Click here to switch to the map view.

The map label for this gene is purF [H]

Identifier: 18309665

GI number: 18309665

Start: 850658

End: 852103

Strand: Direct

Name: purF [H]

Synonym: CPE0683

Alternate gene names: 18309665

Gene position: 850658-852103 (Clockwise)

Preceding gene: 18309664

Following gene: 18309666

Centisome position: 28.06

GC content: 34.23

Gene sequence:

>1446_bases
ATGGTGAAGCCAAATTTTGATATTATGGATCCACAAAATGATAAATTCAAAGATGAATGCGGAGTTTTTGGAGTTTTCGC
AAATAAACCAATAGATGTTGCTTCTATAAATTATTATGGACTTTATGCTCTTCAACATAGAGGACAAGAAAGTGCTGGCA
TTGCAGTAGCAAATGGAGAAGATATAAAAGTTCATAAGGGGTTAGGTGTTTTAACGGAAGCCTTTGAAGCTGAAGATTTA
AAGAGACTTAGAGAATTTAATGGTTATATATCAATAGGACATGTTAGATATTCTACAGCGGGAGCTAAAACTGTTGAAAA
TGCACAACCTTTAGTTAGTAATACTAAACTTGGTCCTATATCTACTGCTCATAATGGTAATTTAGTTAATGCAGATGTAA
TAAGAAGTCTTTTAGAGGATGGGGGACAAGTTTTCCATACTAGTGTTGATTCTGAGGTGATTACAAGCTTAGTTGCTAGA
GGAGCTAAAAAAGGGATTGAGAGGGCTGTAATTGATGCTATATCAGCGGTTAAAGGTTCTTTCGCTATGGTAATAATGAC
TAAGGATAAGCTAATAGGTATAAGAGATCCACATGGAATTAGACCTTTATGCTTAGGAAAATTTGAAGAGGGATATATAT
TAACTTCAGAAAGTTGTGCTTTAGACACAATTGGAGCAGAGTTTGTGAGAGATATAAAACCAGGAGAAATCGTGGTTATA
GATAATGATGGGATTAAAAGTTATAGATATTCAGAAAATACTGTTTGCCAAACCTGTGCCTTTGAATATATATACTTTGC
AAGACCAGATTCAGTAATTGATGGTTTAGATGTTCAAACTACAAGGGTTAAGCAAGGAGAAATATTATTTAAAGAATATC
CAATAGATGCTGATATTGTGGTAGCTGTACCAGATTCAGGAATACCAGCTGCTATGGGATATGCTAAGGCTTCAGGAATT
CCTTATGAGGTTGGATTTGTGAAAAATAGATATATAGGAAGAACATTTATATCACCATCACAAGAAATTAGGGAGAGAGC
TGTAGCTGTAAAGCTTAATCCTTTAAAAGTTAATGTAAATGGAAAGAGAGTTATTTTAATAGACGATTCTATTGTTAGAG
GAACAACTTCTAAACATTTAATTGAATCTTTAAGAAGAGCTGGTGCTAAGGAAGTTCATTTTCTAGTTGCATCACCAATG
GTTAAATATCCATGTTATTTTGGCATTGATACACCATATAGAAGTCAGTTAATTGGAGCAAGTAGAAGTGTAGAAGAAAT
TAGAGAGATGATAGGTTGTGATTCTCTAGGGTATTTAAGTTTAGAAGGAATGTACGAAAGTTTTGAAGGTAGAAGAAATT
TCTGTGTAGGATGCTTTAGTGGGGTTTATCCAGTTGCAGCGCCTATGGAAGCTTTAGAGGATAATCTTGAAAGGGTGGAG
AATTAA

Upstream 100 bases:

>100_bases
GGACAAAGACAGATTTAGAAGAGATATGGGGAATGTAATCAATGGCTATAGAGAGGTTTTAAATAGACTAAGAAATTAGG
TAGTAAGAGGAGTAGAAAAT

Downstream 100 bases:

>100_bases
ATGCTAACTTACAAAGAAGCAGGAGTAAATATTGAAGAAGGATATAGATCAGTAAAATTAATAAAAGAATATGCAAAAAA
AACAATGAGTGAATATGTAT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MVKPNFDIMDPQNDKFKDECGVFGVFANKPIDVASINYYGLYALQHRGQESAGIAVANGEDIKVHKGLGVLTEAFEAEDL
KRLREFNGYISIGHVRYSTAGAKTVENAQPLVSNTKLGPISTAHNGNLVNADVIRSLLEDGGQVFHTSVDSEVITSLVAR
GAKKGIERAVIDAISAVKGSFAMVIMTKDKLIGIRDPHGIRPLCLGKFEEGYILTSESCALDTIGAEFVRDIKPGEIVVI
DNDGIKSYRYSENTVCQTCAFEYIYFARPDSVIDGLDVQTTRVKQGEILFKEYPIDADIVVAVPDSGIPAAMGYAKASGI
PYEVGFVKNRYIGRTFISPSQEIRERAVAVKLNPLKVNVNGKRVILIDDSIVRGTTSKHLIESLRRAGAKEVHFLVASPM
VKYPCYFGIDTPYRSQLIGASRSVEEIREMIGCDSLGYLSLEGMYESFEGRRNFCVGCFSGVYPVAAPMEALEDNLERVE
N

Sequences:

>Translated_481_residues
MVKPNFDIMDPQNDKFKDECGVFGVFANKPIDVASINYYGLYALQHRGQESAGIAVANGEDIKVHKGLGVLTEAFEAEDL
KRLREFNGYISIGHVRYSTAGAKTVENAQPLVSNTKLGPISTAHNGNLVNADVIRSLLEDGGQVFHTSVDSEVITSLVAR
GAKKGIERAVIDAISAVKGSFAMVIMTKDKLIGIRDPHGIRPLCLGKFEEGYILTSESCALDTIGAEFVRDIKPGEIVVI
DNDGIKSYRYSENTVCQTCAFEYIYFARPDSVIDGLDVQTTRVKQGEILFKEYPIDADIVVAVPDSGIPAAMGYAKASGI
PYEVGFVKNRYIGRTFISPSQEIRERAVAVKLNPLKVNVNGKRVILIDDSIVRGTTSKHLIESLRRAGAKEVHFLVASPM
VKYPCYFGIDTPYRSQLIGASRSVEEIREMIGCDSLGYLSLEGMYESFEGRRNFCVGCFSGVYPVAAPMEALEDNLERVE
N
>Mature_481_residues
MVKPNFDIMDPQNDKFKDECGVFGVFANKPIDVASINYYGLYALQHRGQESAGIAVANGEDIKVHKGLGVLTEAFEAEDL
KRLREFNGYISIGHVRYSTAGAKTVENAQPLVSNTKLGPISTAHNGNLVNADVIRSLLEDGGQVFHTSVDSEVITSLVAR
GAKKGIERAVIDAISAVKGSFAMVIMTKDKLIGIRDPHGIRPLCLGKFEEGYILTSESCALDTIGAEFVRDIKPGEIVVI
DNDGIKSYRYSENTVCQTCAFEYIYFARPDSVIDGLDVQTTRVKQGEILFKEYPIDADIVVAVPDSGIPAAMGYAKASGI
PYEVGFVKNRYIGRTFISPSQEIRERAVAVKLNPLKVNVNGKRVILIDDSIVRGTTSKHLIESLRRAGAKEVHFLVASPM
VKYPCYFGIDTPYRSQLIGASRSVEEIREMIGCDSLGYLSLEGMYESFEGRRNFCVGCFSGVYPVAAPMEALEDNLERVE
N

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=517, Percent_Identity=38.6847195357834, Blast_Score=353, Evalue=2e-97,
Organism=Escherichia coli, GI1788651, Length=489, Percent_Identity=38.4458077709611, Blast_Score=321, Evalue=8e-89,
Organism=Escherichia coli, GI1790167, Length=378, Percent_Identity=26.1904761904762, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17554892, Length=482, Percent_Identity=41.2863070539419, Blast_Score=341, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI17532897, Length=184, Percent_Identity=28.2608695652174, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532899, Length=184, Percent_Identity=28.2608695652174, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323958, Length=502, Percent_Identity=37.6494023904382, Blast_Score=275, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24659598, Length=491, Percent_Identity=40.3258655804481, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI28573187, Length=497, Percent_Identity=40.6438631790744, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24659604, Length=466, Percent_Identity=40.7725321888412, Blast_Score=353, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52726; Mature: 52726

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKPNFDIMDPQNDKFKDECGVFGVFANKPIDVASINYYGLYALQHRGQESAGIAVANGE
CCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCEEEECEEEEEEEECCCCCCCCEEEECCC
DIKVHKGLGVLTEAFEAEDLKRLREFNGYISIGHVRYSTAGAKTVENAQPLVSNTKLGPI
EEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHCCCCCCCCCCCCCCC
STAHNGNLVNADVIRSLLEDGGQVFHTSVDSEVITSLVARGAKKGIERAVIDAISAVKGS
CCCCCCCEECHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FAMVIMTKDKLIGIRDPHGIRPLCLGKFEEGYILTSESCALDTIGAEFVRDIKPGEIVVI
EEEEEEECCCEEECCCCCCCCCCEECCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEE
DNDGIKSYRYSENTVCQTCAFEYIYFARPDSVIDGLDVQTTRVKQGEILFKEYPIDADIV
ECCCCCCEECCCCCHHHHHHEEEEEEECCCHHHCCCCCEEEEECCCCEEEEECCCCCEEE
VAVPDSGIPAAMGYAKASGIPYEVGFVKNRYIGRTFISPSQEIRERAVAVKLNPLKVNVN
EEECCCCCCHHHCHHHHCCCCEEECEECCCCCCCEECCCHHHHHHCEEEEEEEEEEEEEC
GKRVILIDDSIVRGTTSKHLIESLRRAGAKEVHFLVASPMVKYPCYFGIDTPYRSQLIGA
CCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHCCEEECCCCCHHHHHHCC
SRSVEEIREMIGCDSLGYLSLEGMYESFEGRRNFCVGCFSGVYPVAAPMEALEDNLERVE
CCCHHHHHHHHCCCCCCEEEEHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
N
C
>Mature Secondary Structure
MVKPNFDIMDPQNDKFKDECGVFGVFANKPIDVASINYYGLYALQHRGQESAGIAVANGE
CCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCEEEECEEEEEEEECCCCCCCCEEEECCC
DIKVHKGLGVLTEAFEAEDLKRLREFNGYISIGHVRYSTAGAKTVENAQPLVSNTKLGPI
EEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHCCCCCCCCCCCCCCC
STAHNGNLVNADVIRSLLEDGGQVFHTSVDSEVITSLVARGAKKGIERAVIDAISAVKGS
CCCCCCCEECHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FAMVIMTKDKLIGIRDPHGIRPLCLGKFEEGYILTSESCALDTIGAEFVRDIKPGEIVVI
EEEEEEECCCEEECCCCCCCCCCEECCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEE
DNDGIKSYRYSENTVCQTCAFEYIYFARPDSVIDGLDVQTTRVKQGEILFKEYPIDADIV
ECCCCCCEECCCCCHHHHHHEEEEEEECCCHHHCCCCCEEEEECCCCEEEEECCCCCEEE
VAVPDSGIPAAMGYAKASGIPYEVGFVKNRYIGRTFISPSQEIRERAVAVKLNPLKVNVN
EEECCCCCCHHHCHHHHCCCCEEECEECCCCCCCEECCCHHHHHHCEEEEEEEEEEEEEC
GKRVILIDDSIVRGTTSKHLIESLRRAGAKEVHFLVASPMVKYPCYFGIDTPYRSQLIGA
CCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHCCEEECCCCCHHHHHHCC
SRSVEEIREMIGCDSLGYLSLEGMYESFEGRRNFCVGCFSGVYPVAAPMEALEDNLERVE
CCCHHHHHHHHCCCCCCEEEEHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809759 [H]