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The map label for this gene is purH [H]

Identifier: 18309668

GI number: 18309668

Start: 853720

End: 855225

Strand: Direct

Name: purH [H]

Synonym: CPE0686

Alternate gene names: 18309668

Gene position: 853720-855225 (Clockwise)

Preceding gene: 18309667

Following gene: 18309669

Centisome position: 28.16

GC content: 30.94

Gene sequence:

>1506_bases
ATGAAAAAGAGAGCTTTAATAAGTGTTTTTGATAAAGATGGAGTTTTAGAACTTGCAAAATTTTTAAGAGATAGAGATGT
AGAAATAATATCAAGTGGTGGAACTTATAAATATTTAAAAGAAAATAACATAGAGGTTAAAGAGATAAGTGAAATAACTG
ACTTCCCAGAAATGTTAGATGGAAGAGTAAAAACTCTACATCCTTTAGTTCATGCTGGAATATTAGCAATAAGAGATAAT
AAAGAGCATATGAAAACTTTAGAAGAGAGAGAAATAAATACTATAGATTATGTAGTTGTAAATTTATATCCTTTCTTTGA
AAAAGTTAGAGAGGATTTAAGCTTTGAAGAAAAGGTTGAGTTTATTGATATAGGTGGACCAACAATGTTAAGAGCAGCAG
CTAAGAATTTTAAAGATGTGGTAGTTCTTTCAGATAAAAAAGATTATGAAAAAGTAATGAATGAAATTAAAGAAAATAAT
TGTGTTTCATTTAAATTAAGAAAAACTCTTGCAGGAAAAGTATTTAACTTAATGAGTGCTTATGATGCTGCAATTTCTAA
CTTCTTACTAGAGGGAGAAGAGGAATATCCAGAATATCTATCAGTTTCATATAAGAAAATTCAAGATCTTAGATATGGAG
AAAATCCACATCAAGGAGCTGCTTATTATAGTTCAACAGAATTTGATGGAGCTATGAATAGCTTTGAAATTTTAAATGGA
AAAGCTTTATCTTACAATAATATAAAGGATTTAGATATAGCATGGAAGGTTGCTTGTGAATTTGAAGAAACAGCTTGCTG
TGCATTAAAACATAATACACCTTGTGGGGTAGCTGTAGGAGAAAATTCTAAAGAGGTTTATTTAAAGGCTTATGATGCTG
ATCCAGTTTCAATATTTGGTGGAATAGTTGCTATAAATAGAAAGATAGATAAGGCTACAGCAGAAGAAATGGTTAAAATT
TTCTTAGAGGTGGTAGCAGCACCAGATTTTGATGAAGATGCTTTAGAAGTATTAAGAACTAAAAAGAATTTAAGAGTTAT
AAAATGTAAGAATACTCCTCAGGCTAAAAATTACATGGTAACAGTAGATGGTGGAATATTAGTTCAAGGAGAAGATAATA
AACTTGCTAATGAGTATAAAGTTGTAACTGAAAAAGAGCCAACAGAAATGGAATTAAGAGATATGATCTTTGGAATGAAG
GTTGTGAAATATGTAAAATCTAATGCTATAGTAGTTGTAAAAGATGGAGTTGCCACTGGTATTGGTGGAGGACAAGTTAA
TAGAATTTGGGCTACTAAAGAAGCTTTAGAAAGAGGAAAAGGTGGAGCCGTTTTAGCATCAGATGCTTTCTTCCCATTTA
GAGACTGTGTTGACGAAGCGGCTAAAAATGGAATAAAAGCAATAATTCAACCAGGTGGATCTATTAGAGATGAGGAGTCT
ATAGAGGCTTGTAATGAACATGGAATAAGCATGGTATTTACAGGAGTTAGACATTTTAAACATTAG

Upstream 100 bases:

>100_bases
GGAGCATATATTGCTACCTAGAATTGTTAAGTACTTATGCGAAGAAAAAATAGAAATTCATAATGGAAAAGTGAAAATAT
TAAACTAGGGAGGTCTTTTA

Downstream 100 bases:

>100_bases
AAATGAATAGATTTTAGGAGGTGAATTAGGTCATATAGTTAATTTGAATTTTAAATAGTTAATTATATAAGTTAAAACTA
TTTAAACTAAGTTTAATTTA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MKKRALISVFDKDGVLELAKFLRDRDVEIISSGGTYKYLKENNIEVKEISEITDFPEMLDGRVKTLHPLVHAGILAIRDN
KEHMKTLEEREINTIDYVVVNLYPFFEKVREDLSFEEKVEFIDIGGPTMLRAAAKNFKDVVVLSDKKDYEKVMNEIKENN
CVSFKLRKTLAGKVFNLMSAYDAAISNFLLEGEEEYPEYLSVSYKKIQDLRYGENPHQGAAYYSSTEFDGAMNSFEILNG
KALSYNNIKDLDIAWKVACEFEETACCALKHNTPCGVAVGENSKEVYLKAYDADPVSIFGGIVAINRKIDKATAEEMVKI
FLEVVAAPDFDEDALEVLRTKKNLRVIKCKNTPQAKNYMVTVDGGILVQGEDNKLANEYKVVTEKEPTEMELRDMIFGMK
VVKYVKSNAIVVVKDGVATGIGGGQVNRIWATKEALERGKGGAVLASDAFFPFRDCVDEAAKNGIKAIIQPGGSIRDEES
IEACNEHGISMVFTGVRHFKH

Sequences:

>Translated_501_residues
MKKRALISVFDKDGVLELAKFLRDRDVEIISSGGTYKYLKENNIEVKEISEITDFPEMLDGRVKTLHPLVHAGILAIRDN
KEHMKTLEEREINTIDYVVVNLYPFFEKVREDLSFEEKVEFIDIGGPTMLRAAAKNFKDVVVLSDKKDYEKVMNEIKENN
CVSFKLRKTLAGKVFNLMSAYDAAISNFLLEGEEEYPEYLSVSYKKIQDLRYGENPHQGAAYYSSTEFDGAMNSFEILNG
KALSYNNIKDLDIAWKVACEFEETACCALKHNTPCGVAVGENSKEVYLKAYDADPVSIFGGIVAINRKIDKATAEEMVKI
FLEVVAAPDFDEDALEVLRTKKNLRVIKCKNTPQAKNYMVTVDGGILVQGEDNKLANEYKVVTEKEPTEMELRDMIFGMK
VVKYVKSNAIVVVKDGVATGIGGGQVNRIWATKEALERGKGGAVLASDAFFPFRDCVDEAAKNGIKAIIQPGGSIRDEES
IEACNEHGISMVFTGVRHFKH
>Mature_501_residues
MKKRALISVFDKDGVLELAKFLRDRDVEIISSGGTYKYLKENNIEVKEISEITDFPEMLDGRVKTLHPLVHAGILAIRDN
KEHMKTLEEREINTIDYVVVNLYPFFEKVREDLSFEEKVEFIDIGGPTMLRAAAKNFKDVVVLSDKKDYEKVMNEIKENN
CVSFKLRKTLAGKVFNLMSAYDAAISNFLLEGEEEYPEYLSVSYKKIQDLRYGENPHQGAAYYSSTEFDGAMNSFEILNG
KALSYNNIKDLDIAWKVACEFEETACCALKHNTPCGVAVGENSKEVYLKAYDADPVSIFGGIVAINRKIDKATAEEMVKI
FLEVVAAPDFDEDALEVLRTKKNLRVIKCKNTPQAKNYMVTVDGGILVQGEDNKLANEYKVVTEKEPTEMELRDMIFGMK
VVKYVKSNAIVVVKDGVATGIGGGQVNRIWATKEALERGKGGAVLASDAFFPFRDCVDEAAKNGIKAIIQPGGSIRDEES
IEACNEHGISMVFTGVRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=465, Percent_Identity=36.5591397849462, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI1790439, Length=526, Percent_Identity=45.8174904942966, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI71985564, Length=479, Percent_Identity=35.4906054279749, Blast_Score=228, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI71985574, Length=307, Percent_Identity=28.6644951140065, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71985556, Length=62, Percent_Identity=51.6129032258064, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=467, Percent_Identity=35.9743040685225, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6323056, Length=471, Percent_Identity=36.5180467091295, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24649832, Length=468, Percent_Identity=33.7606837606838, Blast_Score=227, Evalue=2e-59,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56126; Mature: 56126

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRALISVFDKDGVLELAKFLRDRDVEIISSGGTYKYLKENNIEVKEISEITDFPEMLD
CCCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHHHCCHHHHC
GRVKTLHPLVHAGILAIRDNKEHMKTLEEREINTIDYVVVNLYPFFEKVREDLSFEEKVE
CHHHHHHHHHHHCEEEEECCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCHHHCCC
FIDIGGPTMLRAAAKNFKDVVVLSDKKDYEKVMNEIKENNCVSFKLRKTLAGKVFNLMSA
EEECCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHH
YDAAISNFLLEGEEEYPEYLSVSYKKIQDLRYGENPHQGAAYYSSTEFDGAMNSFEILNG
HHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEECC
KALSYNNIKDLDIAWKVACEFEETACCALKHNTPCGVAVGENSKEVYLKAYDADPVSIFG
CEECCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCEEECCCCCEEEEEEECCCHHHHHC
GIVAINRKIDKATAEEMVKIFLEVVAAPDFDEDALEVLRTKKNLRVIKCKNTPQAKNYMV
CCEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCEEE
TVDGGILVQGEDNKLANEYKVVTEKEPTEMELRDMIFGMKVVKYVKSNAIVVVKDGVATG
EECCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEC
IGGGQVNRIWATKEALERGKGGAVLASDAFFPFRDCVDEAAKNGIKAIIQPGGSIRDEES
CCCCCEEEEEECHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHH
IEACNEHGISMVFTGVRHFKH
HHHHHHCCCHHEEHHHHHHCC
>Mature Secondary Structure
MKKRALISVFDKDGVLELAKFLRDRDVEIISSGGTYKYLKENNIEVKEISEITDFPEMLD
CCCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHHHCCHHHHC
GRVKTLHPLVHAGILAIRDNKEHMKTLEEREINTIDYVVVNLYPFFEKVREDLSFEEKVE
CHHHHHHHHHHHCEEEEECCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCHHHCCC
FIDIGGPTMLRAAAKNFKDVVVLSDKKDYEKVMNEIKENNCVSFKLRKTLAGKVFNLMSA
EEECCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHH
YDAAISNFLLEGEEEYPEYLSVSYKKIQDLRYGENPHQGAAYYSSTEFDGAMNSFEILNG
HHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEECC
KALSYNNIKDLDIAWKVACEFEETACCALKHNTPCGVAVGENSKEVYLKAYDADPVSIFG
CEECCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCEEECCCCCEEEEEEECCCHHHHHC
GIVAINRKIDKATAEEMVKIFLEVVAAPDFDEDALEVLRTKKNLRVIKCKNTPQAKNYMV
CCEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCEEE
TVDGGILVQGEDNKLANEYKVVTEKEPTEMELRDMIFGMKVVKYVKSNAIVVVKDGVATG
EECCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEC
IGGGQVNRIWATKEALERGKGGAVLASDAFFPFRDCVDEAAKNGIKAIIQPGGSIRDEES
CCCCCEEEEEECHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHH
IEACNEHGISMVFTGVRHFKH
HHHHHHCCCHHEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA