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The map label for this gene is nth [H]

Identifier: 18310300

GI number: 18310300

Start: 1560366

End: 1560995

Strand: Reverse

Name: nth [H]

Synonym: CPE1318

Alternate gene names: 18310300

Gene position: 1560995-1560366 (Counterclockwise)

Preceding gene: 18310301

Following gene: 18310298

Centisome position: 51.49

GC content: 30.95

Gene sequence:

>630_bases
ATGAAAAAGAGAACTAAAGAGGTATTAGAAATATTAAAAGAAGAGTATCCAGATGCTAAGTGTGAGCTAAATTATGAAAC
TCCCTTTCAACTTTTAGTTGCCACTATTTTATCAGCACAAACTACTGATAAAAAGGTTAATGAGGTTACTAAAGGGCTTT
TTAAGGACTATCCAGATGTAGATTCCTTTTTAACTATAAGCCAAGAGGAGCTAGAGGATAGAATAAAGCAAATTGGACTT
TATAGAAATAAAGCTAAAAATTTAATTATGATGGTTCATCAATTAAAAGAGAATTTTGGTGGAGAAGTTCCTAAAACTAT
GGAAGGAATAACTTCCTTAGCGGGAGCAGGAAGAAAAACTGCCAATGTGGTTTTATCAAATGCTTTTGGAGTTCCATCTA
TTGCAGTAGATACTCACGTCTTTAGAGTTTCAAATAGAATTGGCTTAGCCCATTCAGATAATGTGCTTGAAACTGAAAAA
CAACTTCAAAAAGAATTACCTAAAAAAGAATGGTCCTTAACCCATCATCTTTTAATTTTTCATGGCAGAAGATGTTGCAT
AGCTAGAAAGCCTAAATGTGATATATGTAAGATAAATAAATATTGTGATTATTTTAAAAATAATAAATAA

Upstream 100 bases:

>100_bases
ATGAGTATGAAGAAAGAGCTTTAAATTATCTTATGAAAAATATAGCCAATCTTTTACCAGAAGATTATAGAGGGGTATAT
AAGGATTAGGGGGACATATT

Downstream 100 bases:

>100_bases
AAAGCTACACAAAATAGTGTAGCTTTTTTTATTTGCTAGCTTCTTTTTTGTTATATAAGAATATAGAAAAAGATGCTAAT
AAAATTAAGGCATATCCTAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKKRTKEVLEILKEEYPDAKCELNYETPFQLLVATILSAQTTDKKVNEVTKGLFKDYPDVDSFLTISQEELEDRIKQIGL
YRNKAKNLIMMVHQLKENFGGEVPKTMEGITSLAGAGRKTANVVLSNAFGVPSIAVDTHVFRVSNRIGLAHSDNVLETEK
QLQKELPKKEWSLTHHLLIFHGRRCCIARKPKCDICKINKYCDYFKNNK

Sequences:

>Translated_209_residues
MKKRTKEVLEILKEEYPDAKCELNYETPFQLLVATILSAQTTDKKVNEVTKGLFKDYPDVDSFLTISQEELEDRIKQIGL
YRNKAKNLIMMVHQLKENFGGEVPKTMEGITSLAGAGRKTANVVLSNAFGVPSIAVDTHVFRVSNRIGLAHSDNVLETEK
QLQKELPKKEWSLTHHLLIFHGRRCCIARKPKCDICKINKYCDYFKNNK
>Mature_209_residues
MKKRTKEVLEILKEEYPDAKCELNYETPFQLLVATILSAQTTDKKVNEVTKGLFKDYPDVDSFLTISQEELEDRIKQIGL
YRNKAKNLIMMVHQLKENFGGEVPKTMEGITSLAGAGRKTANVVLSNAFGVPSIAVDTHVFRVSNRIGLAHSDNVLETEK
QLQKELPKKEWSLTHHLLIFHGRRCCIARKPKCDICKINKYCDYFKNNK

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=30.5084745762712, Blast_Score=97, Evalue=8e-21,
Organism=Escherichia coli, GI1787920, Length=203, Percent_Identity=45.8128078817734, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1789331, Length=196, Percent_Identity=24.4897959183673, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=176, Percent_Identity=38.0681818181818, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324530, Length=188, Percent_Identity=29.2553191489362, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=27.0114942528736, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45550361, Length=183, Percent_Identity=32.7868852459016, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23915; Mature: 23915

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRTKEVLEILKEEYPDAKCELNYETPFQLLVATILSAQTTDKKVNEVTKGLFKDYPDV
CCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
DSFLTISQEELEDRIKQIGLYRNKAKNLIMMVHQLKENFGGEVPKTMEGITSLAGAGRKT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHH
ANVVLSNAFGVPSIAVDTHVFRVSNRIGLAHSDNVLETEKQLQKELPKKEWSLTHHLLIF
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
HGRRCCIARKPKCDICKINKYCDYFKNNK
CCCEEEECCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKRTKEVLEILKEEYPDAKCELNYETPFQLLVATILSAQTTDKKVNEVTKGLFKDYPDV
CCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
DSFLTISQEELEDRIKQIGLYRNKAKNLIMMVHQLKENFGGEVPKTMEGITSLAGAGRKT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHH
ANVVLSNAFGVPSIAVDTHVFRVSNRIGLAHSDNVLETEKQLQKELPKKEWSLTHHLLIF
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
HGRRCCIARKPKCDICKINKYCDYFKNNK
CCCEEEECCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]