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The map label for this gene is gspF [H]

Identifier: 17547835

GI number: 17547835

Start: 3354195

End: 3355406

Strand: Direct

Name: gspF [H]

Synonym: RSc3116

Alternate gene names: 17547835

Gene position: 3354195-3355406 (Clockwise)

Preceding gene: 17547834

Following gene: 17547836

Centisome position: 90.25

GC content: 69.8

Gene sequence:

>1212_bases
ATGCCAGCCTTCCGCTACGAAGCCGCCGACGCCTCCGGCCGCACCGACCGCGGCGTCATCGAAGCCGACAGCGCCCGCCA
GGCGCGCACCGCGCTGCGCGCCCGCGGGCTGACGCCGCTGGTGGTCGATGCGCTCGGTGCGCAGGCCGCCAAGCGCGCGG
GCTCCCGTTTCGGCAAGCGGCTCTCGCCGCAGGAAAACGCCCTGGTGACGCGCCAGCTCGCCAGCCTGCTGGTCGCCGGC
CTGCCGCTGGACGAAGCGCTGGCCGCGCTGGCCGACCAGGCCGAACGCCCCTATGTGCACGAGCTGCTGGCATCCATCCG
CGCCGAGGTGGTGGGCGGCAGCTCGCTGTCGGTGGCGCTGGCCCAGCATCCGCGCGATTTTCCGGACATCTACCGCGCGC
TGGTCTCGGCCGGCGAGCATTCGGGCAACCTGGGGCTGGTGCTGTCGCGCCTGGCCGACTACATCGAAAGCCGCAACGCG
CTGACCTCCAAGATCAAGCTGGCCTTCACCTATCCGGCCATCGTCACGGTGGTGGCCTTCGCCATCGTGATCTTCCTGCT
GTCGTACGTGGTGCCGCAGGTGGTGAGCGTGTTCGCCAACACCAAGCAGAAGCTGCCGACGCTGACCATCATCATGCTGT
GGCTGTCGGATTTCGTGCGCAACTGGGGATGGCTGGCGGCGATCGTGCTGGCGGCGCTGGGGCTGCTGGTGCGCAAGCTG
CTCCAGCAGCCGGCGCTGCGGCTGTCGTGGCATCGCTGGCTGCTGACCGCGCCGCTGTTCGGCAAGCTGGTCCGCGGCTA
CAACACGGCGCGCTTTGCCAGTACGCTGGCCATCCTGAGCAGCGCCGGCGTGCCGATCCTGCGCGGCCTGCAGGCCGCCG
GGGAAACGCTCAACAATGTCGCGCTCAAGACCAACGTCGAGGATGCGGCCACGCGTGTGCGGGAAGGCACGTCGCTGGCG
CGGGCGCTGGCGGCGCAGAACCAGTTTCCGCCGGTGCTGGTGCACCTGATCCGCTCGGGCGAGGCGACCGGCAACCTGCC
GGCCATGCTGGAACGCGCGGCGCAAGGCGAGGCGCAGGAGCTGGAGCGGCGCACGCTGTTCCTGACCGGGCTGCTCGAAC
CCGCGCTGATCCTGACGATGGGGGTGGTGGTGCTGCTGATCGTGCTGGCGGTGCTGATGCCGATCATCGAGATCAATCAG
CTGGTGCACTGA

Upstream 100 bases:

>100_bases
ATCGGCCAGGGCATGCATACCATGCGCGCGGATGCGCAGCGGTGGCTGGACGCGGGCGCGACGTCGCTGGAAGAGGTGCT
GCGCGTGACGCGCGATTGAT

Downstream 100 bases:

>100_bases
TGGGGGCCGCACCTGCTCCCAACGTCACGTCCGTCGGGGAACACGCCTCTGAGACGTTTCAAACAACAGGTCACGCCGAA
AAATTGGGTGCGACCGGGAC

Product: general secretion pathway protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MPAFRYEAADASGRTDRGVIEADSARQARTALRARGLTPLVVDALGAQAAKRAGSRFGKRLSPQENALVTRQLASLLVAG
LPLDEALAALADQAERPYVHELLASIRAEVVGGSSLSVALAQHPRDFPDIYRALVSAGEHSGNLGLVLSRLADYIESRNA
LTSKIKLAFTYPAIVTVVAFAIVIFLLSYVVPQVVSVFANTKQKLPTLTIIMLWLSDFVRNWGWLAAIVLAALGLLVRKL
LQQPALRLSWHRWLLTAPLFGKLVRGYNTARFASTLAILSSAGVPILRGLQAAGETLNNVALKTNVEDAATRVREGTSLA
RALAAQNQFPPVLVHLIRSGEATGNLPAMLERAAQGEAQELERRTLFLTGLLEPALILTMGVVVLLIVLAVLMPIIEINQ
LVH

Sequences:

>Translated_403_residues
MPAFRYEAADASGRTDRGVIEADSARQARTALRARGLTPLVVDALGAQAAKRAGSRFGKRLSPQENALVTRQLASLLVAG
LPLDEALAALADQAERPYVHELLASIRAEVVGGSSLSVALAQHPRDFPDIYRALVSAGEHSGNLGLVLSRLADYIESRNA
LTSKIKLAFTYPAIVTVVAFAIVIFLLSYVVPQVVSVFANTKQKLPTLTIIMLWLSDFVRNWGWLAAIVLAALGLLVRKL
LQQPALRLSWHRWLLTAPLFGKLVRGYNTARFASTLAILSSAGVPILRGLQAAGETLNNVALKTNVEDAATRVREGTSLA
RALAAQNQFPPVLVHLIRSGEATGNLPAMLERAAQGEAQELERRTLFLTGLLEPALILTMGVVVLLIVLAVLMPIIEINQ
LVH
>Mature_402_residues
PAFRYEAADASGRTDRGVIEADSARQARTALRARGLTPLVVDALGAQAAKRAGSRFGKRLSPQENALVTRQLASLLVAGL
PLDEALAALADQAERPYVHELLASIRAEVVGGSSLSVALAQHPRDFPDIYRALVSAGEHSGNLGLVLSRLADYIESRNAL
TSKIKLAFTYPAIVTVVAFAIVIFLLSYVVPQVVSVFANTKQKLPTLTIIMLWLSDFVRNWGWLAAIVLAALGLLVRKLL
QQPALRLSWHRWLLTAPLFGKLVRGYNTARFASTLAILSSAGVPILRGLQAAGETLNNVALKTNVEDAATRVREGTSLAR
ALAAQNQFPPVLVHLIRSGEATGNLPAMLERAAQGEAQELERRTLFLTGLLEPALILTMGVVVLLIVLAVLMPIIEINQL
VH

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=403, Percent_Identity=37.7171215880893, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1786295, Length=399, Percent_Identity=29.5739348370927, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43407; Mature: 43276

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAFRYEAADASGRTDRGVIEADSARQARTALRARGLTPLVVDALGAQAAKRAGSRFGKR
CCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
LSPQENALVTRQLASLLVAGLPLDEALAALADQAERPYVHELLASIRAEVVGGSSLSVAL
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHH
AQHPRDFPDIYRALVSAGEHSGNLGLVLSRLADYIESRNALTSKIKLAFTYPAIVTVVAF
HHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHH
AIVIFLLSYVVPQVVSVFANTKQKLPTLTIIMLWLSDFVRNWGWLAAIVLAALGLLVRKL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQQPALRLSWHRWLLTAPLFGKLVRGYNTARFASTLAILSSAGVPILRGLQAAGETLNNV
HHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ALKTNVEDAATRVREGTSLARALAAQNQFPPVLVHLIRSGEATGNLPAMLERAAQGEAQE
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
LERRTLFLTGLLEPALILTMGVVVLLIVLAVLMPIIEINQLVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAFRYEAADASGRTDRGVIEADSARQARTALRARGLTPLVVDALGAQAAKRAGSRFGKR
CCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
LSPQENALVTRQLASLLVAGLPLDEALAALADQAERPYVHELLASIRAEVVGGSSLSVAL
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHH
AQHPRDFPDIYRALVSAGEHSGNLGLVLSRLADYIESRNALTSKIKLAFTYPAIVTVVAF
HHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHH
AIVIFLLSYVVPQVVSVFANTKQKLPTLTIIMLWLSDFVRNWGWLAAIVLAALGLLVRKL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQQPALRLSWHRWLLTAPLFGKLVRGYNTARFASTLAILSSAGVPILRGLQAAGETLNNV
HHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ALKTNVEDAATRVREGTSLARALAAQNQFPPVLVHLIRSGEATGNLPAMLERAAQGEAQE
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
LERRTLFLTGLLEPALILTMGVVVLLIVLAVLMPIIEINQLVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]