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The map label for this gene is potD-B [H]

Identifier: 18310251

GI number: 18310251

Start: 1489634

End: 1490680

Strand: Reverse

Name: potD-B [H]

Synonym: CPE1269

Alternate gene names: 18310251

Gene position: 1490680-1489634 (Counterclockwise)

Preceding gene: 18310252

Following gene: 18310250

Centisome position: 49.17

GC content: 30.47

Gene sequence:

>1047_bases
ATGAAAAAGAAAATTTTAGCAACCTTATTAACAGGATTAGTACTAGGGACATCTTTAGTTGGATGTGGAAAAACAGAAGG
AGCAGAGGCAGGAAAAAAATTAGTAGTTTCAACTTGGGGATTAAATGAAGATGTATTAAAGGAGACAGTATTTGAACCAT
TTGCTAAAGAACATGGTGTGGAAATAGTTTTAGATATAGGTAATAACTCAGAAAGATTAACTAAGATGAAAAATAATCCT
AACTCACAAATAGATATAACTTATTTAGCAGAATCTTTTGCAGAGCAAGGAGTAGAAGCAGGTATATTCGATAAATTAGA
TTATTCAAAGATTCCTAATGCAAGTGAGATGAATGAAAAGGCTAAATCTACAGTAGAGGCAGGATATGGACCAGCATATA
CATTAAATAGTATAGGAATAGTTGTAGATCCTTCAGCAGGAATTGAAATAAATTCTTGGGAAGATTTATGGAAACCAGAA
CTTAAAAATAAAATAGCTATACCAGATATTACAACAACTAATGGTCCAGCAATGGTAGAAATAGCAGCAGAAAAGGCAGG
AGTAGATGTTAAAACTGATAATGGAGAAGCTGCATTTAAAGAATTAGAAGCTTTAAAACCAAATGTTGTTAAAACTTATA
GTAAATCTTCAGATTTAGCTAATATGTTCTCAAATGGTGAAATAGTAGCTGCTGTAGCATCAGACTTTGCTTTTGGAACA
ATTTCAAAAGCTAAACCAGAGGTTATAAATGTAATACCTGAGTCAGGAACTTACTTAAACTTTAATACAATAAATATAAA
TAAAAATTCTAAAAATAAAGATTTAGCTTATGAATTTATTAACTATGCATTAAGCAAAGAAGTTCAGGAGAAAACTGCTA
AAGCTTTAAATGAATCACCAGTTAATAAAGAAGTTAAATTAAATGGAGAAGAAACTAAAAACTTAACATATGGACCAGTG
GTTGATAACGCTAAGGTAATTGACTTTAAGTTTGTTAATTCAGTAATGGATCAATGGGTTAATAACTGGAACAGAATAAT
GAACTAG

Upstream 100 bases:

>100_bases
ATATGAAAAGGGACAAAATATAAAACTTCAATTACCAGTGGATAAAATAGTAATTTTATAAAACTTAAATATAAAAAATA
TGTTTATAAAGGAGAATCTT

Downstream 100 bases:

>100_bases
TTTGCAGGTAGTGAAATGCAAGTAGAATTAATTATTAAAAATTTAAAGGTATATAATAGTTATTTTAAAAAATTCATAAA
AAGTGATGTCTTAATAAATG

Product: spermidine/putrescine-binding protein 1

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKKKILATLLTGLVLGTSLVGCGKTEGAEAGKKLVVSTWGLNEDVLKETVFEPFAKEHGVEIVLDIGNNSERLTKMKNNP
NSQIDITYLAESFAEQGVEAGIFDKLDYSKIPNASEMNEKAKSTVEAGYGPAYTLNSIGIVVDPSAGIEINSWEDLWKPE
LKNKIAIPDITTTNGPAMVEIAAEKAGVDVKTDNGEAAFKELEALKPNVVKTYSKSSDLANMFSNGEIVAAVASDFAFGT
ISKAKPEVINVIPESGTYLNFNTININKNSKNKDLAYEFINYALSKEVQEKTAKALNESPVNKEVKLNGEETKNLTYGPV
VDNAKVIDFKFVNSVMDQWVNNWNRIMN

Sequences:

>Translated_348_residues
MKKKILATLLTGLVLGTSLVGCGKTEGAEAGKKLVVSTWGLNEDVLKETVFEPFAKEHGVEIVLDIGNNSERLTKMKNNP
NSQIDITYLAESFAEQGVEAGIFDKLDYSKIPNASEMNEKAKSTVEAGYGPAYTLNSIGIVVDPSAGIEINSWEDLWKPE
LKNKIAIPDITTTNGPAMVEIAAEKAGVDVKTDNGEAAFKELEALKPNVVKTYSKSSDLANMFSNGEIVAAVASDFAFGT
ISKAKPEVINVIPESGTYLNFNTININKNSKNKDLAYEFINYALSKEVQEKTAKALNESPVNKEVKLNGEETKNLTYGPV
VDNAKVIDFKFVNSVMDQWVNNWNRIMN
>Mature_348_residues
MKKKILATLLTGLVLGTSLVGCGKTEGAEAGKKLVVSTWGLNEDVLKETVFEPFAKEHGVEIVLDIGNNSERLTKMKNNP
NSQIDITYLAESFAEQGVEAGIFDKLDYSKIPNASEMNEKAKSTVEAGYGPAYTLNSIGIVVDPSAGIEINSWEDLWKPE
LKNKIAIPDITTTNGPAMVEIAAEKAGVDVKTDNGEAAFKELEALKPNVVKTYSKSSDLANMFSNGEIVAAVASDFAFGT
ISKAKPEVINVIPESGTYLNFNTININKNSKNKDLAYEFINYALSKEVQEKTAKALNESPVNKEVKLNGEETKNLTYGPV
VDNAKVIDFKFVNSVMDQWVNNWNRIMN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=304, Percent_Identity=27.3026315789474, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787367, Length=264, Percent_Identity=26.1363636363636, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37970; Mature: 37970

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKILATLLTGLVLGTSLVGCGKTEGAEAGKKLVVSTWGLNEDVLKETVFEPFAKEHGV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCC
EIVLDIGNNSERLTKMKNNPNSQIDITYLAESFAEQGVEAGIFDKLDYSKIPNASEMNEK
EEEEECCCCHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHHH
AKSTVEAGYGPAYTLNSIGIVVDPSAGIEINSWEDLWKPELKNKIAIPDITTTNGPAMVE
HHHHHHCCCCCCEEECCCCEEECCCCCEEECCCHHHCCHHHCCEEECCCEECCCCCEEEE
IAAEKAGVDVKTDNGEAAFKELEALKPNVVKTYSKSSDLANMFSNGEIVAAVASDFAFGT
EEHHHCCCEEEECCCHHHHHHHHHHCCCHHHHHCCCHHHHHHHCCCCEEEEECCCCHHCC
ISKAKPEVINVIPESGTYLNFNTININKNSKNKDLAYEFINYALSKEVQEKTAKALNESP
CCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VNKEVKLNGEETKNLTYGPVVDNAKVIDFKFVNSVMDQWVNNWNRIMN
CCCEEEECCCCCCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKILATLLTGLVLGTSLVGCGKTEGAEAGKKLVVSTWGLNEDVLKETVFEPFAKEHGV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCC
EIVLDIGNNSERLTKMKNNPNSQIDITYLAESFAEQGVEAGIFDKLDYSKIPNASEMNEK
EEEEECCCCHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHHH
AKSTVEAGYGPAYTLNSIGIVVDPSAGIEINSWEDLWKPELKNKIAIPDITTTNGPAMVE
HHHHHHCCCCCCEEECCCCEEECCCCCEEECCCHHHCCHHHCCEEECCCEECCCCCEEEE
IAAEKAGVDVKTDNGEAAFKELEALKPNVVKTYSKSSDLANMFSNGEIVAAVASDFAFGT
EEHHHCCCEEEECCCHHHHHHHHHHCCCHHHHHCCCHHHHHHHCCCCEEEEECCCCHHCC
ISKAKPEVINVIPESGTYLNFNTININKNSKNKDLAYEFINYALSKEVQEKTAKALNESP
CCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VNKEVKLNGEETKNLTYGPVVDNAKVIDFKFVNSVMDQWVNNWNRIMN
CCCEEEECCCCCCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]