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The map label for this gene is bgaL [H]

Identifier: 18309813

GI number: 18309813

Start: 1015357

End: 1017771

Strand: Direct

Name: bgaL [H]

Synonym: CPE0831

Alternate gene names: 18309813

Gene position: 1015357-1017771 (Clockwise)

Preceding gene: 18309811

Following gene: 18309814

Centisome position: 33.49

GC content: 29.19

Gene sequence:

>2415_bases
ATGAGGAAAATAATTCATATCAATGATCAATGGGTTTATGCAAATGATTACAAAAGTGAGTATTTAAAAAATGAGTTTGA
TTTTAGTAATTTTGAAAGGGTAGATTTACCTCATACAAATATAGAGTTACCATATAATTATTTTGATGAAAAAGCATATC
AATTTGTTTCTACTTATGTTAAAACTTTAAAATTTGATGAGAATATTAAAGGGAAAAAAGTATTTTTAGATTTTGAAGGA
GTTATGATAGCCGCAGAAGTATATTTAAATGGAATTCATGTTGGTGGACATAAGGGGGGGTACACTAATTTTTCAATAGA
TATTACTGATGCTTTAAAAATTAATGAAGATAATATATTAAAAGTTGTTGTTGACTCAACAGAAAGACCGGATATACCTC
CTCATGGATATGTTGTTGATTATTTAACCTATGGAGGAATATATAGAGAAGTTTCCTTAAGAGTAGTTGAACCTATATTT
ATAAATAATTTATATGCAAGGGCATATGATTGTTTAAAGGAAGAAAAAAGATTAGAACTTGATATAGAAATAAATAATTT
TGAAAAATATAGAGATGATTTAGAAATTGTTGTAGAGTTTGGTGATGATACTTTTGAAGAAACTTTAAGTACAAAGTTAC
CAATTGAAGAAGGAACAAGTATTAAAAACATAGAAATAGACCAATTAAATATGGTTAAGTTATGGGATATAGAAAATCCT
AAGCTTTATGAAATAAAAGTTAAGTTATTAAAAGGTTCAGAAGTTATAGATGAATATAAAGATACTTTTGGATTTAGAGA
GGCTGAATTTAGATCAGATGGTTTTTATTTAAATGGAAGAAGAGTTAAACTTGTTGGATTAAACCGTCATCAGGCTTATC
CATATGTAGGATATGCTATGCCTCAAAGGGTTCAAGAAAAGGATGCTGAGATTTTAAAATATGAATTAGGACTTAATATA
GTTAGAACATCTCACTATCCGCAATCAATATATTTCCTAAGAAAATGTGATGAGATTGGACTATTAGTTTTTGAAGAGAT
ACCTGGTTGGCAACATATAGGTGATGAAGCTTGGCAAGCAGAATCTATTAAAAATGTAGAGGAAATGATAAAAAGAGATT
ACAATAGACCTTCCATAGTTTTATGGGGCGTTAGAATAAATGAGTCTCAAGATAGTCATGATTTTTATGTGAAAACTAAT
GCTATGGCAAAGAGTTTAGATCCTATTAGACAAACTGGTGGGGTTAGATACTTAGAAAATAGTGATTTCCTAGAAGATGT
TTATACCATGAATGATTTTATACACAGTGGGGGAGAAAAAGTATTAAGAACTCAAAGTGAAGTAACAGGACAAGTAGATA
AAGTTCCTTATTTAGTAACTGAGTATAATGGGCATATGTATCCAACAAAAAGCTTTGATCAAGAATGTAAAAAAGTTGAA
CATGCTTATAGACATTTGAGAGTTATTAATGAATCCTTTGGCTTAGATGAAATAAGTGGAGCCATAGGATGGTGTGCTTT
TGATTATAATACACATAGTTCCTTTGGTTCAGGAGATAAAATTTGTTACCATGGAGTTTCTGATATGTTCAGAAATCCTA
AGTATGCAGCTTATTCATATGCTAGCCAAAAGAAAGTAGAAGATGGTGTGGTTTTAGAGCCTATTACTTTAGGAGCTAAG
GGAGAAAGGGATGGAGGAGCAATACTTCCATTTACAGTTCTTACAAACTGTGATTATATAAAAATATTTAAAGATGGAAT
ATATATAGATACTTATTATCCTAATAAAGAAAAGTTCCCTAATTTACCACATCCACCAATAGAGGTTTCACATATTTTAT
CTATGGATTCAGAAATACCTCTTACTGAAGAAGCAAAAAAAGAAATTAAAGACTTTGTATTAAATAAATTAAAAGATTCT
AATTTAACTAATTTAGCTGAAGAAGATTTTAAATATATTGAAGAATTTAGTGAAAGAGTAAATATACCTGTATTTAAAAT
AATGTCTTTAGTTTATAAATTAGCTGGAGGTTGGGGAGATAAGGAAAACTCTTTAATAATAAAAGGCTTTATAGATAATA
AAGAGGTTGCTTCAAAAGAAATAGGTGAGCTTAGAAGCATGAATAAGTTAGAAGTTACACCAGATGATTTAGAACTTTCA
TTAGATAAAATAAGTTATGATGCTACTAGAATCGTGGTTAAACTTTTAGATAATTTAGGAGAGGTTCTTTTCTTAAATAA
TGATTTTATTGAAGTAGAAATAGATGGACCTTTAAGTATAATGGGACCAAGTAAGTTTGGAATTTCTGGTGGAGCAGTCG
CTTTCTGGGTAAGAACTCAAGGGAAAAAAGGTCTTTGCAAAATAAAAGTTAAGAGCATGTATTTTGAAGAAGAAATTTCT
ATAGAAGTTAAGTAG

Upstream 100 bases:

>100_bases
ATTGTTAAAAGAAAGTTCATAAGAAAACATGTATAATGTATATATGGTAATAATTAACTCAAGGTTTATATAATTAATCG
TAAAATTAGGAGGAGAATTT

Downstream 100 bases:

>100_bases
AGTTAACTTATTAGTATTTTTATAGGGAGACTTAAGTAATTTTAAATTAAAGAAATAGATTATGTTTTAAAGTGAAGTTT
TGCTTTAGATTAATTTAGAA

Product: beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 804; Mature: 804

Protein sequence:

>804_residues
MRKIIHINDQWVYANDYKSEYLKNEFDFSNFERVDLPHTNIELPYNYFDEKAYQFVSTYVKTLKFDENIKGKKVFLDFEG
VMIAAEVYLNGIHVGGHKGGYTNFSIDITDALKINEDNILKVVVDSTERPDIPPHGYVVDYLTYGGIYREVSLRVVEPIF
INNLYARAYDCLKEEKRLELDIEINNFEKYRDDLEIVVEFGDDTFEETLSTKLPIEEGTSIKNIEIDQLNMVKLWDIENP
KLYEIKVKLLKGSEVIDEYKDTFGFREAEFRSDGFYLNGRRVKLVGLNRHQAYPYVGYAMPQRVQEKDAEILKYELGLNI
VRTSHYPQSIYFLRKCDEIGLLVFEEIPGWQHIGDEAWQAESIKNVEEMIKRDYNRPSIVLWGVRINESQDSHDFYVKTN
AMAKSLDPIRQTGGVRYLENSDFLEDVYTMNDFIHSGGEKVLRTQSEVTGQVDKVPYLVTEYNGHMYPTKSFDQECKKVE
HAYRHLRVINESFGLDEISGAIGWCAFDYNTHSSFGSGDKICYHGVSDMFRNPKYAAYSYASQKKVEDGVVLEPITLGAK
GERDGGAILPFTVLTNCDYIKIFKDGIYIDTYYPNKEKFPNLPHPPIEVSHILSMDSEIPLTEEAKKEIKDFVLNKLKDS
NLTNLAEEDFKYIEEFSERVNIPVFKIMSLVYKLAGGWGDKENSLIIKGFIDNKEVASKEIGELRSMNKLEVTPDDLELS
LDKISYDATRIVVKLLDNLGEVLFLNNDFIEVEIDGPLSIMGPSKFGISGGAVAFWVRTQGKKGLCKIKVKSMYFEEEIS
IEVK

Sequences:

>Translated_804_residues
MRKIIHINDQWVYANDYKSEYLKNEFDFSNFERVDLPHTNIELPYNYFDEKAYQFVSTYVKTLKFDENIKGKKVFLDFEG
VMIAAEVYLNGIHVGGHKGGYTNFSIDITDALKINEDNILKVVVDSTERPDIPPHGYVVDYLTYGGIYREVSLRVVEPIF
INNLYARAYDCLKEEKRLELDIEINNFEKYRDDLEIVVEFGDDTFEETLSTKLPIEEGTSIKNIEIDQLNMVKLWDIENP
KLYEIKVKLLKGSEVIDEYKDTFGFREAEFRSDGFYLNGRRVKLVGLNRHQAYPYVGYAMPQRVQEKDAEILKYELGLNI
VRTSHYPQSIYFLRKCDEIGLLVFEEIPGWQHIGDEAWQAESIKNVEEMIKRDYNRPSIVLWGVRINESQDSHDFYVKTN
AMAKSLDPIRQTGGVRYLENSDFLEDVYTMNDFIHSGGEKVLRTQSEVTGQVDKVPYLVTEYNGHMYPTKSFDQECKKVE
HAYRHLRVINESFGLDEISGAIGWCAFDYNTHSSFGSGDKICYHGVSDMFRNPKYAAYSYASQKKVEDGVVLEPITLGAK
GERDGGAILPFTVLTNCDYIKIFKDGIYIDTYYPNKEKFPNLPHPPIEVSHILSMDSEIPLTEEAKKEIKDFVLNKLKDS
NLTNLAEEDFKYIEEFSERVNIPVFKIMSLVYKLAGGWGDKENSLIIKGFIDNKEVASKEIGELRSMNKLEVTPDDLELS
LDKISYDATRIVVKLLDNLGEVLFLNNDFIEVEIDGPLSIMGPSKFGISGGAVAFWVRTQGKKGLCKIKVKSMYFEEEIS
IEVK
>Mature_804_residues
MRKIIHINDQWVYANDYKSEYLKNEFDFSNFERVDLPHTNIELPYNYFDEKAYQFVSTYVKTLKFDENIKGKKVFLDFEG
VMIAAEVYLNGIHVGGHKGGYTNFSIDITDALKINEDNILKVVVDSTERPDIPPHGYVVDYLTYGGIYREVSLRVVEPIF
INNLYARAYDCLKEEKRLELDIEINNFEKYRDDLEIVVEFGDDTFEETLSTKLPIEEGTSIKNIEIDQLNMVKLWDIENP
KLYEIKVKLLKGSEVIDEYKDTFGFREAEFRSDGFYLNGRRVKLVGLNRHQAYPYVGYAMPQRVQEKDAEILKYELGLNI
VRTSHYPQSIYFLRKCDEIGLLVFEEIPGWQHIGDEAWQAESIKNVEEMIKRDYNRPSIVLWGVRINESQDSHDFYVKTN
AMAKSLDPIRQTGGVRYLENSDFLEDVYTMNDFIHSGGEKVLRTQSEVTGQVDKVPYLVTEYNGHMYPTKSFDQECKKVE
HAYRHLRVINESFGLDEISGAIGWCAFDYNTHSSFGSGDKICYHGVSDMFRNPKYAAYSYASQKKVEDGVVLEPITLGAK
GERDGGAILPFTVLTNCDYIKIFKDGIYIDTYYPNKEKFPNLPHPPIEVSHILSMDSEIPLTEEAKKEIKDFVLNKLKDS
NLTNLAEEDFKYIEEFSERVNIPVFKIMSLVYKLAGGWGDKENSLIIKGFIDNKEVASKEIGELRSMNKLEVTPDDLELS
LDKISYDATRIVVKLLDNLGEVLFLNNDFIEVEIDGPLSIMGPSKFGISGGAVAFWVRTQGKKGLCKIKVKSMYFEEEIS
IEVK

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=536, Percent_Identity=25.9328358208955, Blast_Score=100, Evalue=8e-21,
Organism=Escherichia coli, GI48994920, Length=407, Percent_Identity=28.5012285012285, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787903, Length=565, Percent_Identity=23.0088495575221, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1786539, Length=400, Percent_Identity=25.75, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17510825, Length=371, Percent_Identity=25.3369272237197, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI62471735, Length=526, Percent_Identity=24.7148288973384, Blast_Score=120, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19922582, Length=526, Percent_Identity=24.7148288973384, Blast_Score=120, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24655438, Length=526, Percent_Identity=24.7148288973384, Blast_Score=120, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24651745, Length=528, Percent_Identity=23.6742424242424, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 92619; Mature: 92619

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00719 GLYCOSYL_HYDROL_F2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIIHINDQWVYANDYKSEYLKNEFDFSNFERVDLPHTNIELPYNYFDEKAYQFVSTYV
CCEEEEECCCEEEECCHHHHHHCCCCCCCCCEEECCCCCCEECCHHHHHHHHHHHHHHHH
KTLKFDENIKGKKVFLDFEGVMIAAEVYLNGIHVGGHKGGYTNFSIDITDALKINEDNIL
HHHCCCCCCCCCEEEEEECCEEEEEEEEEEEEEECCCCCCCEEEEEEEEEEEEECCCCEE
KVVVDSTERPDIPPHGYVVDYLTYGGIYREVSLRVVEPIFINNLYARAYDCLKEEKRLEL
EEEECCCCCCCCCCCCEEEEEHHHCCEEEEEEEEEECHHHHHHHHHHHHHHHHHCCCEEE
DIEINNFEKYRDDLEIVVEFGDDTFEETLSTKLPIEEGTSIKNIEIDQLNMVKLWDIENP
EEEECCHHHHCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEECCCC
KLYEIKVKLLKGSEVIDEYKDTFGFREAEFRSDGFYLNGRRVKLVGLNRHQAYPYVGYAM
EEEEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCEECCCC
PQRVQEKDAEILKYELGLNIVRTSHYPQSIYFLRKCDEIGLLVFEEIPGWQHIGDEAWQA
HHHHHHHHHHHEEHCCCCEEEEECCCCCHHHHHHHCCCCCEEEEECCCCHHHCCCHHHHH
ESIKNVEEMIKRDYNRPSIVLWGVRINESQDSHDFYVKTNAMAKSLDPIRQTGGVRYLEN
HHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEECHHHHHCCHHHHCCCEEEECC
SDFLEDVYTMNDFIHSGGEKVLRTQSEVTGQVDKVPYLVTEYNGHMYPTKSFDQECKKVE
CHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHH
HAYRHLRVINESFGLDEISGAIGWCAFDYNTHSSFGSGDKICYHGVSDMFRNPKYAAYSY
HHHHHHHHHHHCCCCHHHCCCEEEEEEECCCCCCCCCCCCEEECCHHHHHCCCCCEEEEC
ASQKKVEDGVVLEPITLGAKGERDGGAILPFTVLTNCDYIKIFKDGIYIDTYYPNKEKFP
HHHHCCCCCEEEEEEEECCCCCCCCCEEEEEHHHCCCCEEEEEECCEEEEEECCCHHCCC
NLPHPPIEVSHILSMDSEIPLTEEAKKEIKDFVLNKLKDSNLTNLAEEDFKYIEEFSERV
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
NIPVFKIMSLVYKLAGGWGDKENSLIIKGFIDNKEVASKEIGELRSMNKLEVTPDDLELS
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEECCCCCCEEE
LDKISYDATRIVVKLLDNLGEVLFLNNDFIEVEIDGPLSIMGPSKFGISGGAVAFWVRTQ
HHHHCHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCEEEECCCCCCCCCCEEEEEEEEC
GKKGLCKIKVKSMYFEEEISIEVK
CCCCEEEEEEEEEEEEEEEEEEEC
>Mature Secondary Structure
MRKIIHINDQWVYANDYKSEYLKNEFDFSNFERVDLPHTNIELPYNYFDEKAYQFVSTYV
CCEEEEECCCEEEECCHHHHHHCCCCCCCCCEEECCCCCCEECCHHHHHHHHHHHHHHHH
KTLKFDENIKGKKVFLDFEGVMIAAEVYLNGIHVGGHKGGYTNFSIDITDALKINEDNIL
HHHCCCCCCCCCEEEEEECCEEEEEEEEEEEEEECCCCCCCEEEEEEEEEEEEECCCCEE
KVVVDSTERPDIPPHGYVVDYLTYGGIYREVSLRVVEPIFINNLYARAYDCLKEEKRLEL
EEEECCCCCCCCCCCCEEEEEHHHCCEEEEEEEEEECHHHHHHHHHHHHHHHHHCCCEEE
DIEINNFEKYRDDLEIVVEFGDDTFEETLSTKLPIEEGTSIKNIEIDQLNMVKLWDIENP
EEEECCHHHHCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEECCCC
KLYEIKVKLLKGSEVIDEYKDTFGFREAEFRSDGFYLNGRRVKLVGLNRHQAYPYVGYAM
EEEEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCEECCCC
PQRVQEKDAEILKYELGLNIVRTSHYPQSIYFLRKCDEIGLLVFEEIPGWQHIGDEAWQA
HHHHHHHHHHHEEHCCCCEEEEECCCCCHHHHHHHCCCCCEEEEECCCCHHHCCCHHHHH
ESIKNVEEMIKRDYNRPSIVLWGVRINESQDSHDFYVKTNAMAKSLDPIRQTGGVRYLEN
HHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEECHHHHHCCHHHHCCCEEEECC
SDFLEDVYTMNDFIHSGGEKVLRTQSEVTGQVDKVPYLVTEYNGHMYPTKSFDQECKKVE
CHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHH
HAYRHLRVINESFGLDEISGAIGWCAFDYNTHSSFGSGDKICYHGVSDMFRNPKYAAYSY
HHHHHHHHHHHCCCCHHHCCCEEEEEEECCCCCCCCCCCCEEECCHHHHHCCCCCEEEEC
ASQKKVEDGVVLEPITLGAKGERDGGAILPFTVLTNCDYIKIFKDGIYIDTYYPNKEKFP
HHHHCCCCCEEEEEEEECCCCCCCCCEEEEEHHHCCCCEEEEEECCEEEEEECCCHHCCC
NLPHPPIEVSHILSMDSEIPLTEEAKKEIKDFVLNKLKDSNLTNLAEEDFKYIEEFSERV
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
NIPVFKIMSLVYKLAGGWGDKENSLIIKGFIDNKEVASKEIGELRSMNKLEVTPDDLELS
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEECCCCCCEEE
LDKISYDATRIVVKLLDNLGEVLFLNNDFIEVEIDGPLSIMGPSKFGISGGAVAFWVRTQ
HHHHCHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCEEEECCCCCCCCCCEEEEEEEEC
GKKGLCKIKVKSMYFEEEISIEVK
CCCCEEEEEEEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840542 [H]