFAKAKNIDIKFFGIPKTIDNDLMHTDHTPGFGSAAKFIS
TAVLETYLDSKVYTNNGIFIIETMGRDTGWLAASGALATINGNKCADLVYLPERAFSVQSFLTDVARIFKEKKHVYIVAS
EGLKDENGKFLTETAPNDDSFGHAQLGGVGAYLKNLIIQASITHRVKSLELNTLQRCSMHASSKIDVNEAVMVGQAALEA
SINSEGGYMVAIRRDSSFPYKSSTFLIEADKIANNIKYFPSEWINEEGNNITEEGLLYLKPLISDFEDRDSLPLYHSFVL
>Mature_400_residues
MNCIIAQSGGPTSVINASVYGLVKANKKLNLYENVYGGLNGIEGILNKKIINLSDLDELQLERFKNSPASGLGSCRYKLK
PLSESEDEYLKLLDILNSLNIKAFFYVGGNDSMDTTAKLNAFAKAKNIDIKFFGIPKTIDNDLMHTDHTPGFGSAAKFIS
TAVLETYLDSKVYTNNGIFIIETMGRDTGWLAASGALATINGNKCADLVYLPERAFSVQSFLTDVARIFKEKKHVYIVAS
EGLKDENGKFLTETAPNDDSFGHAQLGGVGAYLKNLIIQASITHRVKSLELNTLQRCSMHASSKIDVNEAVMVGQAALEA
SINSEGGYMVAIRRDSSFPYKSSTFLIEADKIANNIKYFPSEWINEEGNNITEEGLLYLKPLISDFEDRDSLPLYHSFVL

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=272, Percent_Identity=27.5735294117647, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 43997; Mature: 43997

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCIIAQSGGPTSVINASVYGLVKANKKLNLYENVYGGLNGIEGILNKKIINLSDLDELQ
CCEEEECCCCCCCEEEEEEEEEEECCCCCCHHHHHHCCCHHHHHHHHCCEECCCCCHHHH
LERFKNSPASGLGSCRYKLKPLSESEDEYLKLLDILNSLNIKAFFYVGGNDSMDTTAKLN
HHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
AFAKAKNIDIKFFGIPKTIDNDLMHTDHTPGFGSAAKFISTAVLETYLDSKVYTNNGIFI
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCEEE
IETMGRDTGWLAASGALATINGNKCADLVYLPERAFSVQSFLTDVARIFKEKKHVYIVAS
EEECCCCCCCEEECCEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEC
EGLKDENGKFLTETAPNDDSFGHAQLGGVGAYLKNLIIQASITHRVKSLELNTLQRCSMH
CCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
ASSKIDVNEAVMVGQAALEASINSEGGYMVAIRRDSSFPYKSSTFLIEADKIANNIKYFP
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHHCCCCCCC
SEWINEEGNNITEEGLLYLKPLISDFEDRDSLPLYHSFVL
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHEECC
>Mature Secondary Structure
MNCIIAQSGGPTSVINASVYGLVKANKKLNLYENVYGGLNGIEGILNKKIINLSDLDELQ
CCEEEECCCCCCCEEEEEEEEEEECCCCCCHHHHHHCCCHHHHHHHHCCEECCCCCHHHH
LERFKNSPASGLGSCRYKLKPLSESEDEYLKLLDILNSLNIKAFFYVGGNDSMDTTAKLN
HHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
AFAKAKNIDIKFFGIPKTIDNDLMHTDHTPGFGSAAKFISTAVLETYLDSKVYTNNGIFI
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCEEE
IETMGRDTGWLAASGALATINGNKCADLVYLPERAFSVQSFLTDVARIFKEKKHVYIVAS
EEECCCCCCCEEECCEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEC
EGLKDENGKFLTETAPNDDSFGHAQLGGVGAYLKNLIIQASITHRVKSLELNTLQRCSMH
CCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
ASSKIDVNEAVMVGQAALEASINSEGGYMVAIRRDSSFPYKSSTFLIEADKIANNIKYFP
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHHCCCCCCC
SEWINEEGNNITEEGLLYLKPLISDFEDRDSLPLYHSFVL
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550409 [H]