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The map label for this gene is rfbB [H]

Identifier: 18309601

GI number: 18309601

Start: 774940

End: 775989

Strand: Direct

Name: rfbB [H]

Synonym: CPE0619

Alternate gene names: 18309601

Gene position: 774940-775989 (Clockwise)

Preceding gene: 18309600

Following gene: 18309602

Centisome position: 25.56

GC content: 29.71

Gene sequence:

>1050_bases
ATGAAAACTTACTTAGTTACAGGTGGAGCAGGATTTATAGGATCTAACTTTGTATTATATATGTTAAAGAAATACAAAGA
TATAAAAATAATAAACTTAGATAAATTAACTTATGCAGGAAATTTAGAAAACTTAAAATCAATAGAAAATGATGAAAGAC
ATATATTCGTTCAAGGAGATATATGTGATAAAGAATTAGTTTCAAGTTTATTTGAAAAATATGAGATAGACTATGTAGCT
CACTTTGCAGCAGAATCACATGTTGATAGAAGTATAAGAGAGCCAGAAGTATTTGCTAAAACTAATGTATTAGGAACAGT
AAATTTACTTAACTGTGCTAAAAATGCATGGGAAACAGAAGAAGGATGGAAAGAAGGAGTTAAATTCCTGCATGTTTCAA
CAGATGAAGTTTATGGTTCATTAGGAGAAACAGGATATTTCATGGAAACAACACCATTAGATCCACATAGTCCATATTCA
TCAAGCAAAGCAAGTTCAGATTTAATGGTTAAGGCTTATGCAGATACATATAAAATGCCAGTAAACATAACAAGATGTTC
AAATAACTATGGACCATTCCAATTCCCAGAAAAACTTATACCACTTCTAATAAATAACTGTTTAAATCATAAGGATTTAC
CAGTTTACGGAGATGGAATGAATATAAGAGACTGGTTATTCGTTGAAGACCATGCAAAAGCAATCGATATGGTAATAAAC
GGGGGAAGACTTGGAGAAGTTTACAATGTTGGTGGACATAATGAAAGAACTAACATTCAAATAGTAAAAACAGTTATAGA
ATACTTACATGATAATGTAGATGAAGAAATAACTGAAAACTTAATAAAGTATGTTGAAGATAGAAAAGGTCATGATAGAA
GATACGGAATAGATCCAACAAAGATAAAAGAAGAGTTAGGATGGTATCCAGAAACAACTTTTGAAGTTGGAATAAAGAAA
ACAATAAAATGGTACTTAGATAATAAAGAATGGATGGAAAATGTAACATCAGGAGATTATCAAAAATATTATGAGAAGAT
GTATAGATAA

Upstream 100 bases:

>100_bases
TACAATTGGTGATGAAATGAGAGATTGGAAGGAAGCTTTAAAAAGCTTTTTTGAAAATCAACAAAATCAATAAAAGTTAT
TAAAGTTAGGAGAGAATTAA

Downstream 100 bases:

>100_bases
TAAATTGAATATAGGAGATAATATATGAATTTAAGAAATAAATCAAACACAAAGTATGAACTTTTTATATATTTTGTGCT
ATTCTCAATATTTATTAGAG

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKTYLVTGGAGFIGSNFVLYMLKKYKDIKIINLDKLTYAGNLENLKSIENDERHIFVQGDICDKELVSSLFEKYEIDYVA
HFAAESHVDRSIREPEVFAKTNVLGTVNLLNCAKNAWETEEGWKEGVKFLHVSTDEVYGSLGETGYFMETTPLDPHSPYS
SSKASSDLMVKAYADTYKMPVNITRCSNNYGPFQFPEKLIPLLINNCLNHKDLPVYGDGMNIRDWLFVEDHAKAIDMVIN
GGRLGEVYNVGGHNERTNIQIVKTVIEYLHDNVDEEITENLIKYVEDRKGHDRRYGIDPTKIKEELGWYPETTFEVGIKK
TIKWYLDNKEWMENVTSGDYQKYYEKMYR

Sequences:

>Translated_349_residues
MKTYLVTGGAGFIGSNFVLYMLKKYKDIKIINLDKLTYAGNLENLKSIENDERHIFVQGDICDKELVSSLFEKYEIDYVA
HFAAESHVDRSIREPEVFAKTNVLGTVNLLNCAKNAWETEEGWKEGVKFLHVSTDEVYGSLGETGYFMETTPLDPHSPYS
SSKASSDLMVKAYADTYKMPVNITRCSNNYGPFQFPEKLIPLLINNCLNHKDLPVYGDGMNIRDWLFVEDHAKAIDMVIN
GGRLGEVYNVGGHNERTNIQIVKTVIEYLHDNVDEEITENLIKYVEDRKGHDRRYGIDPTKIKEELGWYPETTFEVGIKK
TIKWYLDNKEWMENVTSGDYQKYYEKMYR
>Mature_349_residues
MKTYLVTGGAGFIGSNFVLYMLKKYKDIKIINLDKLTYAGNLENLKSIENDERHIFVQGDICDKELVSSLFEKYEIDYVA
HFAAESHVDRSIREPEVFAKTNVLGTVNLLNCAKNAWETEEGWKEGVKFLHVSTDEVYGSLGETGYFMETTPLDPHSPYS
SSKASSDLMVKAYADTYKMPVNITRCSNNYGPFQFPEKLIPLLINNCLNHKDLPVYGDGMNIRDWLFVEDHAKAIDMVIN
GGRLGEVYNVGGHNERTNIQIVKTVIEYLHDNVDEEITENLIKYVEDRKGHDRRYGIDPTKIKEELGWYPETTFEVGIKK
TIKWYLDNKEWMENVTSGDYQKYYEKMYR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=335, Percent_Identity=42.3880597014925, Blast_Score=258, Evalue=5e-69,
Organism=Homo sapiens, GI42516563, Length=339, Percent_Identity=24.188790560472, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI56237023, Length=362, Percent_Identity=26.7955801104972, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI56118217, Length=362, Percent_Identity=26.7955801104972, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI189083684, Length=362, Percent_Identity=26.7955801104972, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4504031, Length=341, Percent_Identity=24.9266862170088, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=50.9859154929577, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=51.2747875354108, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1786974, Length=346, Percent_Identity=26.878612716763, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788366, Length=353, Percent_Identity=25.4957507082153, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=325, Percent_Identity=38.1538461538462, Blast_Score=218, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI115532424, Length=330, Percent_Identity=33.6363636363636, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17539532, Length=332, Percent_Identity=25.9036144578313, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=361, Percent_Identity=25.7617728531856, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71982038, Length=363, Percent_Identity=25.6198347107438, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=243, Percent_Identity=27.9835390946502, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=243, Percent_Identity=27.9835390946502, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=243, Percent_Identity=27.9835390946502, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=243, Percent_Identity=27.9835390946502, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=243, Percent_Identity=27.9835390946502, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=243, Percent_Identity=27.5720164609054, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=355, Percent_Identity=27.6056338028169, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21356223, Length=332, Percent_Identity=26.5060240963855, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI19923002, Length=368, Percent_Identity=28.5326086956522, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24158427, Length=343, Percent_Identity=24.4897959183673, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40296; Mature: 40296

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYLVTGGAGFIGSNFVLYMLKKYKDIKIINLDKLTYAGNLENLKSIENDERHIFVQGD
CCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCEEECCCHHHHHHCCCCCCEEEEECC
ICDKELVSSLFEKYEIDYVAHFAAESHVDRSIREPEVFAKTNVLGTVNLLNCAKNAWETE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEHHHHHHHHHCCCCH
EGWKEGVKFLHVSTDEVYGSLGETGYFMETTPLDPHSPYSSSKASSDLMVKAYADTYKMP
HHHHHCCEEEEEEHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCEECC
VNITRCSNNYGPFQFPEKLIPLLINNCLNHKDLPVYGDGMNIRDWLFVEDHAKAIDMVIN
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEECHHHHEEEEEEC
GGRLGEVYNVGGHNERTNIQIVKTVIEYLHDNVDEEITENLIKYVEDRKGHDRRYGIDPT
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
KIKEELGWYPETTFEVGIKKTIKWYLDNKEWMENVTSGDYQKYYEKMYR
HHHHHHCCCCCCHHHHHHHHHHHHEECCHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKTYLVTGGAGFIGSNFVLYMLKKYKDIKIINLDKLTYAGNLENLKSIENDERHIFVQGD
CCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCEEECCCHHHHHHCCCCCCEEEEECC
ICDKELVSSLFEKYEIDYVAHFAAESHVDRSIREPEVFAKTNVLGTVNLLNCAKNAWETE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEHHHHHHHHHCCCCH
EGWKEGVKFLHVSTDEVYGSLGETGYFMETTPLDPHSPYSSSKASSDLMVKAYADTYKMP
HHHHHCCEEEEEEHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCEECC
VNITRCSNNYGPFQFPEKLIPLLINNCLNHKDLPVYGDGMNIRDWLFVEDHAKAIDMVIN
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEECHHHHEEEEEEC
GGRLGEVYNVGGHNERTNIQIVKTVIEYLHDNVDEEITENLIKYVEDRKGHDRRYGIDPT
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
KIKEELGWYPETTFEVGIKKTIKWYLDNKEWMENVTSGDYQKYYEKMYR
HHHHHHCCCCCCHHHHHHHHHHHHEECCHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]