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The map label for this gene is dusC [H]

Identifier: 18310257

GI number: 18310257

Start: 1495134

End: 1496099

Strand: Reverse

Name: dusC [H]

Synonym: CPE1275

Alternate gene names: 18310257

Gene position: 1496099-1495134 (Counterclockwise)

Preceding gene: 18310258

Following gene: 18310256

Centisome position: 49.35

GC content: 28.36

Gene sequence:

>966_bases
ATGAAGTATTATTTAGCACCAATGGAAGGGATTACTGGATTTATATATAGAAATTCTTATGAAAAGTTTTTTGGCGGAAT
TGATAAATACTTTGCCCCTTTTGTTGTTCCAAATAGTAGTAAAAGTCTTAAAACTAAGGAATTAAGAGATGTTTTGCCAG
AGAATAATAAAGGAATAAACTTAGTTCCACAAATACTTACCAATGATTCAGAAGGGTTTATTTTAACAGCTAAAAAGCTT
AAGGATTTAGGATATAATGAAATAAATTTAAACTTAGGATGTCCCTCTGGAACAGTTGTTGGTAAGAAAAGAGGGTCAGG
TTTTTTAGCTCACAAGGAAGAACTTGATAAGTTTTTAGAAGAAATATTTAAAATAGATGACATAAAAATTTCAATAAAAA
CTAGATTAGGAATGGATAAGCCTGAGGAATTTTATGAACTTATAAAAATTTATAATAAGTATCCTATGGAGGAGCTTATT
ATTCATCCTAGAACAAGACAAGATTTTTATGGAAATAAACCTAATTTAGAGGTTTTTAAAGATGCAATTTCTTTAAGTAA
GAATCCAGTTTGTTATAATGGAGATATTTTTACCTTAGAGGATCATAATAAATTGGTTGAAGAATTCAAAGAAGTGGATA
AAATAATGTTAGGAAGAGGGGTCTTAGCTAACCCAGCCTTAATGAATGAAATTTTAAACAATGAGTTTATGGACAAAAAG
GTATTAAAAGAGTTTCATGATGAAATATTTAGTAAGTACAGAGAAGTATTTAATGAAGATAGAAATGCAATGTTTAGAAT
GAAGGAGCTATGGGGATATATGATATATATGTTCTCAAATAATAAAAAATATGCTAAGAAAATAAAGAAAGCTCAGAAAG
TAGTTGATTATAACCAAGCTGTTACAAGTCTATTTTTAGAACAGGATATACTTAAGGGAGCTGGATTATTTAGTAGTGAA
GAGTAA

Upstream 100 bases:

>100_bases
ATTAAAGAAATTTACATAAAAATACTTTAATTACTAAGTGGATAAGAAGAAAAAAGCCTGTTATAATATTTTCTAGAATC
TTTAAAAAAAGGAGAAACAA

Downstream 100 bases:

>100_bases
ATATAAGATATGTTTCTTTATATTTAATATATTTATTTTAAAATCTATCAGACTTTAGTTTTATTGTCTGGTAGATTTTT
TATTATAGGAATAAGAATAG

Product: dihydrouridine synthase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKYYLAPMEGITGFIYRNSYEKFFGGIDKYFAPFVVPNSSKSLKTKELRDVLPENNKGINLVPQILTNDSEGFILTAKKL
KDLGYNEINLNLGCPSGTVVGKKRGSGFLAHKEELDKFLEEIFKIDDIKISIKTRLGMDKPEEFYELIKIYNKYPMEELI
IHPRTRQDFYGNKPNLEVFKDAISLSKNPVCYNGDIFTLEDHNKLVEEFKEVDKIMLGRGVLANPALMNEILNNEFMDKK
VLKEFHDEIFSKYREVFNEDRNAMFRMKELWGYMIYMFSNNKKYAKKIKKAQKVVDYNQAVTSLFLEQDILKGAGLFSSE
E

Sequences:

>Translated_321_residues
MKYYLAPMEGITGFIYRNSYEKFFGGIDKYFAPFVVPNSSKSLKTKELRDVLPENNKGINLVPQILTNDSEGFILTAKKL
KDLGYNEINLNLGCPSGTVVGKKRGSGFLAHKEELDKFLEEIFKIDDIKISIKTRLGMDKPEEFYELIKIYNKYPMEELI
IHPRTRQDFYGNKPNLEVFKDAISLSKNPVCYNGDIFTLEDHNKLVEEFKEVDKIMLGRGVLANPALMNEILNNEFMDKK
VLKEFHDEIFSKYREVFNEDRNAMFRMKELWGYMIYMFSNNKKYAKKIKKAQKVVDYNQAVTSLFLEQDILKGAGLFSSE
E
>Mature_321_residues
MKYYLAPMEGITGFIYRNSYEKFFGGIDKYFAPFVVPNSSKSLKTKELRDVLPENNKGINLVPQILTNDSEGFILTAKKL
KDLGYNEINLNLGCPSGTVVGKKRGSGFLAHKEELDKFLEEIFKIDDIKISIKTRLGMDKPEEFYELIKIYNKYPMEELI
IHPRTRQDFYGNKPNLEVFKDAISLSKNPVCYNGDIFTLEDHNKLVEEFKEVDKIMLGRGVLANPALMNEILNNEFMDKK
VLKEFHDEIFSKYREVFNEDRNAMFRMKELWGYMIYMFSNNKKYAKKIKKAQKVVDYNQAVTSLFLEQDILKGAGLFSSE
E

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788462, Length=245, Percent_Identity=26.530612244898, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789660, Length=259, Percent_Identity=25.4826254826255, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37322; Mature: 37322

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYYLAPMEGITGFIYRNSYEKFFGGIDKYFAPFVVPNSSKSLKTKELRDVLPENNKGIN
CCEEECCHHHHEEHEECCCHHHHHCCHHHHHCCEEECCCCCCCHHHHHHHHCCCCCCCCE
LVPQILTNDSEGFILTAKKLKDLGYNEINLNLGCPSGTVVGKKRGSGFLAHKEELDKFLE
EHHHHHCCCCCCEEEEHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHH
EIFKIDDIKISIKTRLGMDKPEEFYELIKIYNKYPMEELIIHPRTRQDFYGNKPNLEVFK
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHCCCCCCCHHHHH
DAISLSKNPVCYNGDIFTLEDHNKLVEEFKEVDKIMLGRGVLANPALMNEILNNEFMDKK
HHHHCCCCCEEECCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHH
VLKEFHDEIFSKYREVFNEDRNAMFRMKELWGYMIYMFSNNKKYAKKIKKAQKVVDYNQA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHHH
VTSLFLEQDILKGAGLFSSEE
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKYYLAPMEGITGFIYRNSYEKFFGGIDKYFAPFVVPNSSKSLKTKELRDVLPENNKGIN
CCEEECCHHHHEEHEECCCHHHHHCCHHHHHCCEEECCCCCCCHHHHHHHHCCCCCCCCE
LVPQILTNDSEGFILTAKKLKDLGYNEINLNLGCPSGTVVGKKRGSGFLAHKEELDKFLE
EHHHHHCCCCCCEEEEHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHH
EIFKIDDIKISIKTRLGMDKPEEFYELIKIYNKYPMEELIIHPRTRQDFYGNKPNLEVFK
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHCCCCCCCHHHHH
DAISLSKNPVCYNGDIFTLEDHNKLVEEFKEVDKIMLGRGVLANPALMNEILNNEFMDKK
HHHHCCCCCEEECCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHH
VLKEFHDEIFSKYREVFNEDRNAMFRMKELWGYMIYMFSNNKKYAKKIKKAQKVVDYNQA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHHH
VTSLFLEQDILKGAGLFSSEE
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]