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The map label for this gene is rep [H]

Identifier: 17548006

GI number: 17548006

Start: 3540395

End: 3542512

Strand: Reverse

Name: rep [H]

Synonym: RSc3289

Alternate gene names: 17548006

Gene position: 3542512-3540395 (Counterclockwise)

Preceding gene: 17548009

Following gene: 17548005

Centisome position: 95.32

GC content: 66.05

Gene sequence:

>2118_bases
ATGTCTTCCGGTCTTGCCCACGGGCTCAATGCTGCCCAATCCGAAGGTGTGCACTACCTCGATGGTCCGTGCCTGGTGCT
GGCGGGCGCGGGCTCCGGCAAGACCCGGGTGATCACGCAGAAGATCGCGCACCTGATCCTGGACAAGGGCTTCGAGCCGC
GCCACATCGCCGCCGTCACCTTCACCAACAAGGCGGCCAAGGAAATGCAGGAGCGCGTGGCCAAGCTGATGGACGGCAAG
ACGCGCGAGGACGGCAAGCGCATCCCCATCAAGCAGCTGACGGTGTGTACCTTCCACTCGCTGGGCGTGCAGATCCTGCG
CGCGGAGGCCGAGCACGTGGGCCTGAAGCCGCGCTTCTCGATCATGGATTCGGACGATTGCTTCGGCCTGATCCAGGAGC
AGCTGGCGACGACGGACAAGCAGCTGATCCGCCGCGTGCAGAGCGCGATCTCGCTGTGGAAGAACGGTCTCGTCGATCCG
GAGGCCGCCATCGCCGAGGCCATCGCCAACAACAACGCCGACGACCACCAGGCCGCGCTGGTCTACCGCAACTACGTCGC
CACGCTGCATGCCTACCAGGCGGTGGATTTCGACGACCTGATCCGCATCCCGGCCGAGCTGTTCGCGCGCAACGAAGACG
TGCGCCTGAAGTGGCAGAACCGCCTGCGCTACTTCCTCGTCGACGAATACCAGGACACCAACGCCTGCCAGTACCAGCTG
CTCAAGCTGCTGGCCGGCGGCTCGCACCTGCGCGCGCCGGCCTTCACGGCGGTGGGCGACGACGACCAGGCGATCTACGG
CTGGCGCGGCGCGACGCTGGACAACCTCAAGCTGCTGCAGACCGATTTCCCCAGCCTGAAGGTCATCAAGCTGGAACAGA
ACTACCGCTCCACCGTGCGCATCCTGAACGCCGCCAACGCGGTGATCGCGCAGAACCCCAAGCTGTTCGAAAAAACGCTG
TGGAGCGAGCACGGCATGGGCGATGCGATCAACGTGTCGGGCGCCAACGATGAGGAGCACGAGGCCGAGAGCGTGGTGTT
CAAGCTCTCCGCGCACAAGTTCGAGCGGCGCGCGCAGTTCCGCGACTACGCCATCCTGTACCGCGGCAATTTCCAGGCGC
GGCTGTTCGAGCAGATCCTGCGGCGCGAGCGCATTCCGTATGTGCTCTCGGGCGGCCAGAGCTTCTTCGACAAGGCCGAG
ATCAAGGACCTGTGCGCCTACCTGCGCCTGATCGCCAACGCCGACGACGATCCCGCCTTCATCCGCGCCGTCACCACGCC
CAAGCGCGGCGTGGGCAACGCGACGCTGGAGGTGCTCGGCGCGTTCGCGGGGCAGGCCAAGGTATCGCTGTTCGAGGCGG
CGATGATGGGCGGCATCGAGGCCAAGCTGGCGCCGCGCCAGCTGGAGCCGCTGCGGGTGTTCTGCGAATTCATCGTCCGC
CTGTCGGACCGCGCCGCGCGCGAGCCGGCCAGCACGCTGCTCGACGAGATGATGGGCGGCATCCACTACGAGGCCTACCT
GTACGACACCTACGACGAGCGCCAGGCCCAGAACAAGTGGACCAACGTGCTGGAGTTCCTCGACTGGCTCAAGCGCAAGG
GCACCAAGCCGGAGAACCTCGAAGCCGAGGAGGCCACCGGCTACGACAACGCCGACGGCCTGATGGATACCGGCAAGAAC
CTGCTGGAGCTCACGCAGACCATCGCGCTCATCAGCATGCTCGAAGGCAAGGAGGAGGACCCGGACGCGGTGCGCCTGTC
CACGCTGCATGCGAGCAAGGGCCTAGAGTATCCGCACGTGTTCCTGGTGGGCGTGGAAGAGGGCATCCTGCCCCACTGCC
GCGAAGACGACGACCTGACCGACGAGAAGATCGAGGAAGAGCGCCGCCTCATGTACGTCGGCATCACCCGCGCCCAGCGC
AGCCTGCATATCACCTGGTGCAAGAGGCGCAAGCGCGCGCGAGAGACCGTGGTGTGCGAACCGTCGCGCTTCATCGCCGA
GATGAAGCTGGGCGACGACGCCGGCCCGACCCCGGAAGACACCATGCCCGCCATGTCGCCGAAGGACCGCCTCGGGGCAC
TCAAGGGCCTGCTGGCCGCAAAGAAGACCGCGGCGTAA

Upstream 100 bases:

>100_bases
GCGCGGGGCGGCGAAATACGGGATCGACCACCGGGGACTGGCCCCTGATCGCGTGCTACCATCCCACCCCGCCTCACCGT
CATCCCGCCTGCTGATCCCC

Downstream 100 bases:

>100_bases
CCCGCGCAAGCCGCGCAAGCCGCGCCCGGCAAGGGTGCGCCGCCCCGGCCCGAGCGCTCCCGGGCCGCATCCCCGCGCAC
GCGCATCACAACGTTCCAAG

Product: ATP-dependent DNA helicase REP protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MSSGLAHGLNAAQSEGVHYLDGPCLVLAGAGSGKTRVITQKIAHLILDKGFEPRHIAAVTFTNKAAKEMQERVAKLMDGK
TREDGKRIPIKQLTVCTFHSLGVQILRAEAEHVGLKPRFSIMDSDDCFGLIQEQLATTDKQLIRRVQSAISLWKNGLVDP
EAAIAEAIANNNADDHQAALVYRNYVATLHAYQAVDFDDLIRIPAELFARNEDVRLKWQNRLRYFLVDEYQDTNACQYQL
LKLLAGGSHLRAPAFTAVGDDDQAIYGWRGATLDNLKLLQTDFPSLKVIKLEQNYRSTVRILNAANAVIAQNPKLFEKTL
WSEHGMGDAINVSGANDEEHEAESVVFKLSAHKFERRAQFRDYAILYRGNFQARLFEQILRRERIPYVLSGGQSFFDKAE
IKDLCAYLRLIANADDDPAFIRAVTTPKRGVGNATLEVLGAFAGQAKVSLFEAAMMGGIEAKLAPRQLEPLRVFCEFIVR
LSDRAAREPASTLLDEMMGGIHYEAYLYDTYDERQAQNKWTNVLEFLDWLKRKGTKPENLEAEEATGYDNADGLMDTGKN
LLELTQTIALISMLEGKEEDPDAVRLSTLHASKGLEYPHVFLVGVEEGILPHCREDDDLTDEKIEEERRLMYVGITRAQR
SLHITWCKRRKRARETVVCEPSRFIAEMKLGDDAGPTPEDTMPAMSPKDRLGALKGLLAAKKTAA

Sequences:

>Translated_705_residues
MSSGLAHGLNAAQSEGVHYLDGPCLVLAGAGSGKTRVITQKIAHLILDKGFEPRHIAAVTFTNKAAKEMQERVAKLMDGK
TREDGKRIPIKQLTVCTFHSLGVQILRAEAEHVGLKPRFSIMDSDDCFGLIQEQLATTDKQLIRRVQSAISLWKNGLVDP
EAAIAEAIANNNADDHQAALVYRNYVATLHAYQAVDFDDLIRIPAELFARNEDVRLKWQNRLRYFLVDEYQDTNACQYQL
LKLLAGGSHLRAPAFTAVGDDDQAIYGWRGATLDNLKLLQTDFPSLKVIKLEQNYRSTVRILNAANAVIAQNPKLFEKTL
WSEHGMGDAINVSGANDEEHEAESVVFKLSAHKFERRAQFRDYAILYRGNFQARLFEQILRRERIPYVLSGGQSFFDKAE
IKDLCAYLRLIANADDDPAFIRAVTTPKRGVGNATLEVLGAFAGQAKVSLFEAAMMGGIEAKLAPRQLEPLRVFCEFIVR
LSDRAAREPASTLLDEMMGGIHYEAYLYDTYDERQAQNKWTNVLEFLDWLKRKGTKPENLEAEEATGYDNADGLMDTGKN
LLELTQTIALISMLEGKEEDPDAVRLSTLHASKGLEYPHVFLVGVEEGILPHCREDDDLTDEKIEEERRLMYVGITRAQR
SLHITWCKRRKRARETVVCEPSRFIAEMKLGDDAGPTPEDTMPAMSPKDRLGALKGLLAAKKTAA
>Mature_704_residues
SSGLAHGLNAAQSEGVHYLDGPCLVLAGAGSGKTRVITQKIAHLILDKGFEPRHIAAVTFTNKAAKEMQERVAKLMDGKT
REDGKRIPIKQLTVCTFHSLGVQILRAEAEHVGLKPRFSIMDSDDCFGLIQEQLATTDKQLIRRVQSAISLWKNGLVDPE
AAIAEAIANNNADDHQAALVYRNYVATLHAYQAVDFDDLIRIPAELFARNEDVRLKWQNRLRYFLVDEYQDTNACQYQLL
KLLAGGSHLRAPAFTAVGDDDQAIYGWRGATLDNLKLLQTDFPSLKVIKLEQNYRSTVRILNAANAVIAQNPKLFEKTLW
SEHGMGDAINVSGANDEEHEAESVVFKLSAHKFERRAQFRDYAILYRGNFQARLFEQILRRERIPYVLSGGQSFFDKAEI
KDLCAYLRLIANADDDPAFIRAVTTPKRGVGNATLEVLGAFAGQAKVSLFEAAMMGGIEAKLAPRQLEPLRVFCEFIVRL
SDRAAREPASTLLDEMMGGIHYEAYLYDTYDERQAQNKWTNVLEFLDWLKRKGTKPENLEAEEATGYDNADGLMDTGKNL
LELTQTIALISMLEGKEEDPDAVRLSTLHASKGLEYPHVFLVGVEEGILPHCREDDDLTDEKIEEERRLMYVGITRAQRS
LHITWCKRRKRARETVVCEPSRFIAEMKLGDDAGPTPEDTMPAMSPKDRLGALKGLLAAKKTAA

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=704, Percent_Identity=47.4431818181818, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI2367296, Length=677, Percent_Identity=35.8936484490399, Blast_Score=349, Evalue=4e-97,
Organism=Saccharomyces cerevisiae, GI6322369, Length=724, Percent_Identity=28.4530386740331, Blast_Score=201, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6324477, Length=674, Percent_Identity=23.1454005934718, Blast_Score=103, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78897; Mature: 78766

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGLAHGLNAAQSEGVHYLDGPCLVLAGAGSGKTRVITQKIAHLILDKGFEPRHIAAVT
CCCCCHHCCCHHHHCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
FTNKAAKEMQERVAKLMDGKTREDGKRIPIKQLTVCTFHSLGVQILRAEAEHVGLKPRFS
ECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IMDSDDCFGLIQEQLATTDKQLIRRVQSAISLWKNGLVDPEAAIAEAIANNNADDHQAAL
ECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHH
VYRNYVATLHAYQAVDFDDLIRIPAELFARNEDVRLKWQNRLRYFLVDEYQDTNACQYQL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCHHHHHH
LKLLAGGSHLRAPAFTAVGDDDQAIYGWRGATLDNLKLLQTDFPSLKVIKLEQNYRSTVR
HHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEEEECCHHHHHHH
ILNAANAVIAQNPKLFEKTLWSEHGMGDAINVSGANDEEHEAESVVFKLSAHKFERRAQF
HHHHCCHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RDYAILYRGNFQARLFEQILRRERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAF
CCEEEEEECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IRAVTTPKRGVGNATLEVLGAFAGQAKVSLFEAAMMGGIEAKLAPRQLEPLRVFCEFIVR
EEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHH
LSDRAAREPASTLLDEMMGGIHYEAYLYDTYDERQAQNKWTNVLEFLDWLKRKGTKPENL
HHHHHHCCHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EAEEATGYDNADGLMDTGKNLLELTQTIALISMLEGKEEDPDAVRLSTLHASKGLEYPHV
CCHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCCCCEE
FLVGVEEGILPHCREDDDLTDEKIEEERRLMYVGITRAQRSLHITWCKRRKRARETVVCE
EEEECCCCCCCCCCCCCCCCHHHHHHHHCEEEEEEECCCCCEEHHHHHHHHHHCCCEEEC
PSRFIAEMKLGDDAGPTPEDTMPAMSPKDRLGALKGLLAAKKTAA
CHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSGLAHGLNAAQSEGVHYLDGPCLVLAGAGSGKTRVITQKIAHLILDKGFEPRHIAAVT
CCCCHHCCCHHHHCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
FTNKAAKEMQERVAKLMDGKTREDGKRIPIKQLTVCTFHSLGVQILRAEAEHVGLKPRFS
ECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IMDSDDCFGLIQEQLATTDKQLIRRVQSAISLWKNGLVDPEAAIAEAIANNNADDHQAAL
ECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHH
VYRNYVATLHAYQAVDFDDLIRIPAELFARNEDVRLKWQNRLRYFLVDEYQDTNACQYQL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCHHHHHH
LKLLAGGSHLRAPAFTAVGDDDQAIYGWRGATLDNLKLLQTDFPSLKVIKLEQNYRSTVR
HHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEEEECCHHHHHHH
ILNAANAVIAQNPKLFEKTLWSEHGMGDAINVSGANDEEHEAESVVFKLSAHKFERRAQF
HHHHCCHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RDYAILYRGNFQARLFEQILRRERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAF
CCEEEEEECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IRAVTTPKRGVGNATLEVLGAFAGQAKVSLFEAAMMGGIEAKLAPRQLEPLRVFCEFIVR
EEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHH
LSDRAAREPASTLLDEMMGGIHYEAYLYDTYDERQAQNKWTNVLEFLDWLKRKGTKPENL
HHHHHHCCHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EAEEATGYDNADGLMDTGKNLLELTQTIALISMLEGKEEDPDAVRLSTLHASKGLEYPHV
CCHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCCCCEE
FLVGVEEGILPHCREDDDLTDEKIEEERRLMYVGITRAQRSLHITWCKRRKRARETVVCE
EEEECCCCCCCCCCCCCCCCHHHHHHHHCEEEEEEECCCCCEEHHHHHHHHHHCCCEEEC
PSRFIAEMKLGDDAGPTPEDTMPAMSPKDRLGALKGLLAAKKTAA
CHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]