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The map label for this gene is pfk

Identifier: 18310167

GI number: 18310167

Start: 1380727

End: 1381827

Strand: Reverse

Name: pfk

Synonym: CPE1185

Alternate gene names: 18310167

Gene position: 1381827-1380727 (Counterclockwise)

Preceding gene: 18310168

Following gene: 18310166

Centisome position: 45.58

GC content: 32.15

Gene sequence:

>1101_bases
ATGATGCAACCAATTAAGAAAATAGCTATACTAACAGGAGGAGGAGATTGTCCTGGATTAAATGCAGTAATAAGAGCTGT
AACTAGAACTGCAATCTTAAAATATGGATATGAAGTTATAGGTTATAAATTTGGATATAGAGGTCTATATAATAATGACT
TTGTAAAATTGGATCTTGATTCTGTTTCTGGAATACTTCATAGAGGAGGAACAATACTTCATAGTTCAAATAAGGACAAC
CTATTTGATTATCAAGTTGAAGATGAGAATGGTAAAATAGTTAAAAAAGATGTTTCTGATGTTGGTGTTGAAAACTTAAA
GAAAGAAGGGGTAGATGCTCTAGTTGTTATTGGAGGAGATGGAACCTTAACCTCTGCAAGAGATTTCTCAAGAAAAGGTG
TAAATGTAATAGGTGTTCCTAAAACAATAGATAACGATTTATTAGCCACAGATGTAACCTTTGGATTTAATACTGCCACA
GAAATAGCTACAGAAGCTTTAGATAGACTTCATACTACAGCTGAGTCACATCATAGAATAATGCTTTTAGAAGTTATGGG
AAGAAACGCTGGTTGGATTGCTTTAGAATCAGGAATAGCTGGTTCTGCTGATGTAATATTATTACCTGAAATACCTTACG
ATATAAACAAGATAGTTGAAAAAGTAAAAGAAAGAGAAGAAGCCGGAAAACAGTTTACAATTATTGTAGTTGCAGAAGGA
GCAAAACCTAAAGATGGAGAAGTAGTTGTTTCTAAAATAGTTGATGATAGTCCAGACCCAATAAGATTAGGAGGAATAGC
TAACAAATTAGCTATTGACTTAGAAGGATTAATAAAAAATCATGAAATAAGAAGCACTGTTCTTGGACATATACAAAGAG
GGGGAAATACCTCTACTTATGATAGAATACTTTCCACTAAATATGGAGTTAAAGCTGTTGAACTTATAAATAGTAATTTA
TTTGGAAACATGGTTGCATTAAAAGGAAATAAAGTATCCTATGAAAGCTTAGAAAATGTAATTGGACATACTAAAAATGT
AGATCCAGAAGGTGAATTAGTAAACACTGCTAAAAGCATAGGAATTAGTTTCGCTGATTAA

Upstream 100 bases:

>100_bases
TACTTTCAAAAAAAAGCTTTATATGATATCATTTACTTAGAAGGTAATTGTAAAAAACTTACAGACTATGGTTTAAAATT
GTTTAGGAGGAGGATTTTAT

Downstream 100 bases:

>100_bases
TAATATAAAAACACTCATCATACTTTAATTTGATGAGTGTTTTCTTTTCTGTTTAGTAAAATTTCACTTATAAATTAGTA
AATTATAAGGATAAAGATGT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MMQPIKKIAILTGGGDCPGLNAVIRAVTRTAILKYGYEVIGYKFGYRGLYNNDFVKLDLDSVSGILHRGGTILHSSNKDN
LFDYQVEDENGKIVKKDVSDVGVENLKKEGVDALVVIGGDGTLTSARDFSRKGVNVIGVPKTIDNDLLATDVTFGFNTAT
EIATEALDRLHTTAESHHRIMLLEVMGRNAGWIALESGIAGSADVILLPEIPYDINKIVEKVKEREEAGKQFTIIVVAEG
AKPKDGEVVVSKIVDDSPDPIRLGGIANKLAIDLEGLIKNHEIRSTVLGHIQRGGNTSTYDRILSTKYGVKAVELINSNL
FGNMVALKGNKVSYESLENVIGHTKNVDPEGELVNTAKSIGISFAD

Sequences:

>Translated_366_residues
MMQPIKKIAILTGGGDCPGLNAVIRAVTRTAILKYGYEVIGYKFGYRGLYNNDFVKLDLDSVSGILHRGGTILHSSNKDN
LFDYQVEDENGKIVKKDVSDVGVENLKKEGVDALVVIGGDGTLTSARDFSRKGVNVIGVPKTIDNDLLATDVTFGFNTAT
EIATEALDRLHTTAESHHRIMLLEVMGRNAGWIALESGIAGSADVILLPEIPYDINKIVEKVKEREEAGKQFTIIVVAEG
AKPKDGEVVVSKIVDDSPDPIRLGGIANKLAIDLEGLIKNHEIRSTVLGHIQRGGNTSTYDRILSTKYGVKAVELINSNL
FGNMVALKGNKVSYESLENVIGHTKNVDPEGELVNTAKSIGISFAD
>Mature_366_residues
MMQPIKKIAILTGGGDCPGLNAVIRAVTRTAILKYGYEVIGYKFGYRGLYNNDFVKLDLDSVSGILHRGGTILHSSNKDN
LFDYQVEDENGKIVKKDVSDVGVENLKKEGVDALVVIGGDGTLTSARDFSRKGVNVIGVPKTIDNDLLATDVTFGFNTAT
EIATEALDRLHTTAESHHRIMLLEVMGRNAGWIALESGIAGSADVILLPEIPYDINKIVEKVKEREEAGKQFTIIVVAEG
AKPKDGEVVVSKIVDDSPDPIRLGGIANKLAIDLEGLIKNHEIRSTVLGHIQRGGNTSTYDRILSTKYGVKAVELINSNL
FGNMVALKGNKVSYESLENVIGHTKNVDPEGELVNTAKSIGISFAD

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI266453619, Length=373, Percent_Identity=32.9758713136729, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI266453768, Length=373, Percent_Identity=32.9758713136729, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI266453748, Length=373, Percent_Identity=32.9758713136729, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4505749, Length=373, Percent_Identity=32.9758713136729, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI48762920, Length=374, Percent_Identity=33.1550802139037, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI11321601, Length=394, Percent_Identity=32.994923857868, Blast_Score=171, Evalue=9e-43,
Organism=Escherichia coli, GI1790350, Length=356, Percent_Identity=38.2022471910112, Blast_Score=218, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI25147584, Length=336, Percent_Identity=31.547619047619, Blast_Score=160, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17558788, Length=394, Percent_Identity=27.1573604060914, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI72003601, Length=165, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323861, Length=378, Percent_Identity=33.3333333333333, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6321679, Length=343, Percent_Identity=34.6938775510204, Blast_Score=170, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24652337, Length=400, Percent_Identity=32, Blast_Score=184, Evalue=9e-47,
Organism=Drosophila melanogaster, GI28573326, Length=400, Percent_Identity=32.25, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17647809, Length=397, Percent_Identity=31.9899244332494, Blast_Score=182, Evalue=2e-46,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF2_CLOPE (Q8XL57)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_562101.1
- ProteinModelPortal:   Q8XL57
- SMR:   Q8XL57
- GeneID:   989494
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE1185
- NMPDR:   fig|195102.1.peg.1248
- HOGENOM:   HBG405044
- OMA:   MVALRGT
- PhylomeDB:   Q8XL57
- ProtClustDB:   CLSK810998
- BioCyc:   CPER195102:CPE1185-MONOMER
- BRENDA:   2.7.1.11
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02483

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 39515; Mature: 39515

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 144-144 BINDING 179-179 BINDING 284-284 BINDING 290-290 BINDING 293-293

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQPIKKIAILTGGGDCPGLNAVIRAVTRTAILKYGYEVIGYKFGYRGLYNNDFVKLDLD
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCCEEEEEHH
SVSGILHRGGTILHSSNKDNLFDYQVEDENGKIVKKDVSDVGVENLKKEGVDALVVIGGD
HHHHHHHCCCEEEECCCCCCEEEEEEECCCCCEEECCHHHCCHHHHHHCCCCEEEEECCC
GTLTSARDFSRKGVNVIGVPKTIDNDLLATDVTFGFNTATEIATEALDRLHTTAESHHRI
CCCCCCHHHHHCCCEEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEE
MLLEVMGRNAGWIALESGIAGSADVILLPEIPYDINKIVEKVKEREEAGKQFTIIVVAEG
EEEEEECCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECC
AKPKDGEVVVSKIVDDSPDPIRLGGIANKLAIDLEGLIKNHEIRSTVLGHIQRGGNTSTY
CCCCCCCEEEEEHHCCCCCCEEECCCCHHHEEEHHHHHCCCHHHHHHHHHHHCCCCCHHH
DRILSTKYGVKAVELINSNLFGNMVALKGNKVSYESLENVIGHTKNVDPEGELVNTAKSI
HHHHHHHCCCHHHHHHHCCCCCCEEEEECCEECHHHHHHHHCCCCCCCCCCHHHHHHHHC
GISFAD
CCEECC
>Mature Secondary Structure
MMQPIKKIAILTGGGDCPGLNAVIRAVTRTAILKYGYEVIGYKFGYRGLYNNDFVKLDLD
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCCEEEEEHH
SVSGILHRGGTILHSSNKDNLFDYQVEDENGKIVKKDVSDVGVENLKKEGVDALVVIGGD
HHHHHHHCCCEEEECCCCCCEEEEEEECCCCCEEECCHHHCCHHHHHHCCCCEEEEECCC
GTLTSARDFSRKGVNVIGVPKTIDNDLLATDVTFGFNTATEIATEALDRLHTTAESHHRI
CCCCCCHHHHHCCCEEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEE
MLLEVMGRNAGWIALESGIAGSADVILLPEIPYDINKIVEKVKEREEAGKQFTIIVVAEG
EEEEEECCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECC
AKPKDGEVVVSKIVDDSPDPIRLGGIANKLAIDLEGLIKNHEIRSTVLGHIQRGGNTSTY
CCCCCCCEEEEEHHCCCCCCEEECCCCHHHEEEHHHHHCCCHHHHHHHHHHHCCCCCHHH
DRILSTKYGVKAVELINSNLFGNMVALKGNKVSYESLENVIGHTKNVDPEGELVNTAKSI
HHHHHHHCCCHHHHHHHCCCCCCEEEEECCEECHHHHHHHHCCCCCCCCCCHHHHHHHHC
GISFAD
CCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842