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The map label for this gene is supH [H]

Identifier: 18309705

GI number: 18309705

Start: 898316

End: 899104

Strand: Direct

Name: supH [H]

Synonym: CPE0723

Alternate gene names: 18309705

Gene position: 898316-899104 (Clockwise)

Preceding gene: 18309704

Following gene: 18309706

Centisome position: 29.63

GC content: 28.64

Gene sequence:

>789_bases
ATGATAAAATTTATAGCAACAGATATGGATGGAACTTTATTAAATAGTAAGAAAGAGTTAAGTCCAGAATTCTATGATGT
ATTTGAGGAACTAAAGAAAAGAAAGATACTTTTTGCAGCTGCAAGTGGAAGACAATATTATACTTTAGCTAAAGAGTTTA
ATGATATAAAAGATGACATGCTATTTATTGCTGAAAATGGAACTTTCGTGGTTTATAAAGGAAAAGAGCTTGTTGTTAAT
GGATTAGATAGGGAATTAGCTAATGAACTTATAAGAATAGGAAGAACTATAGAAAATTCAAATGTAGTTTTATGTGGAAA
GAATTCAGCATATGTTGAAAGTAGTGATGAAAGATTCTTAGGAGAAGTTAGAAAATACTATGAAAGATGCGAAATAGTAG
ATGATTTAGAAAAGGTAGATGATACGGTTTTAAAAGTTACTATGTGCGATTTTAATGGTTCAGAAGAAAATAGCAATAAA
TATTTTGATAAGTATAGAGAGGAACTTCAAGTAACTGTTTCAGGGGATATATGGTTAGATATAACTGCTGGAGGAGTTAA
TAAAGGAGTGGCCATAAAAGAAATTCAAGATCTTTTAGGAATAGACTTTAAAGAAACTATGGTATTTGGAGATTATTTAA
ATGATTTAGAAATGATGGAAAGTGCTTATCATAGTTATGCAATGGAAAATGCTCATGAAGATTTAAAGAAGGTTTCAAGA
TTTATAACTCATAAAAATAATGATGAAGATGGAGTTATGCATCAAATAAAAGAAGTTATAGGATTATAG

Upstream 100 bases:

>100_bases
TTGATTATTTTAAATAAATTATTTTAGAATTTATATTAAATATATAGAATTAAACATAAAATTAACTTTAATTTATACTT
TTACTTAGGAGGATATATAT

Downstream 100 bases:

>100_bases
AATTAATTAGAGAGTTGTATTAAAGCAACTCTTTTTTATTTTAATACCATTAATAGTATTTACTCTATAGTTGAAATTTT
CTATATGTCATCTAAAACAT

Product: HAD hydrolase, IIB family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MIKFIATDMDGTLLNSKKELSPEFYDVFEELKKRKILFAAASGRQYYTLAKEFNDIKDDMLFIAENGTFVVYKGKELVVN
GLDRELANELIRIGRTIENSNVVLCGKNSAYVESSDERFLGEVRKYYERCEIVDDLEKVDDTVLKVTMCDFNGSEENSNK
YFDKYREELQVTVSGDIWLDITAGGVNKGVAIKEIQDLLGIDFKETMVFGDYLNDLEMMESAYHSYAMENAHEDLKKVSR
FITHKNNDEDGVMHQIKEVIGL

Sequences:

>Translated_262_residues
MIKFIATDMDGTLLNSKKELSPEFYDVFEELKKRKILFAAASGRQYYTLAKEFNDIKDDMLFIAENGTFVVYKGKELVVN
GLDRELANELIRIGRTIENSNVVLCGKNSAYVESSDERFLGEVRKYYERCEIVDDLEKVDDTVLKVTMCDFNGSEENSNK
YFDKYREELQVTVSGDIWLDITAGGVNKGVAIKEIQDLLGIDFKETMVFGDYLNDLEMMESAYHSYAMENAHEDLKKVSR
FITHKNNDEDGVMHQIKEVIGL
>Mature_262_residues
MIKFIATDMDGTLLNSKKELSPEFYDVFEELKKRKILFAAASGRQYYTLAKEFNDIKDDMLFIAENGTFVVYKGKELVVN
GLDRELANELIRIGRTIENSNVVLCGKNSAYVESSDERFLGEVRKYYERCEIVDDLEKVDDTVLKVTMCDFNGSEENSNK
YFDKYREELQVTVSGDIWLDITAGGVNKGVAIKEIQDLLGIDFKETMVFGDYLNDLEMMESAYHSYAMENAHEDLKKVSR
FITHKNNDEDGVMHQIKEVIGL

Specific function: Catalyzes the hydrolysis of sugar phosphate to sugar and inorganic phosphate. Has a wide substrate specificity catalyzing the hydrolysis of fructose-1-P most efficiently, but it remains uncertain if this is the real substrate in vivo [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family. SupH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787043, Length=267, Percent_Identity=35.9550561797753, Blast_Score=147, Evalue=5e-37,
Organism=Escherichia coli, GI87081790, Length=265, Percent_Identity=34.3396226415094, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI2367265, Length=274, Percent_Identity=29.1970802919708, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI87081741, Length=267, Percent_Identity=24.7191011235955, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: =3.1.3.23 [H]

Molecular weight: Translated: 30068; Mature: 30068

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFIATDMDGTLLNSKKELSPEFYDVFEELKKRKILFAAASGRQYYTLAKEFNDIKDDM
CEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHCEEEEEECCCCEEEHHHHHHCCCCCCE
LFIAENGTFVVYKGKELVVNGLDRELANELIRIGRTIENSNVVLCGKNSAYVESSDERFL
EEEECCCEEEEEECCEEEECCCCHHHHHHHHHHCCEECCCCEEEECCCCCEECCCHHHHH
GEVRKYYERCEIVDDLEKVDDTVLKVTMCDFNGSEENSNKYFDKYREELQVTVSGDIWLD
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHEEEEEECCEEEE
ITAGGVNKGVAIKEIQDLLGIDFKETMVFGDYLNDLEMMESAYHSYAMENAHEDLKKVSR
EEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FITHKNNDEDGVMHQIKEVIGL
HHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIKFIATDMDGTLLNSKKELSPEFYDVFEELKKRKILFAAASGRQYYTLAKEFNDIKDDM
CEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHCEEEEEECCCCEEEHHHHHHCCCCCCE
LFIAENGTFVVYKGKELVVNGLDRELANELIRIGRTIENSNVVLCGKNSAYVESSDERFL
EEEECCCEEEEEECCEEEECCCCHHHHHHHHHHCCEECCCCEEEECCCCCEECCCHHHHH
GEVRKYYERCEIVDDLEKVDDTVLKVTMCDFNGSEENSNKYFDKYREELQVTVSGDIWLD
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHEEEEEECCEEEE
ITAGGVNKGVAIKEIQDLLGIDFKETMVFGDYLNDLEMMESAYHSYAMENAHEDLKKVSR
EEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FITHKNNDEDGVMHQIKEVIGL
HHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]