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The map label for this gene is pflA [H]

Identifier: 149134307

GI number: 149134307

Start: 1350038

End: 1350745

Strand: Direct

Name: pflA [H]

Synonym: CPE1154

Alternate gene names: 149134307

Gene position: 1350038-1350745 (Clockwise)

Preceding gene: 18310135

Following gene: 18310137

Centisome position: 44.53

GC content: 31.07

Gene sequence:

>708_bases
ATGGTTAAAGGAAGAATTCATTCATTAGAAACTATGGGCTTAGTAGATGGACCTGGTATAAGATTCGTAGTTTTTATGCA
AGGCTGTGGAATAAGATGTGCTTTTTGTCATAATCCAGATACTTGGTGTAAAGATAAGGGGACTGAATATACTCCAGAGG
AGCTTGTAAATAAGATAAAAAGATTTAAAACATATTTTAATGCATCAGGTGGTGGTGTTACTTTCTCAGGTGGAGAGCCT
CTTCTTCAACCAGAATTTTTACTAGAGTGTTTAAAGTTATGTAAAAAGGAAGGAATACATACCACATTAGATACTGCAGG
TGTTGGATTAGGAAATTACGAAGAAATTCTTGAATATGTTGACCTTATATTATTTGATGTAAAGGAAACTGATCCTGAGA
AATATAAGAGTTTAGTAGGAGTTCCAATAGATAAATCCTTAGAGTTTCTAAAGGTAACTCAGTCCATGAATAAAAAAATG
TGGATAAGACATGTTGTAGTTCCTGGATATACAGATAATAAAGAAGATTTAATGAGAATTAAGAATTTTGTAGATGGTCT
TAATAATATAGAAAAGGTTGAACTTTTACCTTATCATGTTTTAGGTGTAAATAAATATGAAGGGCTTAATATTCCATATA
GATTAGAGGGAGTTCCACCTTTAGATAAAAAGTGGTTAAAAGAACTTGAAAAAGAGATATTTAATTAA

Upstream 100 bases:

>100_bases
AAGTTCATCTAGATATTTTCTTCATTATATTTATAGGGTTAATTAAGTTAAGACCTTGTACACTCTTTTTTGAAATGTTT
ACTGTAAGGAGGACAACATT

Downstream 100 bases:

>100_bases
TATAATTATTTAGATAAAAACTAAAGTCTAATTTTAGGCTTTAGTTTTTTCTTACATAAAAAATATGTTAGAATAATTAG
TATGACATAATTAACATTTT

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MVKGRIHSLETMGLVDGPGIRFVVFMQGCGIRCAFCHNPDTWCKDKGTEYTPEELVNKIKRFKTYFNASGGGVTFSGGEP
LLQPEFLLECLKLCKKEGIHTTLDTAGVGLGNYEEILEYVDLILFDVKETDPEKYKSLVGVPIDKSLEFLKVTQSMNKKM
WIRHVVVPGYTDNKEDLMRIKNFVDGLNNIEKVELLPYHVLGVNKYEGLNIPYRLEGVPPLDKKWLKELEKEIFN

Sequences:

>Translated_235_residues
MVKGRIHSLETMGLVDGPGIRFVVFMQGCGIRCAFCHNPDTWCKDKGTEYTPEELVNKIKRFKTYFNASGGGVTFSGGEP
LLQPEFLLECLKLCKKEGIHTTLDTAGVGLGNYEEILEYVDLILFDVKETDPEKYKSLVGVPIDKSLEFLKVTQSMNKKM
WIRHVVVPGYTDNKEDLMRIKNFVDGLNNIEKVELLPYHVLGVNKYEGLNIPYRLEGVPPLDKKWLKELEKEIFN
>Mature_235_residues
MVKGRIHSLETMGLVDGPGIRFVVFMQGCGIRCAFCHNPDTWCKDKGTEYTPEELVNKIKRFKTYFNASGGGVTFSGGEP
LLQPEFLLECLKLCKKEGIHTTLDTAGVGLGNYEEILEYVDLILFDVKETDPEKYKSLVGVPIDKSLEFLKVTQSMNKKM
WIRHVVVPGYTDNKEDLMRIKNFVDGLNNIEKVELLPYHVLGVNKYEGLNIPYRLEGVPPLDKKWLKELEKEIFN

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=233, Percent_Identity=46.7811158798283, Blast_Score=224, Evalue=3e-60,
Organism=Escherichia coli, GI1790389, Length=247, Percent_Identity=27.9352226720648, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI226510931, Length=162, Percent_Identity=33.9506172839506, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 26780; Mature: 26780

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKGRIHSLETMGLVDGPGIRFVVFMQGCGIRCAFCHNPDTWCKDKGTEYTPEELVNKIK
CCCCCCCCHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
RFKTYFNASGGGVTFSGGEPLLQPEFLLECLKLCKKEGIHTTLDTAGVGLGNYEEILEYV
HHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEHHHCCCCCCCHHHHHHHH
DLILFDVKETDPEKYKSLVGVPIDKSLEFLKVTQSMNKKMWIRHVVVPGYTDNKEDLMRI
HHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCHHEEEEEEEECCCCCCHHHHHHH
KNFVDGLNNIEKVELLPYHVLGVNKYEGLNIPYRLEGVPPLDKKWLKELEKEIFN
HHHHHHHCCCCCEEECEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVKGRIHSLETMGLVDGPGIRFVVFMQGCGIRCAFCHNPDTWCKDKGTEYTPEELVNKIK
CCCCCCCCHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
RFKTYFNASGGGVTFSGGEPLLQPEFLLECLKLCKKEGIHTTLDTAGVGLGNYEEILEYV
HHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEHHHCCCCCCCHHHHHHHH
DLILFDVKETDPEKYKSLVGVPIDKSLEFLKVTQSMNKKMWIRHVVVPGYTDNKEDLMRI
HHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCHHEEEEEEEECCCCCCHHHHHHH
KNFVDGLNNIEKVELLPYHVLGVNKYEGLNIPYRLEGVPPLDKKWLKELEKEIFN
HHHHHHHCCCCCEEECEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]