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The map label for this gene is greA [H]

Identifier: 18310245

GI number: 18310245

Start: 1474429

End: 1474893

Strand: Direct

Name: greA [H]

Synonym: CPE1263

Alternate gene names: 18310245

Gene position: 1474429-1474893 (Clockwise)

Preceding gene: 18310244

Following gene: 18310247

Centisome position: 48.64

GC content: 32.04

Gene sequence:

>465_bases
ATGAACAATTATTTGACAGAGGAAAATGTTAGAAAGTTAAGAGAAGAATTAGACTATAGAATGTCTGTTGTTAGAGCAAA
GATTGCGAAAGAGAAACTTGAGGCAGCAGCCCATGGTGATAGATCAGAAAACGCTGAGTATAAGGAAGCTTGTAGAAACT
ATAGAGAAAATGATAATAGAATACAATACCTTTTAACTATGATTTCAACAGCAATAATAGTTGATGAAGAAAGTAATGAT
AAATCTACACTAGGTCTTAACAGCAAAGCTAGAATTAAATTTGTTGAAGATGATGATGAGGATGTTGTTACTTTAGTTAC
AACTATGGATTTAGATTTAGAAAACATGTGTATAAGCATAGAATCAGATTTAGGAAAGGCCCTAATGGGTAAAAAGGTTG
GAGATGTAGTAGAAGTTGAAGCTCCTGGTGGAAACTATACTGTAGAAGTATTAGAAATATTATAA

Upstream 100 bases:

>100_bases
CCTATAAATACTACCCTATTTCCTAATTAACACCTTTAAAATTTATGTAAATGTTGTATAATGATAAAATAAAGAATTAA
GAGTTAAGGGAGATTTTATT

Downstream 100 bases:

>100_bases
TAGACTTAGCTTAACTTATAAAAATAGTTTACTACTATATTGTACAGTAGAAATTTTTCTTTAAATTTAAAACTTATAAA
TTATGCTATATTCTAATATT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MNNYLTEENVRKLREELDYRMSVVRAKIAKEKLEAAAHGDRSENAEYKEACRNYRENDNRIQYLLTMISTAIIVDEESND
KSTLGLNSKARIKFVEDDDEDVVTLVTTMDLDLENMCISIESDLGKALMGKKVGDVVEVEAPGGNYTVEVLEIL

Sequences:

>Translated_154_residues
MNNYLTEENVRKLREELDYRMSVVRAKIAKEKLEAAAHGDRSENAEYKEACRNYRENDNRIQYLLTMISTAIIVDEESND
KSTLGLNSKARIKFVEDDDEDVVTLVTTMDLDLENMCISIESDLGKALMGKKVGDVVEVEAPGGNYTVEVLEIL
>Mature_154_residues
MNNYLTEENVRKLREELDYRMSVVRAKIAKEKLEAAAHGDRSENAEYKEACRNYRENDNRIQYLLTMISTAIIVDEESND
KSTLGLNSKARIKFVEDDDEDVVTLVTTMDLDLENMCISIESDLGKALMGKKVGDVVEVEAPGGNYTVEVLEIL

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=144, Percent_Identity=32.6388888888889, Blast_Score=70, Evalue=7e-14,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=31.5789473684211, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17440; Mature: 17440

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNYLTEENVRKLREELDYRMSVVRAKIAKEKLEAAAHGDRSENAEYKEACRNYRENDNR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCH
IQYLLTMISTAIIVDEESNDKSTLGLNSKARIKFVEDDDEDVVTLVTTMDLDLENMCISI
HHHHHHHHHHHEEEECCCCCCCEECCCCCEEEEEECCCCCCEEEEEEECCCCHHHHEEEE
ESDLGKALMGKKVGDVVEVEAPGGNYTVEVLEIL
HHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEC
>Mature Secondary Structure
MNNYLTEENVRKLREELDYRMSVVRAKIAKEKLEAAAHGDRSENAEYKEACRNYRENDNR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCH
IQYLLTMISTAIIVDEESNDKSTLGLNSKARIKFVEDDDEDVVTLVTTMDLDLENMCISI
HHHHHHHHHHHEEEECCCCCCCEECCCCCEEEEEECCCCCCEEEEEEECCCCHHHHEEEE
ESDLGKALMGKKVGDVVEVEAPGGNYTVEVLEIL
HHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA