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The map label for this gene is gidA [H]

Identifier: 17548045

GI number: 17548045

Start: 3589890

End: 3591833

Strand: Reverse

Name: gidA [H]

Synonym: RSc3328

Alternate gene names: 17548045

Gene position: 3591833-3589890 (Counterclockwise)

Preceding gene: 17548046

Following gene: 17548044

Centisome position: 96.65

GC content: 65.53

Gene sequence:

>1944_bases
ATGCTGTATCCAGTTGAATTCGATGTGATCGTGGTGGGCGGCGGACACGCCGGCACCGAGGCCGCCTTGGCGGCGGCACG
CATGGGCTGCCAGACCCTGCTGCTGACGCACAACATCGAGACCTTGGGGCAGATGAGTTGCAACCCGTCCATCGGCGGCA
TCGGTAAGGGGCACCTGGTCAAGGAAGTCGATGCGCTGGGTGGTGCCATGGCACTGGCCACCGACGAGGGTGGCATCCAG
TTTCGCATCCTCAATTCCAGCAAGGGGCCGGCCGTGCGCGCCACCCGTGCCCAGGCGGACCGCGTGCTGTACAAAGCCGC
CATCCGCCATCGCCTGGAAAACCAGCCGAACCTGATGCTGTTCCAGCAGGCCGTGGAAGATCTGCTGGTGGAGGGCGATC
GCGTGGTCGGTGCCGTCACCCAGGTTGGCGTGCGGTTTCGCGCACGTGCAGTGGTGCTCACGGCCGGGACCTTCCTCGAC
GGCAAGATCCACGTCGGCCTGAACAACTACACCGGCGGCCGCGCGGGCGATCCGGCGGCGGTGTCGCTGTCGGCGCGCCT
GAAAGAACTGAAGCTGCCGCAAGGGCGCCTGAAGACCGGCACGCCGCCCCGCATCGACGGGCGCACCATCGACTTCTCGG
TGCTGGAAGCGCAGCCCGGGGACCTCGATCCCGTGCCGGTGTTCTCTTTCCTCGGCCGCACCGACATGCATCCGCGCCAA
GTGCCGTGCTGGGTGACGCACACTAACGAGCGCACGCACGACATCATCCGCGCCGGGCTGAACCGTTCGCCGATGTACAC
CGGCGTGATCGAAGGGGTCGGGCCGCGCTACTGCCCGAGCATCGAAGACAAGATCCACCGCTTTGCCAGCAAGGACAGCC
ACCAGATCTTTCTGGAGCCGGAAGGGCTGACCACCAACGAGTTCTACCCGAACGGCGTATCGACGAGCCTGCCGTTCGAC
GTGCAGCTGGACCTGATCCACTCGATGCGCGGTCTCGAACATGCCCACATCCTGCGGCCCGGCTACGCCATCGAATATGA
CTATTTCGATCCGCGCGGGCTGAAGGCTTCGCTGGAGTCCAAGGCGATCGGCGGCCTGTTCTTCGCCGGCCAGATCAATG
GCACGACCGGCTACGAGGAGGCCGCTGCCCAGGGGCTGCTGGCTGGCATCAACGCCGGCTTGCAAGTGCAGGGCAAGGCA
GCCTGGACGCCACGCCGCGACCAGGCCTATCTGGGCGTGCTGGTTGACGACCTGATCACGCGCGGCGTGACGGAGCCGTA
CCGCATGTTCACCAGCCGCGCCGAATTCCGCCTGAGCCTGCGTGAGGACAACGCTGACATGCGCCTGACCGAAGCGGGGC
GCGATCTTGGTGTGGTCGACGATGCCCGCTGGGATGCATTCAACCGCAAGCGCGATGCTGTTTCACGTGAAACAGAGCGC
CTGAAGTCGACTTGGGTGAACCCGGCGATCCTTCCCGCAGCCGATGCGGAACCGGTGCTGGGCAAGGGAATCGAGCGCGA
ATACTCGCTGGCCGATCTGCTGCGCCGCCCGAACGTGACATACGAATCGCTGATGGGCATGCAGGGCGGCAAGTATGCGC
CCGACGCTCCGCTGGCCGATGAGCCGCTGCTGGCCGAACAGATTCGCGAGCAGATCGAGATCGGCATCAAATACCACGGC
TACATTGCCCGCCAGGCGGACGAAGTCGAGCGCCTGGGTGCCAACGAGAACACCCGGCTGCCGGCCGATTTCGACTACAA
GCAGGTGCGCGGGCTGTCGATCGAAGTCCAGCAGAAGCTGGCGCAGCACAAGCCTGAAACGATCGGCCAGGCATCGCGCA
TCTCCGGCATCACGCCCGCGGCCGTGTCGCTGCTGCTGGTGCACCTGAAGAAGGGTGTGCTGCGTGGCAAGGTCGGCCCG
CGTTCCGAAGGCGAGGCCGCTTGA

Upstream 100 bases:

>100_bases
ATTTTGTCCAGAATCGTCCCGTGTCGAACAGTGGGGTAGGTGGACGACTTATAATTCCGCTTCCCCAATTTTCCCGATGC
CTCCAAGCGAGGAGGAAGCC

Downstream 100 bases:

>100_bases
TGGCGAACACCGCGCTGGCCGACACCCGGTCGGAACTGGAAGCCGGCGCGCGCGCGCTCGGCCTGCCGCTCGAGGGCGTG
CAACTGGATCGCCTGCTGGC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MLYPVEFDVIVVGGGHAGTEAALAAARMGCQTLLLTHNIETLGQMSCNPSIGGIGKGHLVKEVDALGGAMALATDEGGIQ
FRILNSSKGPAVRATRAQADRVLYKAAIRHRLENQPNLMLFQQAVEDLLVEGDRVVGAVTQVGVRFRARAVVLTAGTFLD
GKIHVGLNNYTGGRAGDPAAVSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSVLEAQPGDLDPVPVFSFLGRTDMHPRQ
VPCWVTHTNERTHDIIRAGLNRSPMYTGVIEGVGPRYCPSIEDKIHRFASKDSHQIFLEPEGLTTNEFYPNGVSTSLPFD
VQLDLIHSMRGLEHAHILRPGYAIEYDYFDPRGLKASLESKAIGGLFFAGQINGTTGYEEAAAQGLLAGINAGLQVQGKA
AWTPRRDQAYLGVLVDDLITRGVTEPYRMFTSRAEFRLSLREDNADMRLTEAGRDLGVVDDARWDAFNRKRDAVSRETER
LKSTWVNPAILPAADAEPVLGKGIEREYSLADLLRRPNVTYESLMGMQGGKYAPDAPLADEPLLAEQIREQIEIGIKYHG
YIARQADEVERLGANENTRLPADFDYKQVRGLSIEVQQKLAQHKPETIGQASRISGITPAAVSLLLVHLKKGVLRGKVGP
RSEGEAA

Sequences:

>Translated_647_residues
MLYPVEFDVIVVGGGHAGTEAALAAARMGCQTLLLTHNIETLGQMSCNPSIGGIGKGHLVKEVDALGGAMALATDEGGIQ
FRILNSSKGPAVRATRAQADRVLYKAAIRHRLENQPNLMLFQQAVEDLLVEGDRVVGAVTQVGVRFRARAVVLTAGTFLD
GKIHVGLNNYTGGRAGDPAAVSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSVLEAQPGDLDPVPVFSFLGRTDMHPRQ
VPCWVTHTNERTHDIIRAGLNRSPMYTGVIEGVGPRYCPSIEDKIHRFASKDSHQIFLEPEGLTTNEFYPNGVSTSLPFD
VQLDLIHSMRGLEHAHILRPGYAIEYDYFDPRGLKASLESKAIGGLFFAGQINGTTGYEEAAAQGLLAGINAGLQVQGKA
AWTPRRDQAYLGVLVDDLITRGVTEPYRMFTSRAEFRLSLREDNADMRLTEAGRDLGVVDDARWDAFNRKRDAVSRETER
LKSTWVNPAILPAADAEPVLGKGIEREYSLADLLRRPNVTYESLMGMQGGKYAPDAPLADEPLLAEQIREQIEIGIKYHG
YIARQADEVERLGANENTRLPADFDYKQVRGLSIEVQQKLAQHKPETIGQASRISGITPAAVSLLLVHLKKGVLRGKVGP
RSEGEAA
>Mature_647_residues
MLYPVEFDVIVVGGGHAGTEAALAAARMGCQTLLLTHNIETLGQMSCNPSIGGIGKGHLVKEVDALGGAMALATDEGGIQ
FRILNSSKGPAVRATRAQADRVLYKAAIRHRLENQPNLMLFQQAVEDLLVEGDRVVGAVTQVGVRFRARAVVLTAGTFLD
GKIHVGLNNYTGGRAGDPAAVSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSVLEAQPGDLDPVPVFSFLGRTDMHPRQ
VPCWVTHTNERTHDIIRAGLNRSPMYTGVIEGVGPRYCPSIEDKIHRFASKDSHQIFLEPEGLTTNEFYPNGVSTSLPFD
VQLDLIHSMRGLEHAHILRPGYAIEYDYFDPRGLKASLESKAIGGLFFAGQINGTTGYEEAAAQGLLAGINAGLQVQGKA
AWTPRRDQAYLGVLVDDLITRGVTEPYRMFTSRAEFRLSLREDNADMRLTEAGRDLGVVDDARWDAFNRKRDAVSRETER
LKSTWVNPAILPAADAEPVLGKGIEREYSLADLLRRPNVTYESLMGMQGGKYAPDAPLADEPLLAEQIREQIEIGIKYHG
YIARQADEVERLGANENTRLPADFDYKQVRGLSIEVQQKLAQHKPETIGQASRISGITPAAVSLLLVHLKKGVLRGKVGP
RSEGEAA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=647, Percent_Identity=45.2859350850077, Blast_Score=491, Evalue=1e-139,
Organism=Homo sapiens, GI19882217, Length=673, Percent_Identity=43.8335809806835, Blast_Score=477, Evalue=1e-134,
Organism=Homo sapiens, GI183227703, Length=687, Percent_Identity=42.6491994177584, Blast_Score=473, Evalue=1e-133,
Organism=Escherichia coli, GI2367273, Length=636, Percent_Identity=70.5974842767296, Blast_Score=906, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=638, Percent_Identity=40.9090909090909, Blast_Score=460, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6321202, Length=631, Percent_Identity=45.8003169572108, Blast_Score=506, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24658174, Length=647, Percent_Identity=46.0587326120556, Blast_Score=503, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70553; Mature: 70553

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYPVEFDVIVVGGGHAGTEAALAAARMGCQTLLLTHNIETLGQMSCNPSIGGIGKGHLV
CCCEEEEEEEEEECCCCCHHHHHHHHHHCHHEEEEECCHHHHHCCCCCCCCCCCCCCHHH
KEVDALGGAMALATDEGGIQFRILNSSKGPAVRATRAQADRVLYKAAIRHRLENQPNLML
HHHHHCCCEEEEEECCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHH
FQQAVEDLLVEGDRVVGAVTQVGVRFRARAVVLTAGTFLDGKIHVGLNNYTGGRAGDPAA
HHHHHHHHHHCCCHHHHHHHHHCHHEEEEEEEEEECEEECCEEEEEECCCCCCCCCCCCE
VSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSVLEAQPGDLDPVPVFSFLGRTDMHPRQ
EEHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCCCCCCC
VPCWVTHTNERTHDIIRAGLNRSPMYTGVIEGVGPRYCPSIEDKIHRFASKDSHQIFLEP
CCEEEECCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEECC
EGLTTNEFYPNGVSTSLPFDVQLDLIHSMRGLEHAHILRPGYAIEYDYFDPRGLKASLES
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCHHEEECCCEEEEECCCCCCCCCHHHCC
KAIGGLFFAGQINGTTGYEEAAAQGLLAGINAGLQVQGKAAWTPRRDQAYLGVLVDDLIT
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
RGVTEPYRMFTSRAEFRLSLREDNADMRLTEAGRDLGVVDDARWDAFNRKRDAVSRETER
HCCCHHHHHHHCCCCEEEEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LKSTWVNPAILPAADAEPVLGKGIEREYSLADLLRRPNVTYESLMGMQGGKYAPDAPLAD
HHHHCCCCCEECCCCCCCHHCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCC
EPLLAEQIREQIEIGIKYHGYIARQADEVERLGANENTRLPADFDYKQVRGLSIEVQQKL
CCHHHHHHHHHHHCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHHHH
AQHKPETIGQASRISGITPAAVSLLLVHLKKGVLRGKVGPRSEGEAA
HHCCCHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLYPVEFDVIVVGGGHAGTEAALAAARMGCQTLLLTHNIETLGQMSCNPSIGGIGKGHLV
CCCEEEEEEEEEECCCCCHHHHHHHHHHCHHEEEEECCHHHHHCCCCCCCCCCCCCCHHH
KEVDALGGAMALATDEGGIQFRILNSSKGPAVRATRAQADRVLYKAAIRHRLENQPNLML
HHHHHCCCEEEEEECCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHH
FQQAVEDLLVEGDRVVGAVTQVGVRFRARAVVLTAGTFLDGKIHVGLNNYTGGRAGDPAA
HHHHHHHHHHCCCHHHHHHHHHCHHEEEEEEEEEECEEECCEEEEEECCCCCCCCCCCCE
VSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSVLEAQPGDLDPVPVFSFLGRTDMHPRQ
EEHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCCCCCCC
VPCWVTHTNERTHDIIRAGLNRSPMYTGVIEGVGPRYCPSIEDKIHRFASKDSHQIFLEP
CCEEEECCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEECC
EGLTTNEFYPNGVSTSLPFDVQLDLIHSMRGLEHAHILRPGYAIEYDYFDPRGLKASLES
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCHHEEECCCEEEEECCCCCCCCCHHHCC
KAIGGLFFAGQINGTTGYEEAAAQGLLAGINAGLQVQGKAAWTPRRDQAYLGVLVDDLIT
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
RGVTEPYRMFTSRAEFRLSLREDNADMRLTEAGRDLGVVDDARWDAFNRKRDAVSRETER
HCCCHHHHHHHCCCCEEEEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LKSTWVNPAILPAADAEPVLGKGIEREYSLADLLRRPNVTYESLMGMQGGKYAPDAPLAD
HHHHCCCCCEECCCCCCCHHCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCC
EPLLAEQIREQIEIGIKYHGYIARQADEVERLGANENTRLPADFDYKQVRGLSIEVQQKL
CCHHHHHHHHHHHCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHHHH
AQHKPETIGQASRISGITPAAVSLLLVHLKKGVLRGKVGPRSEGEAA
HHCCCHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA