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The map label for this gene is gidB

Identifier: 17548044

GI number: 17548044

Start: 3589219

End: 3589890

Strand: Reverse

Name: gidB

Synonym: RSc3327

Alternate gene names: 17548044

Gene position: 3589890-3589219 (Counterclockwise)

Preceding gene: 17548045

Following gene: 17548043

Centisome position: 96.6

GC content: 68.15

Gene sequence:

>672_bases
ATGGCGAACACCGCGCTGGCCGACACCCGGTCGGAACTGGAAGCCGGCGCGCGCGCGCTCGGCCTGCCGCTCGAGGGCGT
GCAACTGGATCGCCTGCTGGCGTACCAAGTGCTGCTCGCCAAGTGGAATCGCGTCTACAACCTGACCGCCATCCGTGATG
CCGGCGACATGCTGACGCATCATCTGCTGGATTCGCTGGCGGCCGTTCCGCGTATCGCGGAACTGGTGCGCCGCGTGCAG
CCCGAGGCGTCGCGTGTGCTGGATGTCGGTTCCGGCGGTGGCCTGCCCGGCATCCCGCTGGCGATCGCGTGCCCCGACAT
CGGCGTCACCATGGTCGACATCGTGCAGAAGAAGACCGCGTTCTTGACGCAATGCCGCGCAGAGCTGGGCCTGACGAATG
CCCAGTCGCACTGGGGCCACGTGGAAAAACTGGCCGATGCCGTCGGCTATGGCGTCATCACTTCGCGCGCATTTGCCGAG
CTGAACGACTTTGTGCGGTTGGCCGGCCATCTGCTGGCGCCGGGCGGGCGCATGGTCGCCATGAAAGGCGTGCGCCCGGA
CGCCGAAATTGCCCGGCTGCCCGAAGGGTGGGCGGTCGAGTCGATCGAGCGTTTGACGGTTCCGGGGCTGCCGGCCGAGC
GCCACCTGGTCATCCTCGCGCCGTCGGCGTGA

Upstream 100 bases:

>100_bases
CCGGCATCACGCCCGCGGCCGTGTCGCTGCTGCTGGTGCACCTGAAGAAGGGTGTGCTGCGTGGCAAGGTCGGCCCGCGT
TCCGAAGGCGAGGCCGCTTG

Downstream 100 bases:

>100_bases
GCGCACAAGCGAAGTCCGAGTCATTCACCGCAGAGGATTCATGTCGAATATTTTCGTGATCGCCAACCAGAAGGGCGGGG
TCGGCAAGACCACCACCACG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MANTALADTRSELEAGARALGLPLEGVQLDRLLAYQVLLAKWNRVYNLTAIRDAGDMLTHHLLDSLAAVPRIAELVRRVQ
PEASRVLDVGSGGGLPGIPLAIACPDIGVTMVDIVQKKTAFLTQCRAELGLTNAQSHWGHVEKLADAVGYGVITSRAFAE
LNDFVRLAGHLLAPGGRMVAMKGVRPDAEIARLPEGWAVESIERLTVPGLPAERHLVILAPSA

Sequences:

>Translated_223_residues
MANTALADTRSELEAGARALGLPLEGVQLDRLLAYQVLLAKWNRVYNLTAIRDAGDMLTHHLLDSLAAVPRIAELVRRVQ
PEASRVLDVGSGGGLPGIPLAIACPDIGVTMVDIVQKKTAFLTQCRAELGLTNAQSHWGHVEKLADAVGYGVITSRAFAE
LNDFVRLAGHLLAPGGRMVAMKGVRPDAEIARLPEGWAVESIERLTVPGLPAERHLVILAPSA
>Mature_222_residues
ANTALADTRSELEAGARALGLPLEGVQLDRLLAYQVLLAKWNRVYNLTAIRDAGDMLTHHLLDSLAAVPRIAELVRRVQP
EASRVLDVGSGGGLPGIPLAIACPDIGVTMVDIVQKKTAFLTQCRAELGLTNAQSHWGHVEKLADAVGYGVITSRAFAEL
NDFVRLAGHLLAPGGRMVAMKGVRPDAEIARLPEGWAVESIERLTVPGLPAERHLVILAPSA

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=199, Percent_Identity=42.2110552763819, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_RALSO (Q8XU66)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_521446.1
- ProteinModelPortal:   Q8XU66
- SMR:   Q8XU66
- GeneID:   1222191
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc3327
- NMPDR:   fig|267608.1.peg.3325
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- BioCyc:   RSOL267608:RSC3327-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23797; Mature: 23666

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTALADTRSELEAGARALGLPLEGVQLDRLLAYQVLLAKWNRVYNLTAIRDAGDMLTH
CCCCCHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHH
HLLDSLAAVPRIAELVRRVQPEASRVLDVGSGGGLPGIPLAIACPDIGVTMVDIVQKKTA
HHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
FLTQCRAELGLTNAQSHWGHVEKLADAVGYGVITSRAFAELNDFVRLAGHLLAPGGRMVA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
MKGVRPDAEIARLPEGWAVESIERLTVPGLPAERHLVILAPSA
EECCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
ANTALADTRSELEAGARALGLPLEGVQLDRLLAYQVLLAKWNRVYNLTAIRDAGDMLTH
CCCCHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHH
HLLDSLAAVPRIAELVRRVQPEASRVLDVGSGGGLPGIPLAIACPDIGVTMVDIVQKKTA
HHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
FLTQCRAELGLTNAQSHWGHVEKLADAVGYGVITSRAFAELNDFVRLAGHLLAPGGRMVA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
MKGVRPDAEIARLPEGWAVESIERLTVPGLPAERHLVILAPSA
EECCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852