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The map label for this gene is gspE [H]

Identifier: 17547834

GI number: 17547834

Start: 3352592

End: 3354193

Strand: Direct

Name: gspE [H]

Synonym: RSc3115

Alternate gene names: 17547834

Gene position: 3352592-3354193 (Clockwise)

Preceding gene: 17547833

Following gene: 17547835

Centisome position: 90.21

GC content: 70.91

Gene sequence:

>1602_bases
ATGGCGGCTGAGACGATCCACGCGGCCGGAAAGGCGGTGAACATCATGGCAACGCAAGCTTCTTCCACTGACGCGCGCAC
GCTCGAGCCGCCGTCGCCCTCGGCGGTGCGGCTGGTGCCGTATGTGTTCGCGCGCGACGCCAGGCTGCTGGTGGCGCGGC
AGGATGTCGACTCGCTCGAGGTGTGGGTCTGCCCCGAGACCTCGCGCGCCGCGCTGGCCGAGCTGGCCCGCGTGTTCGGC
GCGCTGCGCCTGGTGAGCCTGGACGCCGCCGCGCTCTCCGCCGCCACGCAGACGGCCTACAACGCCCAGGACGGCAGCGC
GGCCCAGGTGGTCGGCGAGGTCGAGGGCGAGGTGGACCTGTCGCGCCTGATGCAGGACATCCCGGCCGTGGAAGACCTGC
TCGAATCGGAAGACGACGCGCCGATCATCCGCATGATCAACGCGCTGCTCACGCAGGCCGCACGCGAGGGCGCCTCGGAC
ATCCACATCGAGCCGTTCGAGAACGCCTCGGTGGTGCGCTTCCGCGTGGACGGCACGCTGCGCGACGTGGTGCGCCCGAA
GAAGGCGCTGCACGGCGCGCTGATCTCGCGCATCAAGATCATGGCGCAGCTCGACATCGCCGAGAAACGCCTGCCGCAGG
ACGGCCGCATCACGCTGCGCGTGGGCGGGCGACCGGTGGACGTGCGTGTCTCCACCCTGCCGACCGGCCACGGCGAGCGC
GCGGTGCTGCGCCTGCTCGACAAGGAAGCCGGCCGGCTTGACCTCGGCAAGCTCGGCATGGGCGCCGGCACGCTGGGGCG
CTTCGACCGCCTGATCAACCAGCCGCACGGCATCGTGCTCGTCACCGGCCCGACCGGCTCGGGCAAGACCACCACGCTGT
ATGCCGCGCTGTCGCGGCTCGATGCCAGCAGCACCAACATCATGACGGTGGAGGACCCGATCGAGTACGACCTGGACGGT
ATCGGCCAGACCCAGGTCAACGCGAAGATCGACATGACCTTCGCCAAGGCCCTGCGCGCCATCCTGCGGCAGGACCCGGA
CGTGGTGATGATCGGCGAGATCCGCGACCTGGAGACCGCGCAGATCGCGGTGCAGGCCTCGCTGACCGGCCACCTGGTGC
TGGCGACGCTGCACACCAACGATTCGGCCTCGGCGGTCACGCGGCTGATCGACATGGGGATCGAGCCGTTCCTGCTGTCG
TCGTCGCTGCTGGGCGTGCTGGCGCAGCGGCTGGTGCGGCGCCTGTGCGTGCACTGCCGCCGCGAGGAAGTGCTGGAGCT
CACCCCCACCGAGATCGAGGCCGTGGCCGGCACGCCGCCCGCCGACGGCACCGCCGCCGCCGTGCCGACGCGCCGCTCGG
TGTGGCACCACGTCGGCTGCGAGCACTGCGGCCAATCGGGCTACCAGGGCCGGACCGGCGTGTACGAGCTGCTGACGGTG
ACGCCCGAGATCCAGACCATGATCCACCGCCAGGCGCCCGAGTCCGAGATCAAGGCGCTCGCCATCGGCCAGGGCATGCA
TACCATGCGCGCGGATGCGCAGCGGTGGCTGGACGCGGGCGCGACGTCGCTGGAAGAGGTGCTGCGCGTGACGCGCGATT
GA

Upstream 100 bases:

>100_bases
CCCGGCGCACCGATGCCGCAGGGGACCACGCGCAGCGAGCCGCGCCTGCAGAACTTCGCGCCGCCCACCTCGGGCGGGTA
TGACGGCAACGCGCCGCGCA

Downstream 100 bases:

>100_bases
TATGCCAGCCTTCCGCTACGAAGCCGCCGACGCCTCCGGCCGCACCGACCGCGGCGTCATCGAAGCCGACAGCGCCCGCC
AGGCGCGCACCGCGCTGCGC

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Pullulanase secretion protein pulE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MAAETIHAAGKAVNIMATQASSTDARTLEPPSPSAVRLVPYVFARDARLLVARQDVDSLEVWVCPETSRAALAELARVFG
ALRLVSLDAAALSAATQTAYNAQDGSAAQVVGEVEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASD
IHIEPFENASVVRFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGER
AVLRLLDKEAGRLDLGKLGMGAGTLGRFDRLINQPHGIVLVTGPTGSGKTTTLYAALSRLDASSTNIMTVEDPIEYDLDG
IGQTQVNAKIDMTFAKALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDSASAVTRLIDMGIEPFLLS
SSLLGVLAQRLVRRLCVHCRREEVLELTPTEIEAVAGTPPADGTAAAVPTRRSVWHHVGCEHCGQSGYQGRTGVYELLTV
TPEIQTMIHRQAPESEIKALAIGQGMHTMRADAQRWLDAGATSLEEVLRVTRD

Sequences:

>Translated_533_residues
MAAETIHAAGKAVNIMATQASSTDARTLEPPSPSAVRLVPYVFARDARLLVARQDVDSLEVWVCPETSRAALAELARVFG
ALRLVSLDAAALSAATQTAYNAQDGSAAQVVGEVEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASD
IHIEPFENASVVRFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGER
AVLRLLDKEAGRLDLGKLGMGAGTLGRFDRLINQPHGIVLVTGPTGSGKTTTLYAALSRLDASSTNIMTVEDPIEYDLDG
IGQTQVNAKIDMTFAKALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDSASAVTRLIDMGIEPFLLS
SSLLGVLAQRLVRRLCVHCRREEVLELTPTEIEAVAGTPPADGTAAAVPTRRSVWHHVGCEHCGQSGYQGRTGVYELLTV
TPEIQTMIHRQAPESEIKALAIGQGMHTMRADAQRWLDAGATSLEEVLRVTRD
>Mature_532_residues
AAETIHAAGKAVNIMATQASSTDARTLEPPSPSAVRLVPYVFARDARLLVARQDVDSLEVWVCPETSRAALAELARVFGA
LRLVSLDAAALSAATQTAYNAQDGSAAQVVGEVEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDI
HIEPFENASVVRFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERA
VLRLLDKEAGRLDLGKLGMGAGTLGRFDRLINQPHGIVLVTGPTGSGKTTTLYAALSRLDASSTNIMTVEDPIEYDLDGI
GQTQVNAKIDMTFAKALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDSASAVTRLIDMGIEPFLLSS
SLLGVLAQRLVRRLCVHCRREEVLELTPTEIEAVAGTPPADGTAAAVPTRRSVWHHVGCEHCGQSGYQGRTGVYELLTVT
PEIQTMIHRQAPESEIKALAIGQGMHTMRADAQRWLDAGATSLEEVLRVTRD

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=472, Percent_Identity=52.3305084745763, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1786296, Length=421, Percent_Identity=43.4679334916865, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI87082188, Length=195, Percent_Identity=42.5641025641026, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 57341; Mature: 57210

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAETIHAAGKAVNIMATQASSTDARTLEPPSPSAVRLVPYVFARDARLLVARQDVDSLE
CCCCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEHHHHHCCCEEEEEECCCCCEE
VWVCPETSRAALAELARVFGALRLVSLDAAALSAATQTAYNAQDGSAAQVVGEVEGEVDL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHH
SRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDIHIEPFENASVVRFRVDGTL
HHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEECCHH
RDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHH
AVLRLLDKEAGRLDLGKLGMGAGTLGRFDRLINQPHGIVLVTGPTGSGKTTTLYAALSRL
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHC
DASSTNIMTVEDPIEYDLDGIGQTQVNAKIDMTFAKALRAILRQDPDVVMIGEIRDLETA
CCCCCCEEEECCCCCCCCCCCCCEEECCEEEHHHHHHHHHHHHCCCCEEEECCCCCCCCE
QIAVQASLTGHLVLATLHTNDSASAVTRLIDMGIEPFLLSSSLLGVLAQRLVRRLCVHCR
EEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
REEVLELTPTEIEAVAGTPPADGTAAAVPTRRSVWHHVGCEHCGQSGYQGRTGVYELLTV
HHHHHCCCCCHHHEECCCCCCCCCEECCCCHHHHHHHCCHHHHCCCCCCCCCCCEEEEEC
TPEIQTMIHRQAPESEIKALAIGQGMHTMRADAQRWLDAGATSLEEVLRVTRD
CHHHHHHHHHCCCHHHHHEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AAETIHAAGKAVNIMATQASSTDARTLEPPSPSAVRLVPYVFARDARLLVARQDVDSLE
CCCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEHHHHHCCCEEEEEECCCCCEE
VWVCPETSRAALAELARVFGALRLVSLDAAALSAATQTAYNAQDGSAAQVVGEVEGEVDL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHH
SRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDIHIEPFENASVVRFRVDGTL
HHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEECCHH
RDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHH
AVLRLLDKEAGRLDLGKLGMGAGTLGRFDRLINQPHGIVLVTGPTGSGKTTTLYAALSRL
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHC
DASSTNIMTVEDPIEYDLDGIGQTQVNAKIDMTFAKALRAILRQDPDVVMIGEIRDLETA
CCCCCCEEEECCCCCCCCCCCCCEEECCEEEHHHHHHHHHHHHCCCCEEEECCCCCCCCE
QIAVQASLTGHLVLATLHTNDSASAVTRLIDMGIEPFLLSSSLLGVLAQRLVRRLCVHCR
EEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
REEVLELTPTEIEAVAGTPPADGTAAAVPTRRSVWHHVGCEHCGQSGYQGRTGVYELLTV
HHHHHCCCCCHHHEECCCCCCCCCEECCCCHHHHHHHCCHHHHCCCCCCCCCCCEEEEEC
TPEIQTMIHRQAPESEIKALAIGQGMHTMRADAQRWLDAGATSLEEVLRVTRD
CHHHHHHHHHCCCHHHHHEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1738317; 2677007 [H]