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The map label for this gene is thiI [H]

Identifier: 18310394

GI number: 18310394

Start: 1664692

End: 1665849

Strand: Reverse

Name: thiI [H]

Synonym: CPE1412

Alternate gene names: 18310394

Gene position: 1665849-1664692 (Counterclockwise)

Preceding gene: 18310395

Following gene: 18310393

Centisome position: 54.95

GC content: 30.74

Gene sequence:

>1158_bases
ATGAATAATTTAATTTTGGTAAAATATGCCTCAGAAATATTTTTAAAGGGGCTTAATAAAAATAAGTTTGAGAGAAAATT
AAAAGAAAATATAAGAAAAAAGTTAAAAGATATAGATCATGAATTTATAACAGATCAAAATAGATGGTTCATAAAATCAG
AAGACTTAGATGGAGTTATTGAAAGGGTAAAAAAGGTTTTTGGAGTTAAAGAACTTTGCTTAGTTACTCAGGTTACAGGG
GACTTTGATTCAATAAAAGAAGAGGGATTAAAGAAAATTAAAGAAAGCAAAGCTAAGAGTTTCAAAGTAGAAACAAATAG
AGCTAATAAAAAATTTCCGATGAATTCTATGGAGGTTTCAAGAGCTGTTGGAGGATATATCCTTTCAGAACTTGGGGATG
AAATAGAAGTTGATATACATAATCCAGAGTGTAAGCTTTATGTAGAAATAAGAGGAAATGCATATGTATTTACTGATAAA
GATAAAATAAAGGCTGTAGGAGGCTTACCATATGGAATGAACGGAAGTACTATGGTTATGTTATCAGGAGGAATTGATTC
ACCAGTAGCAGCTTATTTAATGGCTAGAAGAGGAGTTGAAACTCATTGTGTATATTATCATTCTCATCCATACACTTCAG
AAAGAGCCAAGGATAAGGTTAAGGAATTAGCAAAAATAGTAGGAAGATATACAGAAAAAATAACTCTTTATGTGGTTCCT
TTTACAGAAATACAAATGGATATAATAGAGAAGTGTAGAGAAGATGAATTAACAATAATAATGAGAAGATTCATGATGAG
AGTGGCTTGTGAACTTTCTGAAAAAAAGAAAATACAGTCAATAACTACTGGAGAAAGTATAGGGCAAGTGGCATCTCAGA
CTATGGAAGGACTTATAGTAAGTAATGATGCTTCTGATAGACCTGTATTTAGACCTCTAATAGCTATGGATAAAGAGGAT
ATAATGGATATTGCAAGAGATATAGATACTTATGAGACATCAATACTTCCATATGAAGATTGCTGCACAATATTTGTACC
AAAACATCCAAAGACTAAGCCTAGAGTTAAGGATATGATAATAGCAGAAAGAAAGCTTGATATAGAAGCTTTAGTAAATA
AGGCTATTGATGAAATGGAAACTTTCATATTTGAATAA

Upstream 100 bases:

>100_bases
CTATAAGATTTAGCTTTTCTAGATATAACACTTTAGAAGAAGTTGATTACACCATAGCTTCACTAGAAAAAGTTTTAAAA
TTTTTAAGGAGAATAAAAAT

Downstream 100 bases:

>100_bases
TTAGTTTTTTATAAGCTATTTATTGAGTTTTAAAGAAATTAAATATAAAATAAAAGGGCGTGTTTCAAAATAAGTTTTAT
TACTAAATTTTGATACAAAC

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MNNLILVKYASEIFLKGLNKNKFERKLKENIRKKLKDIDHEFITDQNRWFIKSEDLDGVIERVKKVFGVKELCLVTQVTG
DFDSIKEEGLKKIKESKAKSFKVETNRANKKFPMNSMEVSRAVGGYILSELGDEIEVDIHNPECKLYVEIRGNAYVFTDK
DKIKAVGGLPYGMNGSTMVMLSGGIDSPVAAYLMARRGVETHCVYYHSHPYTSERAKDKVKELAKIVGRYTEKITLYVVP
FTEIQMDIIEKCREDELTIIMRRFMMRVACELSEKKKIQSITTGESIGQVASQTMEGLIVSNDASDRPVFRPLIAMDKED
IMDIARDIDTYETSILPYEDCCTIFVPKHPKTKPRVKDMIIAERKLDIEALVNKAIDEMETFIFE

Sequences:

>Translated_385_residues
MNNLILVKYASEIFLKGLNKNKFERKLKENIRKKLKDIDHEFITDQNRWFIKSEDLDGVIERVKKVFGVKELCLVTQVTG
DFDSIKEEGLKKIKESKAKSFKVETNRANKKFPMNSMEVSRAVGGYILSELGDEIEVDIHNPECKLYVEIRGNAYVFTDK
DKIKAVGGLPYGMNGSTMVMLSGGIDSPVAAYLMARRGVETHCVYYHSHPYTSERAKDKVKELAKIVGRYTEKITLYVVP
FTEIQMDIIEKCREDELTIIMRRFMMRVACELSEKKKIQSITTGESIGQVASQTMEGLIVSNDASDRPVFRPLIAMDKED
IMDIARDIDTYETSILPYEDCCTIFVPKHPKTKPRVKDMIIAERKLDIEALVNKAIDEMETFIFE
>Mature_385_residues
MNNLILVKYASEIFLKGLNKNKFERKLKENIRKKLKDIDHEFITDQNRWFIKSEDLDGVIERVKKVFGVKELCLVTQVTG
DFDSIKEEGLKKIKESKAKSFKVETNRANKKFPMNSMEVSRAVGGYILSELGDEIEVDIHNPECKLYVEIRGNAYVFTDK
DKIKAVGGLPYGMNGSTMVMLSGGIDSPVAAYLMARRGVETHCVYYHSHPYTSERAKDKVKELAKIVGRYTEKITLYVVP
FTEIQMDIIEKCREDELTIIMRRFMMRVACELSEKKKIQSITTGESIGQVASQTMEGLIVSNDASDRPVFRPLIAMDKED
IMDIARDIDTYETSILPYEDCCTIFVPKHPKTKPRVKDMIIAERKLDIEALVNKAIDEMETFIFE

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=383, Percent_Identity=27.6762402088773, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 44132; Mature: 44132

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLILVKYASEIFLKGLNKNKFERKLKENIRKKLKDIDHEFITDQNRWFIKSEDLDGVI
CCCEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEECCCHHHHH
ERVKKVFGVKELCLVTQVTGDFDSIKEEGLKKIKESKAKSFKVETNRANKKFPMNSMEVS
HHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHH
RAVGGYILSELGDEIEVDIHNPECKLYVEIRGNAYVFTDKDKIKAVGGLPYGMNGSTMVM
HHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCEEEEECHHHHHEECCCCCCCCCCEEEE
LSGGIDSPVAAYLMARRGVETHCVYYHSHPYTSERAKDKVKELAKIVGRYTEKITLYVVP
EECCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEE
FTEIQMDIIEKCREDELTIIMRRFMMRVACELSEKKKIQSITTGESIGQVASQTMEGLIV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEE
SNDASDRPVFRPLIAMDKEDIMDIARDIDTYETSILPYEDCCTIFVPKHPKTKPRVKDMI
ECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHCEEEEECCCCCCCCHHHHHH
IAERKLDIEALVNKAIDEMETFIFE
HHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNLILVKYASEIFLKGLNKNKFERKLKENIRKKLKDIDHEFITDQNRWFIKSEDLDGVI
CCCEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEECCCHHHHH
ERVKKVFGVKELCLVTQVTGDFDSIKEEGLKKIKESKAKSFKVETNRANKKFPMNSMEVS
HHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHH
RAVGGYILSELGDEIEVDIHNPECKLYVEIRGNAYVFTDKDKIKAVGGLPYGMNGSTMVM
HHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCEEEEECHHHHHEECCCCCCCCCCEEEE
LSGGIDSPVAAYLMARRGVETHCVYYHSHPYTSERAKDKVKELAKIVGRYTEKITLYVVP
EECCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEE
FTEIQMDIIEKCREDELTIIMRRFMMRVACELSEKKKIQSITTGESIGQVASQTMEGLIV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEE
SNDASDRPVFRPLIAMDKEDIMDIARDIDTYETSILPYEDCCTIFVPKHPKTKPRVKDMI
ECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHCEEEEECCCCCCCCHHHHHH
IAERKLDIEALVNKAIDEMETFIFE
HHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA