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The map label for this gene is gcl [H]

Identifier: 17547998

GI number: 17547998

Start: 3530896

End: 3532671

Strand: Direct

Name: gcl [H]

Synonym: RSc3281

Alternate gene names: 17547998

Gene position: 3530896-3532671 (Clockwise)

Preceding gene: 17547994

Following gene: 17547999

Centisome position: 95.01

GC content: 69.09

Gene sequence:

>1776_bases
ATGCCGAAGATGAGAGCGATCGACGCCGCCGTTGCGGTGCTGGAGCAGGAAGGCATTACCACGGCCTTCGGCGTGCCCGG
CGCCGCCATCAACCCGCTGTATTCGGCGCTGCGCCGCGCCGGCAGCATCGACCACGTGCTGGCCCGCCATGTCGAGGGCG
CCTCGCACATGGCCGAGGGCTACACCCGTGCCGAGCCGGGCAACATCGGCGTGTGCATCGGCACCTCGGGGCCGGCCGGC
ACCGACATGATCACCGGCCTGTATTCGGCCTGGGCGGACTCGATTCCCATCCTCTGCATCACCGGCCAGGCGCCGCGCGC
ACGGCTGTACAAGGAAGACTTCCAGGCCGTCGACATCGAGTCGATCGCCAAGCCGGTCACCAAGTGGGCCGTGACGGTGC
GCGAGCCGGCGTTGGTGCCGCGCGTGTTCCAGCAGGCCTTCCACCTGATGCGCTCGGGCCGGCCGGGGCCGGTGCTGATC
GACCTGCCGTTCGACGTGCAGGTCGCCGAGATCGAGTTCGACCCCGACACCTACGCCCCGCTGCCCGTCTACAAGCCCGC
CGCCACGCGCGCCCAGGCCGAACGTGCCATCGCGATGCTGTGCGCGGCCGAGCGCCCGCTGCTGGTGTGCGGCGGCGGCG
TCATCAACGCCGACGCGGCCCGGCTGATGGCCGAGTTCGCCGAGCTGGTGAACGTGCCGGTGGTGCCCACGCTGATGGGC
TGGGGCGTGCTCGCCGACGATCACCCGCTCAACGCCGGCATGGTCGGCCTGCAGACCTCGCACCGCTACGGCAACGCCAA
CCTGCTCGCCTCGGACTTCGTGTTCGGCATCGGCAACCGCTGGGCCAACCGCCATACCGGCAGCATCGACGTCTACACGA
AGGGCCGCACGTTCATCCACGTGGATATCGAGCCCACGCAGATCGGCCGCGTGTTCGGCCCGGACCTGGGCATCGTGTCG
GATGCGAAGGCCGCGCTGGAGCGGTTCATTGACGTGGCGCGCGAGTGGAAGGCCGCCGGCAAGCTGCCGTGCCGCCGGGC
CTGGAACGCCGACGTGCAGAAGCGCAAGCGCACCCTGCTGCGCCGCTCGGATTTCGACGACGTGCCGATCAAGCCGCAGC
GCGTGTACCGCGAGATGAACCGGTACTTCCCGCGCGACGTCCGCTACGTGAGCACGATCGGCCTGTCGCAGATCGCCGCC
GCGCAGTTCCTGTCGGTCAACCAGCCGCGCCACTGGATCAACTGCGGCCAGGCCGGCCCGCTGGGCTGGACGATCCCCGC
CGCGATCGGCGTGAAGGTGGCCTCGCCCGGCAGCGACGTGGTGGCCATCTCGGGCGACTACGACTTCCAGTTCATGATCG
AAGAGCTGGCCGTGGCCGCGCAGTTCAAGGTGCCCTACATCCACGTGGTGGTGAACAACGCCTACCTGGGCCTGATCCGC
CAGGCGCAGCGCAACTTCGACATGGACTACTGCGTGCAGCTCGCCTTCGACAACGTCAACGCGCCCGGGCTCGACGGCTA
CGGCGTGGACCACGTCAAGGTGGTCGAGGGGCTGGGTTGCAAGGCCCTGCGCGTATTCAGGCCGCAGGACATCGCGCCCG
CCTTCGCGCAGGCCCGCCTGCTGATGGCCGAGCACTCGGTGCCGGTGGTGGTGGAGGTGATCCTGGAGCGCGTGACCAAC
ATCGCCATGGGCACCGAGATCGACAACGTGGTCGAGTTCGACACGCCCCTCGACCTGCAAGACACGCTGGACGAAGCCGA
AGGCGCCACCACCTGA

Upstream 100 bases:

>100_bases
TTCGCAATCAACTAACCAAAGCTGCGTTTATCCGTATCGGCATGCGGGAATAGGATCAGTTCCAACCGATTCCCACCCAT
CCCCCTCCCGGAGACACACC

Downstream 100 bases:

>100_bases
GCACACGCACCACAGACACGGAGACCGACATGCCGAAGCTGGCTGCCAACCTGACCATGCTGTTCAACGAACACGCCTTC
CTCGAGCGCTTCGAAGCCGC

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MPKMRAIDAAVAVLEQEGITTAFGVPGAAINPLYSALRRAGSIDHVLARHVEGASHMAEGYTRAEPGNIGVCIGTSGPAG
TDMITGLYSAWADSIPILCITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLI
DLPFDVQVAEIEFDPDTYAPLPVYKPAATRAQAERAIAMLCAAERPLLVCGGGVINADAARLMAEFAELVNVPVVPTLMG
WGVLADDHPLNAGMVGLQTSHRYGNANLLASDFVFGIGNRWANRHTGSIDVYTKGRTFIHVDIEPTQIGRVFGPDLGIVS
DAKAALERFIDVAREWKAAGKLPCRRAWNADVQKRKRTLLRRSDFDDVPIKPQRVYREMNRYFPRDVRYVSTIGLSQIAA
AQFLSVNQPRHWINCGQAGPLGWTIPAAIGVKVASPGSDVVAISGDYDFQFMIEELAVAAQFKVPYIHVVVNNAYLGLIR
QAQRNFDMDYCVQLAFDNVNAPGLDGYGVDHVKVVEGLGCKALRVFRPQDIAPAFAQARLLMAEHSVPVVVEVILERVTN
IAMGTEIDNVVEFDTPLDLQDTLDEAEGATT

Sequences:

>Translated_591_residues
MPKMRAIDAAVAVLEQEGITTAFGVPGAAINPLYSALRRAGSIDHVLARHVEGASHMAEGYTRAEPGNIGVCIGTSGPAG
TDMITGLYSAWADSIPILCITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLI
DLPFDVQVAEIEFDPDTYAPLPVYKPAATRAQAERAIAMLCAAERPLLVCGGGVINADAARLMAEFAELVNVPVVPTLMG
WGVLADDHPLNAGMVGLQTSHRYGNANLLASDFVFGIGNRWANRHTGSIDVYTKGRTFIHVDIEPTQIGRVFGPDLGIVS
DAKAALERFIDVAREWKAAGKLPCRRAWNADVQKRKRTLLRRSDFDDVPIKPQRVYREMNRYFPRDVRYVSTIGLSQIAA
AQFLSVNQPRHWINCGQAGPLGWTIPAAIGVKVASPGSDVVAISGDYDFQFMIEELAVAAQFKVPYIHVVVNNAYLGLIR
QAQRNFDMDYCVQLAFDNVNAPGLDGYGVDHVKVVEGLGCKALRVFRPQDIAPAFAQARLLMAEHSVPVVVEVILERVTN
IAMGTEIDNVVEFDTPLDLQDTLDEAEGATT
>Mature_590_residues
PKMRAIDAAVAVLEQEGITTAFGVPGAAINPLYSALRRAGSIDHVLARHVEGASHMAEGYTRAEPGNIGVCIGTSGPAGT
DMITGLYSAWADSIPILCITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLID
LPFDVQVAEIEFDPDTYAPLPVYKPAATRAQAERAIAMLCAAERPLLVCGGGVINADAARLMAEFAELVNVPVVPTLMGW
GVLADDHPLNAGMVGLQTSHRYGNANLLASDFVFGIGNRWANRHTGSIDVYTKGRTFIHVDIEPTQIGRVFGPDLGIVSD
AKAALERFIDVAREWKAAGKLPCRRAWNADVQKRKRTLLRRSDFDDVPIKPQRVYREMNRYFPRDVRYVSTIGLSQIAAA
QFLSVNQPRHWINCGQAGPLGWTIPAAIGVKVASPGSDVVAISGDYDFQFMIEELAVAAQFKVPYIHVVVNNAYLGLIRQ
AQRNFDMDYCVQLAFDNVNAPGLDGYGVDHVKVVEGLGCKALRVFRPQDIAPAFAQARLLMAEHSVPVVVEVILERVTNI
AMGTEIDNVVEFDTPLDLQDTLDEAEGATT

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=24.1071428571429, Blast_Score=135, Evalue=2e-31,
Organism=Homo sapiens, GI21361361, Length=564, Percent_Identity=23.758865248227, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1786717, Length=590, Percent_Identity=75.7627118644068, Blast_Score=941, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=549, Percent_Identity=33.5154826958106, Blast_Score=322, Evalue=5e-89,
Organism=Escherichia coli, GI1790104, Length=476, Percent_Identity=34.453781512605, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI1787096, Length=548, Percent_Identity=23.9051094890511, Blast_Score=155, Evalue=1e-38,
Organism=Escherichia coli, GI1788716, Length=481, Percent_Identity=27.027027027027, Blast_Score=141, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=507, Percent_Identity=23.4714003944773, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=507, Percent_Identity=23.4714003944773, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17542570, Length=556, Percent_Identity=24.8201438848921, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=601, Percent_Identity=30.9484193011647, Blast_Score=272, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6320816, Length=567, Percent_Identity=24.5149911816578, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19922626, Length=488, Percent_Identity=26.0245901639344, Blast_Score=146, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64361; Mature: 64229

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKMRAIDAAVAVLEQEGITTAFGVPGAAINPLYSALRRAGSIDHVLARHVEGASHMAEG
CCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCC
YTRAEPGNIGVCIGTSGPAGTDMITGLYSAWADSIPILCITGQAPRARLYKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPFDVQVAEIEFDPDTYAP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAATRAQAERAIAMLCAAERPLLVCGGGVINADAARLMAEFAELVNVPVVPTLMG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEECHHHHHHHHHHHHHHCCCCHHHHHC
WGVLADDHPLNAGMVGLQTSHRYGNANLLASDFVFGIGNRWANRHTGSIDVYTKGRTFIH
CCEECCCCCCCCCEEEEECCCCCCCCCEEHHHHHHHCCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALERFIDVAREWKAAGKLPCRRAWNADVQKRKRTLL
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
RRSDFDDVPIKPQRVYREMNRYFPRDVRYVSTIGLSQIAAAQFLSVNQPRHWINCGQAGP
HHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCHHCCCCCCCC
LGWTIPAAIGVKVASPGSDVVAISGDYDFQFMIEELAVAAQFKVPYIHVVVNNAYLGLIR
CCEECHHHHEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHH
QAQRNFDMDYCVQLAFDNVNAPGLDGYGVDHVKVVEGLGCKALRVFRPQDIAPAFAQARL
HHHHCCCHHHHHEEEECCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
LMAEHSVPVVVEVILERVTNIAMGTEIDNVVEFDTPLDLQDTLDEAEGATT
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure 
PKMRAIDAAVAVLEQEGITTAFGVPGAAINPLYSALRRAGSIDHVLARHVEGASHMAEG
CCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCC
YTRAEPGNIGVCIGTSGPAGTDMITGLYSAWADSIPILCITGQAPRARLYKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPFDVQVAEIEFDPDTYAP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAATRAQAERAIAMLCAAERPLLVCGGGVINADAARLMAEFAELVNVPVVPTLMG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEECHHHHHHHHHHHHHHCCCCHHHHHC
WGVLADDHPLNAGMVGLQTSHRYGNANLLASDFVFGIGNRWANRHTGSIDVYTKGRTFIH
CCEECCCCCCCCCEEEEECCCCCCCCCEEHHHHHHHCCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALERFIDVAREWKAAGKLPCRRAWNADVQKRKRTLL
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
RRSDFDDVPIKPQRVYREMNRYFPRDVRYVSTIGLSQIAAAQFLSVNQPRHWINCGQAGP
HHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCHHCCCCCCCC
LGWTIPAAIGVKVASPGSDVVAISGDYDFQFMIEELAVAAQFKVPYIHVVVNNAYLGLIR
CCEECHHHHEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHH
QAQRNFDMDYCVQLAFDNVNAPGLDGYGVDHVKVVEGLGCKALRVFRPQDIAPAFAQARL
HHHHCCCHHHHHEEEECCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
LMAEHSVPVVVEVILERVTNIAMGTEIDNVVEFDTPLDLQDTLDEAEGATT
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]