Click here to switch to the map view.

The map label for this gene is purN [H]

Identifier: 18309667

GI number: 18309667

Start: 853093

End: 853707

Strand: Direct

Name: purN [H]

Synonym: CPE0685

Alternate gene names: 18309667

Gene position: 853093-853707 (Clockwise)

Preceding gene: 18309666

Following gene: 18309668

Centisome position: 28.14

GC content: 30.08

Gene sequence:

>615_bases
TTGTATAAAATAGCTGTATTAGCTTCTGGCAGTGGATCTAACCTTCAATCAATTTTAGATAATATAAATAATGGGAATAT
AAAGGGAGAAGTTTCTTTGGTTATTGGAAGTAAGGAGGATATTTTTGCCTTAGAAAGGGCTGAAAAACAGGGTATAAAAA
CATCAGTTGTTTCTAAAAAAGAGTTTGGAGATAAAACTTCAGATGAAATCTTAAGACTTGCTAAGGAAAATAATATTCAT
TTAATTGTACTAGCAGGCTATCTTTCAATATTAAAAGGAAAACTTTTAGAAGAGTATGGAAATAGGATAATTAATATCCA
TCCATCTCTTATTCCTTCTTTCTGTGGAAATAAAATGTATGGAATAAATGTACATAAGGCAGCTATAGAAAAGGGAGTGA
AATTTTCTGGGTGTACAGTTCACTTTGTTAATGATGAGGTTGATGGAGGAGCTATTATTGCACAAGAAATAGTTGAAGTT
AATTTTGAAGATACTCCAGAAAGTCTTCAAAAAAAGGTTTTAGAGAAGGAGCATATATTGCTACCTAGAATTGTTAAGTA
CTTATGCGAAGAAAAAATAGAAATTCATAATGGAAAAGTGAAAATATTAAACTAG

Upstream 100 bases:

>100_bases
GTGTTGAGGAAGATGAGGTTGAAGAAGTTTTAAAAGAACTTTCAAAGCAAGGAGAAAAAGCTTTTGAGATTGGGTATATA
AATGCTGGAGGTGAAGGAGT

Downstream 100 bases:

>100_bases
GGAGGTCTTTTAATGAAAAAGAGAGCTTTAATAAGTGTTTTTGATAAAGATGGAGTTTTAGAACTTGCAAAATTTTTAAG
AGATAGAGATGTAGAAATAA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MYKIAVLASGSGSNLQSILDNINNGNIKGEVSLVIGSKEDIFALERAEKQGIKTSVVSKKEFGDKTSDEILRLAKENNIH
LIVLAGYLSILKGKLLEEYGNRIINIHPSLIPSFCGNKMYGINVHKAAIEKGVKFSGCTVHFVNDEVDGGAIIAQEIVEV
NFEDTPESLQKKVLEKEHILLPRIVKYLCEEKIEIHNGKVKILN

Sequences:

>Translated_204_residues
MYKIAVLASGSGSNLQSILDNINNGNIKGEVSLVIGSKEDIFALERAEKQGIKTSVVSKKEFGDKTSDEILRLAKENNIH
LIVLAGYLSILKGKLLEEYGNRIINIHPSLIPSFCGNKMYGINVHKAAIEKGVKFSGCTVHFVNDEVDGGAIIAQEIVEV
NFEDTPESLQKKVLEKEHILLPRIVKYLCEEKIEIHNGKVKILN
>Mature_204_residues
MYKIAVLASGSGSNLQSILDNINNGNIKGEVSLVIGSKEDIFALERAEKQGIKTSVVSKKEFGDKTSDEILRLAKENNIH
LIVLAGYLSILKGKLLEEYGNRIINIHPSLIPSFCGNKMYGINVHKAAIEKGVKFSGCTVHFVNDEVDGGAIIAQEIVEV
NFEDTPESLQKKVLEKEHILLPRIVKYLCEEKIEIHNGKVKILN

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=201, Percent_Identity=37.3134328358209, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI209869995, Length=201, Percent_Identity=37.3134328358209, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI209869993, Length=201, Percent_Identity=37.3134328358209, Blast_Score=143, Evalue=1e-34,
Organism=Escherichia coli, GI1788846, Length=195, Percent_Identity=35.3846153846154, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1787483, Length=206, Percent_Identity=28.6407766990291, Blast_Score=91, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=35.5555555555556, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320616, Length=200, Percent_Identity=30.5, Blast_Score=76, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24582400, Length=190, Percent_Identity=40, Blast_Score=139, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22539; Mature: 22539

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKIAVLASGSGSNLQSILDNINNGNIKGEVSLVIGSKEDIFALERAEKQGIKTSVVSKK
CEEEEEEECCCCCCHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCHHHHHHHH
EFGDKTSDEILRLAKENNIHLIVLAGYLSILKGKLLEEYGNRIINIHPSLIPSFCGNKMY
HCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHCCEEE
GINVHKAAIEKGVKFSGCTVHFVNDEVDGGAIIAQEIVEVNFEDTPESLQKKVLEKEHIL
ECCHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCH
LPRIVKYLCEEKIEIHNGKVKILN
HHHHHHHHHHCCCEECCCEEEEEC
>Mature Secondary Structure
MYKIAVLASGSGSNLQSILDNINNGNIKGEVSLVIGSKEDIFALERAEKQGIKTSVVSKK
CEEEEEEECCCCCCHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCHHHHHHHH
EFGDKTSDEILRLAKENNIHLIVLAGYLSILKGKLLEEYGNRIINIHPSLIPSFCGNKMY
HCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHCCEEE
GINVHKAAIEKGVKFSGCTVHFVNDEVDGGAIIAQEIVEVNFEDTPESLQKKVLEKEHIL
ECCHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCH
LPRIVKYLCEEKIEIHNGKVKILN
HHHHHHHHHHCCCEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]