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The map label for this gene is alaXL [H]

Identifier: 18309700

GI number: 18309700

Start: 892351

End: 893553

Strand: Direct

Name: alaXL [H]

Synonym: CPE0718

Alternate gene names: 18309700

Gene position: 892351-893553 (Clockwise)

Preceding gene: 18309699

Following gene: 18309701

Centisome position: 29.44

GC content: 28.43

Gene sequence:

>1203_bases
ATGACAAAACTTTATTATGAAGATCAATATATAAAGGAGTTTAAAGGAGAAATAATTGAGGTTAAAGAAATTGATGGAAA
ATTCCATGTTCTTTTAGACCAAACTGCCTTTTTCCCAGGAGGGGGAGGACAGATGGGAGATTTAGGACTTATTGATGGAA
TTAAAGTCCTTGATGTTTATGAAGAAGAAGGAAAAGTATATCATGTTCTTGAAAAAGAACCTAAAAAATTGAAAAATCTA
CAATGTGAATTAGATTGGGAAAGAAGATTTGATGGAATGCAACAGCATTTAGGACAGCATTTACTTTCTGGATGTTTTTA
TGATTTATTTGGTGCAAATACTTGTGGTTTTCATTTAGGAAAAGAAATAAGTACAGTTGATATTGTAGGTTTTTTAGATG
AAAAAACTATAAGAGAAGCTGAAAAAGAGGCAAATAGATTAATCTTTGAGAACTTAGAAGTAAAAAGTTATGCTCCAAGC
AAAAAAGAATTAAAGAAAGTAAAAACTAGAAGAGCATTGCCTAAAACTGATGAAGAAATAAGAATTGTTGAAATTGTAGG
ATTAGATTTAAATGCTTGTTGTGGAGTTCATCCAAGAAATACTAGAGATCTTCAAGTTATAAAGATAAGAAGATGGGAAA
AACATAAAAATGCTACAAGAATAGAATATGTAGCTGGAAATAGAGCAGTATCTGACTTTTTCACTAAGGATGAAATTTTA
GGAGAAATATGCAAGCTTTTAAAATCAGGAGAAGGTGATACTTTAAACGCTGTTAAGAATCTTTTAGAAAATAATAAAAA
TCTTGTAGATGAAAACAGAAAAGTTAAAGCTGAAATAGGGGATTATAAGATAAAAGAGATGTTAAATAAATCAGAAAGAA
TAGGAAGTATAACTTTAGTTAATGAAATTTTTGATGGTGAGGATACAAAACATATAGGAAAGTTAGCTAATAAAATAACT
GAGGAATATGAAGCAATAGTTTTATTTGCAGTTAAAAATGGAGATAGAGTAAATTTAATTTTCAATAGTTCTAAAGATAT
GAAAAAAGTTAATATGAGTGATATATTAAAAGATACCATAACTTTAATTGATGGAAGAGGTGGTGGAAATCAGTTTGCAG
CTCAAGGTGGAGGAAAAAATAATGGAAATACAGAAGTTGCAATTGATTATGCTACTAATAAAATAAGAAATATTTTACTT
TAA

Upstream 100 bases:

>100_bases
ATTTAATAAATAAATGATATAATTTATTTTAGAGCTAATTTTTATAAAAGTTAAGTTTAAGCTCTTATTATTAAGAAGAC
AATTGAGGTGTTGATATAAA

Downstream 100 bases:

>100_bases
ATTTTAAAACTATTTATAAGCTTAATTTAAAAACTATAATGGAGTTGAAGTGAGATGGCATTAATGAGTGATAAGTTTTT
AAGTATAGTAATTCCTGCAT

Product: DHHA1 domain protein

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTKLYYEDQYIKEFKGEIIEVKEIDGKFHVLLDQTAFFPGGGGQMGDLGLIDGIKVLDVYEEEGKVYHVLEKEPKKLKNL
QCELDWERRFDGMQQHLGQHLLSGCFYDLFGANTCGFHLGKEISTVDIVGFLDEKTIREAEKEANRLIFENLEVKSYAPS
KKELKKVKTRRALPKTDEEIRIVEIVGLDLNACCGVHPRNTRDLQVIKIRRWEKHKNATRIEYVAGNRAVSDFFTKDEIL
GEICKLLKSGEGDTLNAVKNLLENNKNLVDENRKVKAEIGDYKIKEMLNKSERIGSITLVNEIFDGEDTKHIGKLANKIT
EEYEAIVLFAVKNGDRVNLIFNSSKDMKKVNMSDILKDTITLIDGRGGGNQFAAQGGGKNNGNTEVAIDYATNKIRNILL

Sequences:

>Translated_400_residues
MTKLYYEDQYIKEFKGEIIEVKEIDGKFHVLLDQTAFFPGGGGQMGDLGLIDGIKVLDVYEEEGKVYHVLEKEPKKLKNL
QCELDWERRFDGMQQHLGQHLLSGCFYDLFGANTCGFHLGKEISTVDIVGFLDEKTIREAEKEANRLIFENLEVKSYAPS
KKELKKVKTRRALPKTDEEIRIVEIVGLDLNACCGVHPRNTRDLQVIKIRRWEKHKNATRIEYVAGNRAVSDFFTKDEIL
GEICKLLKSGEGDTLNAVKNLLENNKNLVDENRKVKAEIGDYKIKEMLNKSERIGSITLVNEIFDGEDTKHIGKLANKIT
EEYEAIVLFAVKNGDRVNLIFNSSKDMKKVNMSDILKDTITLIDGRGGGNQFAAQGGGKNNGNTEVAIDYATNKIRNILL
>Mature_399_residues
TKLYYEDQYIKEFKGEIIEVKEIDGKFHVLLDQTAFFPGGGGQMGDLGLIDGIKVLDVYEEEGKVYHVLEKEPKKLKNLQ
CELDWERRFDGMQQHLGQHLLSGCFYDLFGANTCGFHLGKEISTVDIVGFLDEKTIREAEKEANRLIFENLEVKSYAPSK
KELKKVKTRRALPKTDEEIRIVEIVGLDLNACCGVHPRNTRDLQVIKIRRWEKHKNATRIEYVAGNRAVSDFFTKDEILG
EICKLLKSGEGDTLNAVKNLLENNKNLVDENRKVKAEIGDYKIKEMLNKSERIGSITLVNEIFDGEDTKHIGKLANKITE
EYEAIVLFAVKNGDRVNLIFNSSKDMKKVNMSDILKDTITLIDGRGGGNQFAAQGGGKNNGNTEVAIDYATNKIRNILL

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=315, Percent_Identity=32.0634920634921, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI217416402, Length=315, Percent_Identity=32.0634920634921, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1789048, Length=375, Percent_Identity=24.2666666666667, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI18543305, Length=286, Percent_Identity=31.1188811188811, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 45293; Mature: 45162

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLYYEDQYIKEFKGEIIEVKEIDGKFHVLLDQTAFFPGGGGQMGDLGLIDGIKVLDVY
CCCEEECHHHHHHHCCCEEEEEECCCEEEEEEECCEECCCCCCCCCCCCHHCCEEEEEEE
EEEGKVYHVLEKEPKKLKNLQCELDWERRFDGMQQHLGQHLLSGCFYDLFGANTCGFHLG
CCCCCEEEEECCCCHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCC
KEISTVDIVGFLDEKTIREAEKEANRLIFENLEVKSYAPSKKELKKVKTRRALPKTDEEI
CCCCEEEEEEHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCE
RIVEIVGLDLNACCGVHPRNTRDLQVIKIRRWEKHKNATRIEYVAGNRAVSDFFTKDEIL
EEEEEECCCHHHHCCCCCCCCCCEEEEEEEHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
GEICKLLKSGEGDTLNAVKNLLENNKNLVDENRKVKAEIGDYKIKEMLNKSERIGSITLV
HHHHHHHHCCCCCHHHHHHHHHHCCCCHHCCCCEEEEHHCCHHHHHHHCHHHHCCEEEEE
NEIFDGEDTKHIGKLANKITEEYEAIVLFAVKNGDRVNLIFNSSKDMKKVNMSDILKDTI
HHHHCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCCHHHCCHHHHHHHHH
TLIDGRGGGNQFAAQGGGKNNGNTEVAIDYATNKIRNILL
HEEECCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHCC
>Mature Secondary Structure 
TKLYYEDQYIKEFKGEIIEVKEIDGKFHVLLDQTAFFPGGGGQMGDLGLIDGIKVLDVY
CCEEECHHHHHHHCCCEEEEEECCCEEEEEEECCEECCCCCCCCCCCCHHCCEEEEEEE
EEEGKVYHVLEKEPKKLKNLQCELDWERRFDGMQQHLGQHLLSGCFYDLFGANTCGFHLG
CCCCCEEEEECCCCHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCC
KEISTVDIVGFLDEKTIREAEKEANRLIFENLEVKSYAPSKKELKKVKTRRALPKTDEEI
CCCCEEEEEEHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCE
RIVEIVGLDLNACCGVHPRNTRDLQVIKIRRWEKHKNATRIEYVAGNRAVSDFFTKDEIL
EEEEEECCCHHHHCCCCCCCCCCEEEEEEEHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
GEICKLLKSGEGDTLNAVKNLLENNKNLVDENRKVKAEIGDYKIKEMLNKSERIGSITLV
HHHHHHHHCCCCCHHHHHHHHHHCCCCHHCCCCEEEEHHCCHHHHHHHCHHHHCCEEEEE
NEIFDGEDTKHIGKLANKITEEYEAIVLFAVKNGDRVNLIFNSSKDMKKVNMSDILKDTI
HHHHCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCCHHHCCHHHHHHHHH
TLIDGRGGGNQFAAQGGGKNNGNTEVAIDYATNKIRNILL
HEEECCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]