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The map label for this gene is lipB [H]

Identifier: 18309724

GI number: 18309724

Start: 918827

End: 920548

Strand: Direct

Name: lipB [H]

Synonym: CPE0742

Alternate gene names: 18309724

Gene position: 918827-920548 (Clockwise)

Preceding gene: 18309723

Following gene: 18309725

Centisome position: 30.31

GC content: 26.77

Gene sequence:

>1722_bases
ATGGACAAAAATTTAAAAGATTTTAATGGTATTAAAGGAACAGAGGACAACTTAACTGGAATAGCTAAAGCAAATTTTAA
TACTGAACATGGAATTAGAAATTTAGTACTTTGGGGAAAAGAAGTAGATGAAAATTCTTATTTATCTCTAGGAATTTTAA
AAAGATTTCATAAATATTATGGCACTGATAATTCAGAAATAAAATTTGAAAAAGTATTATCTGATAGATTTGATGAAGAG
GTTTTCAGTAAGAATAATGCTAATTTAATTTTGCTTGTAAATAGTATTAATGACTTAATTAGGTTATGTAGTGATAAATC
AAAGGAAAATGAGGAAAATTTAAATCTAATTATAAAAAGATTTGTGAGATTAATAGAGATTGCTCATAAAAATAGGGCGA
GAATTATATTTACAACAATACCTCCATTTAGTGGTGAAAATAAAAATTTAGAGTATGTAAGAAATGAGATAAACTCTTGG
ATTAGAAAGAGTACATTTTTAGATGGATATTTAGATTTAGACAAAATTGTTGAAAAGAGACTAGGTGTTTCTAAAGATAA
GAAAGAGATTAATTATGATAAAGAATTAGAAGAATATATGGCAGAAAATATTTCTTTAGATTATATTGTAGAAAGACTTA
AACCTTTTGAGCTTGACCACATGAGCCAAAGTGATTTAATAAAAGCTATGAATGAGAATTCAAGGTTTATTAATGAAGAT
GGTGTTGATATTTTAGTTAAGCCAATACCAGATCTAGTTAAAGGGACAAGAATAGATAGAAGAATTAAATATTTTGATGA
GTATAAAAGACCTAAAAGATCAGGAAATCCTTATGTATTTAATGGGGAAGCAGTTGGAGATATGAGAGATAATATGGGAT
TATTAAATTTAAATCTCTGCAAAAGTAATATATTGATGAGTAAAGAAAATATAAATGGAGTTAATTGCAGAGTTTATAAG
AAAGAAGGATTAAAAGAAAATTTACCATGTATAGTTTATATACATGGAGGTGCATTTATTGGAGGAAGCTTAGATGTTTC
TGAAAATCCTTGTAAACTTATAGCTGAGGGTATTAATGGAGTTGTAATAAGCGTTGATTATTCCTTAGCACCAGAAAAGC
CATATCCTCTAGGATTAAATGATTGTAGAAAAGTTGTTGAATATATTGAAGAAAATAATTTTTTATATGGAATAGATAAA
AGTAAAATAGGAATAGTAGGGGAAAGTGCAGGAGCTAATTTAGCAACAATTGTAGCTAATGAGAATTCAAATATAAAGTT
TCAAGGGCTTGTATATCCTGTAGTTACTTTTGTAGAGAAAAATCCATTTTTTAATTGGGATATAGATTTATATAAGAATC
CATATAAGGAAGAAAAAATATATAATTTTATTAATTCTTTGAGAAATTGTGAGGATTTAGTACAAAAACTTTACATACAA
AGAACGTTAGATCCTAGAAGAGAAGATTTATCACCAATATTTAATAAAAATCTTTCAAAGGCTAAAAAAACATTAATTGC
AGTTTCTGAATATGATTATTTAAGAGTACAGGGAGAAGCTTATGGTAAGCTTATTCATAAAGCGGGAGTAGAAACAAAAA
TAATAAGATATGAAGGGGTAAACCATGCGTTTTTAGATAACCTTGGAATATACCCTCAAGCAGAAGATACAATAAATGAA
ATTGTAAAAGAGTTTTTAGATGCTATAGGAAATAAATTTTAA

Upstream 100 bases:

>100_bases
AATCATCTTTAACTAAACGGGTATAATAAAATAGTTTTAAGGTGGTTAAATAATTTAAAAGGTTATTAGTTTTATTTATA
AAGAGAGGATATGAGGTTAG

Downstream 100 bases:

>100_bases
TTGAAGTTATAAAAATAATGTGTGTATTAAAAGGCTATAAGAAAACTTATAGTGGTGTGTGGATTATTAAAGACTATTAA
TAAGTTTTGTTTGCTAAGCT

Product: lipase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MDKNLKDFNGIKGTEDNLTGIAKANFNTEHGIRNLVLWGKEVDENSYLSLGILKRFHKYYGTDNSEIKFEKVLSDRFDEE
VFSKNNANLILLVNSINDLIRLCSDKSKENEENLNLIIKRFVRLIEIAHKNRARIIFTTIPPFSGENKNLEYVRNEINSW
IRKSTFLDGYLDLDKIVEKRLGVSKDKKEINYDKELEEYMAENISLDYIVERLKPFELDHMSQSDLIKAMNENSRFINED
GVDILVKPIPDLVKGTRIDRRIKYFDEYKRPKRSGNPYVFNGEAVGDMRDNMGLLNLNLCKSNILMSKENINGVNCRVYK
KEGLKENLPCIVYIHGGAFIGGSLDVSENPCKLIAEGINGVVISVDYSLAPEKPYPLGLNDCRKVVEYIEENNFLYGIDK
SKIGIVGESAGANLATIVANENSNIKFQGLVYPVVTFVEKNPFFNWDIDLYKNPYKEEKIYNFINSLRNCEDLVQKLYIQ
RTLDPRREDLSPIFNKNLSKAKKTLIAVSEYDYLRVQGEAYGKLIHKAGVETKIIRYEGVNHAFLDNLGIYPQAEDTINE
IVKEFLDAIGNKF

Sequences:

>Translated_573_residues
MDKNLKDFNGIKGTEDNLTGIAKANFNTEHGIRNLVLWGKEVDENSYLSLGILKRFHKYYGTDNSEIKFEKVLSDRFDEE
VFSKNNANLILLVNSINDLIRLCSDKSKENEENLNLIIKRFVRLIEIAHKNRARIIFTTIPPFSGENKNLEYVRNEINSW
IRKSTFLDGYLDLDKIVEKRLGVSKDKKEINYDKELEEYMAENISLDYIVERLKPFELDHMSQSDLIKAMNENSRFINED
GVDILVKPIPDLVKGTRIDRRIKYFDEYKRPKRSGNPYVFNGEAVGDMRDNMGLLNLNLCKSNILMSKENINGVNCRVYK
KEGLKENLPCIVYIHGGAFIGGSLDVSENPCKLIAEGINGVVISVDYSLAPEKPYPLGLNDCRKVVEYIEENNFLYGIDK
SKIGIVGESAGANLATIVANENSNIKFQGLVYPVVTFVEKNPFFNWDIDLYKNPYKEEKIYNFINSLRNCEDLVQKLYIQ
RTLDPRREDLSPIFNKNLSKAKKTLIAVSEYDYLRVQGEAYGKLIHKAGVETKIIRYEGVNHAFLDNLGIYPQAEDTINE
IVKEFLDAIGNKF
>Mature_573_residues
MDKNLKDFNGIKGTEDNLTGIAKANFNTEHGIRNLVLWGKEVDENSYLSLGILKRFHKYYGTDNSEIKFEKVLSDRFDEE
VFSKNNANLILLVNSINDLIRLCSDKSKENEENLNLIIKRFVRLIEIAHKNRARIIFTTIPPFSGENKNLEYVRNEINSW
IRKSTFLDGYLDLDKIVEKRLGVSKDKKEINYDKELEEYMAENISLDYIVERLKPFELDHMSQSDLIKAMNENSRFINED
GVDILVKPIPDLVKGTRIDRRIKYFDEYKRPKRSGNPYVFNGEAVGDMRDNMGLLNLNLCKSNILMSKENINGVNCRVYK
KEGLKENLPCIVYIHGGAFIGGSLDVSENPCKLIAEGINGVVISVDYSLAPEKPYPLGLNDCRKVVEYIEENNFLYGIDK
SKIGIVGESAGANLATIVANENSNIKFQGLVYPVVTFVEKNPFFNWDIDLYKNPYKEEKIYNFINSLRNCEDLVQKLYIQ
RTLDPRREDLSPIFNKNLSKAKKTLIAVSEYDYLRVQGEAYGKLIHKAGVETKIIRYEGVNHAFLDNLGIYPQAEDTINE
IVKEFLDAIGNKF

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68051721, Length=308, Percent_Identity=23.7012987012987, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI68299767, Length=148, Percent_Identity=31.0810810810811, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI226423947, Length=317, Percent_Identity=23.6593059936909, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1786682, Length=89, Percent_Identity=37.0786516853933, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540028, Length=400, Percent_Identity=25.5, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71996133, Length=397, Percent_Identity=24.9370277078086, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI72001146, Length=220, Percent_Identity=30.4545454545455, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17567059, Length=328, Percent_Identity=28.0487804878049, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 65798; Mature: 65798

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKNLKDFNGIKGTEDNLTGIAKANFNTEHGIRNLVLWGKEVDENSYLSLGILKRFHKYY
CCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
GTDNSEIKFEKVLSDRFDEEVFSKNNANLILLVNSINDLIRLCSDKSKENEENLNLIIKR
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHH
FVRLIEIAHKNRARIIFTTIPPFSGENKNLEYVRNEINSWIRKSTFLDGYLDLDKIVEKR
HHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LGVSKDKKEINYDKELEEYMAENISLDYIVERLKPFELDHMSQSDLIKAMNENSRFINED
CCCCCCHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEECCC
GVDILVKPIPDLVKGTRIDRRIKYFDEYKRPKRSGNPYVFNGEAVGDMRDNMGLLNLNLC
CCEEEEECCHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHCCCEEEEEEH
KSNILMSKENINGVNCRVYKKEGLKENLPCIVYIHGGAFIGGSLDVSENPCKLIAEGING
HCHHEEECCCCCCCEEEEEECCCCCCCCCEEEEEECCEEECCCCCCCCCHHHHHHCCCCE
VVISVDYSLAPEKPYPLGLNDCRKVVEYIEENNFLYGIDKSKIGIVGESAGANLATIVAN
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCEEEEEEC
ENSNIKFQGLVYPVVTFVEKNPFFNWDIDLYKNPYKEEKIYNFINSLRNCEDLVQKLYIQ
CCCCEEEEEEHHHHHHHHHCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RTLDPRREDLSPIFNKNLSKAKKTLIAVSEYDYLRVQGEAYGKLIHKAGVETKIIRYEGV
HCCCCCHHHCCHHHCCCHHHHHHHEEEEECCCEEEECCHHHHHHHHHCCCCEEEEEECCC
NHAFLDNLGIYPQAEDTINEIVKEFLDAIGNKF
CHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDKNLKDFNGIKGTEDNLTGIAKANFNTEHGIRNLVLWGKEVDENSYLSLGILKRFHKYY
CCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
GTDNSEIKFEKVLSDRFDEEVFSKNNANLILLVNSINDLIRLCSDKSKENEENLNLIIKR
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHH
FVRLIEIAHKNRARIIFTTIPPFSGENKNLEYVRNEINSWIRKSTFLDGYLDLDKIVEKR
HHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LGVSKDKKEINYDKELEEYMAENISLDYIVERLKPFELDHMSQSDLIKAMNENSRFINED
CCCCCCHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEECCC
GVDILVKPIPDLVKGTRIDRRIKYFDEYKRPKRSGNPYVFNGEAVGDMRDNMGLLNLNLC
CCEEEEECCHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHCCCEEEEEEH
KSNILMSKENINGVNCRVYKKEGLKENLPCIVYIHGGAFIGGSLDVSENPCKLIAEGING
HCHHEEECCCCCCCEEEEEECCCCCCCCCEEEEEECCEEECCCCCCCCCHHHHHHCCCCE
VVISVDYSLAPEKPYPLGLNDCRKVVEYIEENNFLYGIDKSKIGIVGESAGANLATIVAN
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCEEEEEEC
ENSNIKFQGLVYPVVTFVEKNPFFNWDIDLYKNPYKEEKIYNFINSLRNCEDLVQKLYIQ
CCCCEEEEEEHHHHHHHHHCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RTLDPRREDLSPIFNKNLSKAKKTLIAVSEYDYLRVQGEAYGKLIHKAGVETKIIRYEGV
HCCCCCHHHCCHHHCCCHHHHHHHEEEEECCCEEEECCHHHHHHHHHCCCCEEEEEECCC
NHAFLDNLGIYPQAEDTINEIVKEFLDAIGNKF
CHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA