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The map label for this gene is rfbD [H]

Identifier: 18309600

GI number: 18309600

Start: 774028

End: 774912

Strand: Direct

Name: rfbD [H]

Synonym: CPE0618

Alternate gene names: 18309600

Gene position: 774028-774912 (Clockwise)

Preceding gene: 18309599

Following gene: 18309601

Centisome position: 25.53

GC content: 29.04

Gene sequence:

>885_bases
ATGAAAATATTAATAACAGGTTCAAATGGACAATTAGGAAATGAACTTCAATCAATAATAAAAAGTGGAAAAGCAGAAAT
AGGTTCAGTTAGTGAAAATATAAAAAACTCAGAAGTTATTGCTTTAGATGTTGATAAATTAGATATAACTAGCTTAGAAA
ATGTTAAAAATGTACTTAATGAAGAAAAACCAGATGTAGTAATAAACTGTGCAGCGGCAACAAATGTTGACGGATGTGAA
AGTAATGAAGATTTTGCATTTAAAGTTAATTCTTTAGGAGCAAGAAATTTAGCTATGGTTTGTGAAGAAATTGGAGCTAA
ATTAGTTCAAGTTTCAACAGATTATGTATTTAGTGGTTTTGGTGAAAAACCATTAACTGAGTTTGATTTAACTGCACCTT
ATAGTGTTTATGGAAAAACAAAACTTTTAGGTGAAAATTATGTTAAAGAGTTTTGTTCAAAATATTATATAGTTAGAACC
GCTTGGTTATATGGATATGTGGGACATAACTTTGTTTACACAATGATGAGACTTGGAAAAGAAAAAGAATCATTAAATGT
AGTTAATGATCAAAAAGGAAATCCAACTCACGCAAATGATTTAGCATATCATATATTAAAGCTTATAGAAACAGAAGAAT
ACGGAATATACCATTGTACAGGAAAAGGGGAATGTACTTGGTATGATTTTGCTAAAGAAATAATGGAGTTAGCTGGCAAT
AAGTGCATTGTTAACCCATGTACTTCAGAAGAATATAAAACACCAGCTAAAAGACCAGAATATTCATCATTAGATAATAT
GATGCTTAGATGTACAATTGGTGATGAAATGAGAGATTGGAAGGAAGCTTTAAAAAGCTTTTTTGAAAATCAACAAAATC
AATAA

Upstream 100 bases:

>100_bases
GGAATAGAAGAAATAATCTTATCAGAAAAAGATAAGAAACAAAAAACTTTAAAAGAATTAGATATTCCTTTTATATATGA
AGGTTAATAGGAGGAAGTCT

Downstream 100 bases:

>100_bases
AAGTTATTAAAGTTAGGAGAGAATTAAATGAAAACTTACTTAGTTACAGGTGGAGCAGGATTTATAGGATCTAACTTTGT
ATTATATATGTTAAAGAAAT

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKILITGSNGQLGNELQSIIKSGKAEIGSVSENIKNSEVIALDVDKLDITSLENVKNVLNEEKPDVVINCAAATNVDGCE
SNEDFAFKVNSLGARNLAMVCEEIGAKLVQVSTDYVFSGFGEKPLTEFDLTAPYSVYGKTKLLGENYVKEFCSKYYIVRT
AWLYGYVGHNFVYTMMRLGKEKESLNVVNDQKGNPTHANDLAYHILKLIETEEYGIYHCTGKGECTWYDFAKEIMELAGN
KCIVNPCTSEEYKTPAKRPEYSSLDNMMLRCTIGDEMRDWKEALKSFFENQQNQ

Sequences:

>Translated_294_residues
MKILITGSNGQLGNELQSIIKSGKAEIGSVSENIKNSEVIALDVDKLDITSLENVKNVLNEEKPDVVINCAAATNVDGCE
SNEDFAFKVNSLGARNLAMVCEEIGAKLVQVSTDYVFSGFGEKPLTEFDLTAPYSVYGKTKLLGENYVKEFCSKYYIVRT
AWLYGYVGHNFVYTMMRLGKEKESLNVVNDQKGNPTHANDLAYHILKLIETEEYGIYHCTGKGECTWYDFAKEIMELAGN
KCIVNPCTSEEYKTPAKRPEYSSLDNMMLRCTIGDEMRDWKEALKSFFENQQNQ
>Mature_294_residues
MKILITGSNGQLGNELQSIIKSGKAEIGSVSENIKNSEVIALDVDKLDITSLENVKNVLNEEKPDVVINCAAATNVDGCE
SNEDFAFKVNSLGARNLAMVCEEIGAKLVQVSTDYVFSGFGEKPLTEFDLTAPYSVYGKTKLLGENYVKEFCSKYYIVRT
AWLYGYVGHNFVYTMMRLGKEKESLNVVNDQKGNPTHANDLAYHILKLIETEEYGIYHCTGKGECTWYDFAKEIMELAGN
KCIVNPCTSEEYKTPAKRPEYSSLDNMMLRCTIGDEMRDWKEALKSFFENQQNQ

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=274, Percent_Identity=28.8321167883212, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI11034825, Length=274, Percent_Identity=28.8321167883212, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1788352, Length=305, Percent_Identity=38.0327868852459, Blast_Score=198, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 33034; Mature: 33034

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILITGSNGQLGNELQSIIKSGKAEIGSVSENIKNSEVIALDVDKLDITSLENVKNVLN
CEEEEECCCCCCHHHHHHHHHCCCCHHCHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHC
EEKPDVVINCAAATNVDGCESNEDFAFKVNSLGARNLAMVCEEIGAKLVQVSTDYVFSGF
CCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCHHHEEEEHHHHCCCC
GEKPLTEFDLTAPYSVYGKTKLLGENYVKEFCSKYYIVRTAWLYGYVGHNFVYTMMRLGK
CCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EKESLNVVNDQKGNPTHANDLAYHILKLIETEEYGIYHCTGKGECTWYDFAKEIMELAGN
CHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHCCC
KCIVNPCTSEEYKTPAKRPEYSSLDNMMLRCTIGDEMRDWKEALKSFFENQQNQ
EEEECCCCCHHHCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKILITGSNGQLGNELQSIIKSGKAEIGSVSENIKNSEVIALDVDKLDITSLENVKNVLN
CEEEEECCCCCCHHHHHHHHHCCCCHHCHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHC
EEKPDVVINCAAATNVDGCESNEDFAFKVNSLGARNLAMVCEEIGAKLVQVSTDYVFSGF
CCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCHHHEEEEHHHHCCCC
GEKPLTEFDLTAPYSVYGKTKLLGENYVKEFCSKYYIVRTAWLYGYVGHNFVYTMMRLGK
CCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EKESLNVVNDQKGNPTHANDLAYHILKLIETEEYGIYHCTGKGECTWYDFAKEIMELAGN
CHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHCCC
KCIVNPCTSEEYKTPAKRPEYSSLDNMMLRCTIGDEMRDWKEALKSFFENQQNQ
EEEECCCCCHHHCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]