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The map label for this gene is cobS [H]

Identifier: 18310018

GI number: 18310018

Start: 1237835

End: 1238590

Strand: Direct

Name: cobS [H]

Synonym: CPE1036

Alternate gene names: 18310018

Gene position: 1237835-1238590 (Clockwise)

Preceding gene: 18310017

Following gene: 18310019

Centisome position: 40.83

GC content: 28.17

Gene sequence:

>756_bases
ATGAAAATTTTTTATAAGGCTATAAATATGACCTTAAGCATGTTTACAGTTATTCCATCGCCTAAATATGAATGGGATGA
CAGGGCTGCAAAACATATAATGAAGCTTTATCCTTTTATAGGGTTAATTATAGGAGCACTTTGGTATTTAAGTTTTTTTG
TATTAAGTAAGCTAAATGTACCAATTATGCTTATGGCAGCTCTTATTTTAACGGTGCCATATATTTTAACTGGATTCTTA
CATCTAGATGGTTTTATGGATGTTTCTGATGCACTTCTTTCTAGAAGAGATAAGGAGACAAAACTTAGAATTTTAAAGGA
TTCTACAGTTGGAGCTTTTTCAGTTATTTCTGTAGTTTTATTACTACTAGTAGAATTTGCTGGAATGTTTACTGTTTTAA
ATAAAAATTTAGATATGAGAGTATTAATATTTATACCAATAGCATCAAGGGCAATAAATGGATACTTTATTGTAAGTCAA
GAGATGCTTGGACAAAGTTCTCTAGCAAAATTTTTTAAAGAAGGAACTGGAAAAGTAGATGAAATAATTTTACTAGGAAT
ATATGTATTAGTTGCATTAATAACGTTTTTCACCTTAGGAATAAATTATTTAATAGCTATTTTAGCAATGGGACTCATTT
CTTTTATTTTACTTTTAAAAGTAAAAAAAGAATTAGGAGGAATAAATGGAGACGTTGCAGGATATATTCTTGTTCTTATG
GAATTTACAGGGATTTTACTTTTAGGAATTATTTAA

Upstream 100 bases:

>100_bases
TTAGGTAAAATAAATTGTGAATTAGCTTCCTTCTCAGATGTGGTTATAGAAGCTTCCTTTGGAAACTTAATATTTCATAA
AGGAGAGGAGGTTTTAGAAG

Downstream 100 bases:

>100_bases
GGAGGAGAAGCTTTTGAAACTTTTATTTGGCGGTGCCTATAATGGAAAACTAAGGTATGTAAGAGAAAAGTTAGGCATAA
ATGAAGAGGAAATATTTTAT

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKIFYKAINMTLSMFTVIPSPKYEWDDRAAKHIMKLYPFIGLIIGALWYLSFFVLSKLNVPIMLMAALILTVPYILTGFL
HLDGFMDVSDALLSRRDKETKLRILKDSTVGAFSVISVVLLLLVEFAGMFTVLNKNLDMRVLIFIPIASRAINGYFIVSQ
EMLGQSSLAKFFKEGTGKVDEIILLGIYVLVALITFFTLGINYLIAILAMGLISFILLLKVKKELGGINGDVAGYILVLM
EFTGILLLGII

Sequences:

>Translated_251_residues
MKIFYKAINMTLSMFTVIPSPKYEWDDRAAKHIMKLYPFIGLIIGALWYLSFFVLSKLNVPIMLMAALILTVPYILTGFL
HLDGFMDVSDALLSRRDKETKLRILKDSTVGAFSVISVVLLLLVEFAGMFTVLNKNLDMRVLIFIPIASRAINGYFIVSQ
EMLGQSSLAKFFKEGTGKVDEIILLGIYVLVALITFFTLGINYLIAILAMGLISFILLLKVKKELGGINGDVAGYILVLM
EFTGILLLGII
>Mature_251_residues
MKIFYKAINMTLSMFTVIPSPKYEWDDRAAKHIMKLYPFIGLIIGALWYLSFFVLSKLNVPIMLMAALILTVPYILTGFL
HLDGFMDVSDALLSRRDKETKLRILKDSTVGAFSVISVVLLLLVEFAGMFTVLNKNLDMRVLIFIPIASRAINGYFIVSQ
EMLGQSSLAKFFKEGTGKVDEIILLGIYVLVALITFFTLGINYLIAILAMGLISFILLLKVKKELGGINGDVAGYILVLM
EFTGILLLGII

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=243, Percent_Identity=27.9835390946502, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27977; Mature: 27977

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFYKAINMTLSMFTVIPSPKYEWDDRAAKHIMKLYPFIGLIIGALWYLSFFVLSKLNV
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PIMLMAALILTVPYILTGFLHLDGFMDVSDALLSRRDKETKLRILKDSTVGAFSVISVVL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
LLLVEFAGMFTVLNKNLDMRVLIFIPIASRAINGYFIVSQEMLGQSSLAKFFKEGTGKVD
HHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHCCEEEEEHHHHCHHHHHHHHHHCCCCHH
EIILLGIYVLVALITFFTLGINYLIAILAMGLISFILLLKVKKELGGINGDVAGYILVLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EFTGILLLGII
HHHHHHHHHCC
>Mature Secondary Structure
MKIFYKAINMTLSMFTVIPSPKYEWDDRAAKHIMKLYPFIGLIIGALWYLSFFVLSKLNV
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PIMLMAALILTVPYILTGFLHLDGFMDVSDALLSRRDKETKLRILKDSTVGAFSVISVVL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
LLLVEFAGMFTVLNKNLDMRVLIFIPIASRAINGYFIVSQEMLGQSSLAKFFKEGTGKVD
HHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHCCEEEEEHHHHCHHHHHHHHHHCCCCHH
EIILLGIYVLVALITFFTLGINYLIAILAMGLISFILLLKVKKELGGINGDVAGYILVLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EFTGILLLGII
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA