Click here to switch to the map view.

The map label for this gene is kefF [H]

Identifier: 17547816

GI number: 17547816

Start: 3335279

End: 3335827

Strand: Reverse

Name: kefF [H]

Synonym: RSc3097

Alternate gene names: 17547816

Gene position: 3335827-3335279 (Counterclockwise)

Preceding gene: 17547820

Following gene: 17547815

Centisome position: 89.76

GC content: 70.13

Gene sequence:

>549_bases
ATGCATCCGCCGCGCATTCTCGTGATCTACGCGCATCCCGCGCCGCGCCGCTCGCGCGCCAACAAGCCGCTGGCGCGCAT
GCTGGCCGGCCTGCCCGGCGTGGCCCTGCACGATCTCTACTGGCACTACCCCGAGTTCGACATCGACGTGCGCGCCGAGC
AACAGGCGCTGGAAGCCGCCGACCTGGTGGTCCTCCAGTTTCCGGTGCAGTGGTATGCCACGCCATCGCTGCTCAAGGAG
TGGCTGGATGTCGTGCTGGAGATGGGCTGGGCCTACGGGCCGGGCGGCACGGCCCTGCGAGGCAAGCACATGCTGGTGCT
GGCGACCACCGGCGGCCGCGCACACGCCTACAGTCGGGACGGCATCCACGGCCACGCCTTCGACCTGTTCCTGTTGCCCT
TGCAGCAGACCGCCGCCCTGTGCGGCATGCACTGGCTGCCGCCGCACGTGCTGCACGACGCCGACGACGCCTCGCCCGAA
GTGCTCGACGCCCATCTCGAGCAGGTACGCGCCAGCCTGCAGCCATGGCTGGCGGCTGTACCCGCCTGA

Upstream 100 bases:

>100_bases
GCACCACCCGATCCGGCAATCCACGCCCTCGCGCCGCGCCACGATAGCCGTGCGAAGATGCGTCATCAAGACGCACCGCC
TCCTGACTTTCCCGATCCCC

Downstream 100 bases:

>100_bases
TTTCGGCCTGACCCTTCTTCTCGATTCCGCTCCATGCAATCGCACGATCTGCTCGCCAATTTCGTCATCTACCTGGCTGC
GGCGGTGGCCGTCGTACCCC

Product: NAD(P)H oxidoreductase (NAD(P)H dehydrogenase (quinone)) oxidoreductase

Products: NAD+; NADP+; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MHPPRILVIYAHPAPRRSRANKPLARMLAGLPGVALHDLYWHYPEFDIDVRAEQQALEAADLVVLQFPVQWYATPSLLKE
WLDVVLEMGWAYGPGGTALRGKHMLVLATTGGRAHAYSRDGIHGHAFDLFLLPLQQTAALCGMHWLPPHVLHDADDASPE
VLDAHLEQVRASLQPWLAAVPA

Sequences:

>Translated_182_residues
MHPPRILVIYAHPAPRRSRANKPLARMLAGLPGVALHDLYWHYPEFDIDVRAEQQALEAADLVVLQFPVQWYATPSLLKE
WLDVVLEMGWAYGPGGTALRGKHMLVLATTGGRAHAYSRDGIHGHAFDLFLLPLQQTAALCGMHWLPPHVLHDADDASPE
VLDAHLEQVRASLQPWLAAVPA
>Mature_182_residues
MHPPRILVIYAHPAPRRSRANKPLARMLAGLPGVALHDLYWHYPEFDIDVRAEQQALEAADLVVLQFPVQWYATPSLLKE
WLDVVLEMGWAYGPGGTALRGKHMLVLATTGGRAHAYSRDGIHGHAFDLFLLPLQQTAALCGMHWLPPHVLHDADDASPE
VLDAHLEQVRASLQPWLAAVPA

Specific function: Required for full activity of kefC [H]

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily [H]

Homologues:

Organism=Homo sapiens, GI156564357, Length=133, Percent_Identity=38.3458646616541, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI4505415, Length=129, Percent_Identity=37.2093023255814, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1786231, Length=174, Percent_Identity=47.1264367816092, Blast_Score=153, Evalue=6e-39,
Organism=Escherichia coli, GI1789750, Length=164, Percent_Identity=40.8536585365854, Blast_Score=129, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.2

Molecular weight: Translated: 20188; Mature: 20188

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPPRILVIYAHPAPRRSRANKPLARMLAGLPGVALHDLYWHYPEFDIDVRAEQQALEAA
CCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCCCEECCHHHHHHHHH
DLVVLQFPVQWYATPSLLKEWLDVVLEMGWAYGPGGTALRGKHMLVLATTGGRAHAYSRD
CEEEEEECHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEEECCCCCCCCCCC
GIHGHAFDLFLLPLQQTAALCGMHWLPPHVLHDADDASPEVLDAHLEQVRASLQPWLAAV
CCCCCCCEEEEECHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PA
CC
>Mature Secondary Structure
MHPPRILVIYAHPAPRRSRANKPLARMLAGLPGVALHDLYWHYPEFDIDVRAEQQALEAA
CCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCCCEECCHHHHHHHHH
DLVVLQFPVQWYATPSLLKEWLDVVLEMGWAYGPGGTALRGKHMLVLATTGGRAHAYSRD
CEEEEEECHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEEECCCCCCCCCCC
GIHGHAFDLFLLPLQQTAALCGMHWLPPHVLHDADDASPEVLDAHLEQVRASLQPWLAAV
CCCCCCCEEEEECHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; NADPH; H+; acceptor

Specific reaction: NAD(P)H + H+ + acceptor = NAD(P)+ + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA