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The map label for this gene is yfmF [H]

Identifier: 18310197

GI number: 18310197

Start: 1420122

End: 1420595

Strand: Reverse

Name: yfmF [H]

Synonym: CPE1215

Alternate gene names: 18310197

Gene position: 1420595-1420122 (Counterclockwise)

Preceding gene: 18310198

Following gene: 18310196

Centisome position: 46.86

GC content: 26.79

Gene sequence:

>474_bases
TTGTTCTTATGGGAAGATAATCCATATAAAAGGACTTTTCAATTAGACAATAAAGAAGATTATGACATAGTAAGAGAGGC
TTTAGAAAAGGTTAATATGCTTGAATATAAGGATAGAGATTTTTTGAATCTTTCAGGAGGAGAAAAACAAAGGGTATTAT
TAGCAAGAGCCTTAGCACAAAAAACAGAAATTCTTTTATTAGATGAACCTACAAATCATTTAGATATTAAGTATCAAATA
GAACGAATGAATATAGTTAAAGAGTTAAATATAACGGTATTAGCTGCACTTCATGATATGAATTTAGTTTCAGCTTACTG
TAATAAAGTATATATGATTGATAAAGGAAAAGTAGTTTATGGAGGAGAAACAAAAGAAGTTTTAACTCCTAAAAATATAA
AAGATGTTTTTGGTGTTGAATGTGAAATAGGAGAAAATTCAAAAGGACATATAACAATAAACTTTTTATTTTAG

Upstream 100 bases:

>100_bases
GGTAAAGAAATAGACAGTATTAGTATAAAGTCAATGGCAAGAGAATTAGCAGTAGTAAGCCAATTTAATCAATATAATTT
TAGCTTTAAGGTTAAAGATA

Downstream 100 bases:

>100_bases
ATAATGACTCTGTTTTAAGATTATAACTTAAGGCAGAGTTTTTATTATATAAACATGGAATTATTTAAAGTTAGTGTTGA
TTTTAGTTAGATATAAATAT

Product: ABC transporter

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: Ferric-citrate import ATP-binding protein [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MFLWEDNPYKRTFQLDNKEDYDIVREALEKVNMLEYKDRDFLNLSGGEKQRVLLARALAQKTEILLLDEPTNHLDIKYQI
ERMNIVKELNITVLAALHDMNLVSAYCNKVYMIDKGKVVYGGETKEVLTPKNIKDVFGVECEIGENSKGHITINFLF

Sequences:

>Translated_157_residues
MFLWEDNPYKRTFQLDNKEDYDIVREALEKVNMLEYKDRDFLNLSGGEKQRVLLARALAQKTEILLLDEPTNHLDIKYQI
ERMNIVKELNITVLAALHDMNLVSAYCNKVYMIDKGKVVYGGETKEVLTPKNIKDVFGVECEIGENSKGHITINFLF
>Mature_157_residues
MFLWEDNPYKRTFQLDNKEDYDIVREALEKVNMLEYKDRDFLNLSGGEKQRVLLARALAQKTEILLLDEPTNHLDIKYQI
ERMNIVKELNITVLAALHDMNLVSAYCNKVYMIDKGKVVYGGETKEVLTPKNIKDVFGVECEIGENSKGHITINFLF

Specific function: Part of the ABC transporter complex YfmCDEF involved in citrate-dependent Fe(3+) import. Responsible for energy coupling to the transport system (Probable) [H]

COG id: COG1120

COG function: function code PH; ABC-type cobalamin/Fe3+-siderophores transport systems, ATPase components

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI1790739, Length=140, Percent_Identity=39.2857142857143, Blast_Score=105, Evalue=1e-24,
Organism=Escherichia coli, GI1786803, Length=140, Percent_Identity=37.1428571428571, Blast_Score=103, Evalue=4e-24,
Organism=Escherichia coli, GI1786345, Length=154, Percent_Identity=29.8701298701299, Blast_Score=94, Evalue=4e-21,
Organism=Escherichia coli, GI1790544, Length=126, Percent_Identity=31.7460317460317, Blast_Score=76, Evalue=9e-16,
Organism=Escherichia coli, GI87081791, Length=118, Percent_Identity=35.5932203389831, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1787089, Length=125, Percent_Identity=31.2, Blast_Score=71, Evalue=3e-14,
Organism=Escherichia coli, GI1789864, Length=138, Percent_Identity=30.4347826086957, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1787029, Length=113, Percent_Identity=33.6283185840708, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789672, Length=123, Percent_Identity=30.8943089430894, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1787712, Length=108, Percent_Identity=34.2592592592593, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1788225, Length=105, Percent_Identity=34.2857142857143, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1789873, Length=106, Percent_Identity=38.6792452830189, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1787759, Length=121, Percent_Identity=30.5785123966942, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1786398, Length=128, Percent_Identity=32.8125, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI1788002, Length=111, Percent_Identity=34.2342342342342, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI48994883, Length=149, Percent_Identity=30.8724832214765, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1787758, Length=99, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI87081709, Length=108, Percent_Identity=35.1851851851852, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1788506, Length=102, Percent_Identity=35.2941176470588, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1788165, Length=127, Percent_Identity=34.6456692913386, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1789891, Length=127, Percent_Identity=33.0708661417323, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789593, Length=126, Percent_Identity=30.1587301587302, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1786872, Length=95, Percent_Identity=34.7368421052632, Blast_Score=63, Evalue=8e-12,
Organism=Escherichia coli, GI1787164, Length=79, Percent_Identity=41.7721518987342, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1787041, Length=93, Percent_Identity=38.7096774193548, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1788761, Length=99, Percent_Identity=37.3737373737374, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI145693107, Length=89, Percent_Identity=32.5842696629214, Blast_Score=61, Evalue=3e-11,
Organism=Escherichia coli, GI1787548, Length=111, Percent_Identity=32.4324324324324, Blast_Score=60, Evalue=5e-11,
Organism=Escherichia coli, GI1787370, Length=115, Percent_Identity=32.1739130434783, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 18246; Mature: 18246

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLWEDNPYKRTFQLDNKEDYDIVREALEKVNMLEYKDRDFLNLSGGEKQRVLLARALAQ
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEECCCCCEEECCCCCHHHHHHHHHHHC
KTEILLLDEPTNHLDIKYQIERMNIVKELNITVLAALHDMNLVSAYCNKVYMIDKGKVVY
CCEEEEEECCCCCEEEEEEEEEECHHHHCCEEEEEEEHHHHHHHHHHCEEEEEECCEEEE
GGETKEVLTPKNIKDVFGVECEIGENSKGHITINFLF
CCCCCEEECCCCCHHHHCCEEEECCCCCCEEEEEEEC
>Mature Secondary Structure
MFLWEDNPYKRTFQLDNKEDYDIVREALEKVNMLEYKDRDFLNLSGGEKQRVLLARALAQ
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEECCCCCEEECCCCCHHHHHHHHHHHC
KTEILLLDEPTNHLDIKYQIERMNIVKELNITVLAALHDMNLVSAYCNKVYMIDKGKVVY
CCEEEEEECCCCCEEEEEEEEEECHHHHCCEEEEEEEHHHHHHHHHHCEEEEEECCEEEE
GGETKEVLTPKNIKDVFGVECEIGENSKGHITINFLF
CCCCCEEECCCCCHHHHCCEEEECCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]