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The map label for this gene is ddl

Identifier: 18309801

GI number: 18309801

Start: 1003890

End: 1004798

Strand: Direct

Name: ddl

Synonym: CPE0819

Alternate gene names: 18309801

Gene position: 1003890-1004798 (Clockwise)

Preceding gene: 18309800

Following gene: 18309802

Centisome position: 33.12

GC content: 28.16

Gene sequence:

>909_bases
ATGAAGGTTGGAATAATAATGGGAGGAATTTCTTCAGAGAGAGAAGTTTCTTTAGCAAGTGGAGAATCAATTTTTAATCA
TATAGATAAAGAAAAGTATGAAGTTAGTAAAATAATTATAAATAATAAAATAGATATCTTAGAAAAAGTTAAAGGGTTAG
ATTTTGCTTTATTAGCACTACATGGTAAGTTTGGGGAGGATGGAACAGTTCAAGCTTTCTTAGATATAATGGATATACCA
TATTCAGGATGTGGAGCTTTAAGTAGTTCTTTATGTATGGATAAAAATCTAAGTAAGAAGGTTTTAAAAGCTGAAAACAT
AAGAACAGCAAAATGGATAACTGTGAAGTCTATAGAGGAAATAGATTATGAAAAGATTGAGGAAATTGGCTATCCAGTTT
TTGTTAAACCTAACAATGGGGGATCAAGTGTAGCTACCTTTAAGGTTTATAAAAAAGAAGATATAAAAAATTCTGTTATG
GAAGGATTAAAATATGATGAAGAAGTAATAATTGAATCCTTTATAAAAGGAAGAGAAATTACTTGTCCTATATTTAATGG
AGAGCTTTTCCCTATTCTTGAAATAAAGAGCAAAGCAGATTTTTATGATTATAAGCAAAAGTATGCAGCTAATGGAGCAG
AACATTTACCAGTTCAGTTAGAAAAATCTTTATATGATGAAGTTAAGGAAATGGCTTTAAAAACTTTTGAAGTATTAAAA
TGTGAAGTTTATGCTAGAGTGGATATGATAATTTCAGAAGAAGGAGTTCCATATATACTAGAAGTAAATACATTACCTGG
TATGACAGCTACAAGTTTATTTCCACAAAGTGCAGAATCTAGAGGAATATCATATTCAAAATTTATAGATTTAATAATAG
AAACATCATTAAATAAGAAAATAAAATAA

Upstream 100 bases:

>100_bases
AGCTTATGTGGTTCTTACCTTTATATGCAAAAATTCTATAATAATATTATAGAGCTAAATTTGGATTTACTTATAATAAT
TATAGTTGGAGGACAATAAT

Downstream 100 bases:

>100_bases
ATAGAAGTTTTCAAAAGTCATTTATTTAAAGATAATTATTTTAATAAATGGCTTTTCATATACTCCTTTGAATAATATTT
AGATATATTGTATATTAGAT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase B; D-alanylalanine synthetaseB

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKVGIIMGGISSEREVSLASGESIFNHIDKEKYEVSKIIINNKIDILEKVKGLDFALLALHGKFGEDGTVQAFLDIMDIP
YSGCGALSSSLCMDKNLSKKVLKAENIRTAKWITVKSIEEIDYEKIEEIGYPVFVKPNNGGSSVATFKVYKKEDIKNSVM
EGLKYDEEVIIESFIKGREITCPIFNGELFPILEIKSKADFYDYKQKYAANGAEHLPVQLEKSLYDEVKEMALKTFEVLK
CEVYARVDMIISEEGVPYILEVNTLPGMTATSLFPQSAESRGISYSKFIDLIIETSLNKKIK

Sequences:

>Translated_302_residues
MKVGIIMGGISSEREVSLASGESIFNHIDKEKYEVSKIIINNKIDILEKVKGLDFALLALHGKFGEDGTVQAFLDIMDIP
YSGCGALSSSLCMDKNLSKKVLKAENIRTAKWITVKSIEEIDYEKIEEIGYPVFVKPNNGGSSVATFKVYKKEDIKNSVM
EGLKYDEEVIIESFIKGREITCPIFNGELFPILEIKSKADFYDYKQKYAANGAEHLPVQLEKSLYDEVKEMALKTFEVLK
CEVYARVDMIISEEGVPYILEVNTLPGMTATSLFPQSAESRGISYSKFIDLIIETSLNKKIK
>Mature_302_residues
MKVGIIMGGISSEREVSLASGESIFNHIDKEKYEVSKIIINNKIDILEKVKGLDFALLALHGKFGEDGTVQAFLDIMDIP
YSGCGALSSSLCMDKNLSKKVLKAENIRTAKWITVKSIEEIDYEKIEEIGYPVFVKPNNGGSSVATFKVYKKEDIKNSVM
EGLKYDEEVIIESFIKGREITCPIFNGELFPILEIKSKADFYDYKQKYAANGAEHLPVQLEKSLYDEVKEMALKTFEVLK
CEVYARVDMIISEEGVPYILEVNTLPGMTATSLFPQSAESRGISYSKFIDLIIETSLNKKIK

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=304, Percent_Identity=34.2105263157895, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1786579, Length=350, Percent_Identity=30.8571428571429, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDLB_CLOPE (Q8XM71)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_561735.1
- ProteinModelPortal:   Q8XM71
- SMR:   Q8XM71
- GeneID:   989078
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE0819
- NMPDR:   fig|195102.1.peg.882
- HOGENOM:   HBG427092
- OMA:   KYMDFSR
- PhylomeDB:   Q8XM71
- ProtClustDB:   PRK01372
- BioCyc:   CPER195102:CPE0819-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33878; Mature: 33878

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGIIMGGISSEREVSLASGESIFNHIDKEKYEVSKIIINNKIDILEKVKGLDFALLAL
CEEEEEECCCCCCCEEEHHCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCEEEEEE
HGKFGEDGTVQAFLDIMDIPYSGCGALSSSLCMDKNLSKKVLKAENIRTAKWITVKSIEE
ECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEHHHHH
IDYEKIEEIGYPVFVKPNNGGSSVATFKVYKKEDIKNSVMEGLKYDEEVIIESFIKGREI
CCHHHHHHCCCCEEEEECCCCCCEEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHCCCEE
TCPIFNGELFPILEIKSKADFYDYKQKYAANGAEHLPVQLEKSLYDEVKEMALKTFEVLK
EEEEECCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CEVYARVDMIISEEGVPYILEVNTLPGMTATSLFPQSAESRGISYSKFIDLIIETSLNKK
HHHHHHEEEEECCCCCCEEEEECCCCCCCHHHCCCCCHHHCCCCHHHHHHHHHHHCCCCC
IK
CC
>Mature Secondary Structure
MKVGIIMGGISSEREVSLASGESIFNHIDKEKYEVSKIIINNKIDILEKVKGLDFALLAL
CEEEEEECCCCCCCEEEHHCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCEEEEEE
HGKFGEDGTVQAFLDIMDIPYSGCGALSSSLCMDKNLSKKVLKAENIRTAKWITVKSIEE
ECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEHHHHH
IDYEKIEEIGYPVFVKPNNGGSSVATFKVYKKEDIKNSVMEGLKYDEEVIIESFIKGREI
CCHHHHHHCCCCEEEEECCCCCCEEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHCCCEE
TCPIFNGELFPILEIKSKADFYDYKQKYAANGAEHLPVQLEKSLYDEVKEMALKTFEVLK
EEEEECCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CEVYARVDMIISEEGVPYILEVNTLPGMTATSLFPQSAESRGISYSKFIDLIIETSLNKK
HHHHHHEEEEECCCCCCEEEEECCCCCCCHHHCCCCCHHHCCCCHHHHHHHHHHHCCCCC
IK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842