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The map label for this gene is pflA [H]

Identifier: 18310135

GI number: 18310135

Start: 1347518

End: 1349752

Strand: Direct

Name: pflA [H]

Synonym: CPE1153

Alternate gene names: 18310135

Gene position: 1347518-1349752 (Clockwise)

Preceding gene: 18310134

Following gene: 149134307

Centisome position: 44.45

GC content: 33.56

Gene sequence:

>2235_bases
ATGTTTAAACAATGGGAAGGCTTTAAAGAAGGAGTATGGCAAAATGGAATAGATGTTAGAAACTTCATACAAAAGAACTA
TACTGCATATGAGGGAGACAAGAGTTTCTTAGAAGGAACAACAAAAAGAACTGATGCAGTTTTAGAAAAAGCTCAAAAGT
TAATTATAGAAGAAATAGAAAAAGGCATTATAGATGTTGCTACAGATAGAGTATCTGGAATAGACAATTATGAGCCTGGA
TATTTAGATAAAGATAACGAAGTTATCGTTGGTTTCCAAACAGATGCACCACTTAAAAGAATAGTTAATCCTTTCGGTGG
AATGAGAATGGTTGAAAGTTCTTTAAAAGAATACGGCTATGAATTAGATAGCAGTATAAGAGAACACTTTAATGCTTATA
GAAAAACTCATAACCAAGGTGTATTCGATGCATACTCAGCTGAAACAAGAGCTGCAAGATCTGCTGGTCTTTTAACTGGT
CTTCCAGATGCTTATGGTAGGGGAAGAATTATAGGTGACTATAGAAGAATTGCCCTTTACGGTATAGATTATCTAATAGA
AGAAAAGAAAAAAGATTATGCTAATATATCATTTGAAAGCGAAGGCTCAGTAGAGGAATATATTCAATTAAGAGAGAATG
TTTCAGAGCAAATAAGAGCTTTAGAAGCAATAAAATCAATGGCTGCAAGATATGGCTTTGATATATCTAAACCAGCTGCA
AACGCTAAGGAAGCAGTTCAATTCTTATACTTTGGATACTTAGCTGCAGTTAAAGAAAATAATGGAGCAGCAATGTCTCT
AGGAAGAACTTCTACTTTCTTAGATATTTTCATAGAAAGAGATCTTAAGGCAGGAACTTTAACTGAGTTAGAAGCTCAAG
AATTAATAGATCAATTTATAATAAAATTAAGATTAGTTAGACACTTAAGAACTCCTGAGTATAATGATTTATTTGCTGGA
GACCCAACTTGGGTAACAGAAGGTATTGGAGGTATGGGTGTAAATGGTAAACCATTAGTAACTAAAAACTCATTTAGATA
TCTTCAAACTTTAATAAACTTAGGACCTGCTCCAGAACCAAATATGACTGTTTTATGGTCAGATAGATTACCAGAAGGAT
TCAAAAAATTCTGTGCTGAGGTATCAATTAAAACTGACTCAATTCAATATGAAAATGATGAGTTAATGAGACCTATATAC
GGAGATGATTACTCAATAGCTTGTTGTGTATCAGCAATGCAAATTGGTAAGCAAATGCAATTCTTCGGAGCTAGAACTAA
CTTAGCTAAAGCTTTACTTTACACTATAAATGGTGGTATTGATGAAAAATCAGGTAAAGTTGTTATTAAAGGTTTAGAGC
CAATAACAGACGAAGTTTTAGATTATGATAAATTAAGAGCTAACTATGATAAAGTTTTAGAGTACGTTGCTAAGCTTTAT
GTAGATACAATGAATGTTATCCACTACATGCATGATAAATATGCTTATGAAGCTGGACAAATGGCTTTACATGATACTCA
TGTAGGAAGATTAATGGCATTTGGTATAGCTGGTTTATCAGTTGTAGCTGACTCATTAAGCGCAGTTAAATTCGCTAAAG
TTAAACCTATAAGAGAAAATGGAATAGCTGTAGACTTTGAAATCGAAGGTGACTTCCCTAAATACGGAAATGATGATGAT
AGAGTTGACGATTTAGCTGTAGAAATAGTTAGAAAGTTCTCAAGTGAGCTTAAGAAACATCCATTATATAGAGGTGCTAA
GCACACATTATCAATCCTAACTATTACTTCAAACGTAGTTTATGGTAAGAAAACAGGTTCAACTCCAGATGGAAGAAAAG
CTGGTGAAGCATTTGCTCCAGGAGCTAACCCAATGCATGGAAGAGATAACAGTGGAGCTTTAGCTAGCTTAAACTCAGTT
GCTAAAATTCCATACTGTGAAGTTTGTGAAGATGGTGTATCAAACACATTCTCAATCGTTCCAGATGCTTTAGGAAAAGA
AGAAGATTCTAGAATAGATAACTTAGTTGCTATTATGGATGGTTACTTCAAACAAGGAGCTCACCATCTAAATGTTAATG
TATTTAACAGAGAAACTTTAATGGACGCTATGGAGCATCCTGAAAAGTATCCAACATTAACAATCAGAGTATCAGGTTAT
GCTGTTAATTTCAATAGATTAACTAGAAATCAACAATTAGAGGTTATAAGCAGAACATTCCACGATTCTCTATAA

Upstream 100 bases:

>100_bases
AAAGTGTTGAAATTGATTCTCAAAAACTTTACAATGTGATGAAAATGTGTTAAATTTAGATTGATAAAGATTTTCAATTA
AGAAAGAGGGGGTAGACCAT

Downstream 100 bases:

>100_bases
TCTTATATAATAAGAGTGTATAAGGTTACTTAAAAAGCCTATAAATATATTCTGAAAATTACTAGATTTCACTAAGAATT
TATAGTTATTACTCCGAATA

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 744; Mature: 744

Protein sequence:

>744_residues
MFKQWEGFKEGVWQNGIDVRNFIQKNYTAYEGDKSFLEGTTKRTDAVLEKAQKLIIEEIEKGIIDVATDRVSGIDNYEPG
YLDKDNEVIVGFQTDAPLKRIVNPFGGMRMVESSLKEYGYELDSSIREHFNAYRKTHNQGVFDAYSAETRAARSAGLLTG
LPDAYGRGRIIGDYRRIALYGIDYLIEEKKKDYANISFESEGSVEEYIQLRENVSEQIRALEAIKSMAARYGFDISKPAA
NAKEAVQFLYFGYLAAVKENNGAAMSLGRTSTFLDIFIERDLKAGTLTELEAQELIDQFIIKLRLVRHLRTPEYNDLFAG
DPTWVTEGIGGMGVNGKPLVTKNSFRYLQTLINLGPAPEPNMTVLWSDRLPEGFKKFCAEVSIKTDSIQYENDELMRPIY
GDDYSIACCVSAMQIGKQMQFFGARTNLAKALLYTINGGIDEKSGKVVIKGLEPITDEVLDYDKLRANYDKVLEYVAKLY
VDTMNVIHYMHDKYAYEAGQMALHDTHVGRLMAFGIAGLSVVADSLSAVKFAKVKPIRENGIAVDFEIEGDFPKYGNDDD
RVDDLAVEIVRKFSSELKKHPLYRGAKHTLSILTITSNVVYGKKTGSTPDGRKAGEAFAPGANPMHGRDNSGALASLNSV
AKIPYCEVCEDGVSNTFSIVPDALGKEEDSRIDNLVAIMDGYFKQGAHHLNVNVFNRETLMDAMEHPEKYPTLTIRVSGY
AVNFNRLTRNQQLEVISRTFHDSL

Sequences:

>Translated_744_residues
MFKQWEGFKEGVWQNGIDVRNFIQKNYTAYEGDKSFLEGTTKRTDAVLEKAQKLIIEEIEKGIIDVATDRVSGIDNYEPG
YLDKDNEVIVGFQTDAPLKRIVNPFGGMRMVESSLKEYGYELDSSIREHFNAYRKTHNQGVFDAYSAETRAARSAGLLTG
LPDAYGRGRIIGDYRRIALYGIDYLIEEKKKDYANISFESEGSVEEYIQLRENVSEQIRALEAIKSMAARYGFDISKPAA
NAKEAVQFLYFGYLAAVKENNGAAMSLGRTSTFLDIFIERDLKAGTLTELEAQELIDQFIIKLRLVRHLRTPEYNDLFAG
DPTWVTEGIGGMGVNGKPLVTKNSFRYLQTLINLGPAPEPNMTVLWSDRLPEGFKKFCAEVSIKTDSIQYENDELMRPIY
GDDYSIACCVSAMQIGKQMQFFGARTNLAKALLYTINGGIDEKSGKVVIKGLEPITDEVLDYDKLRANYDKVLEYVAKLY
VDTMNVIHYMHDKYAYEAGQMALHDTHVGRLMAFGIAGLSVVADSLSAVKFAKVKPIRENGIAVDFEIEGDFPKYGNDDD
RVDDLAVEIVRKFSSELKKHPLYRGAKHTLSILTITSNVVYGKKTGSTPDGRKAGEAFAPGANPMHGRDNSGALASLNSV
AKIPYCEVCEDGVSNTFSIVPDALGKEEDSRIDNLVAIMDGYFKQGAHHLNVNVFNRETLMDAMEHPEKYPTLTIRVSGY
AVNFNRLTRNQQLEVISRTFHDSL
>Mature_744_residues
MFKQWEGFKEGVWQNGIDVRNFIQKNYTAYEGDKSFLEGTTKRTDAVLEKAQKLIIEEIEKGIIDVATDRVSGIDNYEPG
YLDKDNEVIVGFQTDAPLKRIVNPFGGMRMVESSLKEYGYELDSSIREHFNAYRKTHNQGVFDAYSAETRAARSAGLLTG
LPDAYGRGRIIGDYRRIALYGIDYLIEEKKKDYANISFESEGSVEEYIQLRENVSEQIRALEAIKSMAARYGFDISKPAA
NAKEAVQFLYFGYLAAVKENNGAAMSLGRTSTFLDIFIERDLKAGTLTELEAQELIDQFIIKLRLVRHLRTPEYNDLFAG
DPTWVTEGIGGMGVNGKPLVTKNSFRYLQTLINLGPAPEPNMTVLWSDRLPEGFKKFCAEVSIKTDSIQYENDELMRPIY
GDDYSIACCVSAMQIGKQMQFFGARTNLAKALLYTINGGIDEKSGKVVIKGLEPITDEVLDYDKLRANYDKVLEYVAKLY
VDTMNVIHYMHDKYAYEAGQMALHDTHVGRLMAFGIAGLSVVADSLSAVKFAKVKPIRENGIAVDFEIEGDFPKYGNDDD
RVDDLAVEIVRKFSSELKKHPLYRGAKHTLSILTITSNVVYGKKTGSTPDGRKAGEAFAPGANPMHGRDNSGALASLNSV
AKIPYCEVCEDGVSNTFSIVPDALGKEEDSRIDNLVAIMDGYFKQGAHHLNVNVFNRETLMDAMEHPEKYPTLTIRVSGY
AVNFNRLTRNQQLEVISRTFHDSL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=750, Percent_Identity=60.9333333333333, Blast_Score=951, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=750, Percent_Identity=60.6666666666667, Blast_Score=927, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=607, Percent_Identity=27.6771004942339, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1790388, Length=726, Percent_Identity=25.068870523416, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788933, Length=60, Percent_Identity=70, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 83323; Mature: 83323

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKQWEGFKEGVWQNGIDVRNFIQKNYTAYEGDKSFLEGTTKRTDAVLEKAQKLIIEEIE
CCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHH
KGIIDVATDRVSGIDNYEPGYLDKDNEVIVGFQTDAPLKRIVNPFGGMRMVESSLKEYGY
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHCC
ELDSSIREHFNAYRKTHNQGVFDAYSAETRAARSAGLLTGLPDAYGRGRIIGDYRRIALY
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHH
GIDYLIEEKKKDYANISFESEGSVEEYIQLRENVSEQIRALEAIKSMAARYGFDISKPAA
HHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NAKEAVQFLYFGYLAAVKENNGAAMSLGRTSTFLDIFIERDLKAGTLTELEAQELIDQFI
CHHHHHHHHHHHHHHHEECCCCCEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IKLRLVRHLRTPEYNDLFAGDPTWVTEGIGGMGVNGKPLVTKNSFRYLQTLINLGPAPEP
HHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCC
NMTVLWSDRLPEGFKKFCAEVSIKTDSIQYENDELMRPIYGDDYSIACCVSAMQIGKQMQ
CEEEEECCCCCHHHHHHHHHCEEECCCEEECCCHHCCCCCCCCCHHHHHHHHHHHHHHHH
FFGARTNLAKALLYTINGGIDEKSGKVVIKGLEPITDEVLDYDKLRANYDKVLEYVAKLY
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
VDTMNVIHYMHDKYAYEAGQMALHDTHVGRLMAFGIAGLSVVADSLSAVKFAKVKPIREN
HHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GIAVDFEIEGDFPKYGNDDDRVDDLAVEIVRKFSSELKKHPLYRGAKHTLSILTITSNVV
CEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCEE
YGKKTGSTPDGRKAGEAFAPGANPMHGRDNSGALASLNSVAKIPYCEVCEDGVSNTFSIV
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCHHHC
PDALGKEEDSRIDNLVAIMDGYFKQGAHHLNVNVFNRETLMDAMEHPEKYPTLTIRVSGY
CHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHCCCCCCEEEEEEEEE
AVNFNRLTRNQQLEVISRTFHDSL
EEEHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKQWEGFKEGVWQNGIDVRNFIQKNYTAYEGDKSFLEGTTKRTDAVLEKAQKLIIEEIE
CCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHH
KGIIDVATDRVSGIDNYEPGYLDKDNEVIVGFQTDAPLKRIVNPFGGMRMVESSLKEYGY
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHCC
ELDSSIREHFNAYRKTHNQGVFDAYSAETRAARSAGLLTGLPDAYGRGRIIGDYRRIALY
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHH
GIDYLIEEKKKDYANISFESEGSVEEYIQLRENVSEQIRALEAIKSMAARYGFDISKPAA
HHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NAKEAVQFLYFGYLAAVKENNGAAMSLGRTSTFLDIFIERDLKAGTLTELEAQELIDQFI
CHHHHHHHHHHHHHHHEECCCCCEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IKLRLVRHLRTPEYNDLFAGDPTWVTEGIGGMGVNGKPLVTKNSFRYLQTLINLGPAPEP
HHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCC
NMTVLWSDRLPEGFKKFCAEVSIKTDSIQYENDELMRPIYGDDYSIACCVSAMQIGKQMQ
CEEEEECCCCCHHHHHHHHHCEEECCCEEECCCHHCCCCCCCCCHHHHHHHHHHHHHHHH
FFGARTNLAKALLYTINGGIDEKSGKVVIKGLEPITDEVLDYDKLRANYDKVLEYVAKLY
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
VDTMNVIHYMHDKYAYEAGQMALHDTHVGRLMAFGIAGLSVVADSLSAVKFAKVKPIREN
HHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GIAVDFEIEGDFPKYGNDDDRVDDLAVEIVRKFSSELKKHPLYRGAKHTLSILTITSNVV
CEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCEE
YGKKTGSTPDGRKAGEAFAPGANPMHGRDNSGALASLNSVAKIPYCEVCEDGVSNTFSIV
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCHHHC
PDALGKEEDSRIDNLVAIMDGYFKQGAHHLNVNVFNRETLMDAMEHPEKYPTLTIRVSGY
CHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHCCCCCCEEEEEEEEE
AVNFNRLTRNQQLEVISRTFHDSL
EEEHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]