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The map label for this gene is dmlR [H]

Identifier: 17547997

GI number: 17547997

Start: 3529637

End: 3530548

Strand: Reverse

Name: dmlR [H]

Synonym: RSc3280

Alternate gene names: 17547997

Gene position: 3530548-3529637 (Counterclockwise)

Preceding gene: 17548001

Following gene: 17547996

Centisome position: 95.0

GC content: 69.08

Gene sequence:

>912_bases
ATGGACAAGCTCAAGCAGATCGAGGCCTTCATCGCCGTGGTGGAGCACGGCAGCATGGCGGCCGCCGCGCTGACGCAGGA
CGTGACCCCGGTGATGATCGGCCGGCGCATCAACGCGCTGGAGGCACGCCTGGGCGTCAGGCTGCTGCACCGCTCGACGC
GCCGCATCGCCGTGACGGAGCAGGGCGCGGTGTTCATGGAGCAGTGCAAGAAGGCCCTGTCTGAACTGGACCGTGCCGAA
CTGCTGGTCGCCGAAGGGCGGCACAAGGCGACAGGGCACCTGATCGTGTCCGCGCCGGCCGCGTTCGGGCGCAAGCACGT
GGCGCCGCACGCGCCCGCTTTCCTGCGCGCGAACCCGGACGTGCGCCTGTCGTTCAACCTGACCGACCGCGTGGTCGACC
TGGTGCGCGAGGGCTACGACCTCGGCATCCGCATCGGCGGGGCGATCGATCCGAACTTCGTGGCGATCCGCCTGGCGTCG
AACCAGCGCGTGGTGTGCGGCACGCCCGACTACTTCGCCCGGCACGGCGTGCCGCGCACCCTCGACGACCTGTCGCGCCA
CAACTGCCTGGCCTTCAACCTGCAGGGCGGCCAGCAGCGCGGCTGGTATTTCCAGCACAACGGCAGGATGGTCACCGTCC
GGGTCAGCGGCAATCTCGACTGCAACGACGGCGAACTGCTGCACCGCTGGATGGGCGAGGGCCTGGGCCTGGGCTGGCGC
TCGACCTGGGAGATCCAGCCCGAGCTGGAATCCGGCGCGCTGATGACGGTGCTGGACGACTACGCGCTGCCCGGCTACGA
CATCCTCGCCGTCTATCCGCAGCAACGGCCGGTGCCGGCCAAGATCCGCTTCTTCATCGAGCACCTGAAGGCGATCTACG
CGCAGCCGGGGTACTGGTCGCGCGCAGATTGA

Upstream 100 bases:

>100_bases
CATGTCCGCCATGCCGCCGGCGTGATACCGGGCCGGAAACCATCATTCGGGTACCGAATCGTTCCTACAATGTCCGGACA
TTGCAGACTCGGACGATGGA

Downstream 100 bases:

>100_bases
GGCGATGTCCGATCGACATGGCGCGCCGTGCCGGGAACGATGGCTGACCGGAAAGCAGCGGTCATCGCCATCGCATGAAA
ACTTATATCATAACGCATAA

Product: transcription regulator protein

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MDKLKQIEAFIAVVEHGSMAAAALTQDVTPVMIGRRINALEARLGVRLLHRSTRRIAVTEQGAVFMEQCKKALSELDRAE
LLVAEGRHKATGHLIVSAPAAFGRKHVAPHAPAFLRANPDVRLSFNLTDRVVDLVREGYDLGIRIGGAIDPNFVAIRLAS
NQRVVCGTPDYFARHGVPRTLDDLSRHNCLAFNLQGGQQRGWYFQHNGRMVTVRVSGNLDCNDGELLHRWMGEGLGLGWR
STWEIQPELESGALMTVLDDYALPGYDILAVYPQQRPVPAKIRFFIEHLKAIYAQPGYWSRAD

Sequences:

>Translated_303_residues
MDKLKQIEAFIAVVEHGSMAAAALTQDVTPVMIGRRINALEARLGVRLLHRSTRRIAVTEQGAVFMEQCKKALSELDRAE
LLVAEGRHKATGHLIVSAPAAFGRKHVAPHAPAFLRANPDVRLSFNLTDRVVDLVREGYDLGIRIGGAIDPNFVAIRLAS
NQRVVCGTPDYFARHGVPRTLDDLSRHNCLAFNLQGGQQRGWYFQHNGRMVTVRVSGNLDCNDGELLHRWMGEGLGLGWR
STWEIQPELESGALMTVLDDYALPGYDILAVYPQQRPVPAKIRFFIEHLKAIYAQPGYWSRAD
>Mature_303_residues
MDKLKQIEAFIAVVEHGSMAAAALTQDVTPVMIGRRINALEARLGVRLLHRSTRRIAVTEQGAVFMEQCKKALSELDRAE
LLVAEGRHKATGHLIVSAPAAFGRKHVAPHAPAFLRANPDVRLSFNLTDRVVDLVREGYDLGIRIGGAIDPNFVAIRLAS
NQRVVCGTPDYFARHGVPRTLDDLSRHNCLAFNLQGGQQRGWYFQHNGRMVTVRVSGNLDCNDGELLHRWMGEGLGLGWR
STWEIQPELESGALMTVLDDYALPGYDILAVYPQQRPVPAKIRFFIEHLKAIYAQPGYWSRAD

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=35.6164383561644, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1789440, Length=287, Percent_Identity=34.8432055749129, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=30.1694915254237, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787128, Length=294, Percent_Identity=29.2517006802721, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=27.3037542662116, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=27.7027027027027, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789639, Length=299, Percent_Identity=27.0903010033445, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786448, Length=200, Percent_Identity=27, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=24.3150684931507, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790011, Length=293, Percent_Identity=19.1126279863481, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=259, Percent_Identity=23.5521235521236, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33768; Mature: 33768

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLKQIEAFIAVVEHGSMAAAALTQDVTPVMIGRRINALEARLGVRLLHRSTRRIAVTE
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEC
QGAVFMEQCKKALSELDRAELLVAEGRHKATGHLIVSAPAAFGRKHVAPHAPAFLRANPD
CCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCEEEEECCHHHCCCCCCCCCCEEEECCCC
VRLSFNLTDRVVDLVREGYDLGIRIGGAIDPNFVAIRLASNQRVVCGTPDYFARHGVPRT
EEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCCCHHHHCCCCCC
LDDLSRHNCLAFNLQGGQQRGWYFQHNGRMVTVRVSGNLDCNDGELLHRWMGEGLGLGWR
HHHHCCCCEEEEEECCCCCCCEEEEECCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCC
STWEIQPELESGALMTVLDDYALPGYDILAVYPQQRPVPAKIRFFIEHLKAIYAQPGYWS
CEEEECCCCCCCEEEEEEHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
RAD
CCC
>Mature Secondary Structure
MDKLKQIEAFIAVVEHGSMAAAALTQDVTPVMIGRRINALEARLGVRLLHRSTRRIAVTE
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEC
QGAVFMEQCKKALSELDRAELLVAEGRHKATGHLIVSAPAAFGRKHVAPHAPAFLRANPD
CCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCEEEEECCHHHCCCCCCCCCCEEEECCCC
VRLSFNLTDRVVDLVREGYDLGIRIGGAIDPNFVAIRLASNQRVVCGTPDYFARHGVPRT
EEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCCCHHHHCCCCCC
LDDLSRHNCLAFNLQGGQQRGWYFQHNGRMVTVRVSGNLDCNDGELLHRWMGEGLGLGWR
HHHHCCCCEEEEEECCCCCCCEEEEECCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCC
STWEIQPELESGALMTVLDDYALPGYDILAVYPQQRPVPAKIRFFIEHLKAIYAQPGYWS
CEEEECCCCCCCEEEEEEHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
RAD
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]