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The map label for this gene is yafC [C]

Identifier: 17548049

GI number: 17548049

Start: 3594672

End: 3595568

Strand: Direct

Name: yafC [C]

Synonym: RSc3332

Alternate gene names: 17548049

Gene position: 3594672-3595568 (Clockwise)

Preceding gene: 17548032

Following gene: 17548050

Centisome position: 96.72

GC content: 69.23

Gene sequence:

>897_bases
ATGGACAAGCTGCGCAGCATGGAGACCTTCGTCGCCGTGGTGGATGCCGGCAGCTTCACCGAGGCGGCGATGCAGCTGGA
GATGTCGGCGGTGATGGTCGGCAAGTACGTGCGCGAGCTGGAAACACGGCTGGGGGCGCGGTTGCTGCAACGCAGCACGC
GCCGCCAGAGCCTGACCGACGCCGGCCGCGTGTTCTATGAAGACGCCAAGCGCGTGCTGGAACAGGTACGCTGGGCCGAG
ACCTCGGTCGAGCGGCTGCGCGCGCAGCCCTCCGGCACGCTGCGGGTCAGCGCGCCCACCACCTTCGGCGCCTGCGTGAT
CGCGCCGCTGACGGCGACCTTCCAGCAGGCCCATCCGCAGGTGCGCGTGGAGCTGGACCTGAGCAACCGCATGGTCGACC
TGGCGGACGAAGGCTTCGACCTCGCCGTGCGCATCGGCGCGCTGGGCGATGCCGACCTGGTGGCGAAACCGCTGTGCGCG
TACCGGATGGTCATCAGCGCCTCGCCCGACTATCTCGCGCGCCACGGCCGGCCCGAAACGCCGGCCGACCTGCCGGCCCA
TCGCTGCCTGTCCCACATGGTGTGGAACAGCCGCAATGCGTGGCAGCTGTCCGGCTGGGATGGCACGACATCCTGGCGCG
ACGATCCGGCCTTCAGCTGCAACGACGGCCACGGCCTGCGGATGGCCGCGATCGCCGGTGCGGGGCTGCTGCTGCAGCCG
GAGGTGCTGGTGGCCGAGGACCTGGCGAGCGGCCGGCTGGTGCGCGTGCTGCAGACGTATGCGCCGCAGCCGCGGCCGAT
CCATCTCGTGTATCGGCAAGATCGGCGGCCTTTGCCGAAGTTAACGGCTTTTACGGCGCATTTGCTGGAGCAGGTACCAG
CAAGAGGATTGACCTAG

Upstream 100 bases:

>100_bases
TCCTTTGCGAATCAGTTTGGAATGACACGCATTGTTGGCCGAACCCACAGAGCGATAAAGCCGTGGTAACTTTGTCTATT
CCTAATCAGCAGTTCGGGAT

Downstream 100 bases:

>100_bases
CGCAGCGGCAAGACGAACTGTGCCGGCGAGCGCTCCTCCTAGGCCAACGCCGGATCTCGGCTAAGCTAGCGGCCTCGGCT
TTCCTCTAGGAGGATCGTGA

Product: transcription regulator protein

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MDKLRSMETFVAVVDAGSFTEAAMQLEMSAVMVGKYVRELETRLGARLLQRSTRRQSLTDAGRVFYEDAKRVLEQVRWAE
TSVERLRAQPSGTLRVSAPTTFGACVIAPLTATFQQAHPQVRVELDLSNRMVDLADEGFDLAVRIGALGDADLVAKPLCA
YRMVISASPDYLARHGRPETPADLPAHRCLSHMVWNSRNAWQLSGWDGTTSWRDDPAFSCNDGHGLRMAAIAGAGLLLQP
EVLVAEDLASGRLVRVLQTYAPQPRPIHLVYRQDRRPLPKLTAFTAHLLEQVPARGLT

Sequences:

>Translated_298_residues
MDKLRSMETFVAVVDAGSFTEAAMQLEMSAVMVGKYVRELETRLGARLLQRSTRRQSLTDAGRVFYEDAKRVLEQVRWAE
TSVERLRAQPSGTLRVSAPTTFGACVIAPLTATFQQAHPQVRVELDLSNRMVDLADEGFDLAVRIGALGDADLVAKPLCA
YRMVISASPDYLARHGRPETPADLPAHRCLSHMVWNSRNAWQLSGWDGTTSWRDDPAFSCNDGHGLRMAAIAGAGLLLQP
EVLVAEDLASGRLVRVLQTYAPQPRPIHLVYRQDRRPLPKLTAFTAHLLEQVPARGLT
>Mature_298_residues
MDKLRSMETFVAVVDAGSFTEAAMQLEMSAVMVGKYVRELETRLGARLLQRSTRRQSLTDAGRVFYEDAKRVLEQVRWAE
TSVERLRAQPSGTLRVSAPTTFGACVIAPLTATFQQAHPQVRVELDLSNRMVDLADEGFDLAVRIGALGDADLVAKPLCA
YRMVISASPDYLARHGRPETPADLPAHRCLSHMVWNSRNAWQLSGWDGTTSWRDDPAFSCNDGHGLRMAAIAGAGLLLQP
EVLVAEDLASGRLVRVLQTYAPQPRPIHLVYRQDRRPLPKLTAFTAHLLEQVPARGLT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=34.3859649122807, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1789639, Length=281, Percent_Identity=36.2989323843416, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI145693193, Length=278, Percent_Identity=33.0935251798561, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI87081978, Length=272, Percent_Identity=33.8235294117647, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1789440, Length=270, Percent_Identity=30.3703703703704, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=29.3103448275862, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1787128, Length=276, Percent_Identity=27.536231884058, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788706, Length=299, Percent_Identity=27.7591973244147, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786448, Length=299, Percent_Identity=25.752508361204, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=26.530612244898, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788481, Length=170, Percent_Identity=28.2352941176471, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI157672245, Length=157, Percent_Identity=31.2101910828025, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787601, Length=135, Percent_Identity=30.3703703703704, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081904, Length=189, Percent_Identity=26.984126984127, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788297, Length=145, Percent_Identity=29.6551724137931, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787879, Length=169, Percent_Identity=31.3609467455621, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32970; Mature: 32970

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLRSMETFVAVVDAGSFTEAAMQLEMSAVMVGKYVRELETRLGARLLQRSTRRQSLTD
CCHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRVFYEDAKRVLEQVRWAETSVERLRAQPSGTLRVSAPTTFGACVIAPLTATFQQAHPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCC
VRVELDLSNRMVDLADEGFDLAVRIGALGDADLVAKPLCAYRMVISASPDYLARHGRPET
EEEEEECCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
PADLPAHRCLSHMVWNSRNAWQLSGWDGTTSWRDDPAFSCNDGHGLRMAAIAGAGLLLQP
CCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCEEECC
EVLVAEDLASGRLVRVLQTYAPQPRPIHLVYRQDRRPLPKLTAFTAHLLEQVPARGLT
CHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDKLRSMETFVAVVDAGSFTEAAMQLEMSAVMVGKYVRELETRLGARLLQRSTRRQSLTD
CCHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRVFYEDAKRVLEQVRWAETSVERLRAQPSGTLRVSAPTTFGACVIAPLTATFQQAHPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCC
VRVELDLSNRMVDLADEGFDLAVRIGALGDADLVAKPLCAYRMVISASPDYLARHGRPET
EEEEEECCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
PADLPAHRCLSHMVWNSRNAWQLSGWDGTTSWRDDPAFSCNDGHGLRMAAIAGAGLLLQP
CCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCEEECC
EVLVAEDLASGRLVRVLQTYAPQPRPIHLVYRQDRRPLPKLTAFTAHLLEQVPARGLT
CHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]